| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00350 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| STE/STE20 | 346.3 | 2.8E-103 | 27 | 278 | 252 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000018476 |
| UniProt Accession | Q9JI10; Q60877; Q80UG4; Q8CI58; |
| Protein Name | Serine/threonine-protein kinase 3 20kDa subunit |
| Protein Synonyms/Alias | Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; MST2/N; MST2/C; |
| Gene Name | Stk3 |
| Gene Synonyms/Alias | Stk3; Mess1, Mst2; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Stress-activated, pro-apoptotic kinase which, followingcaspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (By similarity). |
| Protein Length | 497 |
Protein Sequence
(FASTA) | | MEQPPASKSK LKKLSEDSLT KQPEEVFDVL EKLGEGSYGS VFKAIHKESG QVVAIKQVPV 60 | | ESDLQEIIKE ISIMQQCDSP YVVKYYGSYF KNTDLWIVME YCGAGSVSDI IRLRNKTLTE 120 | | DEIATILKST LKGLEYLHFM RKIHRDIKAG NILLNTEGHA KLADFGVAGQ LTDTMAKRNT 180 | | VIGTPFWMAP EVIQEIGYNC VADIWSLGIT SIEMAEGKPP YADIHPMRAI FMIPTNPPPT 240 | | FRKPELWSDD FTDFVKKCLV KSPEQRATAT QLLQHPFIKN AKPVSILRDL IAEAMEIKAK 300 | | RHEEQQRELE EEEENSDEDE LDSHTMVKTS SESVGTMRAT STMSEGAQTM IEHNSTMLES 360 | | DLGTMVINSE EEEEEEEEEE EDGTMKRNAT SPQVQRPSFM DYFDKQDFKN KSHENCDQSM 420 | | REPGPMSNSV FPDNWRVPQD GDFDFLKNLS LEELQMRLKA LDPMMEREIE ELHQRYSAKR 480 | | QPILDAMDAK KRRQQNF 497 |
|
Nucleotide Sequence
(FASTA) | | ATGGAGCAGC CGCCGGCGTC CAAGAGTAAG CTAAAAAAGC TGAGTGAAGA CAGTTTGACT 60 | | AAGCAGCCTG AAGAAGTTTT TGATGTACTG GAGAAGCTTG GAGAAGGGTC TTATGGAAGT 120 | | GTTTTTAAAG CAATACATAA GGAATCTGGT CAAGTGGTTG CAATTAAGCA AGTACCTGTT 180 | | GAGTCAGATC TTCAGGAAAT AATCAAAGAA ATTTCCATAA TGCAACAATG TGACAGTCCA 240 | | TATGTTGTGA AGTACTATGG CAGTTACTTT AAGAACACAG ACCTCTGGAT TGTTATGGAG 300 | | TACTGTGGAG CGGGGTCCGT TTCAGACATA ATTAGATTGC GAAACAAGAC ATTAACAGAA 360 | | GATGAAATTG CAACTATTCT AAAATCCACA TTGAAAGGAT TAGAATATTT GCATTTTATG 420 | | AGGAAAATAC ACAGAGATAT AAAAGCCGGG AATATTCTCC TCAATACAGA AGGACATGCA 480 | | AAGCTTGCAG ATTTTGGAGT GGCTGGCCAG TTAACAGATA CAATGGCAAA ACGCAACACT 540 | | GTAATAGGAA CCCCATTTTG GATGGCTCCT GAGGTAATTC AAGAAATAGG TTACAACTGT 600 | | GTGGCTGACA TCTGGTCCCT TGGCATTACT TCTATAGAAA TGGCAGAAGG AAAACCTCCT 660 | | TATGCTGATA TACATCCGAT GAGGGCTATT TTTATGATCC CTACAAACCC ACCACCAACA 720 | | TTCAGGAAAC CTGAACTTTG GTCTGATGAC TTCACCGATT TTGTGAAGAA GTGCTTGGTG 780 | | AAGAGTCCTG AGCAGAGAGC CACTGCGACA CAGCTGTTAC AGCATCCTTT TATCAAGAAT 840 | | GCAAAACCCG TGTCGATATT AAGAGACCTG ATCGCAGAAG CCATGGAAAT CAAAGCCAAA 900 | | CGGCATGAAG AGCAGCAGCG GGAGCTAGAG GAGGAGGAAG AAAACTCGGA TGAAGATGAG 960 | | CTGGATTCAC ACACTATGGT GAAGACGAGT TCAGAAAGCG TGGGCACAAT GCGGGCCACC 1020 | | AGCACGATGA GTGAAGGAGC CCAGACGATG ATTGAACATA ACAGCACCAT GTTAGAGTCG 1080 | | GACCTGGGGA CCATGGTTAT AAACAGTGAA GAAGAGGAGG AAGAGGAAGA GGAGGAGGAG 1140 | | GAGGACGGAA CTATGAAAAG AAATGCAACG TCACCTCAAG TACAAAGACC TTCTTTCATG 1200 | | GACTACTTTG ATAAGCAGGA CTTCAAGAAC AAGAGTCATG AAAATTGTGA TCAGAGCATG 1260 | | CGTGAGCCAG GCCCTATGTC CAACAGTGTT TTTCCTGACA ACTGGAGAGT TCCTCAAGAT 1320 | | GGAGACTTTG ATTTCTTAAA AAATCTAAGT TTAGAAGAAC TACAGATGCG GCTAAAAGCA 1380 | | CTAGACCCCA TGATGGAACG AGAAATAGAA GAACTGCATC AAAGATACAG TGCAAAAAGA 1440 | | CAACCTATCC TGGATGCCAT GGATGCGAAG AAGAGGAGGC AGCAGAATTT CTGA 1494 |
|
| Domain Profile | S: 1 yelleeigkGalgkvylarekktgelvaikkinlekqekleelqkeisvlkklkhpnive 60 | +++le++g+G++g+v++a +k++g++vaik++ +e+ +l+e++keis+++++++p++v+ | Q: 27 FDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVES--DLQEIIKEISIMQQCDSPYVVK 84 | 7899*******************************7..9********************* |
| S: 61 ylesflkeeelwlvmeylagGslsdlvkeelkselkEeqiayilrevlkaLeylHskkvi 120 | y++s++k+++lw+vmey+ +Gs+sd+++ ++++l+E++ia+il+++lk+LeylH +++i | Q: 85 YYGSYFKNTDLWIVMEYCGAGSVSDIIR-LRNKTLTEDEIATILKSTLKGLEYLHFMRKI 143 | ****************************.99***************************** |
| S: 121 HrdikakniLlteegeVkLsdfgvaaqlektrkkrktvvgtpywmAPEviaqeekgYdek 180 | Hrdika+niLl++eg+ kL+dfgva ql++t++kr+tv+gtp+wmAPEvi+ e gY++ | Q: 144 HRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQ--EIGYNCV 201 | ***************************************************..9****** |
| S: 181 aDiwslGItaielaegepPfkdleplkallkilkneppklkskkkfskefkefvekclqk 240 | aDiwslGIt+ie+aeg+pP++d++p++a+++i++n+pp++++++ +s+ f++fv+kcl k | Q: 202 ADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVK 261 | ************************************************************ |
| S: 241 npekRpsasellkhkFl 257 | +pe+R++a++ll+h+F+ | Q: 262 SPEQRATATQLLQHPFI 278 | ****************7 |
|
Domain Sequence
(FASTA) | | FDVLEKLGEG SYGSVFKAIH KESGQVVAIK QVPVESDLQE IIKEISIMQQ CDSPYVVKYY 60 | | GSYFKNTDLW IVMEYCGAGS VSDIIRLRNK TLTEDEIATI LKSTLKGLEY LHFMRKIHRD 120 | | IKAGNILLNT EGHAKLADFG VAGQLTDTMA KRNTVIGTPF WMAPEVIQEI GYNCVADIWS 180 | | LGITSIEMAE GKPPYADIHP MRAIFMIPTN PPPTFRKPEL WSDDFTDFVK KCLVKSPEQR 240 | | ATATQLLQHP FI 252 |
|
| Keyword | Acetylation; Alternative splicing; Apoptosis; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |