EPS-MUM-00020

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00020

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	268.1	2.3E-79	63	366	304

Status

Reviewed

Ensembl Protein

ENSMUSP00000020835

UniProt Accession

Q9QZ67; B1B0B0;

Protein Name

Protein phosphatase 1D

Protein Synonyms/Alias

Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1;

Gene Name

Ppm1d

Gene Synonyms/Alias

Ppm1d; Wip1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000020525	ENSMUSP00000020835	ENSMUST00000020835
ENSMUSG00000020525	ENSMUSP00000115606	ENSMUST00000127717

Organism

Mus musculus

Functional Description

Required for the relief of p53-dependent checkpointmediated cell cycle arrest. Binds to and dephosphorylates 'Ser-18' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (By similarity).

Protein Length

598

Protein Sequence
(FASTA)

MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPAAEDKPA PVPRRALGLP ATPTLAGVGP 60

SEKGPAAARD PAPDAAASLP AGRCCRRRSS VAFFAVCDGH GGREAAQFAR EHLWGFIKKQ 120

KGFTSSEPAK VCAAIRKGFL ACHLAMWKKL AEWPKTMTGL PSTSGTTASV VIIRGMKMYV 180

AHVGDSGVVL GIQDDPKDDF VRAVEVTQDH KPELPKERER IEGLGGSVMN KSGVNRVVWK 240

RPRLTHSGPV RRSTVIDQIP FLAVARALGD LWSYDFFSGK FVVSPEPDTS VHTLDPRKHK 300

YIILGSDGLW NMVPPQDAIS MCQDQEEKKY LMGEQGQSCA KMLVNRALGR WRQRMLRADN 360

TSAIVICISP EVDNQGNFTN EDELFLNLTD SPTYNSQETC VMTSSPSSTP PIKSPEEDAW 420

PRLSSKDHIP ALVRSNAFSE KFLEVPAEIA RGNIQTVVMT SKDSETLEEN CPKALTLRIH 480

DSLNNTLSVG LIPTNSTNTI MDQKNLKMST PGQMKAQEVE RTPPANFKRT LEESNSGPLM 540

KKHRRNGLSR SSGAQASSLP TASQRRHSVK LTLRRRLRGQ RKMGNPLLHQ HRKTVCVC 598

Nucleotide Sequence
(FASTA)

ATGGCGGGGC TGTACTCGCT GGGAGTGAGC GTCTTCTCGG ACCAGGGCGG GAGGAAGTAC 60

ATGGAGGACG TAACTCAGAT CGTGGTGGAG CCCGAGCCGG CTGCGGAGGA CAAGCCGGCG 120

CCGGTACCGC GGCGGGCGCT CGGGTTGCCG GCGACCCCCA CTCTCGCCGG CGTCGGGCCA 180

TCGGAAAAAG GCCCGGCGGC GGCCCGCGAC CCTGCCCCGG ACGCCGCGGC CTCGCTACCC 240

GCTGGCCGCT GCTGTCGCCG CCGCTCTTCG GTGGCCTTCT TTGCAGTGTG CGACGGGCAC 300

GGCGGTCGGG AGGCGGCACA GTTTGCCCGG GAGCACTTGT GGGGTTTCAT CAAGAAGCAG 360

AAAGGCTTCA CCTCGTCCGA GCCCGCCAAG GTGTGCGCTG CCATCCGCAA AGGTTTCCTC 420

GCCTGTCACC TCGCCATGTG GAAGAAACTG GCAGAATGGC CAAAGACTAT GACAGGTCTT 480

CCCAGCACGT CCGGGACAAC TGCCAGTGTG GTTATAATAC GGGGCATGAA GATGTATGTA 540

GCGCATGTAG GTGACTCTGG GGTGGTCCTT GGAATTCAGG ATGACCCAAA GGATGATTTT 600

GTGAGAGCTG TGGAGGTGAC ACAAGATCAC AAGCCAGAAC TTCCCAAGGA AAGAGAAAGA 660

ATCGAAGGAC TTGGCGGCAG TGTGATGAAC AAGTCTGGAG TGAACCGAGT AGTTTGGAAA 720

AGGCCCCGGC TCACTCACAG TGGACCTGTC AGAAGGAGCA CAGTCATTGA CCAGATTCCT 780

TTTCTGGCTG TAGCAAGAGC GCTTGGTGAC TTGTGGAGCT ATGATTTCTT CAGTGGTAAG 840

TTTGTGGTGT CACCTGAACC AGACACCAGT GTCCACACTC TTGACCCCCG GAAGCACAAG 900

TATATTATCC TGGGAAGTGA TGGACTTTGG AATATGGTTC CACCTCAAGA TGCCATCTCC 960

ATGTGCCAAG ACCAAGAGGA GAAAAAATAC TTGATGGGTG AGCAAGGACA GTCCTGTGCC 1020

AAAATGCTTG TGAATCGAGC ACTTGGCCGC TGGAGGCAGC GTATGCTTCG GGCAGATAAC 1080

ACAAGTGCCA TCGTAATCTG CATCTCTCCA GAAGTAGACA ACCAAGGGAA CTTCACCAAT 1140

GAAGATGAGC TCTTTCTGAA CCTGACTGAT AGCCCTACTT ACAACAGCCA GGAGACCTGT 1200

GTGATGACTT CTTCTCCAAG TTCTACACCA CCAATCAAGT CACCGGAAGA AGATGCATGG 1260

CCAAGGCTGA GCTCTAAGGA CCATATACCT GCCCTTGTTC GCAGTAATGC CTTCTCAGAG 1320

AAGTTTTTAG AGGTCCCAGC TGAGATAGCT AGAGGGAATA TCCAGACTGT AGTGATGACC 1380

TCAAAAGACT CAGAGACACT TGAAGAAAAT TGCCCCAAAG CCCTGACTTT AAGGATTCAT 1440

GATTCTTTGA ATAATACTCT GTCAGTTGGC CTCATTCCAA CCAATTCAAC AAATACTATC 1500

ATGGACCAAA AAAACTTAAA GATGTCAACT CCAGGTCAAA TGAAAGCTCA AGAAGTTGAA 1560

AGAACCCCTC CAGCCAATTT TAAAAGGACA TTAGAAGAAT CCAACTCTGG CCCCCTTATG 1620

AAGAAGCACC GACGAAATGG CTTAAGTCGA AGTAGCGGGG CCCAGGCTTC CAGTCTCCCT 1680

ACAGCATCCC AGCGCAGGCA CTCTGTCAAA CTGACCCTGA GGCGCAGACT CAGGGGCCAG 1740

AGGAAGATGG GAAATCCTCT TCTCCACCAG CACCGGAAAA CAGTGTGTGT GTGCTGA 1797

Domain Profile

S: 13    nemedavtsspnflkspekkdr......laffgvyDGhggsevakfcseklheilaeele  66

         +    a++++p++++s  + ++      +aff+v+DGhgg+e+a+f++e+l+ ++++++

Q: 63    KGPAAARDPAPDAAASLPAGRCcrrrssVAFFAVCDGHGGREAAQFAREHLWGFIKKQKG  122

         5556677788888888888877888888********************************

S: 67    sseseerkvkkalakaFlstdeel.....kvleeksldidksGstavvalikdsklyvan  121

         +++se +kv++a++k+Fl+++ ++     +++++++  +++sG+ta+v++i+++k+yva+

Q: 123   FTSSEPAKVCAAIRKGFLACHLAMwkklaEWPKTMTGLPSTSGTTASVVIIRGMKMYVAH  182

         ************************************************************

S: 122   vGdSravLcrngk.......avelssdHkpsredEleRieeaggsvilkdga.Rv.....  168

         vGdS++vL+ +++       ave+++dHkp+ ++E+eRie  ggsv++k+g+ Rv

Q: 183   VGDSGVVLGIQDDpkddfvrAVEVTQDHKPELPKERERIEGLGGSVMNKSGVnRVvwkrp  242

         **********77656999999***************************************

S: 169   ................lGvLavsRalGdlylk......pyvtpePevtshrltsede.fl  205

                         +++Lav+RalGdl+++      ++v+peP++++h+l+++++ ++

Q: 243   rlthsgpvrrstvidqIPFLAVARALGDLWSYdffsgkFVVSPEPDTSVHTLDPRKHkYI  302

         *************************************9**********************

S: 206   iLasDGlwdvvsneeavslvrdvle....kkeaeqeaakkLvklalargsk.....dnvs  256

         iL+sDGlw++v++++a+s+++d++e    ++e+ q++ak+Lv++al r+++     dn+s

Q: 303   ILGSDGLWNMVPPQDAISMCQDQEEkkylMGEQGQSCAKMLVNRALGRWRQrmlraDNTS  362

         ************************************************************

S: 257   aivv  260

         aiv+

Q: 363   AIVI  366

         **97

Domain Sequence
(FASTA)

KGPAAARDPA PDAAASLPAG RCCRRRSSVA FFAVCDGHGG REAAQFAREH LWGFIKKQKG 60

FTSSEPAKVC AAIRKGFLAC HLAMWKKLAE WPKTMTGLPS TSGTTASVVI IRGMKMYVAH 120

VGDSGVVLGI QDDPKDDFVR AVEVTQDHKP ELPKERERIE GLGGSVMNKS GVNRVVWKRP 180

RLTHSGPVRR STVIDQIPFL AVARALGDLW SYDFFSGKFV VSPEPDTSVH TLDPRKHKYI 240

ILGSDGLWNM VPPQDAISMC QDQEEKKYLM GEQGQSCAKM LVNRALGRWR QRMLRADNTS 300

AIVI 304

Keyword

Cell cycle; Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Phosphoprotein; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0004724	; F:magnesium-dependent protein serine/threonine phosphatase activity
GO:0046872	; F:metal ion binding
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0009617	; P:response to bacterium

KEGG

mmu:53892

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013