EKS-MUM-00348

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00348

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE20	348.6	5.5E-104	249	500	252

Status

Reviewed

Ensembl Protein

ENSMUSP00000023467

UniProt Accession

Q8CIN4;

Protein Name

PAK-2p34

Protein Synonyms/Alias

Gamma-PAK; p21-activated kinase 2; PAK-2;

Gene Name

Pak2

Gene Synonyms/Alias

Pak2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000022781	ENSMUSP00000023467	ENSMUST00000023467

Organism

Mus musculus

Functional Description

Serine/threonine protein kinase that plays a role in avariety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF- induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase- activated PAK2 phosphorylates MKNK1 and reduces cellular translation (By similarity).

Protein Length

524

Protein Sequence
(FASTA)

MSDNGELEDK PPAPPVRMSS TIFSTGGKDP LSANHSLKPL PSVPEEKKPR NKIISIFSGT 60

EKGSKKKEKE RPEISPPSDF EHTIHVGFDA VTGEFTGMPE QWARLLQTSN ITKLEQKKNP 120

QAVLDVLKFY DSNTVKQKYL SFTPPEKDGF PSGTPALNTK GSETSAVVTE EDDDDEDAAP 180

PVIAPRPDHT KSIYTRSVID PIPAPVGDSN VDSGAKSSDK QKKKAKMTDE EIMEKLRTIV 240

SIGDPKKKYT RYEKIGQGAS GTVFTATDVA LGQEVAIKQI NLQKQPKKEL IINEILVMKE 300

LKNPNIVNFL DSYLVGDELF VVMEYLAGGS LTDVVTETCM DEAQIAAVCR ECLQALEFLH 360

ANQVIHRDIK SDNVLLGMEG SVKLTDFGFC AQITPEQSKR STMVGTPYWM APEVVTRKAY 420

GPKVDIWSLG IMAIEMVEGE PPYLNENPLR ALYLIATNGT PELQNPEKLS PIFRDFLNRC 480

LEMDVEKRGS AKELLQHPFL KLAKPLSSLT PLILAAKEAM KSNR 524

Nucleotide Sequence
(FASTA)

ATGTCTGATA ACGGAGAGCT AGAAGACAAG CCCCCAGCAC CTCCAGTTCG GATGAGCAGT 60

ACCATTTTTA GCACCGGAGG AAAAGATCCT TTATCAGCCA ATCACAGTTT GAAACCTTTG 120

CCTTCTGTTC CAGAGGAAAA AAAACCCAGG AACAAAATCA TCTCCATATT CTCTGGCACA 180

GAAAAAGGAA GTAAAAAGAA AGAAAAAGAA CGGCCAGAGA TTTCTCCCCC ATCTGATTTT 240

GAGCACACCA TCCATGTTGG CTTTGATGCT GTTACGGGAG AGTTCACTGG CATGCCAGAA 300

CAGTGGGCGC GGCTGTTGCA GACCTCCAAC ATTACCAAAC TCGAGCAGAA GAAGAACCCT 360

CAGGCAGTGC TGGATGTCTT GAAGTTCTAC GACTCCAACA CTGTGAAACA GAAGTACCTG 420

AGTTTCACTC CTCCTGAGAA AGATGGCTTC CCTTCTGGAA CACCAGCACT GAACACCAAG 480

GGGTCAGAGA CATCAGCTGT AGTGACAGAG GAAGATGATG ATGATGAAGA CGCTGCTCCT 540

CCCGTCATTG CCCCTCGGCC AGATCATACA AAATCAATTT ACACACGGTC TGTCATCGAC 600

CCCATTCCTG CTCCAGTTGG TGATTCTAAT GTTGACAGTG GTGCCAAGTC TTCAGACAAA 660

CAGAAAAAGA AAGCCAAGAT GACCGATGAA GAGATTATGG AGAAATTAAG AACTATTGTG 720

AGCATAGGGG ACCCAAAGAA AAAATACACA AGATATGAAA AAATTGGGCA AGGGGCTTCT 780

GGAACAGTTT TTACTGCCAC TGATGTGGCC CTGGGGCAAG AGGTTGCTAT CAAGCAGATT 840

AATTTACAGA AACAACCAAA GAAGGAATTG ATCATTAATG AAATTCTGGT GATGAAAGAG 900

TTAAAGAATC CCAACATAGT TAACTTCTTG GACAGTTACC TGGTAGGAGA TGAGTTGTTT 960

GTGGTAATGG AGTACCTCGC TGGTGGGTCC CTCACTGATG TTGTAACAGA AACCTGCATG 1020

GACGAAGCGC AGATTGCCGC CGTGTGCAGA GAGTGTTTAC AGGCGTTGGA GTTTTTACAT 1080

GCTAATCAAG TGATCCACAG AGACATCAAA AGTGACAATG TGCTTTTGGG AATGGAAGGC 1140

TCAGTTAAAC TTACTGACTT CGGCTTCTGT GCCCAGATCA CTCCTGAACA GAGCAAACGC 1200

AGTACTATGG TTGGAACACC GTACTGGATG GCACCAGAGG TGGTGACACG GAAAGCCTAT 1260

GGTCCCAAAG TTGACATATG GTCTCTGGGC ATCATGGCTA TCGAGATGGT TGAAGGAGAG 1320

CCTCCATACC TCAACGAAAA TCCTCTGCGG GCATTATACC TGATAGCTAC AAATGGAACT 1380

CCTGAACTTC AGAATCCAGA AAAACTTTCC CCAATATTTC GGGATTTCTT AAATCGGTGT 1440

TTGGAAATGG ATGTGGAGAA AAGGGGTTCG GCCAAGGAAC TGTTACAGCA TCCTTTCCTG 1500

AAACTGGCCA AACCATTGTC TAGCTTGACG CCACTGATCC TGGCAGCTAA AGAAGCAATG 1560

AAGAGTAACC GCTAA 1575

Domain Profile

S: 1     yelleeigkGalgkvylarekktgelvaikkinlekqekleelqkeisvlkklkhpnive  60

         y+ +e+ig+Ga+g+v++a+++  g+ vaik+inl+kq+k+e++++ei v+k+lk+pniv+

Q: 249   YTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVN  308

         7889********************************************************

S: 61    ylesflkeeelwlvmeylagGslsdlvkeelkselkEeqiayilrevlkaLeylHskkvi  120

         +l+s+l+ +el++vmeylagGsl+d+v+   ++ ++E qia+++re+l+aLe+lH+++vi

Q: 309   FLDSYLVGDELFVVMEYLAGGSLTDVVT---ETCMDEAQIAAVCRECLQALEFLHANQVI  365

         ****************************...9****************************

S: 121   HrdikakniLlteegeVkLsdfgvaaqlektrkkrktvvgtpywmAPEviaqeekgYdek  180

         Hrdik++n+Ll +eg+VkL+dfg++aq++ +++kr+t+vgtpywmAPEv++  +k Y  k

Q: 366   HRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVT--RKAYGPK  423

         ***************************************************..9******

S: 181   aDiwslGItaielaegepPfkdleplkallkilkneppklkskkkfskefkefvekclqk  240

         +DiwslGI+aie++egepP+ +++pl+al++i++n +p+l++++k+s+ f++f+++cl+

Q: 424   VDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEM  483

         ************************************************************

S: 241   npekRpsasellkhkFl  257

         ++ekR sa+ell+h+Fl

Q: 484   DVEKRGSAKELLQHPFL  500

         ****************7

Domain Sequence
(FASTA)

YTRYEKIGQG ASGTVFTATD VALGQEVAIK QINLQKQPKK ELIINEILVM KELKNPNIVN 60

FLDSYLVGDE LFVVMEYLAG GSLTDVVTET CMDEAQIAAV CRECLQALEF LHANQVIHRD 120

IKSDNVLLGM EGSVKLTDFG FCAQITPEQS KRSTMVGTPY WMAPEVVTRK AYGPKVDIWS 180

LGIMAIEMVE GEPPYLNENP LRALYLIATN GTPELQNPEK LSPIFRDFLN RCLEMDVEKR 240

GSAKELLQHP FL 252

Keyword

Acetylation; Allosteric enzyme; Apoptosis; ATP-binding; Complete proteome; Cytoplasm; Growth regulation; Kinase; Lipoprotein; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0048471	; C:perinuclear region of cytoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030296	; F:protein tyrosine kinase activator activity
GO:0043066	; P:negative regulation of apoptotic process
GO:2001271	; P:negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
GO:0018105	; P:peptidyl-serine phosphorylation
GO:2001238	; P:positive regulation of extrinsic apoptotic signaling pathway
GO:0046777	; P:protein autophosphorylation
GO:0040008	; P:regulation of growth

KEGG

mmu:224105

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000095	; PAK_box_Rho-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00786	; PBD; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00285	; PBD; 1.
SM00220	; S_TKc; 1.

Prosites

PS50108	; CRIB; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013