EKS-MUM-00157

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00157

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/NDR	508.2	1.6E-152	626	931	306

Status

Reviewed

Ensembl Protein

ENSMUSP00000022531

UniProt Accession

Q7TSJ6; Q8CDJ4; Q9JMI3;

Protein Name

Serine/threonine-protein kinase LATS2

Protein Synonyms/Alias

Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; Serine/threonine-protein kinase kpm;

Gene Name

Lats2

Gene Synonyms/Alias

Lats2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000021959	ENSMUSP00000038982	ENSMUST00000038381
ENSMUSG00000021959	ENSMUSP00000133680	ENSMUST00000174694
ENSMUSG00000021959	ENSMUSP00000133976	ENSMUST00000173990
ENSMUSG00000021959	ENSMUSP00000022531	ENSMUST00000022531
ENSMUSG00000021959	ENSMUSP00000077130	ENSMUST00000077981
ENSMUSG00000021959	ENSMUSP00000133379	ENSMUST00000174166
ENSMUSG00000021959	ENSMUSP00000133857	ENSMUST00000173732
ENSMUSG00000021959	ENSMUSP00000134142	ENSMUST00000173964
ENSMUSG00000021959	ENSMUSP00000134321	ENSMUST00000174213

Organism

Mus musculus

Functional Description

Negative regulator of YAP1 in the Hippo signalingpathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor (By similarity).

Protein Length

1042

Protein Sequence
(FASTA)

MRPKTFPATT YSGNSRQRLQ EIREGLKQPS KASTQGLLVG PNSDTSLDAK VLGSKDASRQ 60

QQMRATPKFG PYQKALREIR YSLLPFANES GTSAAAEVNR QMLQELVNAG CDQEMAGRAL 120

KQTGSRSIEA ALEYISKMGY LDPRNEQIVR VIKQTSPGKG LAPTPVTRRP SFEGTGEALP 180

SYHQLGGANY EGPAALEEMP RQYLDFLFPG AGAGTHGAQA HQHPPKGYST AVEPSAHFPG 240

THYGRGHLLS EQPGYGVQRS SSFQNKTPPD AYSSMAKAQG GPPASLTFPA HAGLYTASHH 300

KPAATPPGAH PLHVLGTRGP TFTGESSAQA VLAPSRNSLN ADLYELGSTV PWSAAPLARR 360

DSLQKQGLEA SRPHVAFRAG PSRTNSFNNP QPEPSLPAPN TVTAVTAAHI LHPVKSVRVL 420

RPEPQTAVGP SHPAWVAAPT APATESLETK EGSAGPHPLD VDYGGSERRC PPPPYPKHLL 480

LPSKSEQYSV DLDSLCTSVQ QSLRGGTEQD RSDKSHKGAK GDKAGRDKKQ IQTSPVPVRK 540

NSRDEEKRES RIKSYSPYAF KFFMEQHVEN VIKTYQQKVS RRLQLEQEMA KAGLCEAEQE 600

QMRKILYQKE SNYNRLKRAK MDKSMFVKIK TLGIGAFGEV CLACKLDTHA LYAMKTLRKK 660

DVLNRNQVAH VKAERDILAE ADNEWVVKLY YSFQDKDSLY FVMDYIPGGD MMSLLIRMEV 720

FPEHLARFYI AELTLAIESV HKMGFIHRDI KPDNILIDLD GHIKLTDFGL CTGFRWTHNS 780

KYYQKGNHMR QDSMEPGDLW DDVSNCRCGD RLKTLEQRAQ KQHQRCLAHS LVGTPNYIAP 840

EVLLRKGYTQ LCDWWSVGVI LFEMLVGQPP FLAPTPTETQ LKVINWESTL HIPTQVRLSA 900

EARDLITKLC CAADCRLGRD GADDLKAHPF FNTIDFSRDI RKQPAPYVPT ISHPMDTSNF 960

DPVDEESPWH EASGESAKAW DTLASPSSKH PEHAFYEFTF RRFFDDNGYP FRCPKPSEPA 1020

ESADPGDADL EGAAEGCQPV YV 1042

Nucleotide Sequence
(FASTA)

ATGAGGCCAA AGACTTTTCC TGCCACAACT TACTCTGGAA ATAGCCGGCA GCGATTGCAA 60

GAGATTCGAG AGGGGCTGAA GCAGCCATCC AAGGCTTCCA CCCAGGGGCT GCTGGTGGGA 120

CCAAACAGTG ACACTTCCCT GGATGCCAAA GTCCTGGGGA GCAAAGATGC CTCCAGGCAG 180

CAGCAAATGA GAGCCACCCC GAAGTTTGGA CCTTATCAAA AAGCTCTCAG GGAAATCCGA 240

TATTCCCTCC TGCCTTTTGC CAACGAGTCA GGCACTTCGG CAGCTGCAGA GGTGAACCGG 300

CAGATGCTTC AGGAGTTGGT GAATGCGGGA TGTGACCAGG AGATGGCTGG CAGAGCGCTC 360

AAGCAGACGG GCAGTAGGAG TATCGAAGCT GCCTTGGAGT ACATCAGTAA GATGGGCTAC 420

CTGGACCCCA GGAATGAGCA GATTGTGCGA GTCATCAAGC AGACCTCCCC AGGAAAGGGC 480

CTGGCGCCCA CCCCGGTGAC TCGGCGGCCC AGTTTCGAGG GCACAGGGGA AGCACTCCCA 540

TCCTACCACC AGCTGGGTGG TGCAAACTAC GAGGGCCCCG CCGCACTGGA GGAGATGCCG 600

CGGCAATATT TAGACTTTCT CTTCCCTGGA GCCGGAGCCG GCACCCACGG TGCCCAGGCT 660

CACCAGCATC CTCCCAAAGG GTACAGCACA GCAGTAGAGC CAAGTGCGCA CTTTCCGGGC 720

ACACACTATG GTCGTGGTCA TCTACTATCG GAGCAGCCTG GGTATGGGGT GCAGCGCAGT 780

TCCTCCTTCC AGAACAAGAC GCCACCAGAT GCCTATTCCA GCATGGCCAA GGCCCAGGGT 840

GGCCCTCCCG CCAGCCTCAC CTTTCCTGCC CATGCTGGGC TGTACACTGC CTCGCACCAC 900

AAGCCGGCGG CTACCCCACC TGGGGCCCAC CCATTACATG TGTTGGGCAC CCGGGGTCCC 960

ACGTTTACTG GCGAAAGCTC TGCACAGGCT GTGCTGGCAC CGTCCAGGAA CAGCCTCAAT 1020

GCTGACTTGT ACGAGCTGGG CTCCACGGTG CCCTGGTCTG CAGCTCCACT GGCACGCCGC 1080

GACTCGCTGC AGAAGCAGGG TCTAGAAGCC TCGCGGCCGC ATGTGGCTTT TCGGGCTGGC 1140

CCCAGCAGGA CCAACTCCTT CAACAACCCA CAACCTGAGC CCTCACTGCC CGCCCCCAAC 1200

ACGGTCACCG CCGTGACGGC CGCACACATC CTTCACCCTG TGAAGAGCGT GCGTGTGCTG 1260

CGGCCCGAGC CCCAGACAGC CGTGGGGCCC TCGCACCCCG CCTGGGTGGC TGCGCCCACA 1320

GCACCTGCCA CTGAGAGCCT GGAGACGAAG GAGGGCAGCG CAGGCCCACA CCCGCTGGAT 1380

GTGGACTATG GCGGCTCCGA GCGCAGGTGC CCACCGCCTC CGTACCCAAA GCACTTGCTG 1440

CTGCCCAGTA AGTCTGAGCA GTACAGCGTG GACCTGGACA GCCTGTGCAC CAGTGTGCAG 1500

CAGAGTCTGC GAGGGGGCAC TGAGCAAGAC AGGAGTGACA AGAGCCACAA AGGTGCGAAG 1560

GGAGACAAAG CTGGCAGAGA CAAAAAGCAG ATTCAGACCT CCCCGGTGCC TGTCCGCAAG 1620

AATAGCAGAG ATGAAGAGAA GAGAGAGTCT CGCATCAAGA GTTACTCCCC TTATGCCTTC 1680

AAATTCTTCA TGGAGCAACA CGTGGAGAAT GTCATCAAAA CCTACCAGCA GAAGGTCAGC 1740

CGGAGGCTAC AGCTGGAGCA GGAAATGGCC AAAGCTGGGC TCTGTGAGGC CGAGCAGGAG 1800

CAGATGAGGA AGATCCTCTA CCAGAAGGAG TCTAACTACA ACCGGCTGAA GAGGGCCAAG 1860

ATGGACAAGT CCATGTTTGT GAAAATCAAG ACTCTAGGCA TCGGTGCCTT TGGGGAAGTG 1920

TGCCTCGCTT GTAAGCTGGA CACTCACGCT CTGTACGCCA TGAAGACTCT CAGGAAGAAG 1980

GATGTCCTGA ACCGGAATCA AGTGGCCCAT GTCAAGGCTG AGAGGGACAT CCTGGCTGAA 2040

GCAGACAATG AGTGGGTGGT CAAACTCTAC TACTCCTTCC AGGACAAGGA CAGCCTGTAC 2100

TTTGTGATGG ACTACATACC AGGCGGGGAT ATGATGAGCC TGCTGATCAG GATGGAGGTC 2160

TTCCCTGAGC ACCTGGCCCG CTTCTACATT GCAGAGTTGA CCCTGGCCAT TGAAAGTGTC 2220

CACAAGATGG GCTTTATCCA CCGGGACATC AAGCCTGACA ACATACTCAT CGACCTGGAT 2280

GGTCATATTA AGCTGACAGA TTTTGGCCTC TGCACTGGAT TCAGGTGGAC TCACAATTCC 2340

AAGTACTACC AGAAAGGGAA CCACATGAGA CAGGACAGCA TGGAGCCCGG TGACCTCTGG 2400

GACGATGTTT CCAACTGTCG CTGTGGAGAC AGGTTAAAGA CCCTGGAGCA GAGGGCGCAG 2460

AAGCAGCACC AGAGGTGCCT GGCACATTCT CTTGTCGGGA CACCAAATTA CATCGCTCCG 2520

GAGGTGCTTC TCCGCAAAGG GTACACGCAG CTCTGTGACT GGTGGAGCGT CGGTGTGATT 2580

CTCTTTGAGA TGCTGGTTGG GCAGCCGCCT TTCTTGGCCC CCACCCCCAC AGAGACGCAG 2640

CTGAAGGTGA TCAACTGGGA GAGCACGCTG CATATCCCTA CGCAGGTGAG GCTCAGCGCT 2700

GAGGCCCGAG ACCTCATCAC GAAGCTGTGC TGCGCGGCTG ACTGCCGCCT GGGCAGGGAT 2760

GGGGCAGATG ACCTCAAGGC ACACCCGTTC TTCAACACCA TCGACTTTTC CCGTGACATC 2820

CGAAAGCAGC CTGCACCCTA CGTCCCCACC ATCAGCCACC CCATGGACAC CTCCAATTTT 2880

GACCCGGTGG ATGAAGAAAG CCCCTGGCAC GAGGCCAGCG GAGAGAGCGC CAAGGCCTGG 2940

GACACGCTGG CCTCCCCCAG CAGCAAGCAT CCAGAGCACG CCTTCTATGA GTTCACCTTC 3000

CGCAGGTTCT TCGATGACAA CGGCTATCCC TTCCGGTGCC CGAAGCCCTC AGAGCCCGCA 3060

GAGAGTGCAG ACCCAGGGGA TGCGGACTTG GAAGGTGCGG CCGAGGGCTG CCAGCCGGTG 3120

TACGTGTAA 3129

Domain Profile

S: 1     feklkvigkGafGevrlvrkkdtkevyalkilrkkdllkknqvahvkaerdilaeadsew  60

         f k+k++g+GafGev+l++k dt+++ya+k+lrkkd+l++nqvahvkaerdilaead+ew

Q: 626   FVKIKTLGIGAFGEVCLACKLDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEW  685

         889*********************************************************

S: 61    vvklyysfqdkenlylvmeylPGGdlmtllikkeilkeelarfyiaelvlavesvhklGf  120

         vvklyysfqdk++ly+vm+y+PGGd+m+lli++e+++e+larfyiael+la+esvhk+Gf

Q: 686   VVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGF  745

         ************************************************************

S: 121   ihrdlkPdnllidakGhikltdfGlctGlkkthrseyykelekakeddlease.......  173

         ihrd+kPdn+lid +GhikltdfGlctG+++th+s+yy++ +++++d++e  +

Q: 746   IHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGNHMRQDSMEPGDlwddvsn  805

         *************************************************9999999****

S: 174   .....elkvlerrkkkknrrllayslvGtpdyiapevlekkgynklcdwWslGvilyeml  228

              +lk+le+r++k+++r+la+slvGtp+yiapevl++kgy++lcdwWs+Gvil+eml

Q: 806   crcgdRLKTLEQRAQKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEML  865

         ************************************************************

S: 229   vGypPflaetpqetykkvinwketlkfpeevelseeakdlierliadaekrlgkkGvdei  288

         vG+pPfla tp+et+ kvinw++tl++p++v+ls+ea+dli++l++ a+ rlg++G+d++

Q: 866   VGQPPFLAPTPTETQLKVINWESTLHIPTQVRLSAEARDLITKLCCAADCRLGRDGADDL  925

         ************************************************************

S: 289   kkhpff  294

         k+hpff

Q: 926   KAHPFF  931

         *****9

Domain Sequence
(FASTA)

FVKIKTLGIG AFGEVCLACK LDTHALYAMK TLRKKDVLNR NQVAHVKAER DILAEADNEW 60

VVKLYYSFQD KDSLYFVMDY IPGGDMMSLL IRMEVFPEHL ARFYIAELTL AIESVHKMGF 120

IHRDIKPDNI LIDLDGHIKL TDFGLCTGFR WTHNSKYYQK GNHMRQDSME PGDLWDDVSN 180

CRCGDRLKTL EQRAQKQHQR CLAHSLVGTP NYIAPEVLLR KGYTQLCDWW SVGVILFEML 240

VGQPPFLAPT PTETQLKVIN WESTLHIPTQ VRLSAEARDL ITKLCCAADC RLGRDGADDL 300

KAHPFF 306

Keyword

3D-structure; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005815	; C:microtubule organizing center
GO:0005634	; C:nucleus
GO:0000922	; C:spindle pole
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0034613	; P:cellular protein localization
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0035329	; P:hippo signaling cascade
GO:0009755	; P:hormone-mediated signaling pathway
GO:0007243	; P:intracellular protein kinase cascade
GO:0030216	; P:keratinocyte differentiation
GO:0007067	; P:mitosis
GO:0090090	; P:negative regulation of canonical Wnt receptor signaling pathway
GO:0045736	; P:negative regulation of cyclin-dependent protein kinase activity
GO:0046620	; P:regulation of organ growth

KEGG

mmu:50523

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR009060	; UBA-like.
IPR015940	; UBA/transl_elong_EF1B_N_euk.

Pfam

PF00069	; Pkinase; 2.
PF00433	; Pkinase_C; 1.

SMARTs

SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; 1.

Prints

Created Date

20-Feb-2013