EKS-MUM-00418

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00418

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	368.9	4.3E-110	362	621	260

Status

Reviewed

Ensembl Protein

ENSMUSP00000002044

UniProt Accession

Q61084;

Protein Name

Mitogen-activated protein kinase kinase kinase 3

Protein Synonyms/Alias

MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3;

Gene Name

Map3k3

Gene Synonyms/Alias

Map3k3; Mekk3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000020700	ENSMUSP00000002044	ENSMUST00000002044

Organism

Mus musculus

Functional Description

Component of a protein kinase signal transductioncascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

Protein Length

626

Protein Sequence
(FASTA)

MDEQEALDSI MKDLVALQMS RRTRLSGYET MKNKDTGHPN RQSDVRIKFE HNGERRIIAF 60

SRPVRYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL DKAIDILDRS SSMKSLRILL 120

LSQDRNHTSS SPHSGVSRQV RIKPSQSAGD INTIYQAPEP RSRHLSVSSQ NPGRSSPPPG 180

YVPERQQHIA RQGSYTSINS EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF 240

RKSQMSRARS FPDNRKECSD RETQLYDKGV KGGTYPRRYH VSVHHKDYND GRRTFPRIRR 300

HQGNLFTLVP SSRSLSTNGE NMGVAVQYLD PRGRLRSADS ENALTVQERN VPTKSPSAPI 360

NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP ETSKEVSALE CEIQLLKNLQ 420

HERIVQYYGC LRDRAEKILT IFMEYMPGGS VKDQLKAYGA LTESVTRKYT RQILEGMSYL 480

HSNMIVHRDI KGANILRDSA GNVKLGDFGA SKRLQTICMS GTGIRSVTGT PYWMSPEVIS 540

GEGYGRKADV WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL 600

RRIFVEARQR PSAEELLTHH FAQLVY 626

Nucleotide Sequence
(FASTA)

ATGGATGAAC AAGAGGCATT AGACTCGATC ATGAAGGACC TGGTGGCCCT CCAGATGAGC 60

CGACGAACCC GGTTGTCTGG ATATGAGACC ATGAAGAATA AGGACACAGG TCACCCAAAC 120

AGGCAGAGTG ACGTCAGAAT CAAGTTTGAA CACAATGGGG AGAGACGAAT TATAGCATTC 180

AGCCGGCCTG TGAGATACGA AGATGTGGAG CACAAGGTGA CAACAGTCTT TGGGCAGCCT 240

CTTGATTTGC ATTATATGAA TAATGAGCTC TCCATCCTGT TGAAAAACCA AGATGATCTC 300

GATAAAGCCA TTGACATTTT GGATAGAAGC TCAAGTATGA AAAGCCTTAG GATACTACTG 360

TTATCCCAAG ACAGAAACCA TACTAGTTCC TCTCCCCACT CTGGAGTGTC CAGGCAGGTT 420

CGGATCAAGC CTTCCCAGTC TGCAGGGGAT ATAAATACCA TCTACCAAGC TCCTGAGCCC 480

AGAAGCAGGC ACCTGTCTGT CAGCTCCCAG AACCCTGGCC GAAGCTCTCC TCCCCCGGGA 540

TATGTACCTG AGCGACAACA GCACATTGCC CGGCAAGGAT CCTATACGAG CATCAACAGC 600

GAAGGTGAAT TCATCCCAGA GACCAGCGAA CAGTGTATGC TAGATCCCCT CAGCAGTGCC 660

GAAAATTCCT TGTCAGGAAG CTGCCAATCC TTGGACAGGT CAGCAGACAG CCCATCCTTC 720

AGGAAATCAC AAATGTCCCG AGCCCGGAGC TTCCCAGACA ACAGAAAGGA ATGCTCAGAT 780

CGGGAGACCC AGCTCTATGA TAAAGGTGTC AAAGGTGGAA CCTATCCCAG GCGCTACCAT 840

GTGTCTGTGC ATCACAAAGA CTACAATGAT GGCAGAAGAA CATTTCCCCG AATACGACGG 900

CATCAAGGCA ACCTATTCAC TCTGGTGCCC TCAAGTCGCT CCTTGAGCAC AAATGGCGAG 960

AACATGGGTG TAGCTGTGCA ATACCTGGAC CCCCGTGGGC GCCTACGGAG TGCAGACAGT 1020

GAGAATGCCC TCACTGTGCA GGAAAGGAAT GTGCCAACCA AATCTCCTAG TGCTCCCATC 1080

AATTGGCGTC GGGGGAAGCT CCTGGGTCAA GGTGCCTTCG GCAGGGTCTA CTTGTGCTAT 1140

GATGTGGACA CAGGACGTGA ACTTGCTTCT AAGCAGGTCC AGTTTGACCC AGATAGTCCT 1200

GAGACAAGCA AGGAGGTGAG TGCTCTGGAG TGTGAGATCC AGTTGCTGAA GAACCTGCAG 1260

CATGAGCGCA TTGTGCAGTA CTACGGCTGC CTGCGGGACC GTGCTGAGAA GATCCTCACC 1320

ATCTTTATGG AGTATATGCC AGGGGGCTCT GTAAAAGACC AGTTGAAGGC CTACGGAGCT 1380

CTGACAGAGA GTGTGACCCG CAAGTACACC CGGCAGATTC TGGAGGGCAT GTCATACCTG 1440

CACAGCAACA TGATTGTGCA TCGGGACATC AAGGGAGCCA ATATCCTCCG AGACTCAGCT 1500

GGGAATGTGA AGCTTGGGGA TTTTGGGGCC AGCAAACGCC TACAGACCAT CTGCATGTCA 1560

GGGACAGGCA TTCGCTCTGT CACTGGCACA CCCTACTGGA TGAGTCCTGA AGTCATCAGT 1620

GGCGAGGGCT ATGGAAGAAA GGCAGACGTG TGGAGCCTGG GCTGTACTGT GGTGGAAATG 1680

CTGACAGAGA AACCACCTTG GGCAGAGTAT GAAGCTATGG CTGCCATTTT CAAGATTGCC 1740

ACCCAGCCTA CCAATCCTCA GCTGCCCTCT CACATCTCAG AACACGGCAG GGACTTCCTG 1800

AGGCGCATAT TTGTGGAAGC TCGTCAGAGA CCCTCAGCTG AGGAGCTGCT CACACACCAC 1860

TTTGCACAGC TAGTGTACTG A 1881

Domain Profile

S: 1     wqkrevigkGsfgsvyaaldvdtgellavKeveikpekksekkevkalkeEikllkelkH  60

         w++++++g+G+fg+vy+++dvdtg+ la K+v+++p++ +++kev+al++Ei+llk+l+H

Q: 362   WRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQH  421

         899*********************************************************

S: 61    enivkyyGv..eveeeelkiflEyvegGslssllekygkleesvikkytkqileglayLH  118

         e+iv+yyG+  ++ e+ l+if+Ey++gGs++++l++yg+l+esv++kyt+qileg++yLH

Q: 422   ERIVQYYGClrDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLH  481

         *********76677889*******************************************

S: 119   ekkivHRDiKgaNilldssgvvklsDFGaskrlaelntkaaelesltGtlaymaPEvikk  178

         +++ivHRDiKgaNil ds+g+vkl+DFGaskrl++++++ +  +s+tGt+++m+PEvi++

Q: 482   SNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISG  541

         **********************************************************99

S: 179   kekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklseegkd  238

         +  gygrkaD+Wslgc+v+emlt+k+P+ae+ e++a++fk++++ t+P++p+++se+g+d

Q: 542   E--GYGRKADVWSLGCTVVEMLTEKPPWAEY-EAMAAIFKIATQPTNPQLPSHISEHGRD  598

         8..****************************.9***************************

S: 239   flekcleldpkkrpsaeeLLkhef  262

         fl++++ +++++rpsaeeLL+h+f

Q: 599   FLRRIF-VEARQRPSAEELLTHHF  621

         ******.8999***********99

Domain Sequence
(FASTA)

WRRGKLLGQG AFGRVYLCYD VDTGRELASK QVQFDPDSPE TSKEVSALEC EIQLLKNLQH 60

ERIVQYYGCL RDRAEKILTI FMEYMPGGSV KDQLKAYGAL TESVTRKYTR QILEGMSYLH 120

SNMIVHRDIK GANILRDSAG NVKLGDFGAS KRLQTICMSG TGIRSVTGTP YWMSPEVISG 180

EGYGRKADVW SLGCTVVEML TEKPPWAEYE AMAAIFKIAT QPTNPQLPSH ISEHGRDFLR 240

RIFVEARQRP SAEELLTHHF 260

Keyword

ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0046872	; F:metal ion binding
GO:0004672	; F:protein kinase activity
GO:0000186	; P:activation of MAPKK activity
GO:0000165	; P:MAPK cascade
GO:0046777	; P:protein autophosphorylation

KEGG

mmu:26406

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000270	; OPR_PB1.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.

Pfam

PF00564	; PB1; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00666	; PB1; 1.
SM00220	; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013