EPS-MUM-00018

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00018

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	293.0	6.0E-87	23	289	267

Status

Reviewed

Ensembl Protein

ENSMUSP00000021514

UniProt Accession

P49443;

Protein Name

Protein phosphatase 1A

Protein Synonyms/Alias

Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA;

Gene Name

Ppm1a

Gene Synonyms/Alias

Ppm1a; Pppm1a;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000021096	ENSMUSP00000021514	ENSMUST00000021514

Organism

Mus musculus

Functional Description

Enzyme with a broad specificity. Negatively regulatesTGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity).

Protein Length

382

Protein Sequence
(FASTA)

MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 60

GHAGSQVAKY CCEHLLDHIT NNQDFRGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 120

GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG 180

SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 240

IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPSAPKVSAE 300

AVKKEAELDK YLESRVEEII KKQVEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 360

VYNRLNPYKN DDTDSASTDD MW 382

Nucleotide Sequence
(FASTA)

ATGGGAGCAT TTTTAGACAA GCCAAAGATG GAGAAGCATA ATGCCCAGGG GCAGGGGAAT 60

GGGTTACGAT ACGGCCTAAG CAGCATGCAA GGTTGGCGAG TTGAAATGGA GGACGCACAC 120

ACGGCTGTGA TCGGTTTGCC AAGTGGACTT GAGACATGGT CATTCTTTGC TGTATATGAT 180

GGGCATGCTG GTTCTCAGGT TGCCAAATAC TGCTGTGAGC ACTTGTTAGA TCACATCACC 240

AATAACCAGG ATTTCAGAGG ATCTGCAGGA GCACCTTCTG TGGAGAACGT AAAGAATGGA 300

ATCAGAACAG GGTTTCTGGA GATTGATGAA CACATGAGAG TTATGTCAGA GAAGAAACAT 360

GGTGCAGATA GAAGCGGGTC AACAGCTGTG GGCGTCTTAA TCTCTCCCCA ACATACTTAT 420

TTCATTAACT GTGGAGACTC GAGAGGTTTA CTTTGTAGGA ATAGAAAAGT TCACTTCTTC 480

ACACAAGACC ATAAACCAAG TAACCCGCTG GAAAAAGAAC GAATTCAGAA TGCAGGGGGC 540

TCGGTGATGA TTCAGCGTGT CAATGGCTCT CTGGCTGTAT CGAGGGCCCT TGGGGATTTC 600

GATTACAAAT GTGTCCATGG AAAAGGTCCC ACAGAGCAGC TTGTCTCCCC AGAGCCCGAA 660

GTCCATGATA TTGAAAGGTC TGAAGAAGAT GACCAGTTCA TCATCCTTGC ATGCGATGGC 720

ATCTGGGACG TCATGGGGAA CGAAGAGCTC TGTGACTTTG TGAGATCCAG ACTTGAAGTC 780

ACTGATGACC TTGAGAAAGT TTGCAATGAA GTAGTCGACA CCTGCTTGTA TAAGGGAAGT 840

CGAGACAACA TGAGTGTGAT TTTGATCTGT TTTCCAAGTG CACCCAAAGT CTCGGCAGAG 900

GCGGTGAAGA AGGAGGCGGA GCTGGACAAG TACCTGGAGA GCAGAGTAGA AGAAATCATA 960

AAGAAGCAGG TGGAAGGCGT CCCTGACTTA GTCCACGTGA TGCGCACGTT AGCCAGTGAG 1020

AACATCCCCA GCCTCCCACC AGGGGGTGAA TTGGCAAGCA AGCGGAATGT AATTGAAGCC 1080

GTTTACAATA GACTGAACCC TTACAAAAAT GACGACACTG ATTCTGCGTC AACCGATGAT 1140

ATGTGGTAA 1149

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g++s++g+r emeda+t++ ++ +     + ++ff+vyDGh+gs+vak+c e+l +++

Q: 23    RYGLSSMQGWRVEMEDAHTAVIGLPSGL---ETWSFFAVYDGHAGSQVAKYCCEHLLDHI  79

         69****************9855554444...45***************************

S: 62    aeele....sseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdskl  117

         +++ +    +++++ ++vk+ ++++Fl++de+++v++ek++  d+sGstav +li++++

Q: 80    TNNQDfrgsAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHT  139

         ***999999999************************************************

S: 118   yvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLavsRa  177

         y++n+GdSr +Lcrn+k++  ++dHkps++ E+eRi++aggsv+ +   Rv+G+LavsRa

Q: 140   YFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQ---RVNGSLAVSRA  196

         **********************************************...***********

S: 178   lGdlylk.........pyvtpePevtshrltsede.fliLasDGlwdvvsneeavslvrd  227

         lGd+++k         ++v+pePev+ +++++ed+ f+iLa+DG+wdv+ nee++++vr+

Q: 197   LGDFDYKcvhgkgpteQLVSPEPEVHDIERSEEDDqFIILACDGIWDVMGNEELCDFVRS  256

         ******************************999999************************

S: 228   vlekkeaeqeaakkLvklalargskdnvsaivv  260

         +le +++++++++++v+++l +gs+dn+s+i++

Q: 257   RLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILI  289

         ****************************99976

Domain Sequence
(FASTA)

RYGLSSMQGW RVEMEDAHTA VIGLPSGLET WSFFAVYDGH AGSQVAKYCC EHLLDHITNN 60

QDFRGSAGAP SVENVKNGIR TGFLEIDEHM RVMSEKKHGA DRSGSTAVGV LISPQHTYFI 120

NCGDSRGLLC RNRKVHFFTQ DHKPSNPLEK ERIQNAGGSV MIQRVNGSLA VSRALGDFDY 180

KCVHGKGPTE QLVSPEPEVH DIERSEEDDQ FIILACDGIW DVMGNEELCD FVRSRLEVTD 240

DLEKVCNEVV DTCLYKGSRD NMSVILI 267

Keyword

Complete proteome; Hydrolase; Lipoprotein; Magnesium; Manganese; Metal-binding; Myristate; Nucleus; Phosphoprotein; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0043005	; C:neuron projection
GO:0005634	; C:nucleus
GO:0005891	; C:voltage-gated calcium channel complex
GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0000287	; F:magnesium ion binding
GO:0030145	; F:manganese ion binding
GO:0004721	; F:phosphoprotein phosphatase activity
GO:0070412	; F:R-SMAD binding
GO:0004871	; F:signal transducer activity
GO:0010991	; P:negative regulation of SMAD protein complex assembly
GO:0030512	; P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045893	; P:positive regulation of transcription, DNA-dependent
GO:0030177	; P:positive regulation of Wnt receptor signaling pathway
GO:0006470	; P:protein dephosphorylation
GO:0016055	; P:Wnt receptor signaling pathway

KEGG

mmu:19042

;

InterPros

IPR001932	; PP2C-like.
IPR012911	; PP2C_C.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481	; PP2C; 1.
PF07830	; PP2C_C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013