EKS-MUM-00399

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00399

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Src	447.3	3.5E-134	275	524	250

Status

Reviewed

Ensembl Protein

ENSMUSP00000072154

UniProt Accession

Q04736;

Protein Name

Tyrosine-protein kinase Yes

Protein Synonyms/Alias

Proto-oncogene c-Yes; p61-Yes;

Gene Name

Yes1

Gene Synonyms/Alias

Yes1; Yes;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000014932	ENSMUSP00000132161	ENSMUST00000168707
ENSMUSG00000014932	ENSMUSP00000072154	ENSMUST00000072311

Organism

Mus musculus

Functional Description

Non-receptor protein tyrosine kinase that is involved inthe regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).

Protein Length

541

Protein Sequence
(FASTA)

MGCIKSKENK SPAIKYTPEN LTEPVSPSAS HYGVEHATVA PTSSTKGASV NFNSLSMTPF 60

GGSSGVTPFG GASSSFSVVS SSYPTGLTGG VTIFVALYDY EARTTEDLSF KKGERFQIIN 120

NTEGDWWEAR SIATGKSGYI PSNYVVPADS IQAEEWYFGK MGRKDAERLL LNPGNQRGIF 180

LVRESETTKG AYSLSIRDWD EVRGDNVKHY KIRKLDNGGY YITTRAQFDT LQKLVKHYTE 240

HADGLCHKLT TVCPTVKPQT QGLAKDAWEI PRESLRLEVK LGQGCFGEVW MGTWNGTTKV 300

AIKTLKPGTM MPEAFLQEAQ IMKKLRHDKL VPLYAVVSEE PIYIVTEFMS KGSLLDFLKE 360

GDGKYLKLPQ LVDMAAQIAD GMAYIERMNY IHRDLRAANI LVGENLICKI ADFGLARLIE 420

DNEYTARQGA KFPIKWTAPE AALYGRFTIK SDVWSFGILQ TELVTKGRVP YPGMVNREVL 480

EQVERGYRMP CPQGCPESLH ELMNLCWKKD PDERPTFEYI QSFLEDYFTA TEPQYQPGEN 540

L 541

Nucleotide Sequence
(FASTA)

ATGGGCTGCA TTAAAAGTAA AGAAAACAAA AGTCCAGCCA TAAAATACAC ACCGGAAAAT 60

CTTACAGAGC CTGTAAGCCC AAGTGCCAGT CATTATGGAG TGGAACATGC TACAGTTGCC 120

CCGACCTCTT CCACAAAGGG AGCATCAGTT AATTTTAACA GTCTTTCCAT GACACCCTTT 180

GGAGGGTCCT CAGGGGTGAC TCCTTTTGGA GGAGCGTCTT CCTCATTCTC AGTGGTGTCA 240

AGTTCATATC CTACAGGTTT AACAGGTGGT GTCACTATAT TTGTGGCCTT GTATGATTAT 300

GAAGCTAGAA CTACAGAAGA CCTTTCCTTT AAGAAGGGTG AACGATTTCA AATAATTAAC 360

AATACGGAAG GAGACTGGTG GGAAGCAAGA TCAATTGCTA CCGGAAAGAG TGGTTATATC 420

CCTAGCAATT ACGTAGTGCC TGCAGATTCC ATTCAGGCAG AAGAATGGTA TTTTGGCAAA 480

ATGGGGAGAA AAGATGCGGA AAGATTACTT CTGAATCCTG GGAATCAGCG AGGTATTTTC 540

TTAGTAAGAG AAAGTGAAAC TACTAAAGGT GCTTACTCCC TCTCAATCCG TGATTGGGAT 600

GAGGTGAGGG GTGACAATGT GAAGCATTAC AAGATCAGAA AACTTGACAA TGGTGGCTAC 660

TACATCACGA CCAGAGCTCA GTTTGATACA CTGCAGAAGC TGGTGAAGCA CTACACAGAA 720

CATGCTGATG GATTATGCCA CAAGTTAACA ACTGTGTGTC CTACTGTGAA ACCCCAGACT 780

CAAGGTCTGG CAAAAGATGC TTGGGAAATC CCTCGAGAAT CATTGCGACT AGAGGTGAAA 840

CTAGGTCAAG GATGCTTTGG GGAAGTGTGG ATGGGAACAT GGAATGGAAC TACAAAAGTA 900

GCAATCAAAA CACTAAAGCC AGGTACAATG ATGCCAGAAG CATTCCTTCA AGAAGCTCAG 960

ATAATGAAAA AGCTAAGACA CGATAAACTT GTTCCACTCT ATGCAGTTGT TTCTGAAGAG 1020

CCCATTTATA TTGTCACCGA GTTTATGTCA AAAGGAAGCT TGTTAGATTT CCTTAAAGAA 1080

GGAGATGGAA AGTATTTGAA GCTTCCACAG CTGGTTGATA TGGCTGCTCA GATCGCTGAT 1140

GGCATGGCGT ATATTGAAAG AATGAACTAT ATTCACCGAG ATCTCCGAGC TGCTAATATT 1200

CTTGTAGGAG AAAATCTTAT ATGCAAAATA GCAGATTTTG GCTTAGCAAG ATTAATTGAA 1260

GACAATGAAT ACACGGCAAG ACAAGGTGCA AAATTTCCAA TCAAGTGGAC AGCTCCTGAG 1320

GCTGCTCTGT ATGGTCGATT TACAATAAAG TCAGATGTGT GGTCATTTGG AATTCTACAG 1380

ACAGAGCTGG TAACAAAAGG AAGAGTGCCA TATCCAGGTA TGGTAAACCG TGAAGTATTG 1440

GAACAAGTAG AGCGGGGATA CAGAATGCCT TGCCCCCAGG GCTGTCCCGA ATCCCTCCAT 1500

GAATTGATGA ATCTTTGCTG GAAGAAGGAT CCTGATGAAA GACCAACATT TGAATATATT 1560

CAGTCCTTCT TGGAAGACTA CTTCACTGCT ACAGAGCCAC AGTACCAACC AGGAGAAAAT 1620

TTATAA 1626

Domain Profile

S: 1     lklvkklGeGqfGevwlgkwkgsvkvavktlkegtlspeafleeaqilkklrheklvkly  60

         l+l+ klG+G+fGevw+g+w+g++kva+ktlk+gt++peafl+eaqi+kklrh+klv ly

Q: 275   LRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLY  334

         68999*******************************************************

S: 61    avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr  120

         avvse ePiyivte+m+kGslldflke +gk+lklp+lvd+aaq+a+Gmay+e++n+ihr

Q: 335   AVVSE-EPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHR  393

         ****9.******************************************************

S: 121   dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv  180

         dl+a+n+lvge+l++k+adfGlarlied+eyta++gak+PikWtaPea+ +g+ftiksdv

Q: 394   DLRAANILVGENLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDV  453

         ************************************************************

S: 181   WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee  240

         WsfGil te+vtkG+vPypGm n+evleqvergyr+p+p+ cpe+l+el++ cwkkdp+e

Q: 454   WSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDE  513

         ************************************************************

S: 241   rPtfetlqevl  251

         rPtfe++q++l

Q: 514   RPTFEYIQSFL  524

         *******9986

Domain Sequence
(FASTA)

LRLEVKLGQG CFGEVWMGTW NGTTKVAIKT LKPGTMMPEA FLQEAQIMKK LRHDKLVPLY 60

AVVSEEPIYI VTEFMSKGSL LDFLKEGDGK YLKLPQLVDM AAQIADGMAY IERMNYIHRD 120

LRAANILVGE NLICKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 180

SFGILQTELV TKGRVPYPGM VNREVLEQVE RGYRMPCPQG CPESLHELMN LCWKKDPDER 240

PTFEYIQSFL 250

Keyword

3D-structure; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Proto-oncogene; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005794	; C:Golgi apparatus
GO:0005815	; C:microtubule organizing center
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0015758	; P:glucose transport

KEGG

mmu:22612

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013