EKS-MUM-00404

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00404

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	425.2	2.0E-127	235	484	250

Status

Reviewed

Ensembl Protein

ENSMUSP00000014597

UniProt Accession

P16277; Q8K2M8;

Protein Name

Tyrosine-protein kinase Blk

Protein Synonyms/Alias

B lymphocyte kinase; p55-Blk;

Gene Name

Blk

Gene Synonyms/Alias

Blk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000014453	ENSMUSP00000014597	ENSMUST00000014597

Organism

Mus musculus

Functional Description

Non-receptor tyrosine kinase involved in B-lymphocytedevelopment, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulines and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr- 207'. Phosphorylates also the immunoglobuline G receptor FCGR2. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.

Protein Length

499

Protein Sequence
(FASTA)

MGLLSSKRQV SEKGKGWSPV KIRTQDKAPP PLPPLVVFNH LAPPSPNQDP DEEERFVVAL 60

FDYAAVNDRD LQVLKGEKLQ VLRSTGDWWL ARSLVTGREG YVPSNFVAPV ETLEVEKWFF 120

RTISRKDAER QLLAPMNKAG SFLIRESESN KGAFSLSVKD ITTQGEVVKH YKIRSLDNGG 180

YYISPRITFP TLQALVQHYS KKGDGLCQKL TLPCVNLAPK NLWAQDEWEI PRQSLKLVRK 240

LGSGQFGEVW MGYYKNNMKV AIKTLKEGTM SPEAFLGEAN VMKTLQHERL VRLYAVVTRE 300

PIYIVTEYMA RGCLLDFLKT DEGSRLSLPR LIDMSAQVAE GMAYIERMNS IHRDLRAANI 360

LVSETLCCKI ADFGLARIID SEYTAQEGAK FPIKWTAPEA IHFGVFTIKA DVWSFGVLLM 420

EIVTYGRVPY PGMSNPEVIR SLEHGYRMPC PETCPPELYN DIITECWRGR PEERPTFEFL 480

QSVLEDFYTA TEGQYELQP 499

Nucleotide Sequence
(FASTA)

ATGGGGCTGC TGAGCAGCAA GAGGCAGGTC AGTGAGAAGG GCAAGGGCTG GAGCCCCGTG 60

AAGATCCGCA CCCAGGACAA GGCTCCCCCA CCCCTGCCAC CCCTGGTTGT CTTCAACCAC 120

CTTGCCCCAC CATCTCCTAA CCAGGACCCA GATGAAGAGG AGCGTTTTGT GGTGGCTCTG 180

TTTGACTATG CCGCTGTGAA TGACAGGGAC CTTCAGGTGC TGAAGGGTGA GAAGCTCCAG 240

GTCTTGAGGA GCACTGGAGA CTGGTGGTTG GCCAGGTCAC TCGTCACAGG AAGAGAAGGT 300

TATGTGCCCA GCAACTTTGT GGCCCCAGTA GAGACTCTGG AAGTAGAAAA ATGGTTCTTC 360

AGGACCATCA GCCGGAAGGA TGCTGAGAGG CAGTTGCTGG CTCCGATGAA CAAGGCCGGC 420

TCCTTTCTCA TCAGAGAGAG TGAGAGCAAT AAAGGTGCCT TTTCCCTGTC CGTGAAAGAT 480

ATCACCACCC AGGGGGAGGT GGTCAAGCAC TATAAGATCC GATCACTGGA CAATGGAGGC 540

TATTACATCT CCCCCCGGAT CACCTTTCCC ACCCTCCAGG CCCTGGTGCA GCACTATTCA 600

AAGAAAGGGG ATGGTTTGTG TCAGAAGTTG ACTCTGCCCT GTGTGAACCT GGCCCCGAAG 660

AACCTTTGGG CCCAAGATGA ATGGGAAATC CCCAGGCAGT CTCTCAAGTT GGTCCGGAAA 720

CTTGGGTCTG GGCAGTTTGG CGAAGTCTGG ATGGGTTATT ACAAAAATAA CATGAAGGTG 780

GCCATCAAGA CCCTGAAGGA GGGAACCATG TCACCGGAAG CTTTCCTGGG CGAGGCCAAC 840

GTGATGAAAA CCCTGCAGCA TGAGAGGCTG GTTCGTCTCT ACGCTGTGGT CACCAGAGAG 900

CCCATTTACA TCGTCACTGA ATACATGGCC AGAGGATGCT TGCTGGATTT TCTGAAGACC 960

GATGAAGGTA GCAGGTTGTC CCTTCCAAGG CTGATTGACA TGTCAGCCCA GGTTGCAGAG 1020

GGGATGGCTT ACATAGAGCG CATGAATTCC ATCCACCGTG ACCTGCGGGC AGCCAACATC 1080

CTGGTGTCTG AGACGTTGTG CTGCAAAATC GCTGACTTCG GCTTGGCCAG GATCATTGAC 1140

AGTGAATACA CTGCCCAAGA GGGGGCCAAG TTCCCCATCA AGTGGACCGC CCCGGAGGCC 1200

ATCCACTTCG GGGTGTTTAC CATCAAGGCT GATGTGTGGT CCTTCGGAGT CTTGCTGATG 1260

GAGATTGTCA CCTATGGGCG CGTTCCCTAC CCAGGAATGA GCAACCCTGA GGTCATCCGT 1320

AGCCTGGAGC ACGGCTACCG AATGCCATGC CCGGAGACAT GTCCACCGGA GTTGTACAAT 1380

GATATCATCA CTGAGTGCTG GCGGGGCCGG CCAGAGGAGC GGCCTACCTT TGAGTTCCTG 1440

CAGTCGGTGT TGGAGGACTT CTACACAGCC ACGGAGGGCC AATATGAGCT GCAGCCCTAG 1500

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         lklv+klG+GqfGevw+g++k+++kva+ktlkegt+speafl ea+++k+l+he+lv+ly

Q: 235   LKLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKTLQHERLVRLY  294

         689*********************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avv++ ePiyivte+ma+G+lldflk++eg++l+lp+l+d++aqvaeGmay+e++n ihr

Q: 295   AVVTR-EPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQVAEGMAYIERMNSIHR  353

         *****.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+a+n+lv+e+l++k+adfGlar+i d+eyta+egak+PikWtaPeai++g+ftik+dv

Q: 354   DLRAANILVSETLCCKIADFGLARII-DSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADV  412

         **************************.899******************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelye.lllecwkkdpe  239

         WsfG+ll+eivt+G+vPypGm+n ev++++e+gyr+p+pe+cp ely+ +++ecw+ +pe

Q: 413   WSFGVLLMEIVTYGRVPYPGMSNPEVIRSLEHGYRMPCPETCPPELYNdIITECWRGRPE  472

         ***********************************************99***********

S: 240   erPtfetlqevl  251

         erPtfe+lq+vl

Q: 473   ERPTFEFLQSVL  484

         ********9986

Domain Sequence
(FASTA)

LKLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKT LQHERLVRLY 60

AVVTREPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQVAEGMAY IERMNSIHRD 120

LRAANILVSE TLCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS 180

FGVLLMEIVT YGRVPYPGMS NPEVIRSLEH GYRMPCPETC PPELYNDIIT ECWRGRPEER 240

PTFEFLQSVL 250

Keyword

3D-structure; ATP-binding; Cell membrane; Complete proteome; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tursiops truncatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0050853	; P:B cell receptor signaling pathway
GO:0032024	; P:positive regulation of insulin secretion

KEGG

mmu:12143

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; FALSE_NEG.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013