EKS-MUM-00414

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00414

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	316.1	4.3E-94	161	396	236

Status

Reviewed

Ensembl Protein

ENSMUSP00000023812

UniProt Accession

Q60700; P70286; Q3TLL7; Q8C4N7; Q8CBX3; Q8CDL6;

Protein Name

Mitogen-activated protein kinase kinase kinase 12

Protein Synonyms/Alias

Dual leucine zipper bearing kinase; DLK; Leucine-zipper protein kinase; ZPK; MAPK-upstream kinase; MUK; Mixed lineage kinase;

Gene Name

Map3k12

Gene Synonyms/Alias

Map3k12; Zpk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000023050	ENSMUSP00000133209	ENSMUST00000169377
ENSMUSG00000023050	ENSMUSP00000129251	ENSMUST00000169162
ENSMUSG00000023050	ENSMUSP00000130786	ENSMUST00000165174
ENSMUSG00000023050	ENSMUSP00000023812	ENSMUST00000023812
ENSMUSG00000023050	ENSMUSP00000127629	ENSMUST00000171565
ENSMUSG00000023050	ENSMUSP00000129472	ENSMUST00000169367

Organism

Mus musculus

Functional Description

May be an activator of the JNK/SAPK pathway.Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Protein Length

888

Protein Sequence
(FASTA)

MACLHETRTP SPSFGGFVST LSEASMRKLD PDTSDCTPEK DLTPTQCVLR DVVPLGGQGG 60

GGPSPSPGGE PPPEPFANSV LQLHEQDTGG PGGATGSPES RASRVRADEV RLQCQSGSGF 120

LEGLFGCLRP VWTMIGKAYS TEHKQQQEDL WEVPFEEILD LQWVGSGAQG AVFLGRFHGE 180

EVAVKKVRDL KETDIKHLRK LKHPNIITFK GVCTQAPCYC ILMEFCAQGQ LYEVLRAGRP 240

VTPSLLVDWS MGIAGGMNYL HLHKIIHRDL KSPNMLITYD DVVKISDFGT SKELSDKSTK 300

MSFAGTVAWM APEVIRNEPV SEKVDIWSFG VVLWELLTGE IPYKDVDSSA IIWGVGSNSL 360

HLPVPSSCPD GFKILLRQCW NSKPRNRPSF RQILLHLDIA SADVLSTPQE TYFKSQAEWR 420

EEVKLHFEKI KSEGTCLHRL EEELVMRRRE ELRHALDIRE HYERKLERAN NLYMELNALM 480

LQLELKEREL LRREQALERR CPGLLKSHPS RGLLHGNTME KLIKKRNVPQ KLSPHSKRPD 540

ILKTESLLPK LDAALSGVGL PGCPKGPPSP GRSRRGKTRH RKASAKGSCG DLPGLRAALP 600

PHEPGGLGSP GGLGVGPSAW DACPPALRGL HHDLLLRKMS SSSPDLLSAA LGARGRGATG 660

GARDPGSPPP PQGDTPPSEG SAPGSTSPDS PGGAKGEPPP PVGPGEGVGL LGTGREGTAG 720

RGGNRAGSQH LTPAALLYRA AVTRSQKRGI SSEEEEGEVD SEVELPPSQR WPQGPNMRQS 780

LSTFSSENPS DVEEGTASEP SPSGTPEVGS TNTDERPDER SDDMCSQGSE IPLDLPTSEV 840

VPEREASSLP MQHQDGQGPN PEDSDCDSTE LDNSNSIDAL RPPASLPP 888

Nucleotide Sequence
(FASTA)

ATGGCCTGCC TCCATGAAAC CCGAACACCC TCCCCTTCCT TTGGGGGCTT TGTGTCTACT 60

CTAAGTGAGG CTTCTATGCG AAAGCTGGAT CCAGACACTT CAGACTGCAC CCCCGAGAAA 120

GACCTGACGC CCACCCAATG TGTACTTCGA GATGTTGTGC CTCTCGGTGG GCAGGGTGGA 180

GGAGGACCCA GCCCCTCCCC AGGTGGAGAG CCTCCCCCAG AGCCTTTTGC CAATAGTGTC 240

CTCCAGCTAC ATGAGCAGGA TACAGGTGGG CCAGGGGGAG CCACTGGGTC ACCTGAGAGT 300

CGAGCATCTA GAGTTCGAGC TGATGAGGTA CGTCTGCAGT GCCAGAGTGG CAGTGGCTTC 360

CTTGAAGGTC TTTTTGGCTG CCTGCGCCCT GTCTGGACAA TGATTGGCAA AGCCTACTCC 420

ACAGAACACA AGCAACAGCA GGAAGACCTT TGGGAAGTCC CCTTTGAGGA AATCCTGGAC 480

CTGCAGTGGG TAGGCTCAGG GGCTCAGGGT GCTGTTTTCC TGGGACGCTT CCACGGGGAG 540

GAGGTAGCTG TGAAGAAGGT TCGAGATCTC AAGGAGACTG ACATCAAGCA TCTTCGAAAG 600

CTGAAGCACC CCAACATCAT CACTTTCAAG GGTGTTTGCA CACAGGCCCC CTGCTACTGC 660

ATCCTTATGG AATTCTGCGC TCAAGGACAG CTATATGAGG TGCTCAGAGC CGGCCGCCCT 720

GTCACCCCCT CCTTGCTGGT TGACTGGTCC ATGGGCATCG CTGGTGGCAT GAATTACCTG 780

CACCTGCACA AGATTATCCA CAGAGACCTG AAGTCACCCA ACATGCTAAT CACATACGAC 840

GATGTGGTGA AGATCTCAGA TTTTGGCACT TCCAAGGAGC TGAGTGACAA GAGCACCAAG 900

ATGTCCTTTG CAGGAACAGT AGCCTGGATG GCTCCTGAAG TGATCAGAAA TGAACCTGTG 960

TCTGAGAAGG TTGACATCTG GTCCTTTGGG GTGGTGCTAT GGGAACTACT GACTGGAGAG 1020

ATTCCCTACA AAGATGTAGA TTCCTCAGCC ATCATCTGGG GTGTGGGAAG CAACAGTCTC 1080

CACCTGCCTG TACCCTCCAG CTGCCCAGAT GGTTTTAAAA TTCTACTTCG CCAGTGCTGG 1140

AACAGCAAAC CACGAAATCG CCCATCATTC CGACAGATCT TGCTGCACCT GGACATCGCC 1200

TCCGCTGATG TGCTCTCTAC ACCCCAGGAG ACTTACTTTA AGTCCCAGGC AGAGTGGCGG 1260

GAAGAAGTAA AACTGCACTT TGAAAAGATT AAGTCAGAAG GGACCTGTCT GCACCGCCTA 1320

GAAGAGGAAC TGGTGATGCG GAGAAGGGAG GAGCTCAGAC ATGCCCTGGA CATCAGGGAG 1380

CACTATGAAC GGAAGTTGGA GAGAGCCAAC AACCTGTACA TGGAACTGAA TGCCCTCATG 1440

CTGCAACTAG AACTCAAAGA GAGGGAATTG CTCAGGCGAG AGCAGGCTTT AGAAAGGCGG 1500

TGTCCTGGTC TACTAAAGTC ACACCCTTCT CGGGGCCTCC TACATGGAAA CACTATGGAG 1560

AAGCTCATCA AGAAAAGGAA CGTGCCACAG AAACTGTCGC CCCACAGCAA AAGGCCAGAT 1620

ATTCTCAAGA CAGAGTCGTT GCTACCTAAA CTAGATGCAG CCCTAAGTGG GGTGGGGCTT 1680

CCTGGGTGTC CTAAGGGCCC CCCTTCACCT GGAAGGAGTC GGCGTGGCAA GACCCGTCAC 1740

CGAAAGGCCA GTGCCAAGGG CAGCTGTGGA GACCTGCCTG GGCTTCGTGC AGCTTTGCCA 1800

CCCCATGAGC CTGGAGGACT AGGAAGCCCA GGGGGCCTAG GAGTGGGCCC TTCAGCTTGG 1860

GATGCTTGCC CCCCTGCTCT CCGTGGACTC CACCATGACC TTCTACTCCG AAAGATGTCA 1920

TCATCATCCC CAGACCTGCT ATCAGCAGCG CTGGGAGCCC GAGGCCGAGG GGCTACAGGG 1980

GGAGCTCGGG ATCCTGGCTC ACCACCTCCA CCCCAGGGCG ATACTCCTCC AAGTGAGGGA 2040

TCAGCTCCTG GTTCCACCAG CCCAGATTCG CCTGGGGGAG CTAAAGGGGA ACCCCCTCCA 2100

CCAGTAGGGC CTGGAGAAGG TGTGGGGCTG CTGGGAACTG GAAGGGAAGG GACTGCAGGC 2160

CGGGGAGGAA ACCGGGCTGG GTCCCAGCAC TTGACCCCCG CTGCGCTGCT GTACAGGGCT 2220

GCTGTGACTC GAAGTCAGAA ACGTGGTATC TCATCTGAAG AAGAAGAAGG AGAGGTAGAC 2280

AGTGAAGTAG AGCTACCCCC AAGTCAAAGG TGGCCTCAGG GCCCGAACAT GCGTCAGTCA 2340

CTATCTACAT TCAGCTCAGA GAACCCATCA GATGTGGAGG AAGGTACAGC TAGTGAGCCT 2400

TCCCCAAGTG GCACACCAGA AGTTGGCAGT ACCAACACTG ATGAGCGACC AGATGAACGA 2460

TCTGATGACA TGTGCTCACA GGGCTCAGAA ATTCCACTGG ACCTACCTAC TTCAGAGGTG 2520

GTCCCTGAAC GTGAAGCCAG CTCCTTGCCC ATGCAACACC AGGATGGCCA GGGCCCCAAT 2580

CCTGAAGACT CAGACTGTGA CAGCACTGAA TTGGACAACT CCAACAGCAT TGATGCCTTG 2640

CGGCCCCCAG CCTCCCTTCC TCCATGA 2667

Domain Profile

S: 4     eeligsgafgkvykakwrkkevavKklreeaeekekleklereakllsklshpniiellG  63

          +++gsga+g+v+ ++++++evavKk+r+ +e+         ++k+l+kl+hpnii+++G

Q: 161   LQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKET---------DIKHLRKLKHPNIITFKG  211

         689**************************9999.........******************

S: 64    vcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseapkkii  123

         vc++ p+++++me++++g+L++vl++   r ++++ lv+w++ iA Gm+YLh +   kii

Q: 212   VCTQAPCYCILMEFCAQGQLYEVLRA--GRPVTPSLLVDWSMGIAGGMNYLHLH---KII  266

         **************************..99************************...9**

S: 124   hrdLksknvLlseekktlkicdFGtarelsk.stkmsaagtlawmaPEvlkeekvseksD  182

         hrdLks+n+L+ + ++++ki+dFGt++els+ stkms+agt+awmaPEv+++e+vsek+D

Q: 267   HRDLKSPNMLI-TYDDVVKISDFGTSKELSDkSTKMSFAGTVAWMAPEVIRNEPVSEKVD  325

         ***********.99****************99****************************

S: 183   vwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkLmkecwnedpe  242

         +wS+gvvLWellt+e+Pykd+++ ai+++v++++l+lp+psscp+ +k L+++cwn++p+

Q: 326   IWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPR  385

         ************************************************************

S: 243   kRpsfseivks  253

         +Rpsf++i+ +

Q: 386   NRPSFRQILLH  396

         ********876

Domain Sequence
(FASTA)

LQWVGSGAQG AVFLGRFHGE EVAVKKVRDL KETDIKHLRK LKHPNIITFK GVCTQAPCYC 60

ILMEFCAQGQ LYEVLRAGRP VTPSLLVDWS MGIAGGMNYL HLHKIIHRDL KSPNMLITYD 120

DVVKISDFGT SKELSDKSTK MSFAGTVAWM APEVIRNEPV SEKVDIWSFG VVLWELLTGE 180

IPYKDVDSSA IIWGVGSNSL HLPVPSSCPD GFKILLRQCW NSKPRNRPSF RQILLH 236

Keyword

ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0033267	; C:axon part
GO:0005829	; C:cytosol
GO:0030426	; C:growth cone
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0042803	; F:protein homodimerization activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0000186	; P:activation of MAPKK activity
GO:0016572	; P:histone phosphorylation
GO:0000165	; P:MAPK cascade
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0018107	; P:peptidyl-threonine phosphorylation
GO:0046777	; P:protein autophosphorylation

KEGG

mmu:26404

;

InterPros

IPR011009	; Kinase-like_dom.
IPR017419	; MAP3K12_MAP3K13.
IPR000719	; Prot_kinase_cat_dom.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF07714

; Pkinase_Tyr; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013