EKS-MUM-00448

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00448

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/DAPK	478.2	1.5E-143	14	275	262

Status

Reviewed

Ensembl Protein

ENSMUSP00000040825

UniProt Accession

Q80YE7; Q80YE6; Q8R341; Q8VDN6; Q9CSD4; Q9JJP7;

Protein Name

Death-associated protein kinase 1

Protein Synonyms/Alias

DAP kinase 1;

Gene Name

Dapk1

Gene Synonyms/Alias

Dapk1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000021559	ENSMUSP00000040825	ENSMUST00000044083
ENSMUSG00000021559	ENSMUSP00000076666	ENSMUST00000077453

Organism

Mus musculus

Functional Description

Calcium/calmodulin-dependent serine/threonine kinaseinvolved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3 (By similarity). Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death.

Protein Length

1430

Protein Sequence
(FASTA)

MTVFRQENVD DYYDTGEELG SGQFAVVKKC REKSTGLQYA AKFIKKRRTK SSRRGVSRED 60

IEREVSILKE IRHPNVITLH EVYENKTDVI LILELVAGGE LFDFLAEKES LTEEEATEFL 120

KQILSGVYYL HSLQIAHFDL KPENIMLLDR NVPKPRIKII DFGLAHKIDF GNEFKNIFGT 180

PEFVAPEIVN YEPLGLEADM WSIGVITYIL LSGASPFLGD TKQETLANVS AVNYDFEEEF 240

FRNTSTLAKD FIRRLLVKDP KKRMTIQDSL QHPWIKPKDT QQALSRKASA VNMEKFKKFA 300

ARKKWKQSVR LISLCQRLSR SFLSRSNMSV ARSDDTLDEE DSFVMKAIIH AINDDNVPGL 360

QHLLGSLSSY DVNQPNKHGT PPLLIAAGCG NIQMLQLLIK RGSRIDVQDK GGSNAIYWAS 420

RHGHVDTLKF LNENKCPLDV KDKSGETALH VAARYGHADV VQLLCSFGSN PDFQDKEEET 480

PLHCAAWHGY YSVAKALCEV GCNVNIKNRE GETPLLTASA RGYHDIVECL AEHGADLNAS 540

DKDGHIALHL AVRRCQMEVI KTLLGHGSFV DFQDRHGNTP LHVACKDGSA PIVVALCEAS 600

CNLDISNKYG RTPLHLAANN GILDVVRYLC LMGANVEALT SDGKTAEDLA KAEQHEHVAG 660

LLARLRKDTH RGLFIQQLRP TQNLQPRIKL KLFGHSGSGK STLVESLKCG LLRSFFRRRR 720

PRLSSTNSTR FPPSPLAAKP TVSVSINNLY PGCENVSVRS RSMMFEPGLT KGMLEVFVAP 780

SHHLHCSTDD QSTKAIDIQN AYLNGVGDFS VWEFSGNPVY FCCYDYFAAN DPTSIHIIVF 840

SLEEPYEIQL NQVIFWLSFL KSLVPVEEPI AFGGKLKNPL RVVLVATHAD IMNIPRPAGG 900

EFGYDKDTSL LKEIRNRFGN DLHVSNKLFV LDAGASGSKD IKVLRNHLQE IRSQIVSGCS 960

PMTHLCEKII STLPSWRKLN GPNQLMSLQQ FVYDVQDQLN PLASEDDLRR IAQQLHSTGE 1020

INIMQSETVQ DVLLLDPRWL CTNVLGKLLS VETPRALHHY RGRYTMEDIQ RLVPDSDVEE 1080

LLQILDAMDI CARDLSSGTM VDIPALIKTD SLQRSWADEE DEVMVYGGVR IVPVEHLTPF 1140

PCGIFHKVQV NLCRWIHQQS AEGDADIRLW VSGCRIANRG AELLVLLVNH GQGIEVQVRG 1200

LETEKIKCCL LLDSVCSTIE TVMATTLPGL LTVKHYLSPQ QLREHHEPVM VYQPRDFFRA 1260

QTLKESSLTN TMGGYKESFS SITCFGCHDV YSQASLGMDI HASDLSLLTR RKLSRLLDPP 1320

DPMGKDWCLL AMNLGLPDMV AKHNVNNRAS RDFLPSPVHA LLQEWTSYPE STVGILISKL 1380

RELGRRDAAD FLLKASSVFK INLDGNGQEA YASSCNSGTS YNSISSVVSR 1430

Nucleotide Sequence
(FASTA)

ATGACTGTGT TCAGGCAGGA AAACGTGGAC GACTACTACG ACACCGGCGA GGAACTGGGC 60

AGTGGACAGT TCGCAGTTGT GAAGAAATGT CGTGAGAAAA GTACCGGTCT TCAGTATGCG 120

GCCAAGTTCA TCAAGAAAAG GAGGACCAAG TCCAGCCGGC GGGGCGTGAG CCGGGAGGAC 180

ATCGAGCGGG AGGTCAGCAT CCTGAAGGAG ATCCGGCACC CAAATGTCAT CACCCTGCAT 240

GAGGTCTATG AGAACAAGAC AGATGTCATT CTGATCCTGG AGCTTGTTGC AGGAGGTGAG 300

CTGTTTGACT TCCTGGCTGA GAAGGAATCT CTGACTGAAG AGGAGGCAAC GGAATTCCTT 360

AAGCAGATTC TCAGCGGCGT TTACTACCTG CACTCACTGC AGATCGCTCA CTTTGACCTG 420

AAGCCGGAAA ACATAATGCT TCTGGATAGA AATGTGCCCA AACCTCGGAT CAAGATCATA 480

GACTTTGGCT TGGCCCATAA AATTGACTTT GGAAATGAAT TCAAAAACAT ATTTGGGACA 540

CCAGAGTTTG TGGCTCCGGA GATAGTCAAC TATGAGCCCC TGGGTCTTGA GGCAGATATG 600

TGGAGCATCG GGGTAATAAC CTATATCCTC CTAAGTGGGG CCTCCCCTTT TCTTGGAGAC 660

ACCAAGCAAG AAACATTAGC GAATGTGTCC GCTGTCAACT ACGACTTTGA GGAGGAATTC 720

TTCCGGAACA CCAGTACCCT TGCCAAAGAT TTCATCAGAA GACTGCTGGT CAAGGATCCA 780

AAGAAGAGGA TGACAATCCA GGACAGTTTG CAGCACCCCT GGATCAAGCC TAAAGACACC 840

CAACAAGCAC TTAGTCGAAA AGCCTCAGCA GTAAACATGG AGAAATTCAA GAAGTTTGCA 900

GCTCGGAAAA AATGGAAACA ATCTGTTCGC TTGATATCAC TGTGCCAAAG ATTATCCAGG 960

TCATTTTTGT CCAGAAGTAA CATGAGTGTC GCCAGGAGTG ATGATACTCT GGATGAGGAA 1020

GACTCCTTTG TGATGAAAGC CATCATCCAT GCCATCAATG ATGACAACGT ACCCGGCCTG 1080

CAGCATCTCC TGGGCTCCTT GTCCAGCTAT GACGTCAACC AGCCCAACAA GCATGGGACA 1140

CCTCCATTAC TGATTGCCGC AGGCTGTGGC AACATCCAGA TGTTACAGTT ACTCATAAAA 1200

CGAGGCTCAA GGATTGACGT CCAGGATAAG GGAGGATCCA ATGCCATCTA CTGGGCCTCT 1260

CGGCATGGCC ATGTGGATAC TTTGAAATTT CTCAATGAGA ACAAATGCCC TTTGGATGTT 1320

AAAGACAAGT CTGGAGAGAC AGCTCTTCAC GTGGCAGCCC GCTATGGCCA TGCAGATGTG 1380

GTTCAACTAC TGTGCAGTTT TGGCTCTAAT CCTGATTTCC AGGACAAGGA AGAGGAAACC 1440

CCCCTGCACT GTGCTGCCTG GCATGGCTAT TACTCCGTGG CTAAAGCTCT TTGTGAAGTT 1500

GGCTGCAACG TGAATATCAA GAATCGGGAG GGAGAGACCC CATTGCTGAC GGCGTCTGCC 1560

AGGGGCTATC ATGACATTGT GGAGTGTCTG GCTGAACATG GAGCTGACTT GAATGCTTCT 1620

GACAAGGATG GACACATCGC TCTTCATCTT GCTGTGAGGC GTTGTCAGAT GGAAGTCATC 1680

AAGACCCTCC TTGGCCATGG GTCCTTTGTG GATTTCCAGG ACAGGCATGG CAACACACCC 1740

CTGCACGTGG CCTGCAAAGA TGGAAGCGCA CCTATCGTGG TGGCCCTCTG TGAAGCCAGC 1800

TGCAATCTGG ACATCTCAAA CAAGTATGGT CGGACTCCTC TCCACCTTGC AGCCAACAAC 1860

GGGATCCTAG ATGTGGTCCG CTACCTCTGT TTGATGGGCG CCAATGTGGA GGCTCTAACC 1920

TCGGATGGAA AGACGGCCGA GGACCTCGCC AAGGCAGAAC AGCACGAGCA TGTGGCAGGG 1980

CTCCTGGCAA GACTGCGGAA GGACACACAC CGAGGACTCT TCATCCAGCA ACTCCGACCC 2040

ACCCAGAATC TCCAGCCCAG AATCAAGCTC AAACTGTTTG GCCATTCGGG ATCAGGGAAA 2100

TCCACCCTGG TGGAATCTCT CAAGTGTGGG CTGTTAAGGA GTTTCTTCAG AAGGCGCCGG 2160

CCCAGACTAT CCTCTACCAA CTCCACCCGC TTCCCACCGT CACCCCTGGC TGCTAAGCCA 2220

ACAGTCTCAG TGAGCATTAA CAACCTGTAC CCCGGCTGTG AGAACGTGAG CGTAAGGAGC 2280

CGCAGCATGA TGTTCGAGCC GGGCCTCACC AAAGGGATGC TGGAAGTGTT CGTGGCTCCG 2340

TCTCACCACC TCCACTGCTC GACTGATGAC CAGTCCACCA AAGCCATCGA CATCCAGAAT 2400

GCTTATTTGA ACGGAGTTGG TGATTTCAGT GTGTGGGAGT TCTCTGGAAA CCCTGTGTAC 2460

TTCTGTTGCT ATGACTACTT TGCTGCCAAC GACCCCACGT CCATCCACAT CATCGTTTTC 2520

AGTCTCGAAG AACCCTATGA GATCCAGCTG AACCAAGTGA TTTTCTGGCT CAGTTTCCTG 2580

AAGTCTCTGG TCCCAGTTGA AGAACCCATA GCATTTGGAG GCAAGCTGAA GAACCCTCTC 2640

CGAGTTGTCC TGGTGGCAAC ACATGCTGAC ATCATGAACA TCCCTCGGCC TGCTGGAGGC 2700

GAGTTTGGAT ATGACAAGGA TACATCCTTG CTGAAAGAGA TCAGAAACAG GTTCGGGAAT 2760

GACCTTCATG TCTCAAACAA GCTGTTTGTC CTGGATGCAG GAGCGTCTGG GTCTAAGGAC 2820

ATTAAGGTTC TTCGGAATCA CCTGCAAGAA ATACGGAGCC AGATTGTCTC GGGGTGTTCG 2880

CCCATGACTC ACCTGTGCGA GAAGATCATC TCCACGCTGC CCTCCTGGCG GAAGCTCAAC 2940

GGGCCAAACC AGCTGATGTC GCTGCAGCAG TTTGTGTATG ACGTGCAGGA CCAGCTGAAC 3000

CCCCTGGCCT CTGAGGATGA CCTCAGGCGC ATTGCACAAC AGCTACACAG CACGGGCGAG 3060

ATAAACATCA TGCAGAGTGA GACAGTGCAG GACGTGTTAC TGCTGGATCC TCGATGGCTC 3120

TGCACCAACG TCCTGGGGAA GCTGCTGTCT GTGGAGACGC CCCGAGCCCT GCACCATTAC 3180

CGGGGCCGCT ACACCATGGA AGACATCCAG CGTCTGGTCC CAGACAGTGA CGTGGAGGAG 3240

CTGCTGCAGA TACTGGATGC CATGGACATC TGCGCCCGAG ACCTGAGTAG TGGGACTATG 3300

GTGGATATCC CTGCTCTGAT CAAAACAGAC AGCCTGCAGC GCTCCTGGGC GGATGAGGAG 3360

GATGAGGTGA TGGTGTACGG TGGGGTGCGC ATTGTCCCTG TAGAGCACCT CACCCCCTTC 3420

CCCTGTGGCA TCTTTCACAA AGTTCAGGTC AACCTGTGCC GATGGATCCA CCAGCAAAGC 3480

GCGGAGGGTG ATGCGGACAT CCGTCTGTGG GTGAGCGGCT GCAGGATCGC CAACCGCGGG 3540

GCTGAGTTGC TGGTGCTGCT GGTCAATCAC GGTCAGGGCA TCGAGGTACA GGTGCGTGGC 3600

CTGGAGACGG AGAAGATTAA GTGCTGCCTG CTGCTGGACT CGGTGTGCAG CACCATCGAG 3660

ACCGTCATGG CCACCACCTT GCCAGGGTTG TTGACGGTGA AGCACTACCT GAGCCCCCAG 3720

CAGCTAAGGG AGCACCACGA ACCGGTCATG GTCTATCAGC CCCGGGACTT CTTCCGTGCG 3780

CAGACCCTGA AAGAGAGTTC CCTTACCAAC ACCATGGGTG GGTACAAGGA GAGCTTCAGC 3840

AGCATCACGT GCTTTGGGTG TCATGACGTC TACTCACAGG CCAGTCTTGG CATGGATATC 3900

CATGCGTCAG ACCTGAGTCT CCTGACCCGG AGGAAACTGA GTCGCCTGCT TGACCCACCC 3960

GACCCCATGG GGAAGGACTG GTGCCTTCTG GCCATGAACT TGGGCCTCCC AGACATGGTG 4020

GCCAAACACA ACGTCAACAA CAGGGCTTCT AGGGATTTCC TCCCTAGCCC AGTGCATGCC 4080

TTGCTACAGG AATGGACCTC CTACCCTGAG AGCACGGTGG GCATCCTTAT ATCCAAACTT 4140

CGGGAGCTGG GGCGCCGGGA TGCTGCGGAC TTTTTACTGA AGGCCTCCTC TGTGTTCAAG 4200

ATCAACCTTG ATGGCAATGG CCAGGAGGCC TATGCCTCAA GCTGTAACAG TGGCACGTCC 4260

TACAATTCCA TTAGCTCAGT GGTGTCCCGG TGA 4293

Domain Profile

S: 2     etgkelgsGkfavvrkcrekstGkeyaakflkkrrlkssrrgvsreeierevavlkeirs  61

         +tg+elgsG+favv+kcrekstG +yaakf+kkrr+kssrrgvsre+ierev++lkeir

Q: 14    DTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIR-  72

         789********************************************************.

S: 62    epnvinlhevyenksdvililelvaGGelfdllaekeslseeeatellkqilegvkylhs  121

         +pnvi+lhevyenk+dvililelvaGGelfd+laekesl+eeeate+lkqil+gv+ylhs

Q: 73    HPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHS  132

         ************************************************************

S: 122   kkivhldlkPenillldkkvpkgdiklidfGlsrkieegaelkeilGtPefvaPeivnye  181

          +i+h+dlkPeni+lld++vpk++ik+idfGl++ki+ g+e+k+i+GtPefvaPeivnye

Q: 133   LQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYE  192

         ************************************************************

S: 182   plsletdmwsiGvityillsgasPflGdtkqetllnisavnldfseelfssvselakdfi  241

         pl+le+dmwsiGvityillsgasPflGdtkqetl+n+savn+df+ee+f+++s+lakdfi

Q: 193   PLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFI  252

         ************************************************************

S: 242   rrllvkdpekrltieeclehswl  264

         rrllvkdp+kr+ti+++l+h+w+

Q: 253   RRLLVKDPKKRMTIQDSLQHPWI  275

         **********************8

Domain Sequence
(FASTA)

DTGEELGSGQ FAVVKKCREK STGLQYAAKF IKKRRTKSSR RGVSREDIER EVSILKEIRH 60

PNVITLHEVY ENKTDVILIL ELVAGGELFD FLAEKESLTE EEATEFLKQI LSGVYYLHSL 120

QIAHFDLKPE NIMLLDRNVP KPRIKIIDFG LAHKIDFGNE FKNIFGTPEF VAPEIVNYEP 180

LGLEADMWSI GVITYILLSG ASPFLGDTKQ ETLANVSAVN YDFEEEFFRN TSTLAKDFIR 240

RLLVKDPKKR MTIQDSLQHP WI 262

Keyword

Alternative splicing; ANK repeat; Apoptosis; ATP-binding; Calmodulin-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0015629	; C:actin cytoskeleton
GO:0005829	; C:cytosol
GO:0005524	; F:ATP binding
GO:0005516	; F:calmodulin binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0097190	; P:apoptotic signaling pathway
GO:0006917	; P:induction of apoptosis
GO:0007243	; P:intracellular protein kinase cascade
GO:0043066	; P:negative regulation of apoptotic process
GO:0043065	; P:positive regulation of apoptotic process
GO:0006468	; P:protein phosphorylation
GO:2000310	; P:regulation of N-methyl-D-aspartate selective glutamate receptor activity

KEGG

mmu:69635

;

InterPros

IPR002110	; Ankyrin_rpt.
IPR020683	; Ankyrin_rpt-contain_dom.
IPR000488	; Death.
IPR020676	; Death-assoc_prot_kinase.
IPR011029	; DEATH-like.
IPR011009	; Kinase-like_dom.
IPR020675	; Myosin_light_ch_kinase-rel.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00023	; Ank; 1.
PF12796	; Ank_2; 2.
PF00531	; Death; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00248	; ANK; 9.
SM00005	; DEATH; 1.
SM00220	; S_TKc; 1.

Prosites

PS50297	; ANK_REP_REGION; 1.
PS50088	; ANK_REPEAT; 7.
PS50017	; DEATH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR01415

; ANKYRIN.

Created Date

20-Feb-2013