EKS-MUM-00329

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00329

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/EGFR	498.3	1.0E-149	714	971	258

Status

Reviewed

Ensembl Protein

ENSMUSP00000020329

UniProt Accession

Q01279;

Protein Name

Epidermal growth factor receptor

Protein Synonyms/Alias

Gene Name

Egfr

Gene Synonyms/Alias

Egfr;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000020122	ENSMUSP00000020329	ENSMUST00000020329
ENSMUSG00000020122	ENSMUSP00000122632	ENSMUST00000125984
ENSMUSG00000020122	ENSMUSP00000099948	ENSMUST00000102884

Organism

Mus musculus

Functional Description

Receptor tyrosine kinase binding ligands of the EGFfamily and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Protein Length

1210

Protein Sequence
(FASTA)

MRPSGTARTT LLVLLTALCA AGGALEEKKV CQGTSNRLTQ LGTFEDHFLS LQRMYNNCEV 60

VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN ALYENTYALA 120

ILSNYGTNRT GLRELPMRNL QEILIGAVRF SNNPILCNMD TIQWRDIVQN VFMSNMSMDL 180

QSHPSSCPKC DPSCPNGSCW GGGEENCQKL TKIICAQQCS HRCRGRSPSD CCHNQCAAGC 240

TGPRESDCLV CQKFQDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV 300

VTDHGSCVRA CGPDYYEVEE DGIRKCKKCD GPCRKVCNGI GIGEFKDTLS INATNIKHFK 360

YCTAISGDLH ILPVAFKGDS FTRTPPLDPR ELEILKTVKE ITGFLLIQAW PDNWTDLHAF 420

ENLEIIRGRT KQHGQFSLAV VGLNITSLGL RSLKEISDGD VIISGNRNLC YANTINWKKL 480

FGTPNQKTKI MNNRAEKDCK AVNHVCNPLC SSEGCWGPEP RDCVSCQNVS RGRECVEKCN 540

ILEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGIM 600

GENNTLVWKY ADANNVCHLC HANCTYGCAG PGLQGCEVWP SGPKIPSIAT GIVGGLLFIV 660

VVALGIGLFM RRRHIVRKRT LRRLLQEREL VEPLTPSGEA PNQAHLRILK ETEFKKIKVL 720

GSGAFGTVYK GLWIPEGEKV KIPVAIKELR EATSPKANKE ILDEAYVMAS VDNPHVCRLL 780

GICLTSTVQL ITQLMPYGCL LDYVREHKDN IGSQYLLNWC VQIAKGMNYL EDRRLVHRDL 840

AARNVLVKTP QHVKITDFGL AKLLGAEEKE YHAEGGKVPI KWMALESILH RIYTHQSDVW 900

SYGVTVWELM TFGSKPYDGI PASDISSILE KGERLPQPPI CTIDVYMIMV KCWMIDADSR 960

PKFRELILEF SKMARDPQRY LVIQGDERMH LPSPTDSNFY RALMDEEDME DVVDADEYLI 1020

PQQGFFNSPS TSRTPLLSSL SATSNNSTVA CINRNGSCRV KEDAFLQRYS SDPTGAVTED 1080

NIDDAFLPVP EYVNQSVPKR PAGSVQNPVY HNQPLHPAPG RDLHYQNPHS NAVGNPEYLN 1140

TAQPTCLSSG FNSPALWIQK GSHQMSLDNP DYQQDFFPKE TKPNGIFKGP TAENAEYLRV 1200

APPSSEFIGA 1210

Nucleotide Sequence
(FASTA)

ATGCGACCCT CAGGGACCGC GAGAACCACA CTGCTGGTGT TGCTGACCGC GCTCTGCGCC 60

GCAGGTGGGG CGTTGGAGGA AAAGAAAGTC TGCCAAGGCA CAAGTAACAG GCTCACCCAA 120

CTGGGCACTT TTGAAGACCA CTTTCTGAGC CTGCAGAGGA TGTACAACAA CTGTGAAGTG 180

GTCCTTGGGA ACTTGGAAAT TACCTATGTG CAAAGGAATT ACGACCTTTC CTTCTTAAAG 240

ACCATCCAGG AGGTGGCCGG CTATGTCCTC ATTGCCCTCA ACACCGTGGA GAGAATCCCT 300

TTGGAGAACC TGCAGATCAT CAGGGGAAAT GCTCTTTATG AAAACACCTA TGCCTTAGCC 360

ATCCTGTCCA ACTATGGGAC AAACAGAACT GGGCTTAGGG AACTGCCCAT GCGGAACTTA 420

CAGGAAATCC TGATTGGTGC TGTGCGATTC AGCAACAACC CCATCCTCTG CAATATGGAT 480

ACTATCCAGT GGAGGGACAT CGTCCAAAAC GTCTTTATGA GCAACATGTC AATGGACTTA 540

CAGAGCCATC CGAGCAGTTG CCCCAAATGT GATCCAAGCT GTCCCAATGG AAGCTGCTGG 600

GGAGGAGGAG AGGAGAACTG CCAGAAATTG ACCAAAATCA TCTGTGCCCA GCAATGTTCC 660

CATCGCTGTC GTGGCAGGTC CCCCAGTGAC TGCTGCCACA ACCAATGTGC TGCGGGGTGT 720

ACAGGGCCCC GAGAGAGTGA CTGTCTGGTC TGCCAAAAGT TCCAAGATGA GGCCACATGC 780

AAAGACACCT GCCCACCACT CATGCTGTAC AACCCCACCA CCTATCAGAT GGATGTCAAC 840

CCTGAAGGGA AGTACAGCTT TGGTGCCACC TGTGTGAAGA AGTGCCCCCG AAACTACGTG 900

GTGACAGATC ATGGCTCATG TGTCCGAGCC TGTGGGCCTG ACTACTACGA AGTGGAAGAA 960

GATGGCATCC GCAAGTGTAA AAAATGTGAT GGGCCCTGTC GCAAAGTTTG TAATGGCATA 1020

GGCATTGGTG AATTTAAAGA CACACTCTCC ATAAATGCTA CAAACATCAA ACACTTCAAA 1080

TACTGCACTG CCATCAGCGG GGACCTTCAC ATCCTGCCAG TGGCCTTTAA GGGGGATTCT 1140

TTCACGCGCA CTCCTCCTCT AGACCCACGA GAACTAGAAA TTCTAAAAAC CGTAAAGGAA 1200

ATAACAGGCT TTTTGCTGAT TCAGGCTTGG CCTGATAACT GGACTGACCT CCATGCTTTC 1260

GAGAACCTAG AAATAATACG TGGCAGAACA AAGCAACATG GTCAGTTTTC TTTGGCGGTC 1320

GTTGGCCTGA ACATCACATC ACTGGGGCTG CGTTCCCTCA AGGAGATCAG TGATGGGGAT 1380

GTGATCATTT CTGGAAACCG AAATTTGTGC TACGCAAACA CAATAAACTG GAAAAAACTC 1440

TTCGGGACAC CCAATCAGAA AACCAAAATC ATGAACAACA GAGCTGAGAA AGACTGCAAG 1500

GCCGTGAACC ACGTCTGCAA TCCTTTATGC TCCTCGGAAG GCTGCTGGGG CCCTGAGCCC 1560

AGGGACTGTG TCTCCTGCCA GAATGTGAGC AGAGGCAGGG AGTGCGTGGA GAAATGCAAC 1620

ATCCTGGAGG GGGAACCAAG GGAGTTTGTG GAAAATTCTG AATGCATCCA GTGCCATCCA 1680

GAATGTCTGC CCCAGGCCAT GAACATCACC TGTACAGGCA GGGGACCAGA CAACTGCATC 1740

CAGTGTGCCC ACTACATTGA TGGCCCACAC TGTGTCAAGA CCTGCCCAGC TGGCATCATG 1800

GGAGAGAACA ACACTCTGGT CTGGAAGTAT GCAGATGCCA ATAATGTCTG CCACCTATGC 1860

CACGCCAACT GTACCTATGG ATGTGCTGGG CCAGGTCTTC AAGGATGTGA AGTGTGGCCA 1920

TCTGGGCCAA AGATACCATC TATTGCCACT GGGATTGTGG GTGGCCTCCT CTTCATAGTG 1980

GTGGTGGCCC TTGGGATTGG CCTATTCATG CGAAGACGTC ACATTGTTCG AAAGCGTACA 2040

CTACGCCGCC TGCTTCAAGA GAGAGAGCTC GTGGAACCTC TCACACCCAG CGGAGAAGCT 2100

CCAAACCAAG CCCACTTGAG GATATTAAAG GAAACAGAAT TCAAAAAGAT CAAAGTTCTG 2160

GGTTCGGGAG CATTTGGCAC AGTGTATAAG GGTCTCTGGA TCCCAGAAGG TGAGAAAGTA 2220

AAAATCCCGG TGGCCATCAA GGAGTTAAGA GAAGCCACAT CTCCAAAAGC CAACAAAGAA 2280

ATCCTTGACG AAGCCTATGT GATGGCTAGT GTGGACAACC CTCATGTATG CCGCCTCCTG 2340

GGCATCTGTC TGACCTCCAC TGTCCAGCTC ATTACACAGC TCATGCCCTA CGGTTGCCTC 2400

CTGGACTACG TCCGAGAACA CAAGGACAAC ATTGGCTCCC AGTACCTCCT CAACTGGTGT 2460

GTGCAGATTG CAAAGGGCAT GAACTACCTG GAAGATCGGC GTTTGGTGCA CCGTGACTTG 2520

GCAGCCAGGA ATGTACTGGT GAAGACACCA CAGCATGTCA AGATCACAGA TTTTGGGCTG 2580

GCCAAACTGC TTGGTGCTGA AGAGAAAGAA TATCATGCCG AGGGGGGCAA AGTGCCTATC 2640

AAGTGGATGG CTTTGGAATC AATTTTACAC CGAATTTATA CACACCAAAG TGATGTCTGG 2700

AGCTATGGTG TCACTGTGTG GGAACTGATG ACCTTTGGGT CCAAGCCTTA TGATGGAATC 2760

CCAGCAAGTG ACATCTCATC CATCCTAGAG AAAGGAGAGC GCCTTCCACA GCCACCTATC 2820

TGCACCATCG ATGTCTACAT GATCATGGTC AAGTGCTGGA TGATAGATGC TGATAGCCGC 2880

CCAAAGTTCC GAGAGTTGAT TCTTGAATTC TCCAAAATGG CCCGAGACCC ACAGCGCTAC 2940

CTTGTTATCC AGGGGGATGA AAGAATGCAT TTGCCAAGCC CTACAGACTC CAACTTTTAC 3000

CGAGCCCTGA TGGATGAAGA GGACATGGAG GATGTAGTTG ATGCTGATGA GTATCTTATC 3060

CCACAGCAAG GCTTCTTCAA CAGCCCGTCC ACGTCGAGGA CTCCCCTCTT GAGTTCTCTG 3120

AGTGCAACTA GCAACAATTC CACTGTGGCT TGCATTAATA GAAATGGGAG CTGCCGTGTC 3180

AAAGAAGACG CCTTCTTGCA GCGGTACAGC TCCGACCCCA CAGGTGCTGT AACAGAGGAC 3240

AACATAGATG ACGCATTCCT CCCTGTACCT GAATATGTAA ACCAATCTGT TCCCAAGAGG 3300

CCAGCAGGCT CTGTGCAGAA CCCTGTCTAT CACAATCAGC CCCTGCATCC AGCTCCTGGA 3360

AGAGACCTGC ATTATCAAAA TCCCCACAGC AATGCAGTGG GCAACCCTGA GTATCTCAAC 3420

ACTGCCCAGC CTACCTGTCT CAGTAGTGGG TTTAACAGCC CTGCACTCTG GATCCAGAAA 3480

GGCAGTCACC AAATGAGCCT AGACAACCCT GACTACCAGC AGGACTTCTT CCCCAAGGAA 3540

ACCAAGCCAA ATGGCATATT TAAGGGCCCC ACAGCTGAAA ATGCAGAGTA CCTACGGGTG 3600

GCACCTCCAA GCAGTGAGTT TATTGGAGCA TGA 3633

Domain Profile

S: 1     lkklkvlGsGafGtvykGiwipegenvkipvaikvleektgkkankelldealvlasldh  60

         +kk+kvlGsGafGtvykG+wipege+vkipvaik+l+e+t++kanke+ldea+v+as+d+

Q: 714   FKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDN  773

         59**********************************************************

S: 61    anlvrllglclsstlqlvtqllplGclldyvrehkdklGsqellnwcvqiakGmsyleek  120

         ++++rllg+cl+st+ql+tql+p+Gclldyvrehkd++Gsq+llnwcvqiakGm+yle++

Q: 774   PHVCRLLGICLTSTVQLITQLMPYGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDR  833

         ************************************************************

S: 121   rlvhrdlaarnvlvkspnhvkitdfGlaklleadekelkaeggkvpikwlalesilkrky  180

         rlvhrdlaarnvlvk+p+hvkitdfGlakll a+eke++aeggkvpikw+alesil+r+y

Q: 834   RLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIY  893

         ************************************************************

S: 181   thqsdvwsyGvtvwelltfGakpydgipakeipdllekGerlaqppictidvymvlvkcw  240

         thqsdvwsyGvtvwel+tfG+kpydgipa++i+++lekGerl+qppictidvym++vkcw

Q: 894   THQSDVWSYGVTVWELMTFGSKPYDGIPASDISSILEKGERLPQPPICTIDVYMIMVKCW  953

         ************************************************************

S: 241   midaesrpkfkelaeefs  258

         mida+srpkf+el+ efs

Q: 954   MIDADSRPKFRELILEFS  971

         ***************996

Domain Sequence
(FASTA)

FKKIKVLGSG AFGTVYKGLW IPEGEKVKIP VAIKELREAT SPKANKEILD EAYVMASVDN 60

PHVCRLLGIC LTSTVQLITQ LMPYGCLLDY VREHKDNIGS QYLLNWCVQI AKGMNYLEDR 120

RLVHRDLAAR NVLVKTPQHV KITDFGLAKL LGAEEKEYHA EGGKVPIKWM ALESILHRIY 180

THQSDVWSYG VTVWELMTFG SKPYDGIPAS DISSILEKGE RLPQPPICTI DVYMIMVKCW 240

MIDADSRPKF RELILEFS 258

Keyword

ATP-binding; Cell membrane; Complete proteome; Developmental protein; Disulfide bond; Endoplasmic reticulum; Endosome; Glycoprotein; Golgi apparatus; Isopeptide bond; Kinase; Membrane; Methylation; Nucleotide-binding; Nucleus; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016323	; C:basolateral plasma membrane
GO:0030139	; C:endocytic vesicle
GO:0005789	; C:endoplasmic reticulum membrane
GO:0005768	; C:endosome
GO:0010008	; C:endosome membrane
GO:0000139	; C:Golgi membrane
GO:0016021	; C:integral to membrane
GO:0045121	; C:membrane raft
GO:0031965	; C:nuclear membrane
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0005006	; F:epidermal growth factor-activated receptor activity
GO:0030235	; F:nitric-oxide synthase regulator activity
GO:0004716	; F:receptor signaling protein tyrosine kinase activity
GO:0000902	; P:cell morphogenesis
GO:0008283	; P:cell proliferation
GO:0016337	; P:cell-cell adhesion
GO:0021795	; P:cerebral cortex cell migration
GO:0048546	; P:digestive tract morphogenesis
GO:0001892	; P:embryonic placenta development
GO:0001942	; P:hair follicle development
GO:0060571	; P:morphogenesis of an epithelial fold
GO:0043066	; P:negative regulation of apoptotic process
GO:0042177	; P:negative regulation of protein catabolic process
GO:0035413	; P:positive regulation of catenin import into nucleus
GO:0030335	; P:positive regulation of cell migration
GO:0031659	; P:positive regulation of cyclin-dependent protein kinase activity involved in G1/S
GO:0045739	; P:positive regulation of DNA repair
GO:0045740	; P:positive regulation of DNA replication
GO:0050679	; P:positive regulation of epithelial cell proliferation
GO:0048146	; P:positive regulation of fibroblast proliferation
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0045429	; P:positive regulation of nitric oxide biosynthetic process
GO:0051897	; P:positive regulation of protein kinase B signaling cascade
GO:0046777	; P:protein autophosphorylation
GO:0050999	; P:regulation of nitric-oxide synthase activity
GO:0050730	; P:regulation of peptidyl-tyrosine phosphorylation
GO:0070141	; P:response to UV-A
GO:0007435	; P:salivary gland morphogenesis
GO:0023014	; P:signal transduction by phosphorylation

KEGG

mmu:13649

;

InterPros

IPR000494	; EGF_rcpt_L.
IPR006211	; Furin-like_Cys-rich_dom.
IPR006212	; Furin_repeat.
IPR009030	; Growth_fac_rcpt.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016245	; Tyr_kinase_EGF/ERB/XmrK_rcpt.

Pfam

PF00757	; Furin-like; 1.
PF07714	; Pkinase_Tyr; 1.
PF01030	; Recep_L_domain; 2.

SMARTs

SM00261	; FU; 4.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013