| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00180 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| AGC/PKC | 439.4 | 1.2E-131 | 232 | 486 | 255 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000107822 |
| UniProt Accession | P28867; Q91V85; Q9Z333; |
| Protein Name | Protein kinase C delta type |
| Protein Synonyms/Alias | Tyrosine-protein kinase PRKCD; nPKC-delta; |
| Gene Name | Prkcd |
| Gene Synonyms/Alias | Prkcd; Pkcd; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Calcium-independent, phospholipid- and diacylglycerol(DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor- initiated cell death, is involved in tumor suppression, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Upon DNA damage, activates the promoter of the death- promoting transcription factor BCLAF1/Btf to trigger BCLAF1- mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl- leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C- terminal and regulates the interaction between MUC1 and beta- catenin. |
| Protein Length | 545 |
Protein Sequence
(FASTA) | | MCVQYFLEDG DCKQSMRSEE EAKFPTMNRR GAIKQAKIHY IKNHEFIATF FGQPTFCSVC 60 | | KEFVWGLNKQ GYKCRQCNAA IHKKCIDKII GRCTGTATNS RDTIFQKERF NIDMPHRFKV 120 | | YNYMSPTFCD HCGSLLWGLV KQGLKCEDCG MNVHHKCREK VANLCGINQK LLAEALNQVT 180 | | QRSSRKLDTT ESVGIYQGFE KKPEVSGSDI LDNNGTYGKI WEGSTRCTLE NFTFQKVLGK 240 | | GSFGKVLLAE LKGKDKYFAI KCLKKDVVLI DDDVECTMVE KRVLALAWES PFLTHLICTF 300 | | QTKDHLFFVM EFLNGGDLMF HIQDKGRFEL YRATFYAAEI ICGLQFLHSK GIIYRDLKLD 360 | | NVMLDRDGHI KIADFGMCKE NIFGEGRAST FCGTPDYIAP EILQGLKYSF SVDWWSFGVL 420 | | LYEMLIGQSP FHGDDEDELF ESIRVDTPHY PRWITKESKD IMEKLFERDP DKRLGVTGNI 480 | | RIHPFFKTIN WSLLEKRKVE PPFKPKVQAC FLTPPGMLCM YHSLYSCILA ISALHSFRRQ 540 | | LELQI 545 |
|
Nucleotide Sequence
(FASTA) | | ATGTGTGTGC AGTATTTCCT GGAGGATGGG GATTGCAAAC AGTCTATGCG TAGTGAGGAG 60 | | GAGGCAAAGT TTCCAACCAT GAACCGTCGT GGAGCCATTA AACAGGCCAA GATCCACTAC 120 | | ATCAAGAACC ACGAGTTTAT CGCCACCTTC TTCGGGCAGC CCACCTTCTG TTCTGTGTGC 180 | | AAAGAGTTTG TCTGGGGCCT CAACAAGCAA GGCTACAAAT GCAGGCAATG CAACGCTGCC 240 | | ATCCACAAGA AATGCATTGA CAAGATTATC GGCCGCTGCA CTGGCACTGC CACCAATAGC 300 | | CGGGACACCA TCTTCCAGAA AGAACGCTTC AACATCGACA TGCCTCACCG ATTCAAGGTT 360 | | TATAACTACA TGAGCCCCAC CTTCTGTGAC CACTGTGGCA GTTTGCTCTG GGGACTGGTG 420 | | AAGCAGGGAT TAAAGTGTGA AGATTGTGGC ATGAATGTGC ACCACAAATG CCGGGAGAAG 480 | | GTGGCCAACC TGTGTGGTAT CAACCAAAAG CTCTTGGCTG AGGCCTTGAA CCAAGTGACC 540 | | CAGAGATCTT CCCGGAAGCT GGACACAACA GAGTCTGTCG GAATATACCA GGGATTTGAG 600 | | AAGAAGCCAG AAGTCTCTGG GAGTGACATC CTAGACAACA ACGGGACCTA TGGCAAGATC 660 | | TGGGAGGGGA GCACCCGGTG CACCCTTGAG AACTTCACCT TCCAAAAAGT ACTTGGCAAA 720 | | GGCAGCTTTG GCAAGGTGCT GCTGGCAGAG CTGAAGGGCA AAGACAAGTA CTTTGCAATC 780 | | AAGTGTCTGA AGAAGGACGT GGTGTTGATT GACGATGATG TAGAGTGTAC CATGGTGGAG 840 | | AAGCGGGTGC TGGCGCTCGC CTGGGAGAGT CCCTTCCTCA CCCACCTCAT CTGTACCTTC 900 | | CAGACCAAGG ACCACCTGTT CTTCGTGATG GAGTTTCTCA ATGGGGGTGA CCTGATGTTC 960 | | CACATTCAGG ACAAAGGCCG CTTCGAACTC TACCGGGCTA CGTTTTATGC AGCTGAGATC 1020 | | ATCTGCGGAC TGCAGTTTCT ACACAGCAAA GGCATTATTT ACAGGGACCT CAAGCTGGAC 1080 | | AATGTGATGC TAGACAGGGA CGGCCACATC AAGATCGCTG ACTTTGGGAT GTGCAAAGAG 1140 | | AATATATTTG GGGAGGGCCG GGCCAGCACA TTCTGCGGCA CTCCTGACTA CATCGCCCCT 1200 | | GAGATCCTGC AGGGCCTGAA GTACTCCTTC TCGGTGGACT GGTGGTCTTT CGGGGTCCTC 1260 | | CTGTACGAAA TGCTCATCGG CCAGTCCCCC TTCCACGGCG ACGATGAGGA CGAGCTCTTC 1320 | | GAGTCCATCC GGGTGGACAC ACCACACTAT CCCCGTTGGA TCACCAAGGA ATCCAAGGAC 1380 | | ATCATGGAGA AGCTATTCGA GAGGGACCCT GACAAGAGGC TGGGAGTAAC AGGAAACATC 1440 | | AGGATTCACC CCTTTTTCAA GACTATCAAC TGGTCCCTCC TGGAGAAGCG GAAGGTGGAG 1500 | | CCGCCCTTTA AGCCCAAAGT GCAAGCCTGC TTCCTGACCC CTCCTGGCAT GCTGTGTATG 1560 | | TACCACAGCC TCTACTCTTG CATCCTCGCA ATCTCAGCCC TGCACTCATT CAGGAGACAG 1620 | | CTGGAACTGC AGATTTAG 1638 |
|
| Domain Profile | S: 1 fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp 60 | f++ kvlGkGsfgkvllaelk++d+++aik+lkkdvvl dddve+t+vekrvlala+++p | Q: 232 FTFQKVLGKGSFGKVLLAELKGKDKYFAIKCLKKDVVLIDDDVECTMVEKRVLALAWESP 291 | 89********************************************************** |
| S: 61 flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg 120 | fl++l+++fqt+d+lffv+e++nGGdlmfhiq++++++ ra+fyaaeii++l+flh+kg | Q: 292 FLTHLICTFQTKDHLFFVMEFLNGGDLMFHIQDKGRFELYRATFYAAEIICGLQFLHSKG 351 | ************************************************************ |
| S: 121 iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks 180 | iiyrdlkldnv+ld++Ghik+adfG+cke+i+ + ++stfcGtPdyiaPeil++ +y++s | Q: 352 IIYRDLKLDNVMLDRDGHIKIADFGMCKENIFGEGRASTFCGTPDYIAPEILQGLKYSFS 411 | ************************************************************ |
| S: 181 vdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkglltkdpe 240 | vdww++Gvllyeml+gqsPf+g+dedelfesi+ ++++yP++++ke+++i+++l+++dp+ | Q: 412 VDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDIMEKLFERDPD 471 | ************************************************************ |
| S: 241 krlGvkeegeedikehaff 259 | krlGv +g +i+ h+ff | Q: 472 KRLGV--TG--NIRIHPFF 486 | *****..56..79999998 |
|
Domain Sequence
(FASTA) | | FTFQKVLGKG SFGKVLLAEL KGKDKYFAIK CLKKDVVLID DDVECTMVEK RVLALAWESP 60 | | FLTHLICTFQ TKDHLFFVME FLNGGDLMFH IQDKGRFELY RATFYAAEII CGLQFLHSKG 120 | | IIYRDLKLDN VMLDRDGHIK IADFGMCKEN IFGEGRASTF CGTPDYIAPE ILQGLKYSFS 180 | | VDWWSFGVLL YEMLIGQSPF HGDDEDELFE SIRVDTPHYP RWITKESKDI MEKLFERDPD 240 | | KRLGVTGNIR IHPFF 255 |
|
| Keyword | 3D-structure; Alternative splicing; Apoptosis; ATP-binding; Cell cycle; Complete proteome; Cytoplasm; Endoplasmic reticulum; Kinase; Membrane; Metal-binding; Mitochondrion; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Tumor suppressor; Zinc; Zinc-finger. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |