| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00290 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| AGC/PDK1 | 423.9 | 7.3E-127 | 85 | 345 | 261 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000111066 |
| UniProt Accession | Q9Z2A0; A6H6U3; Q9R1D8; Q9R215; |
| Protein Name | 3-phosphoinositide-dependent protein kinase 1 |
| Protein Synonyms/Alias | mPDK1; |
| Gene Name | Pdpk1 |
| Gene Synonyms/Alias | Pdpk1; Pdk1; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Serine/threonine kinase which acts as a master kinase,phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF- kappa-B activation in macrophages. |
| Protein Length | 457 |
Protein Sequence
(FASTA) | | MARTTSQLYD AVPIQSSVVL CSCPSPSMVR SQTEPGSSPG IPSGVSRQGS TMDGTTAEAR 60 | | PSTNPLQQHP AQLPPQPRKK RPEDFKFGKI LGEGSFSTVV LARELATSRE YAIKILEKRH 120 | | IIKENKVPYV TRERDVMSRL DHPFFVKLYF TFQDDEKLYF GLSYAKNGEL LKYIRKIGSF 180 | | DETCTRFYTA EIVSALEYLH GKGIIHRDLK PENILLNEDM HIQITDFGTA KVLSPESKQA 240 | | RANSFVGTAQ YVSPELLTEK SACKSSDLWA LGCIIYQLVA GLPPFRAGNE YLIFQKIIKL 300 | | EYHFPEKFFP KARDLVEKLL VLDATKRLGC EEMEGYGPLK AHPFFETITW ENLHQQTPPK 360 | | LTAYLPAMSE DDEDCYGNYD NLLSQFGFMQ VSSSSSSHSL STVETSLPQR SGSNIEQYIH 420 | | DLDTNSFELD LQFSEDEKRL LLEKQAGGNP CLTGRTT 457 |
|
Nucleotide Sequence
(FASTA) | | ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCTGTGCCCA TTCAGTCCAG TGTGGTGCTA 60 | | TGTTCCTGCC CATCCCCATC AATGGTGAGG TCCCAGACTG AGCCCGGTTC GTCCCCTGGC 120 | | ATTCCTAGTG GTGTTAGCAG GCAGGGATCC ACCATGGATG GCACCACAGC TGAAGCCCGA 180 | | CCAAGCACCA ACCCCTTGCA GCAGCACCCT GCCCAGCTGC CACCACAGCC TCGCAAGAAA 240 | | CGCCCTGAAG ACTTCAAGTT TGGGAAAATT CTTGGCGAGG GCTCTTTTTC AACAGTTGTT 300 | | CTGGCCCGAG AACTGGCCAC TTCCAGAGAA TATGCTATTA AAATTCTGGA GAAACGTCAT 360 | | ATTATAAAAG AAAACAAAGT TCCGTATGTA ACTAGAGAGA GAGATGTGAT GTCACGCCTG 420 | | GATCACCCCT TCTTTGTGAA ACTTTATTTT ACATTTCAGG ACGACGAAAA GCTGTATTTT 480 | | GGCCTTAGTT ATGCCAAAAA TGGAGAGCTA CTTAAATACA TCCGCAAAAT TGGCTCATTT 540 | | GATGAGACCT GTACCCGGTT TTACACGGCT GAGATTGTGT CTGCTTTAGA GTACTTGCAT 600 | | GGCAAGGGCA TCATTCACAG AGACCTTAAA CCAGAAAACA TTTTGTTAAA TGAAGACATG 660 | | CACATCCAGA TCACAGATTT TGGAACAGCA AAAGTGTTAT CCCCAGAGAG CAAACAAGCC 720 | | AGGGCCAACT CATTTGTAGG AACAGCACAG TATGTTTCTC CAGAGCTGCT CACAGAGAAG 780 | | TCGGCGTGTA AAAGTTCAGA CCTTTGGGCC CTTGGATGTA TAATCTATCA GCTCGTGGCA 840 | | GGACTCCCAC CATTCAGAGC CGGGAATGAA TATCTTATAT TTCAGAAGAT CATTAAGCTG 900 | | GAATATCATT TCCCAGAAAA ATTCTTCCCT AAGGCTAGAG ATCTTGTGGA AAAACTCTTG 960 | | GTTTTAGATG CCACAAAGCG TTTAGGCTGT GAAGAGATGG AAGGGTACGG GCCTCTCAAA 1020 | | GCTCATCCAT TCTTTGAGAC CATCACTTGG GAGAATTTGC ACCAGCAGAC ACCTCCGAAG 1080 | | CTCACAGCTT ACCTACCAGC CATGTCAGAG GATGATGAAG ACTGCTATGG CAACTACGAC 1140 | | AATCTCCTGA GCCAGTTTGG CTTCATGCAG GTGTCATCCT CCTCCTCTTC CCACTCCCTG 1200 | | TCTACGGTGG AAACCAGCCT GCCCCAGAGG TCGGGCAGCA ACATAGAGCA GTACATCCAT 1260 | | GATTTGGACA CTAACTCTTT TGAACTAGAC TTACAGTTTT CAGAAGATGA AAAAAGGTTG 1320 | | TTATTGGAAA AGCAAGCCGG TGGAAACCCT TGCCTAACAG GACGTACTAC CTGA 1374 |
|
| Domain Profile | S: 1 fkfekelgeGsystvvlarekeskkeyaikvlekryiikekkvkyvtrekevlaklnehp 60 | fkf+k+lgeGs+stvvlare+++++eyaik+lekr+iike+kv+yvtre++v+++l +hp | Q: 85 FKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIKENKVPYVTRERDVMSRL-DHP 143 | 89******************************************************.9** |
| S: 61 givkLyatfqDeeslyfvleyakkGdllellkkvgsfdeactryyaaellsaleflhkkg 120 | ++vkLy+tfqD+e+lyf l+yak+G+ll++++k+gsfde+ctr+y+ae++sale+lh kg | Q: 144 FFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDETCTRFYTAEIVSALEYLHGKG 203 | ************************************************************ |
| S: 121 iihrDlkpeniLldkdgkilitDfGsakvlepklkedskkeeeesarsksfvGtaeyvsP 180 | iihrDlkpeniLl++d++i+itDfG+akvl+p++k+ ar++sfvGta+yvsP | Q: 204 IIHRDLKPENILLNEDMHIQITDFGTAKVLSPESKQ---------ARANSFVGTAQYVSP 254 | ******************************999999.........9************** |
| S: 181 elLtdsevskssDlWalgcilyqllaGkpPfraeneylifkkilkleyafpegfpekakd 240 | elLt+++++kssDlWalgci+yql+aG+pPfra neylif+ki+kley+fpe+f++ka+d | Q: 255 ELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYHFPEKFFPKARD 314 | ************************************************************ |
| S: 241 lveklLvkdakerltlee......ikahkff 265 | lveklLv da++rl++ee +kah+ff | Q: 315 LVEKLLVLDATKRLGCEEmegygpLKAHPFF 345 | ******************************9 |
|
Domain Sequence
(FASTA) | | FKFGKILGEG SFSTVVLARE LATSREYAIK ILEKRHIIKE NKVPYVTRER DVMSRLDHPF 60 | | FVKLYFTFQD DEKLYFGLSY AKNGELLKYI RKIGSFDETC TRFYTAEIVS ALEYLHGKGI 120 | | IHRDLKPENI LLNEDMHIQI TDFGTAKVLS PESKQARANS FVGTAQYVSP ELLTEKSACK 180 | | SSDLWALGCI IYQLVAGLPP FRAGNEYLIF QKIIKLEYHF PEKFFPKARD LVEKLLVLDA 240 | | TKRLGCEEME GYGPLKAHPF F 261 |
|
| Keyword | Acetylation; ATP-binding; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase; Ubl conjugation. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | | GO:0016023 | ; C:cytoplasmic membrane-bounded vesicle | | GO:0005829 | ; C:cytosol | | GO:0005925 | ; C:focal adhesion | | GO:0005634 | ; C:nucleus | | GO:0005886 | ; C:plasma membrane | | GO:0004676 | ; F:3-phosphoinositide-dependent protein kinase activity | | GO:0005524 | ; F:ATP binding | | GO:0032148 | ; P:activation of protein kinase B activity | | GO:0032869 | ; P:cellular response to insulin stimulus | | GO:0048041 | ; P:focal adhesion assembly | | GO:0006972 | ; P:hyperosmotic response | | GO:0035556 | ; P:intracellular signal transduction | | GO:0010667 | ; P:negative regulation of cardiac muscle cell apoptotic process | | GO:0006469 | ; P:negative regulation of protein kinase activity | | GO:0034122 | ; P:negative regulation of toll-like receptor signaling pathway | | GO:0030512 | ; P:negative regulation of transforming growth factor beta receptor signaling pathway | | GO:0018107 | ; P:peptidyl-threonine phosphorylation | | GO:0090004 | ; P:positive regulation of establishment of protein localization to plasma membrane | | GO:0010594 | ; P:regulation of endothelial cell migration | | GO:0043122 | ; P:regulation of I-kappaB kinase/NF-kappaB cascade | | GO:0043304 | ; P:regulation of mast cell degranulation | | GO:0006355 | ; P:regulation of transcription, DNA-dependent | | GO:0007165 | ; P:signal transduction | | GO:0006351 | ; P:transcription, DNA-dependent | | GO:0003323 | ; P:type B pancreatic cell development |
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| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
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| Prints | |
| Created Date | 20-Feb-2013 |