| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00294 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| Other/PLK | 390.9 | 6.8E-117 | 12 | 265 | 254 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000128916 |
| UniProt Accession | Q64702; Q3UVA3; Q6PEP6; Q78EG6; Q80UT6; Q8R0I5; Q9CVR6; Q9CVU6; |
| Protein Name | Serine/threonine-protein kinase PLK4 |
| Protein Synonyms/Alias | Polo-like kinase 4; PLK-4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase Sak; |
| Gene Name | Plk4 |
| Gene Synonyms/Alias | Plk4; Sak, Stk18; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Serine/threonine-protein kinase that plays a centralrole in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2 (By similarity). Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. |
| Protein Length | 464 |
Protein Sequence
(FASTA) | | MAACIGERIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE 60 | | VKIHCQLKHP SVLELYNYFE DNNYVYLVLE MCHNGEMNRY LKNRMKPFSE REARHFMHQI 120 | | ITGMLYLHSH GILHRDLTLS NILLTRNMNI KIADFGLATQ LNMPHEKHYT LCGTPNYISP 180 | | EIATRSAHGL ESDIWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PAFLSREAQD 240 | | LIHQLLRRNP ADRLSLSSVL DHPFMSRNPS PKSKDVGTVE DSMDSGHATL STTITASSGT 300 | | SLSGSLLDRR LLVGQPLPNK ITVFQKNKNS SDFSSGDGSN FCTQWGNPEQ EANSRGRGRV 360 | | IEDAEERPHS RYLRRAHSSD RASPSNQSRA KTYSVERCHS VEMLSKPRRS LDENQHRYSP 420 | | TKSNVNVLTS LNTKQPIVKD LLKDRIMTEQ YKDNLLNLLN KFDR 464 |
|
Nucleotide Sequence
(FASTA) | | ATGGCGGCGT GCATCGGGGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTCGGTAAA 60 | | GGATCATTTG CTGGTGTCTA CAGAGCTGAG TCCATACACA CTGGTTTGGA AGTTGCAATC 120 | | AAAATGATAG ATAAGAAAGC CATGTACAAA GCTGGAATGG TACAGAGAGT CCAAAATGAG 180 | | GTGAAAATAC ATTGCCAGTT GAAACACCCC TCTGTCTTGG AGCTCTATAA TTACTTTGAA 240 | | GATAACAATT ATGTCTACCT GGTATTGGAA ATGTGCCACA ATGGAGAAAT GAACAGATAT 300 | | CTGAAGAACA GAATGAAGCC TTTCTCAGAA AGGGAAGCTA GGCACTTCAT GCACCAGATT 360 | | ATCACAGGAA TGTTATATCT TCATTCTCAT GGCATATTGC ACCGGGACCT CACACTCTCT 420 | | AACATCTTAC TTACGCGGAA TATGAACATA AAAATTGCTG ACTTTGGACT AGCAACGCAG 480 | | TTGAATATGC CACATGAAAA GCACTATACA CTCTGTGGGA CTCCTAATTA TATTTCACCA 540 | | GAAATTGCAA CTCGAAGTGC ACATGGACTT GAATCTGATA TTTGGTCATT GGGCTGTATG 600 | | TTTTATACGT TACTTATTGG AAGACCACCT TTTGACACTG ACACAGTCAA GAACACATTG 660 | | AACAAAGTAG TCCTGGCAGA TTATGAAATG CCAGCCTTTT TGTCACGAGA GGCCCAGGAC 720 | | CTTATCCACC AGTTACTTCG TAGAAACCCT GCAGATCGGT TAAGTCTGTC TTCTGTGTTG 780 | | GACCATCCTT TCATGTCACG AAATCCTTCA CCAAAGAGTA AAGACGTAGG GACTGTAGAG 840 | | GACTCAATGG ATAGTGGGCA TGCTACACTT TCCACAACAA TTACAGCCTC TTCTGGTACC 900 | | AGTTTGAGTG GCAGCCTACT TGACAGAAGA CTTTTGGTTG GTCAACCACT TCCAAATAAA 960 | | ATTACTGTAT TTCAAAAAAA TAAAAATTCA AGTGACTTTT CTTCAGGAGA TGGAAGTAAT 1020 | | TTTTGTACTC AATGGGGAAA TCCAGAACAA GAAGCTAATA GTAGGGGACG GGGGAGAGTG 1080 | | ATTGAAGATG CAGAAGAGAG GCCGCATTCT CGATACCTGC GCAGAGCTCA TTCCTCTGAT 1140 | | AGAGCCAGCC CCTCTAATCA GTCTCGAGCA AAAACATACT CAGTAGAACG TTGTCACTCA 1200 | | GTAGAAATGC TTTCAAAGCC TAGAAGATCA CTGGATGAAA ATCAACACAG GTATTCACCC 1260 | | ACCAAAAGCA ATGTCAATGT TTTAACTTCA TTAAACACCA AACAGCCAAT AGTTAAGGAT 1320 | | CTTTTGAAAG ACCGTATAAT GACTGAGCAG TATAAGGATA ATCTTTTAAA CTTATTGAAC 1380 | | AAGTTTGATC GCTAA 1395 |
|
| Domain Profile | S: 1 yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn 60 | +++g+llGkG+fa +y++++++t+ ++a+k+++k++++k+ + ++v++e++ih++lkh++ | Q: 12 FKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPS 71 | 6899******************************************************** |
| S: 61 ivklyeffedkenvylvlelcsrrslaellkrr.kaltepevryylrqivsglkylhekr 119 | +++ly++fed+++vylvle+c++++++++lk+r k+++e+e+r++++qi++g++ylh++ | Q: 72 VLELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRmKPFSEREARHFMHQIITGMLYLHSHG 131 | ********************************99************************** |
| S: 120 ilhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevlskkghsle 179 | ilhrdl+l+n++l++++++ki+dfGlat+l++++e+++tlcGtPnyi+Pe++++++h+le | Q: 132 ILHRDLTLSNILLTRNMNIKIADFGLATQLNMPHEKHYTLCGTPNYISPEIATRSAHGLE 191 | ************************************************************ |
| S: 180 vdiWslGcilytllvGkPPfetkelketyekikkaeyslPsslskeaksliakllkkePe 239 | +diWslGc++ytll+G+PPf+t+++k+t++k++ a+y++P+ ls+ea++li++ll+++P+ | Q: 192 SDIWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPA 251 | ************************************************************ |
| S: 240 erpsleevlkdefl 253 | +r+sl++vl+++f+ | Q: 252 DRLSLSSVLDHPFM 265 | ************98 |
|
Domain Sequence
(FASTA) | | FKVGNLLGKG SFAGVYRAES IHTGLEVAIK MIDKKAMYKA GMVQRVQNEV KIHCQLKHPS 60 | | VLELYNYFED NNYVYLVLEM CHNGEMNRYL KNRMKPFSER EARHFMHQII TGMLYLHSHG 120 | | ILHRDLTLSN ILLTRNMNIK IADFGLATQL NMPHEKHYTL CGTPNYISPE IATRSAHGLE 180 | | SDIWSLGCMF YTLLIGRPPF DTDTVKNTLN KVVLADYEMP AFLSREAQDL IHQLLRRNPA 240 | | DRLSLSSVLD HPFM 254 |
|
| Keyword | 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
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| Prints | |
| Created Date | 20-Feb-2013 |