EKS-MUM-00169

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00169

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/RSKb	425.1	2.3E-127	415	674	260

Status

Reviewed

Ensembl Protein

ENSMUSP00000025903

UniProt Accession

Q9Z2B9; Q3U3M8; Q91X18;

Protein Name

Ribosomal protein S6 kinase alpha-4

Protein Synonyms/Alias

S6K-alpha-4; 90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; RSK-like protein kinase; RLSK;

Gene Name

Rps6ka4

Gene Synonyms/Alias

Rps6ka4; Msk2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000024952	ENSMUSP00000025903	ENSMUST00000025903
ENSMUSG00000024952	ENSMUSP00000131581	ENSMUST00000170516

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase that is required for themitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide- stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity).

Protein Length

773

Protein Sequence
(FASTA)

MGDEDEDEGC AVELQITEAN LTGHEEKVSV ENFALLKVLG TGAYGKVFLV RKTGGHDAGK 60

LYAMKVLRKA ALVQRAKTQE HTRTERSVLE LVRQAPFLVT LHYAFQTDAK LHLILDYVSG 120

GEMFTHLYQR QYFKEAEVRV YGGEIVLALE HLHKLGIIYR DLKLENVLLD SEGHIVLTDF 180

GLSKEFLTEE KERTFSFCGT IEYMAPEIIR SKAGHGKAVD WWSLGILLFE LLTGASPFTL 240

EGERNTQAEV SRRILKCSPP FPLRIGPVAQ DLLQRLLCKD PKKRLGAGPQ GAQEVKSHPF 300

FQGLDWVALA ARKIPAPFRP QIRSELDVGN FAEEFTRLEP VYSPAGSPPP GDPRIFQGYS 360

FVAPSILFDH NNAVMADVLQ APGAGYRPGR AAVARSAMMQ DSPFFQQYEL DLREPALGQG 420

SFSVCRRCRQ RQSGQEFAVK ILSRRLEENT QREVAALRLC QSHPNVVNLH EVLHDQLHTY 480

LVLELLRGGE LLEHIRKKRL FSESEASQIL RSLVSAVSFM HEEAGVVHRD LKPENILYAD 540

DTPGAPVKII DFGFARLRPQ SPAEPMQTPC FTLQYAAPEL LAQQGYDESC DLWSLGVILY 600

MMLSGQVPFQ GASGQGGQSQ AAEIMCKIRE GRFSLDGEAW QGVSEEAKEL VRGLLTVDPA 660

KRLKLEGLRS SSWLQDGSAR SSPPLRTPDV LESSGPAVRS GLNATFMAFN RGKREGFFLK 720

SVENAPLAKR RKQKLRSAAA SRRGSPVPAS SGRLPASAAK GTTRRANGPL SPS 773

Nucleotide Sequence
(FASTA)

ATGGGAGACG AGGATGAGGA CGAGGGCTGC GCCGTGGAGC TGCAGATCAC CGAAGCCAAC 60

CTCACCGGGC ATGAGGAGAA GGTGAGCGTG GAGAACTTCG CGCTGCTCAA GGTGCTGGGC 120

ACGGGAGCCT ATGGGAAGGT GTTCCTGGTG CGGAAGACGG GTGGGCACGA CGCGGGCAAG 180

CTCTATGCCA TGAAGGTGCT ACGCAAGGCG GCGTTGGTGC AGCGCGCGAA GACACAGGAG 240

CATACCCGCA CCGAACGCTC GGTGCTGGAG CTGGTTCGCC AAGCACCCTT CCTGGTCACA 300

CTGCACTACG CCTTCCAGAC GGATGCCAAG CTGCACCTCA TCCTGGACTA CGTGAGCGGT 360

GGTGAGATGT TCACTCACCT CTACCAGCGC CAGTACTTCA AGGAGGCTGA GGTTCGAGTG 420

TATGGGGGCG AGATTGTGCT GGCCCTGGAA CACCTGCACA AGCTGGGTAT CATCTACCGG 480

GACCTGAAGC TGGAGAACGT CTTACTTGAC TCAGAAGGTC ACATCGTCCT TACAGACTTT 540

GGGCTGAGCA AGGAGTTCCT GACGGAGGAG AAAGAGCGGA CCTTCTCCTT CTGTGGCACA 600

ATCGAGTACA TGGCTCCCGA AATCATCCGA AGCAAGGCTG GACATGGCAA GGCTGTGGAC 660

TGGTGGAGCC TGGGTATCCT GCTCTTCGAG CTGCTGACAG GGGCCTCACC CTTCACACTG 720

GAGGGAGAGA GGAACACTCA GGCTGAGGTG TCCCGACGGA TCTTGAAGTG CTCCCCTCCC 780

TTCCCTCTCC GGATTGGGCC TGTGGCACAG GACCTGCTAC AGCGGCTGCT GTGCAAGGAC 840

CCTAAGAAGA GGTTGGGCGC AGGTCCCCAG GGTGCGCAGG AAGTCAAGAG TCACCCCTTC 900

TTCCAGGGTC TGGACTGGGT GGCTCTGGCT GCCAGAAAGA TCCCAGCCCC ATTCCGGCCC 960

CAGATCCGCT CAGAGCTGGA TGTGGGGAAT TTTGCGGAGG AATTCACCCG GCTGGAGCCC 1020

GTCTACTCCC CTGCAGGCAG CCCTCCACCT GGGGACCCTC GGATCTTTCA GGGATACTCC 1080

TTCGTGGCTC CGTCCATCCT CTTTGACCAC AACAATGCAG TGATGGCTGA TGTACTGCAG 1140

GCACCGGGTG CCGGATACAG GCCCGGCAGG GCAGCAGTTG CCAGGAGTGC CATGATGCAG 1200

GACTCGCCTT TCTTCCAGCA GTACGAACTG GACCTTCGGG AGCCAGCGCT GGGGCAGGGC 1260

AGCTTCTCTG TGTGTCGGAG ATGTAGGCAG CGCCAGAGCG GCCAGGAGTT TGCTGTCAAG 1320

ATCCTCAGCC GCAGGCTGGA GGAGAACACT CAGCGAGAGG TGGCTGCTCT TCGCCTGTGC 1380

CAGTCACACC CCAACGTGGT GAATCTGCAT GAGGTGCTTC ATGACCAGCT ACACACTTAC 1440

CTGGTCCTGG AGTTGCTGCG AGGCGGAGAG CTATTGGAAC ACATCCGCAA GAAGCGGCTC 1500

TTCAGCGAGT CGGAGGCCAG CCAGATCCTT CGGAGCCTGG TTTCGGCCGT GAGCTTCATG 1560

CACGAGGAGG CAGGCGTGGT GCACCGCGAC CTGAAACCCG AGAACATCTT GTACGCGGAC 1620

GACACTCCCG GGGCCCCGGT GAAGATCATC GACTTCGGGT TCGCGCGACT GCGGCCCCAG 1680

AGCCCGGCAG AGCCCATGCA GACTCCTTGC TTCACACTGC AGTACGCTGC ACCCGAGCTG 1740

CTGGCACAGC AGGGCTACGA TGAGTCCTGC GATCTATGGA GCCTGGGTGT CATTCTGTAC 1800

ATGATGCTGT CTGGCCAGGT TCCCTTCCAA GGGGCCTCCG GCCAGGGTGG ACAGAGTCAG 1860

GCAGCTGAGA TCATGTGCAA GATCCGTGAA GGGCGCTTCT CCCTGGACGG GGAAGCCTGG 1920

CAAGGTGTGT CGGAGGAAGC CAAGGAGCTG GTCCGAGGGC TACTGACAGT CGACCCCGCC 1980

AAGCGGCTGA AGCTGGAGGG GCTGCGTAGC AGCTCGTGGC TTCAGGACGG CAGCGCGCGC 2040

TCCTCGCCCC CGCTCCGCAC GCCGGATGTG CTGGAGTCCT CTGGGCCAGC TGTGCGTTCC 2100

GGGCTCAATG CCACTTTCAT GGCGTTCAAC CGAGGCAAGC GCGAGGGCTT CTTTCTCAAG 2160

AGTGTAGAGA ATGCGCCTCT GGCCAAGAGG CGCAAGCAGA AGCTCCGGAG CGCGGCCGCC 2220

TCCCGCCGAG GCTCCCCAGT GCCTGCCTCC TCCGGTCGCC TACCAGCCTC TGCCGCTAAG 2280

GGGACAACTC GCCGAGCCAA CGGCCCCTTG TCCCCTTCCT AG 2322

Domain Profile

S: 5     edlGvGsysvckrcvhkatnqefavkiidks.krdtseeieillryeqhpnivtlkdvyd  63

           lG+Gs+svc+rc++++++qefavki++++ + +t++e+++l+++++hpn+v+l++v +

Q: 415   PALGQGSFSVCRRCRQRQSGQEFAVKILSRRlEENTQREVAALRLCQSHPNVVNLHEVLH  474

         579*********************************************************

S: 64    dgkyvylvlellkGGelldrikkkkffsereasailrtlvkaveylheq.Gvvhrdlkpe  122

         d++++ylvlell+GGell++i+kk+ fse+eas+ilr+lv+av+++he+ Gvvhrdlkpe

Q: 475   DQLHTYLVLELLRGGELLEHIRKKRLFSESEASQILRSLVSAVSFMHEEaGVVHRDLKPE  534

         ***********************************************999**********

S: 123   nilyvdesdnaesikiidfGfaklkraeng.llktpcytlnfvapevlkrqGydeacdlw  181

         nily+d++++a+ +kiidfGfa+l+++++  +++tpc+tl+++ape+l++qGyde+cdlw

Q: 535   NILYADDTPGAP-VKIIDFGFARLRPQSPAePMQTPCFTLQYAAPELLAQQGYDESCDLW  593

         ************.**************9977*****************************

S: 182   slGvllytmlaGyvpf....angpedtaeeilakikeGkfslsGeawdsvseeakdlvsk  237

         slGv+ly+ml+G+vpf    ++g++++a+ei++ki+eG+fsl+Geaw++vseeak+lv++

Q: 594   SLGVILYMMLSGQVPFqgasGQGGQSQAAEIMCKIREGRFSLDGEAWQGVSEEAKELVRG  653

         ****************999889**************************************

S: 238   lltvdpakrlkleqllkhswl  258

         lltvdpakrlkle+l+++swl

Q: 654   LLTVDPAKRLKLEGLRSSSWL  674

         ********************7

Domain Sequence
(FASTA)

PALGQGSFSV CRRCRQRQSG QEFAVKILSR RLEENTQREV AALRLCQSHP NVVNLHEVLH 60

DQLHTYLVLE LLRGGELLEH IRKKRLFSES EASQILRSLV SAVSFMHEEA GVVHRDLKPE 120

NILYADDTPG APVKIIDFGF ARLRPQSPAE PMQTPCFTLQ YAAPELLAQQ GYDESCDLWS 180

LGVILYMMLS GQVPFQGASG QGGQSQAAEI MCKIREGRFS LDGEAWQGVS EEAKELVRGL 240

LTVDPAKRLK LEGLRSSSWL 260

Keyword

ATP-binding; Complete proteome; Inflammatory response; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Stress response; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0048273	; F:mitogen-activated protein kinase p38 binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0043987	; P:histone H3-S10 phosphorylation
GO:0043988	; P:histone H3-S28 phosphorylation
GO:0006954	; P:inflammatory response
GO:0070498	; P:interleukin-1-mediated signaling pathway
GO:0007243	; P:intracellular protein kinase cascade
GO:0035066	; P:positive regulation of histone acetylation
GO:0033129	; P:positive regulation of histone phosphorylation
GO:0045944	; P:positive regulation of transcription from RNA polymerase II promoter
GO:0006355	; P:regulation of transcription, DNA-dependent

KEGG

mmu:56613

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR016239	; Ribosomal_S6_kinase_II.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 2.
PF00433	; Pkinase_C; 1.

SMARTs

SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 2.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 2.
PS50011	; PROTEIN_KINASE_DOM; 2.
PS00108	; PROTEIN_KINASE_ST; 2.

Prints

Created Date

20-Feb-2013