EKS-MUM-00163

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00163

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/TLK	623.7	8.4E-188	408	687	280

Status

Reviewed

Ensembl Protein

ENSMUSP00000015107

UniProt Accession

O55047; B1ASU7; B1ASU8; Q9D5Y5;

Protein Name

Serine/threonine-protein kinase tousled-like 2

Protein Synonyms/Alias

PKU-alpha; Tousled-like kinase 2;

Gene Name

Tlk2

Gene Synonyms/Alias

Tlk2; Tlk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000020694	ENSMUSP00000090198	ENSMUST00000092537
ENSMUSG00000020694	ENSMUSP00000102554	ENSMUST00000106941
ENSMUSG00000020694	ENSMUSP00000118520	ENSMUST00000145048
ENSMUSG00000020694	ENSMUSP00000121897	ENSMUST00000149459
ENSMUSG00000020694	ENSMUSP00000102552	ENSMUST00000106939
ENSMUSG00000020694	ENSMUSP00000120944	ENSMUST00000126175
ENSMUSG00000020694	ENSMUSP00000123179	ENSMUST00000152404
ENSMUSG00000020694	ENSMUSP00000015107	ENSMUST00000015107

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase involved in the processof chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (By similarity). Negative regulator of amino acid starvation-induced autophagy (By similarity). Testis-specific isoforms may play a role inspermatogenesis. Highly expressed in embryos throughout development.

Protein Length

718

Protein Sequence
(FASTA)

MMEELHSLDP RRQELLEARF TGVGVSKGPL NSESSNQSLC SVGSLSDKEV ETPEKKQNDQ 60

RNRKRKAEPY DTSQGKGTPR GHKISDYFER RAEQPLYGLD GSAAKEASEE QSALPTLMSV 120

MLAKPRLDTE QLAPRGAGLC FTFVSAQQNS PSSTGSGNTE HSCSSQKQIS IQHRQTQSDL 180

TIEKISALEN SKNSDLEKKE GRIDDLLRAN CDLRRQIDEQ QKMLEKYKER LNRCVTMSKK 240

LLIEKSKQEK MACRDKSMQD RLRLGHFTTV RHGASFTEQW TDGYAFQNLI KQQERINSQR 300

EEIERQRKML AKRKPPAMGQ APPATNEQKQ RKSKTNGAEN ETLTLAEYHE QEEIFKLRLG 360

HLKKEEAEIQ AELERLERVR NLHIRELKRI HNEDNSQFKD HPTLNDRYLL LHLLGRGGFS 420

EVYKAFDLTE QRYVAVKIHQ LNKNWRDEKK ENYHKHACRE YRIHKELDHP RIVKLYDYFS 480

LDTDSFCTVL EYCEGNDLDF YLKQHKLMSE KEARSIIMQI VNALKYLNEI KPPIIHYDLK 540

PGNILLVNGT ACGEIKITDF GLSKIMDDDS YNSVDGMELT SQGAGTYWYL PPECFVVGKE 600

PPKISNKVDV WSVGVIFYQC LYGRKPFGHN QSQQDILQEN TILKATEVQF PPKPVVTPEA 660

KAFIRRCLAY RKEDRIDVQQ LACDPYLLPH IRKSVSTSSP AGAAIASTSG ASNNSSSN 718

Nucleotide Sequence
(FASTA)

ATGATGGAAG AACTGCATAG CCTGGACCCA CGAAGGCAGG AATTACTAGA GGCCAGGTTC 60

ACTGGAGTTG GTGTAAGTAA GGGGCCACTC AACAGTGAGT CTTCCAACCA GAGTCTGTGC 120

AGCGTGGGGT CGTTGAGTGA TAAAGAAGTA GAGACTCCTG AGAAAAAGCA GAATGACCAG 180

CGAAATCGGA AAAGGAAAGC CGAGCCATAT GACACTAGCC AAGGGAAAGG CACTCCTAGG 240

GGACATAAAA TTAGTGATTA CTTTGAGCGT CGAGCAGAAC AGCCTCTGTA TGGTTTAGAT 300

GGCAGTGCAG CAAAGGAGGC CTCAGAAGAG CAGTCTGCTC TGCCCACCCT CATGTCAGTG 360

ATGTTAGCAA AACCGCGACT TGACACAGAG CAGTTAGCGC CACGGGGAGC TGGCCTCTGC 420

TTCACTTTCG TCTCTGCTCA ACAAAACAGT CCTTCTTCTA CGGGTTCTGG CAATACAGAA 480

CATTCTTGCA GCTCCCAAAA ACAGATCTCC ATCCAGCACA GACAGACCCA GTCTGACCTC 540

ACAATAGAAA AAATATCTGC ACTAGAAAAC AGTAAGAACT CTGACTTAGA GAAGAAGGAA 600

GGAAGAATAG ATGATTTATT AAGAGCCAAC TGTGATTTGA GACGACAGAT AGATGAACAG 660

CAAAAGATGC TAGAGAAATA CAAGGAACGA TTAAATAGAT GTGTCACCAT GAGCAAGAAG 720

CTCCTTATAG AAAAGTCAAA ACAAGAGAAG ATGGCGTGCA GAGATAAGAG CATGCAGGAC 780

CGATTGCGAT TAGGCCACTT TACTACTGTC CGGCATGGAG CCTCGTTTAC TGAGCAGTGG 840

ACAGATGGTT ATGCTTTCCA AAACCTCATC AAGCAACAGG AAAGGATAAA TTCACAGAGA 900

GAAGAGATAG AAAGGCAACG GAAAATGTTA GCAAAACGGA AACCTCCTGC CATGGGTCAG 960

GCCCCTCCTG CAACCAATGA GCAGAAACAA CGGAAAAGCA AGACTAATGG AGCTGAAAAT 1020

GAAACGTTAA CGTTAGCCGA GTACCATGAA CAAGAGGAAA TCTTCAAACT TAGATTAGGT 1080

CATCTTAAGA AAGAGGAAGC AGAAATCCAG GCAGAGCTGG AAAGGCTGGA AAGGGTTAGG 1140

AATCTACACA TCAGGGAATT AAAAAGGATA CATAATGAAG ACAATTCGCA GTTTAAAGAC 1200

CATCCAACAC TAAATGACAG ATATTTGTTG TTACATCTTT TGGGTAGAGG AGGTTTCAGT 1260

GAAGTTTACA AGGCATTTGA TCTAACGGAG CAAAGATATG TAGCTGTGAA AATTCACCAG 1320

TTAAATAAAA ACTGGAGAGA TGAGAAAAAG GAGAATTACC ACAAGCATGC GTGTAGGGAA 1380

TACCGGATTC ACAAGGAGCT GGACCACCCC AGGATAGTGA AGCTGTATGA TTACTTTTCA 1440

CTGGACACTG ACTCGTTTTG TACAGTATTA GAATACTGTG AAGGGAATGA CCTGGACTTC 1500

TACCTAAAAC AGCACAAATT AATGTCGGAG AAAGAAGCCC GATCCATTAT TATGCAGATT 1560

GTGAATGCTT TAAAGTACTT AAATGAAATA AAACCTCCCA TCATACACTA TGACCTCAAA 1620

CCAGGTAATA TCCTTTTAGT AAATGGTACA GCATGTGGAG AGATAAAAAT TACAGATTTT 1680

GGTCTTTCCA AGATCATGGA TGATGATAGC TACAATTCAG TGGATGGCAT GGAGCTGACG 1740

TCACAAGGAG CTGGTACTTA TTGGTATTTA CCACCAGAGT GTTTTGTGGT TGGGAAAGAG 1800

CCACCAAAGA TCTCAAATAA AGTCGATGTT TGGTCAGTGG GTGTGATCTT CTACCAGTGT 1860

CTTTATGGGA GGAAGCCTTT TGGCCATAAC CAGTCCCAGC AAGATATTCT ACAAGAGAAT 1920

ACTATTCTTA AGGCTACTGA AGTACAGTTC CCGCCAAAGC CAGTAGTAAC ACCTGAAGCA 1980

AAGGCATTTA TCAGGAGATG TCTGGCCTAT CGAAAGGAAG ATCGCATTGA TGTGCAGCAG 2040

CTGGCCTGTG ACCCCTACTT GTTGCCTCAC ATCCGAAAGT CAGTCTCCAC AAGTAGCCCT 2100

GCAGGAGCTG CTATTGCATC AACCTCTGGG GCATCCAATA ACAGTTCTTC GAATTGA 2157

Domain Profile

S: 1     ylllkllgkggfsevykafdlkeqryvavkihqlnkdwkdekkenyvkhalreyrihkel  60

         ylll+llg+ggfsevykafdl+eqryvavkihqlnk+w+dekkeny+kha+reyrihkel

Q: 408   YLLLHLLGRGGFSEVYKAFDLTEQRYVAVKIHQLNKNWRDEKKENYHKHACREYRIHKEL  467

         9***********************************************************

S: 61    dhprivklydllsldtdsfctvleyvegndldfylkqhkllsekearsiimqivsalkyl  120

         dhprivklyd++sldtdsfctvley+egndldfylkqhkl+sekearsiimqiv+alkyl

Q: 468   DHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIIMQIVNALKYL  527

         ************************************************************

S: 121   neikppiihydlkpgnillvegtacgeikitdfglskimdddsydsddgleltsqgagty  180

         neikppiihydlkpgnillv+gtacgeikitdfglskimdddsy+s+dg+eltsqgagty

Q: 528   NEIKPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTY  587

         ************************************************************

S: 181   wylppecfvvgkeppkisnkvdvwsvgvifyqclygkkpfghnqsqqdileentilkate  240

         wylppecfvvgkeppkisnkvdvwsvgvifyqclyg+kpfghnqsqqdil+entilkate

Q: 588   WYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATE  647

         ************************************************************

S: 241   vqfpskpvvsseakafirrclayrkedridvqqlakdeyl  280

         vqfp+kpvv++eakafirrclayrkedridvqqla+d+yl

Q: 648   VQFPPKPVVTPEAKAFIRRCLAYRKEDRIDVQQLACDPYL  687

         **************************************97

Domain Sequence
(FASTA)

YLLLHLLGRG GFSEVYKAFD LTEQRYVAVK IHQLNKNWRD EKKENYHKHA CREYRIHKEL 60

DHPRIVKLYD YFSLDTDSFC TVLEYCEGND LDFYLKQHKL MSEKEARSII MQIVNALKYL 120

NEIKPPIIHY DLKPGNILLV NGTACGEIKI TDFGLSKIMD DDSYNSVDGM ELTSQGAGTY 180

WYLPPECFVV GKEPPKISNK VDVWSVGVIF YQCLYGRKPF GHNQSQQDIL QENTILKATE 240

VQFPPKPVVT PEAKAFIRRC LAYRKEDRID VQQLACDPYL 280

Keyword

Alternative splicing; ATP-binding; Cell cycle; Chromatin regulator; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Spermatogenesis; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005882	; C:intermediate filament
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0030154	; P:cell differentiation
GO:0016568	; P:chromatin modification
GO:0007059	; P:chromosome segregation
GO:0035556	; P:intracellular signal transduction
GO:0007275	; P:multicellular organismal development
GO:0032435	; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0001672	; P:regulation of chromatin assembly or disassembly
GO:0006974	; P:response to DNA damage stimulus
GO:0007283	; P:spermatogenesis

KEGG

mmu:24086

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013