EKS-MUM-00325

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00325

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/IKK	441.9	2.3E-132	9	293	285

Status

Reviewed

Ensembl Protein

ENSMUSP00000020316

UniProt Accession

Q9WUN2; Q9CT90; Q9DC03;

Protein Name

Serine/threonine-protein kinase TBK1

Protein Synonyms/Alias

T2K; TANK-binding kinase 1;

Gene Name

Tbk1

Gene Synonyms/Alias

Tbk1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000020115	ENSMUSP00000020316	ENSMUST00000020316

Organism

Mus musculus

Functional Description

Serine/threonine kinase that plays an essential role inregulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFN- alpha and IFN-beta. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS or SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating IKKA/CHUK or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates and activates AKT1 (By similarity).

Protein Length

729

Protein Sequence
(FASTA)

MQSTSNHLWL LSDILGQGAT ANVFRGRHKK TGDLYAVKVF NNISFLRPVD VQMREFEVLK 60

KLNHKNIVKL FAIEEETTTR HKVLIMEFCP CGSLYTVLEE PSNAYGLPES EFLIVLRDVV 120

GGMNHLRENG IVHRDIKPGN IMRVIGEDGQ SVYKLTDFGA ARELEDDEQF VSLYGTEEYL 180

HPDMYERAVL RKDHQKKYGA TVDLWSVGVT FYHAATGSLP FRPFEGPRRN KEVMYKIITG 240

KPSGAISGVQ KAENGPIDWS GDMPLSCSLS QGLQALLTPV LANILEADQE KCWGFDQFFA 300

ETSDVLHRMV IHVFSLQHMT AHKIYIHSYN TAAVFHELVY KQTKIVSSNQ ELIYEGRRLV 360

LELGRLAQHF PKTTEENPIF VTSREQLNTV GLRYEKISLP KIHPRYDLDG DASMAKAVTG 420

VVCYACRTAS TLLLYQELMR KGVRWLVELV KDDYNETVHK KTEVVITLDF CIRNIEKTVK 480

VYEKLMKVNL EAAELGEISD IHTKLLRLSS SQGTIESSLQ DISSRLSPGG LLADTWAHQE 540

GTHPRDRNVE KLQVLLNCIT EIYYQFKKDK AERRLAYNEE QIHKFDKQKL YYHATKAMSH 600

FSEECVRKYE AFKDKSEEWM RKMLHLRKQL LSLTNQCFDI EEEVSKYQDY TNELQETLPQ 660

KMLAASGGVK HAMAPIYPSS NTLVEMTLGM KKLKEEMEGV VKELAENNHI LERFGSLTMD 720

GGLRNVDCL 729

Nucleotide Sequence
(FASTA)

ATGCAGAGCA CCTCCAACCA TCTGTGGCTC CTGTCTGATA TCCTAGGCCA GGGGGCCACT 60

GCAAATGTCT TCCGAGGAAG GCATAAGAAA ACTGGTGATC TCTATGCTGT CAAAGTATTT 120

AATAACATAA GCTTCCTTCG CCCAGTGGAT GTTCAAATGA GAGAATTTGA AGTGTTAAAA 180

AAACTCAATC ACAAAAACAT TGTCAAGTTA TTTGCTATTG AAGAGGAGAC AACAACAAGA 240

CATAAAGTGC TTATTATGGA GTTTTGTCCC TGTGGGAGTT TATACACTGT TCTAGAGGAG 300

CCGTCCAATG CGTATGGACT TCCAGAATCA GAATTTCTCA TTGTCTTACG AGATGTGGTG 360

GGCGGGATGA ATCATCTCCG AGAGAACGGC ATAGTGCACC GAGATATCAA GCCAGGCAAC 420

ATCATGCGCG TCATAGGGGA GGACGGCCAG TCTGTGTACA AACTCACGGA TTTCGGCGCC 480

GCTCGAGAGC TGGAGGACGA TGAGCAGTTT GTGTCTCTGT ACGGCACAGA AGAGTACCTG 540

CATCCGGACA TGTATGAAAG GGCAGTGCTA AGAAAGGACC ATCAGAAGAA GTACGGGGCT 600

ACCGTTGATC TGTGGAGTGT TGGAGTGACA TTCTACCATG CAGCCACGGG GTCGCTGCCG 660

TTTAGACCCT TCGAGGGGCC TCGGAGGAAC AAAGAAGTAA TGTATAAAAT AATCACTGGG 720

AAGCCGTCTG GTGCAATATC TGGAGTACAG AAAGCAGAAA ACGGACCAAT TGACTGGAGT 780

GGAGACATGC CTCTCTCCTG TAGTCTTTCT CAGGGTCTTC AGGCACTGCT TACCCCAGTT 840

CTTGCAAACA TACTTGAAGC TGATCAGGAG AAGTGCTGGG GTTTTGACCA GTTCTTTGCA 900

GAGACCAGTG ATGTGCTTCA CCGAATGGTG ATCCATGTCT TCTCGCTACA ACACATGACG 960

GCGCATAAGA TTTACATTCA CAGCTATAAC ACTGCTGCTG TGTTCCATGA ACTGGTCTAT 1020

AAACAAACCA AGATTGTTTC CTCAAATCAA GAACTTATCT ACGAAGGACG ACGCTTAGTC 1080

CTAGAACTCG GACGACTAGC CCAGCATTTT CCTAAAACCA CAGAGGAAAA TCCTATCTTT 1140

GTCACGAGCC GGGAACAACT CAATACCGTA GGACTGAGAT ATGAAAAAAT TTCCCTCCCT 1200

AAAATACATC CACGCTATGA TCTGGATGGG GACGCCAGCA TGGCCAAGGC AGTGACGGGG 1260

GTTGTGTGCT ACGCCTGCAG AACTGCCAGT ACCCTGCTGC TCTATCAAGA ATTAATGCGA 1320

AAGGGGGTAC GGTGGCTGGT TGAACTGGTT AAGGATGATT ACAACGAGAC CGTCCACAAG 1380

AAGACGGAGG TAGTGATCAC ACTGGATTTC TGCATCAGGA ACATTGAGAA GACTGTGAAA 1440

GTGTATGAGA AGTTGATGAA GGTCAACCTG GAAGCCGCAG AGCTGGGTGA GATTTCAGAC 1500

ATACACACCA AGCTGCTGAG ACTTTCCAGT TCTCAGGGAA CAATAGAAAG CAGTCTTCAG 1560

GACATCAGCA GCAGGCTGTC TCCAGGGGGC TTGCTGGCCG ACACCTGGGC ACATCAAGAA 1620

GGCACGCATC CAAGAGACAG GAATGTAGAA AAACTGCAGG TCCTGTTGAA CTGCATCACA 1680

GAGATTTACT ATCAGTTCAA AAAAGACAAA GCAGAACGCA GACTAGCTTA TAATGAAGAA 1740

CAGATCCACA AATTTGATAA GCAAAAATTG TATTACCATG CCACAAAAGC AATGAGCCAC 1800

TTCTCAGAAG AATGTGTTAG AAAGTATGAA GCGTTTAAAG ATAAGTCGGA AGAGTGGATG 1860

AGAAAGATGC TTCATCTTAG GAAGCAGCTG TTATCGCTAA CTAATCAGTG TTTCGATATC 1920

GAAGAGGAAG TGTCCAAGTA TCAAGACTAT ACTAACGAGT TACAAGAAAC TCTGCCTCAG 1980

AAAATGCTCG CAGCCTCCGG CGGCGTCAAG CACGCCATGG CCCCGATCTA CCCCAGCTCT 2040

AACACCTTAG TGGAGATGAC TCTTGGTATG AAGAAGTTAA AGGAGGAGAT GGAAGGCGTG 2100

GTTAAGGAGC TGGCCGAGAA CAATCATATT TTAGAAAGGT TTGGGTCTTT AACAATGGAT 2160

GGTGGCCTTC GCAATGTGGA CTGTCTTTAG 2190

Domain Profile

S: 1     WelkelLGeGafgnvikarnkktgekiavkvikeesslrprevqvrelevlrklnhkniv  60

         W l+++LG+Ga++nv+++r+kktg+ +avkv++++s+lrp++vq+re+evl+klnhkniv

Q: 9     WLLSDILGQGATANVFRGRHKKTGDLYAVKVFNNISFLRPVDVQMREFEVLKKLNHKNIV  68

         999*********************************************************

S: 61    klvdveeelnlasarlkvlvleyCsgGdlrkvLeepenayGLkesevlvvLsdvvaglry  120

         kl+++eee   +++r+kvl++e+C +G+l++vLeep+nayGL+ese+l+vL+dvv g+++

Q: 69    KLFAIEEE---TTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNH  125

         ********...*************************************************

S: 121   LrenkivhRDlkPenivllkgedgksiykltDlGaareldddekvvslvGteeYlaPely  180

         Lren+ivhRD+kP+ni++++gedg+s+ykltD+Gaarel+dde++vsl+GteeYl+P++y

Q: 126   LRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMY  185

         ************************************************************

S: 181   e........qkkytatvDlWsiGvllyeaitGslPflpfegprr....lykkitkkpaka  228

         e        qkky+atvDlWs+Gv++y+a+tGslPf+pfegprr    +yk+it kp++a

Q: 186   EravlrkdhQKKYGATVDLWSVGVTFYHAATGSLPFRPFEGPRRnkevMYKIITGKPSGA  245

         ************************************************************

S: 229   iagteka.ngevefssslPlsaslssglqeklekllanllevdeekrw  275

         i+g++ka ng++++s ++Pls+sls+glq+ l+++lan+le+d+ek+w

Q: 246   ISGVQKAeNGPIDWSGDMPLSCSLSQGLQALLTPVLANILEADQEKCW  293

         *******9***************************************9

Domain Sequence
(FASTA)

WLLSDILGQG ATANVFRGRH KKTGDLYAVK VFNNISFLRP VDVQMREFEV LKKLNHKNIV 60

KLFAIEEETT TRHKVLIMEF CPCGSLYTVL EEPSNAYGLP ESEFLIVLRD VVGGMNHLRE 120

NGIVHRDIKP GNIMRVIGED GQSVYKLTDF GAARELEDDE QFVSLYGTEE YLHPDMYERA 180

VLRKDHQKKY GATVDLWSVG VTFYHAATGS LPFRPFEGPR RNKEVMYKII TGKPSGAISG 240

VQKAENGPID WSGDMPLSCS LSQGLQALLT PVLANILEAD QEKCW 285

Keyword

Antiviral defense; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Immunity; Innate immunity; Isopeptide bond; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005524	; F:ATP binding
GO:0003676	; F:nucleic acid binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0002218	; P:activation of innate immune response
GO:0050830	; P:defense response to Gram-positive bacterium
GO:0051607	; P:defense response to virus
GO:0045087	; P:innate immune response
GO:0010629	; P:negative regulation of gene expression
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0032727	; P:positive regulation of interferon-alpha production
GO:0045359	; P:positive regulation of interferon-beta biosynthetic process
GO:0032728	; P:positive regulation of interferon-beta production
GO:0045944	; P:positive regulation of transcription from RNA polymerase II promoter

KEGG

mmu:56480

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.
PS00299	; UBIQUITIN_1; FALSE_NEG.
PS50053	; UBIQUITIN_2; FALSE_NEG.

Prints

Created Date

20-Feb-2013