EKS-MUM-00460

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00460

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	336.2	2.9E-100	101	349	249

Status

Reviewed

Ensembl Protein

ENSMUSP00000024701

UniProt Accession

Q9ESG9; Q3TNN2; Q8R3L4;

Protein Name

Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

Protein Synonyms/Alias

Myt1 kinase;

Gene Name

Pkmyt1

Gene Synonyms/Alias

Pkmyt1; Myt1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000023908	ENSMUSP00000024701	ENSMUST00000024701

Organism

Mus musculus

Functional Description

Acts as a negative regulator of entry into mitosis (G2to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).

Protein Length

490

Protein Sequence
(FASTA)

MTMPTEGTPP PLSGTPIPVP AYFRHAEPGF SLKRPGGLSR SLPPRPPAKG CIPVSRLFPP 60

RTPGWHQPQP RRVSFLCETS EPLQSPGYDP SRPESFFQQN FQRLSRLGHG SYGEVFKVRS 120

KEDGRLYAVK RYMSPFRGPK DRTRKLAEVG GHEKVGQHPH CVRLERAWEE GGILYLQTEL 180

CGPSLQQHCE AWGASLPEAQ VWGYLRDILL ALDHLHSQGL VHLDVKPANI FLGPRGRCKL 240

GDFGLLVELG SAGAGEAQEG DPRYMAPELL QGSYGTAADV FSLGLTILEV ACNMELPHGG 300

EGWQQLRQGY LPPEFTAGLS SELRSVLAMM LEPDPQLRAT AEALLALPML RQPRPWNVLW 360

YMAAEALSRG WALWQALVTL LCWLWHGLVH PASWLQPPGP PATPPGSPPC SPLLDSTLSS 420

SWDNDSIGPS LSPETVLSRI TRRTSTPRGR YIPRDALDLT DVDSEPPRGP CPTFEPRNLL 480

SLFEDSLDPA 490

Nucleotide Sequence
(FASTA)

ATGACCATGC CCACCGAGGG CACCCCACCA CCCCTAAGTG GTACCCCCAT CCCAGTTCCA 60

GCTTACTTCC GACACGCAGA GCCTGGTTTC TCCCTCAAAA GGCCTGGGGG CCTCAGTCGG 120

AGCCTCCCAC CTCGGCCCCC TGCCAAGGGC TGCATCCCTG TCAGCCGTCT ATTCCCCCCT 180

CGCACCCCAG GCTGGCACCA GCCCCAGCCC CGGAGGGTGT CTTTCCTGTG TGAGACCTCA 240

GAGCCCCTGC AGAGTCCTGG GTATGACCCG AGCCGGCCCG AGTCCTTCTT TCAGCAGAAC 300

TTCCAGAGGC TCAGCCGCCT GGGTCATGGC TCATATGGAG AAGTCTTCAA GGTGCGCTCT 360

AAGGAAGATG GGCGACTCTA TGCTGTTAAG CGCTACATGT CGCCATTCCG CGGCCCCAAA 420

GACCGAACTC GTAAACTGGC TGAGGTAGGT GGCCATGAGA AAGTGGGGCA GCATCCACAC 480

TGCGTGAGAC TGGAGCGGGC CTGGGAGGAG GGTGGCATCC TATACCTGCA GACAGAACTC 540

TGCGGGCCCA GCCTGCAGCA ACACTGTGAA GCCTGGGGGG CCAGCCTGCC AGAGGCCCAG 600

GTCTGGGGCT ACTTGCGGGA CATTCTTCTG GCTCTGGACC ATCTACATAG TCAAGGCCTA 660

GTTCACCTTG ATGTCAAGCC TGCCAACATC TTCTTGGGCC CCCGGGGCCG CTGCAAGCTG 720

GGCGACTTTG GACTACTGGT GGAGCTGGGT TCAGCCGGTG CTGGCGAGGC CCAGGAGGGA 780

GATCCTCGCT ACATGGCCCC AGAACTGCTG CAGGGCTCTT ATGGGACAGC AGCAGATGTG 840

TTCAGTCTGG GTCTCACCAT CTTGGAAGTG GCCTGTAACA TGGAACTGCC CCATGGTGGG 900

GAGGGCTGGC AGCAGCTGCG CCAGGGATAC TTGCCCCCTG AGTTCACTGC TGGTCTGTCT 960

TCTGAGCTGC GTTCTGTCCT CGCCATGATG CTGGAGCCTG ACCCCCAGCT TCGAGCCACA 1020

GCTGAGGCCC TGTTGGCCTT ACCCATGCTG AGGCAGCCAC GTCCCTGGAA TGTTCTGTGG 1080

TATATGGCTG CGGAAGCCCT AAGTCGAGGC TGGGCCCTGT GGCAGGCCCT GGTCACTCTG 1140

CTCTGCTGGC TCTGGCACGG GCTGGTGCAT CCTGCCAGTT GGCTGCAGCC TCCAGGCCCA 1200

CCGGCCACAC CACCTGGCTC TCCACCTTGC AGCCCCCTCC TGGACAGCAC CCTCTCCAGC 1260

AGCTGGGATA ATGACAGCAT AGGTCCCTCA CTCTCCCCAG AGACCGTCCT GTCCCGGATC 1320

ACTAGAAGAA CCTCTACCCC TCGGGGCAGG TACATACCTA GGGATGCCCT GGACCTAACT 1380

GATGTGGACT CAGAGCCTCC AAGAGGTCCC TGCCCCACCT TTGAGCCAAG GAACCTCCTC 1440

AGCCTGTTTG AGGACTCCCT AGACCCAGCC TGA 1473

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikkskkplkglskekkalrEvtahavlgqhen  60

         F++++++G+G++gevfkv+++edg+lyA+k++++p++g ++++++l+Ev +h+++gqh++

Q: 101   FQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRYMSPFRGPKDRTRKLAEVGGHEKVGQHPH  160

         999*********************************************************

S: 61    vvryisaWeeddslyiqtElCeggsLeaalqeceakkkaleeaelkkilldlllglrylH  120

         +vr+ +aWee ++ly+qtElC g sL+++   cea+ ++l+ea+++ +l d+ll+l++lH

Q: 161   CVRLERAWEEGGILYLQTELC-GPSLQQH---CEAWGASLPEAQVWGYLRDILLALDHLH  216

         *********************.*******...****************************

S: 121   ssslvHlDikpenifitkevtlklgDlGlvtelnsedkskveEGDsrylakEilqeeysk  180

         s+ lvHlD+kp+nif+  ++++klgD+Gl++el+s+ + +++EGD ry+a+E+lq++y +

Q: 217   SQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGSAGAGEAQEGDPRYMAPELLQGSYGT  276

         ************************************************************

S: 181   lkaDiFslgltilevaanvelPsnGeaWhklRkgklpdeilkelsedlrellklmlepdp  240

         + aD+Fslgltileva+n+elP+ Ge W++lR+g+lp+e+++ ls++lr++l++mlepdp

Q: 277   A-ADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLAMMLEPDP  335

         *.**********************************************************

S: 241   erRptaeellkhev  254

         + R+tae+ll+ ++

Q: 336   QLRATAEALLALPM  349

         **********9876

Domain Sequence
(FASTA)

FQRLSRLGHG SYGEVFKVRS KEDGRLYAVK RYMSPFRGPK DRTRKLAEVG GHEKVGQHPH 60

CVRLERAWEE GGILYLQTEL CGPSLQQHCE AWGASLPEAQ VWGYLRDILL ALDHLHSQGL 120

VHLDVKPANI FLGPRGRCKL GDFGLLVELG SAGAGEAQEG DPRYMAPELL QGSYGTAADV 180

FSLGLTILEV ACNMELPHGG EGWQQLRQGY LPPEFTAGLS SELRSVLAMM LEPDPQLRAT 240

AEALLALPM 249

Keyword

ATP-binding; Cell cycle; Complete proteome; Endoplasmic reticulum; Golgi apparatus; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005789	; C:endoplasmic reticulum membrane
GO:0000139	; C:Golgi membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0010923	; P:negative regulation of phosphatase activity

KEGG

mmu:268930

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR016235	; Tyr/Thr_kinase_Cdc2_inhib.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013