EKS-MUM-00474

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00474

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/DMPK	470.4	4.0E-141	76	338	263

Status

Reviewed

Ensembl Protein

ENSMUSP00000069549

UniProt Accession

P70335; Q8C3G4; Q8C7H0;

Protein Name

Rho-associated protein kinase 1

Protein Synonyms/Alias

Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK;

Gene Name

Rock1

Gene Synonyms/Alias

Rock1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000024290	ENSMUSP00000069549	ENSMUST00000067947

Organism

Mus musculus

Functional Description

Protein kinase which is a key regulator of actincytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Promotes keratinocyte terminal differentiation (By similarity). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.

Protein Length

1354

Protein Sequence
(FASTA)

MSTGDSFETR FEKIDNLLRD PKSEVNSDCL LDGLDALVYD LDFPALRKNK NIDNFLSRYK 60

DTINKIRDLR MKAEDYEVVK VIGRGAFGEV QLVRHKSTRK VYAMKLLSKF EMIKRSDSAF 120

FWEERDIMAF ANSPWVVQLF YAFQDDRYLY MVMEYMPGGD LVNLMSNYDV PEKWARFYTA 180

EVVLALDAIH SMGFIHRDVK PDNMLLDKSG HLKLADFGTC MKMNKEGMVR CDTAVGTPDY 240

ISPEVLKSQG GDGYYGRECD WWSVGVFLYE MLVGDTPFYA DSLVGTYSKI MNHKNSLTFP 300

DDNDISKEAK NLICAFLTDR EVRLGRNGVE EIKRHLFFKN DQWAWETLRD TVAPVVPDLS 360

SDIDTSNFDD LEEDKGDEET FPIPKAFVGN QLPFVGFTYY SNRRYLPSAN ASENRSSSNV 420

DKSLQESLQK TIYKLEEQLH NEMQLKDEME QKCRTSNLKL DKIMKELDEE GNQRRNLESA 480

VSQIEKEKML LQHRINEYQR KVEQENEKRR NIENEVSTLK DQLEDLRKAS QTSQLANEKL 540

TQLQKQLEEA NDLLRTESDT AVRLRKSHTE MSKSISQLES LNRELQERNR ILENSKSQAD 600

KDYYQLQAVL EAERRDRGHD SEMIGDLQAR ITSLQEEVKH LKHNLERVEG ERKEAQDMLN 660

HSEKEKNNLE IDLNYKLKSI QQRLEQEVNE HKVTKARLTD KHQSIEEAKS VAMCEMEKKL 720

KEEREAREKA ENRVVETEKQ CSMLDVDLKQ SQQKLEHLTE NKERMEDEVK NLALQLEQES 780

NKRLLLQNEL KTQAFEADNL KGLEKQMKQE INTLLEAKRL LEFELAQLTK QYRGNEGQMR 840

ELQDQLEAEQ YFSTLYKTQV KELKEEIEEK NRENLRKIQE LQSEKETLST QLDLAETKAE 900

SEQLARGILE EQYFELTQES KKAASRNRQE ITDKDHTVSR LEETNSVLTK DIEMLRKENE 960

ELNERMRTAE EEYKLKKEEE INNLKAAFEK NISTERTLKT QAVNKLAEIM NRKDFKIDRK 1020

KANTQDLRKK EKENRKLQLE LNQEREKFNQ MVVKHQKELN DMQAQLVEEC THRNELQMQL 1080

ASKESDIEQL RAKLLDLSDS TSVASFPSAD ETDGNLPESR IEGWLSVPNR GNIKRYGWKK 1140

QYVVVSSKKI LFYNDEQDKE QSSPSMVLDI DKLFHVRPVT QGDVYRAETE EIPKIFQILY 1200

ANEGECRKDI EVEPVQQGEK TNFQNHKGHE FIPTLYHFPA NCEACAKPLW HVFKPPPALE 1260

CRRCHVKCHR DHLDKKEDLI SPCKVSYDVT SARDMLLLAC SQDEQKKWVT HLVKKIPKNP 1320

PSGFVRASPR TLSTRSTANQ SFRKVVKNTS GKTS 1354

Nucleotide Sequence
(FASTA)

ATGTCGACTG GGGACAGTTT TGAGACTCGG TTTGAAAAAA TCGACAACCT GCTGCGGGAT 60

CCCAAATCGG AAGTGAATTC AGATTGTTTG CTGGATGGAT TGGATGCCCT GGTTTATGAT 120

TTGGATTTTC CTGCCTTAAG AAAAAACAAA AATATTGACA ACTTTCTAAG CAGATATAAA 180

GACACAATAA ATAAAATTAG AGATTTACGG ATGAAAGCAG AAGATTATGA AGTGGTAAAG 240

GTAATCGGCA GAGGTGCATT TGGAGAAGTT CAATTGGTGA GGCATAAATC CACTAGGAAG 300

GTTTATGCTA TGAAGCTGCT CAGCAAATTT GAAATGATTA AGAGATCGGA TTCTGCTTTT 360

TTCTGGGAAG AAAGGGACAT CATGGCGTTT GCCAATAGTC CTTGGGTTGT TCAGCTTTTT 420

TATGCATTCC AAGATGACCG GTATCTCTAC ATGGTGATGG AGTACATGCC TGGCGGAGAC 480

CTTGTCAACC TGATGAGCAA CTATGACGTG CCTGAAAAAT GGGCAAGATT CTATACTGCA 540

GAAGTAGTTC TTGCATTGGA TGCAATCCAT TCCATGGGTT TTATTCATAG AGATGTAAAG 600

CCTGATAACA TGCTGCTGGA TAAGTCTGGA CATTTGAAGT TAGCAGATTT TGGTACTTGT 660

ATGAAGATGA ATAAGGAAGG TATGGTACGA TGTGACACAG CAGTTGGAAC ACCTGATTAT 720

ATCTCACCCG AAGTATTAAA ATCCCAAGGC GGTGATGGCT ATTATGGACG AGAGTGTGAC 780

TGGTGGTCAG TTGGGGTATT TCTATATGAA ATGCTCGTAG GTGACACACC TTTCTATGCA 840

GATTCTTTGG TTGGGACTTA CAGTAAAATT ATGAACCATA AAAATTCACT TACTTTCCCT 900

GATGATAATG ACATATCAAA AGAGGCAAAA AATCTCATTT GTGCCTTCCT TACAGACAGA 960

GAAGTGAGAT TGGGCAGAAA TGGTGTAGAG GAAATCAAAC GTCATCTCTT CTTCAAAAAT 1020

GATCAGTGGG CTTGGGAAAC GCTCCGAGAC ACTGTAGCAC CAGTTGTGCC TGATTTAAGT 1080

AGTGACATTG ATACTAGTAA TTTTGATGAC TTAGAAGAAG ATAAAGGAGA TGAAGAAACA 1140

TTTCCTATTC CAAAAGCTTT TGTTGGCAAT CAGCTACCTT TTGTAGGATT TACATATTAT 1200

AGCAATCGTA GATACTTACC TTCAGCAAAT GCCAGTGAGA ACAGATCTAG CTCCAATGTG 1260

GATAAAAGCT TGCAGGAAAG TTTACAGAAA ACAATCTACA AGCTTGAAGA GCAACTGCAT 1320

AATGAAATGC AATTAAAAGA TGAAATGGAG CAGAAATGCA GAACTTCAAA CTTAAAACTA 1380

GACAAGATAA TGAAAGAATT GGATGAAGAG GGGAATCAAA GAAGAAATCT AGAATCTGCA 1440

GTGTCTCAAA TTGAGAAAGA AAAGATGTTG CTACAACATA GAATTAATGA GTACCAAAGA 1500

AAAGTGGAAC AGGAAAATGA GAAGAGAAGA AATATAGAAA ATGAAGTTTC TACACTAAAG 1560

GACCAGTTAG AAGACTTACG GAAAGCAAGT CAGACCTCAC AGCTTGCTAA TGAGAAGCTG 1620

ACACAGTTAC AGAAGCAGTT AGAAGAAGCG AATGACTTAC TTAGGACAGA ATCAGACACA 1680

GCTGTAAGAT TAAGGAAGAG TCACACAGAG ATGAGCAAGT CAATTAGTCA GCTAGAGTCC 1740

CTGAACAGAG AATTACAGGA AAGAAACAGA ATTTTAGAGA ATTCCAAGTC ACAAGCAGAC 1800

AAGGATTACT ACCAGCTGCA AGCTGTATTG GAAGCTGAGC GAAGAGACAG AGGGCATGAT 1860

TCTGAGATGA TTGGAGACCT TCAAGCACGA ATTACATCTT TACAAGAAGA AGTGAAACAT 1920

CTCAAACATA ATCTTGAAAG AGTGGAAGGA GAAAGAAAAG AGGCTCAAGA CATGCTTAAT 1980

CATTCAGAAA AGGAAAAGAA TAATTTGGAG ATAGATTTGA ATTACAAGCT CAAGTCTATA 2040

CAACAGCGGT TAGAACAAGA AGTAAATGAA CATAAAGTCA CCAAAGCACG CCTAACTGAC 2100

AAGCACCAAT CTATCGAAGA GGCAAAGTCT GTTGCCATGT GTGAAATGGA AAAAAAGCTG 2160

AAGGAAGAGA GAGAAGCTCG AGAGAAGGCA GAAAATAGAG TTGTTGAGAC TGAGAAACAA 2220

TGTTCCATGC TAGATGTTGA CCTGAAACAG TCTCAGCAGA AGCTAGAACA TTTGACCGAA 2280

AATAAAGAAA GGATGGAGGA TGAAGTTAAG AATCTCGCCC TGCAGCTGGA ACAGGAATCA 2340

AATAAACGGC TCTTGTTACA GAATGAATTG AAGACTCAAG CATTTGAAGC AGACAATTTA 2400

AAAGGTTTAG AAAAACAGAT GAAACAGGAA ATAAATACTT TATTGGAAGC AAAGAGATTG 2460

TTAGAATTTG AGTTAGCTCA GCTTACAAAG CAATATAGGG GAAATGAAGG GCAAATGCGG 2520

GAGTTACAAG ATCAACTTGA AGCTGAGCAG TATTTCTCTA CACTTTATAA AACCCAAGTA 2580

AAGGAGCTTA AAGAAGAAAT TGAAGAAAAA AACCGAGAAA ATTTAAGGAA AATACAGGAA 2640

CTGCAAAGTG AAAAAGAAAC TCTCTCTACT CAGTTGGATT TAGCAGAAAC AAAAGCTGAG 2700

TCTGAGCAGC TGGCGCGAGG GATTCTGGAA GAACAGTATT TTGAGTTGAC TCAGGAAAGC 2760

AAGAAAGCTG CTTCAAGAAA TAGACAAGAG ATTACAGATA AAGATCATAC TGTTAGTCGG 2820

CTTGAAGAAA CAAACAGTGT GCTAACCAAA GATATTGAAA TGCTAAGAAA AGAAAATGAA 2880

GAGCTAAATG AAAGAATGAG GACAGCAGAA GAAGAATATA AATTGAAGAA GGAGGAAGAA 2940

ATAAATAATC TTAAGGCTGC TTTTGAAAAG AATATCAGTA CCGAACGGAC CCTTAAAACA 3000

CAGGCTGTTA ACAAGTTGGC AGAAATAATG AATCGCAAAG ATTTTAAAAT TGATCGAAAG 3060

AAAGCTAATA CACAAGATTT AAGAAAAAAA GAAAAGGAAA ATAGAAAGCT ACAACTGGAA 3120

CTTAACCAAG AGAGAGAAAA ATTTAACCAA ATGGTGGTGA AACACCAGAA GGAGCTGAAT 3180

GACATGCAAG CGCAATTGGT AGAAGAATGT ACACATAGAA ATGAACTTCA AATGCAGTTG 3240

GCTAGCAAAG AGAGTGATAT TGAGCAGTTG CGGGCAAAAC TTTTGGACCT TTCGGATTCA 3300

ACTAGTGTTG CTAGTTTTCC TAGTGCAGAT GAAACTGATG GAAACCTTCC AGAGTCAAGA 3360

ATTGAAGGTT GGCTTTCTGT ACCAAATAGA GGAAATATCA AAAGATATGG CTGGAAGAAA 3420

CAGTATGTTG TGGTAAGCAG CAAAAAGATC TTGTTCTATA ATGATGAACA AGATAAGGAA 3480

CAATCCAGTC CATCTATGGT GTTAGACATA GATAAATTGT TTCATGTCCG ACCTGTAACC 3540

CAAGGAGATG TGTACAGAGC AGAAACTGAA GAAATTCCTA AAATATTCCA GATACTATAT 3600

GCAAATGAAG GTGAATGTAG AAAAGATATA GAAGTGGAAC CGGTTCAACA AGGTGAAAAA 3660

ACTAATTTCC AGAATCACAA AGGCCATGAA TTCATTCCTA CCCTCTACCA CTTTCCTGCC 3720

AACTGTGAGG CCTGTGCCAA ACCTTTGTGG CATGTCTTTA AGCCACCACC TGCCCTAGAG 3780

TGTCGAAGAT GCCATGTTAA GTGCCACAGA GACCATCTAG ACAAGAAAGA GGACTTGATT 3840

TCCCCGTGCA AAGTAAGTTA CGATGTAACA TCAGCAAGAG ATATGCTGCT GTTAGCATGT 3900

TCTCAGGATG AACAAAAAAA GTGGGTTACT CATCTAGTAA AGAAGATTCC TAAGAACCCA 3960

CCATCTGGCT TTGTCCGTGC CTCTCCTCGA ACGCTGTCAA CAAGATCCAC TGCAAATCAG 4020

TCTTTCCGCA AAGTAGTCAA AAATACATCT GGAAAAACTA GTTAA 4065

Domain Profile

S: 1     feilkviGrGafgevavvrekatekvyamkilkklellkraesaffreerdvlvkadsew  60

         +e++kviGrGafgev++vr+k+t+kvyamk+l+k+e++kr++saff+eerd++++a+s+w

Q: 76    YEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPW  135

         89**********************************************************

S: 61    ivqlqyafqdekylylvmeylvGGdlltllskyedklpeelarfylaelvlaldsvhelg  120

         +vql+yafqd++yly+vmey++GGdl++l+s+y+  +pe++arfy+ae+vlald++h++g

Q: 136   VVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYD--VPEKWARFYTAEVVLALDAIHSMG  193

         **********************************..9***********************

S: 121   yvhrdikpdnilldksGhikladfGsclklnedgtvkssvavGtpdyispevlkaleegk  180

         ++hrd+kpdn+lldksGh+kladfG+c+k+n++g+v++++avGtpdyispevlk+++ g+

Q: 194   FIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQG-GD  252

         ********************************************************9.**

S: 181   geygkecdwwslGvilyellvgetpfyaeslvetyskilnhkeslkfpedekvseeakdl  240

         g+yg+ecdwws+Gv+lye+lvg+tpfya+slv tyski+nhk+sl+fp+d+++s+eak+l

Q: 253   GYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNL  312

         ************************************************************

S: 241   ikklltsreerlgregiedlkkhpff  266

         i+++lt+re rlgr+g+e++k+h ff

Q: 313   ICAFLTDREVRLGRNGVEEIKRHLFF  338

         *************************9

Domain Sequence
(FASTA)

YEVVKVIGRG AFGEVQLVRH KSTRKVYAMK LLSKFEMIKR SDSAFFWEER DIMAFANSPW 60

VVQLFYAFQD DRYLYMVMEY MPGGDLVNLM SNYDVPEKWA RFYTAEVVLA LDAIHSMGFI 120

HRDVKPDNML LDKSGHLKLA DFGTCMKMNK EGMVRCDTAV GTPDYISPEV LKSQGGDGYY 180

GRECDWWSVG VFLYEMLVGD TPFYADSLVG TYSKIMNHKN SLTFPDDNDI SKEAKNLICA 240

FLTDREVRLG RNGVEEIKRH LFF 263

Keyword

Acetylation; Alternative splicing; Apoptosis; ATP-binding; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Golgi apparatus; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0032059	; C:bleb
GO:0005814	; C:centriole
GO:0000139	; C:Golgi membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0030036	; P:actin cytoskeleton organization
GO:0003383	; P:apical constriction
GO:0006915	; P:apoptotic process
GO:0032060	; P:bleb assembly
GO:0000910	; P:cytokinesis
GO:0050901	; P:leukocyte tethering or rolling
GO:0022614	; P:membrane to membrane docking
GO:0016525	; P:negative regulation of angiogenesis
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:0032970	; P:regulation of actin filament-based process
GO:0045616	; P:regulation of keratinocyte differentiation
GO:0007266	; P:Rho protein signal transduction

KEGG

mmu:19877

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011072	; HR1_rho-bd.
IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR017892	; Pkinase_C.
IPR001849	; Pleckstrin_homology.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR020684	; Rho-assoc_coiled-coil_kin.
IPR015008	; Rho-bd.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF02185	; HR1; 1.
PF00169	; PH; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.
PF08912	; Rho_Binding; 1.

SMARTs

SM00109	; C1; 1.
SM00233	; PH; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; FALSE_NEG.
PS50081	; ZF_DAG_PE_2; 1.

Prints

Created Date

20-Feb-2013