EKS-MUM-00292

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00292

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	428.1	3.0E-128	79	331	253

Status

Reviewed

Ensembl Protein

ENSMUSP00000022212

UniProt Accession

P53351;

Protein Name

Serine/threonine-protein kinase PLK2

Protein Synonyms/Alias

Polo-like kinase 2; PLK-2; Serine/threonine-protein kinase SNK; Serum-inducible kinase;

Gene Name

Plk2

Gene Synonyms/Alias

Plk2; Snk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000021701	ENSMUSP00000022212	ENSMUST00000022212

Organism

Mus musculus

Functional Description

Tumor suppressor serine/threonine-protein kinaseinvolved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.

Protein Length

682

Protein Sequence
(FASTA)

MELLRTITYQ PAAGTKMCEQ ALGKACGGDS KKKRPQQPSE DGQPQAQVTP AAPHHHHHHS 60

HSGPEISRII VDPTTGKRYC RGKVLGKGGF AKCYEMTDLT NNKVYAAKII PHSRVAKPHQ 120

REKIDKEIEL HRLLHHKHVV QFYHYFEDKE NIYILLEYCS RRSMAHILKA RKVLTEPEVR 180

YYLRQIVSGL KYLHEQEILH RDLKLGNFFI NEAMELKVGD FGLAARLEPL EHRRRTICGT 240

PNYLSPEVLN KQGHGCESDI WALGCVMYTM LLGRPPFETT NLKETYRCIR EARYTMPSSL 300

LAPAKHLIAS MLSKNPEDRP SLDDIIRHDF FLQGFTPDRL SSSCCHTVPD FHLSSPAKNF 360

FKKAAAALFG GKKDKARYND THNKVSKEDE DIYKLRHDLK KVSITQQPSK HRADEEPQPP 420

PTTVARSGTS AVENKQQIGD AIRMIVRGTL GSCSSSSECL EDSTMGSVAD TVARVLRGCL 480

ENMPEADCIP KEQLSTSFQW VTKWVDYSNK YGFGYQLSDH TVGVLFNNGA HMSLLPDKKT 540

VHYYAELGQC SVFPATDAPE QFISQVTVLK YFSHYMEENL MDGGDLPSVT DIRRPRLYLL 600

QWLKSDKALM MLFNDGTFQV NFYHDHTKII ICNQSEEYLL TYINEDRIST TFRLTTLLMS 660

GCSLELKNRM EYALNMLLQR CN 682

Nucleotide Sequence
(FASTA)

ATGGAGCTCC TGCGGACTAT CACCTACCAG CCGGCCGCCG GCACCAAGAT GTGCGAGCAG 60

GCTCTGGGCA AAGCTTGCGG CGGGGACTCA AAGAAGAAGC GACCGCAGCA GCCTTCTGAA 120

GATGGGCAGC CCCAAGCCCA GGTGACCCCG GCGGCCCCGC ACCACCATCA CCACCATTCC 180

CACTCGGGAC CCGAGATCTC GCGGATTATA GTCGACCCCA CGACGGGGAA GCGCTACTGC 240

CGGGGCAAAG TGCTGGGCAA GGGTGGATTT GCAAAGTGTT ACGAAATGAC AGATCTGACA 300

AACAACAAAG TCTACGCTGC AAAAATTATT CCTCACAGCA GAGTAGCTAA ACCTCATCAG 360

AGGGAAAAGA TCGACAAAGA AATCGAGCTT CACAGACTAC TGCACCATAA GCATGTCGTG 420

CAGTTTTACC ACTACTTTGA AGACAAAGAA AACATTTACA TTCTCTTGGA ATACTGCAGT 480

AGAAGGTCCA TGGCTCACAT CTTGAAAGCA AGAAAGGTGT TGACAGAGCC AGAAGTCCGA 540

TACTACCTCA GGCAGATTGT GTCAGGACTC AAGTATCTTC ACGAACAAGA AATCTTGCAC 600

AGGGATCTCA AGCTAGGGAA CTTTTTTATT AATGAAGCCA TGGAGCTGAA GGTGGGAGAC 660

TTTGGTTTGG CAGCCAGACT GGAACCACTG GAACACAGAA GGAGAACAAT ATGTGGAACC 720

CCAAATTATC TCTCCCCCGA AGTCCTCAAC AAACAAGGAC ACGGCTGTGA ATCAGACATC 780

TGGGCCTTAG GCTGTGTAAT GTATACGATG CTGCTAGGAA GACCTCCATT CGAAACCACA 840

AATCTGAAAG AAACGTACAG GTGCATAAGG GAAGCAAGGT ATACCATGCC ATCCTCATTG 900

CTGGCCCCTG CTAAGCACTT GATAGCTAGC ATGCTGTCCA AAAACCCAGA GGACCGCCCC 960

AGTTTGGATG ACATCATTCG GCATGACTTC TTCCTGCAGG GTTTCACTCC GGACAGACTC 1020

TCTTCCAGCT GTTGCCACAC AGTTCCAGAT TTCCACTTGT CAAGCCCAGC CAAGAATTTC 1080

TTTAAGAAAG CCGCAGCCGC TCTTTTTGGT GGCAAGAAGG ACAAAGCAAG ATATAACGAC 1140

ACACACAATA AGGTGTCTAA GGAAGATGAA GACATTTACA AGCTTCGGCA TGATTTGAAG 1200

AAAGTGTCGA TAACCCAGCA GCCTAGCAAA CACAGAGCAG ATGAGGAGCC CCAGCCGCCT 1260

CCCACTACTG TTGCCAGATC TGGAACGTCC GCAGTGGAAA ACAAACAGCA GATTGGGGAT 1320

GCAATCCGGA TGATAGTCAG GGGGACTCTC GGCAGCTGCA GCAGCAGCAG CGAATGCCTT 1380

GAAGACAGCA CCATGGGAAG TGTGGCAGAC ACAGTGGCAA GAGTCCTTCG AGGATGTCTA 1440

GAAAACATGC CGGAAGCTGA CTGTATCCCC AAAGAGCAGC TGAGCACGTC CTTTCAGTGG 1500

GTCACCAAGT GGGTCGACTA CTCCAACAAA TACGGCTTTG GGTACCAGCT CTCGGACCAC 1560

ACTGTTGGCG TCCTTTTCAA CAACGGGGCT CACATGAGCC TCCTTCCGGA CAAAAAGACA 1620

GTTCACTATT ATGCGGAACT TGGCCAATGC TCTGTTTTCC CAGCAACAGA TGCCCCTGAA 1680

CAATTTATTA GTCAAGTGAC GGTGCTGAAA TACTTTTCTC ATTACATGGA GGAGAACCTC 1740

ATGGATGGTG GTGATCTCCC GAGTGTTACT GACATTCGAA GACCTCGGCT CTACCTCCTG 1800

CAGTGGTTAA AGTCTGATAA AGCCTTAATG ATGCTCTTCA ATGACGGCAC ATTTCAGGTG 1860

AATTTCTACC ACGATCATAC AAAAATCATC ATCTGTAACC AGAGTGAAGA ATACCTTCTC 1920

ACCTACATCA ATGAGGACAG GATCTCTACA ACTTTCAGAC TGACGACTCT GCTGATGTCT 1980

GGCTGTTCGT TAGAATTGAA AAATCGAATG GAATATGCCC TGAACATGCT CTTACAGAGA 2040

TGTAACTGA 2049

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y+rgk+lGkGgfakcye++dl++++v+a+k++p+s++ak++qrek++keie+hr l+hk+

Q: 79    YCRGKVLGKGGFAKCYEMTDLTNNKVYAAKIIPHSRVAKPHQREKIDKEIELHRLLHHKH  138

         99**********************************************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         +v++y++fedken+y++le+csrrs+a++lk+rk+ltepevryylrqivsglkylhe++i

Q: 139   VVQFYHYFEDKENIYILLEYCSRRSMAHILKARKVLTEPEVRYYLRQIVSGLKYLHEQEI  198

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghslev  180

         lhrdlklgn+f+ne++e+k+gdfGla+rle+ e+r++t+cGtPny++Pevl+k+gh++e+

Q: 199   LHRDLKLGNFFINEAMELKVGDFGLAARLEPLEHRRRTICGTPNYLSPEVLNKQGHGCES  258

         ************************************************************

S: 181   diWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePee  240

         diW+lGc++yt+l+G+PPfet++lkety++i++a+y++Pssl ++ak+lia++l+k+Pe+

Q: 259   DIWALGCVMYTMLLGRPPFETTNLKETYRCIREARYTMPSSLLAPAKHLIASMLSKNPED  318

         ************************************************************

S: 241   rpsleevlkdefl  253

         rpsl+++++++f+

Q: 319   RPSLDDIIRHDFF  331

         ************8

Domain Sequence
(FASTA)

YCRGKVLGKG GFAKCYEMTD LTNNKVYAAK IIPHSRVAKP HQREKIDKEI ELHRLLHHKH 60

VVQFYHYFED KENIYILLEY CSRRSMAHIL KARKVLTEPE VRYYLRQIVS GLKYLHEQEI 120

LHRDLKLGNF FINEAMELKV GDFGLAARLE PLEHRRRTIC GTPNYLSPEV LNKQGHGCES 180

DIWALGCVMY TMLLGRPPFE TTNLKETYRC IREARYTMPS SLLAPAKHLI ASMLSKNPED 240

RPSLDDIIRH DFF 253

Keyword

ATP-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005814	; C:centriole
GO:0030425	; C:dendrite
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004871	; F:signal transducer activity
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0060292	; P:long term synaptic depression
GO:0060291	; P:long-term synaptic potentiation
GO:0007613	; P:memory
GO:0007093	; P:mitotic cell cycle checkpoint
GO:0007052	; P:mitotic spindle organization
GO:0043066	; P:negative regulation of apoptotic process
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0032486	; P:Rap protein signal transduction
GO:0046599	; P:regulation of centriole replication

KEGG

mmu:20620

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013