EPS-MUM-00026

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00026

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	193.6	1.2E-56	154	408	255

Status

Reviewed

Ensembl Protein

ENSMUSP00000027373

UniProt Accession

Q8CGA0;

Protein Name

Protein phosphatase 1F

Protein Synonyms/Alias

Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase;

Gene Name

Ppm1f

Gene Synonyms/Alias

Ppm1f;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000026181	ENSMUSP00000027373	ENSMUST00000027373

Organism

Mus musculus

Functional Description

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis (By similarity).

Protein Length

452

Protein Sequence
(FASTA)

MASGAAQNSS QMACDSEIPG FLDAFLQDFP APLSLESPLP WKVPGTVLSQ EEVEAELIEL 60

ALGFLGSRNA PPSFAVAVTH EAISQLLQTD LSEFKRLPEQ EEEEEEEEEE KALVTLLDAK 120

GLARSFFNCL WKVCSQWQKQ VPLTAQAPQW QWLVSIHAIR NTRRKMEDRH VSLPAFNHLF 180

GLSDSVHRAY FAVFDGHGGV DAARYASVHV HTNASHQPEL RTNPAAALKE AFRLTDEMFL 240

QKAKRERLQS GTTGVCALIA GAALHVAWLG DSQVILVQQG RVVKLMEPHK PERQDEKARI 300

EALGGFVSLM DCWRVNGTLA VSRAIGDVFQ KPYVSGEADA ASRELTGSED YLLLACDGFF 360

DVVPHHEVTG LVHGHLLRHK GNGMRIAEEL VAVARDRGSH DNITVMVVFL REPLELLEGG 420

VQGTGDAQAD VGSQDLSTGL SELEISNTSQ RS 452

Nucleotide Sequence
(FASTA)

ATGGCCTCTG GAGCCGCACA GAACAGCAGC CAGATGGCCT GTGACAGTGA GATCCCAGGC 60

TTCCTGGACG CATTCCTCCA AGACTTCCCA GCCCCACTGA GCCTGGAGAG CCCTTTGCCA 120

TGGAAGGTCC CAGGGACAGT CCTGAGCCAG GAAGAAGTGG AGGCTGAGCT GATTGAGCTG 180

GCATTGGGCT TCCTGGGCAG CAGGAACGCT CCCCCATCGT TTGCTGTGGC TGTGACCCAC 240

GAGGCAATTT CCCAGCTGCT GCAGACAGAC CTTTCTGAAT TTAAGAGGTT GCCTGAACAA 300

GAGGAGGAGG AGGAAGAGGA GGAAGAAGAG AAAGCCCTCG TGACCTTACT AGATGCCAAA 360

GGCCTGGCAC GGAGCTTCTT CAACTGCCTT TGGAAAGTAT GTAGCCAGTG GCAGAAGCAG 420

GTGCCGTTGA CTGCCCAGGC CCCTCAGTGG CAGTGGCTGG TCTCTATCCA TGCCATCCGG 480

AACACACGAC GCAAGATGGA GGACCGACAC GTGTCCCTTC CTGCTTTCAA CCATCTCTTC 540

GGCTTGTCTG ACTCTGTGCA CCGTGCCTAC TTTGCTGTGT TTGATGGTCA TGGAGGCGTG 600

GATGCAGCAA GGTATGCCTC TGTCCACGTG CATACCAATG CTTCTCACCA GCCAGAGCTG 660

CGCACAAACC CTGCAGCAGC TCTCAAAGAA GCCTTCCGGC TCACCGATGA GATGTTTCTC 720

CAGAAAGCCA AGCGAGAGCG ACTGCAGAGC GGTACCACGG GTGTGTGTGC ACTCATCGCG 780

GGAGCAGCCC TGCATGTTGC CTGGCTTGGA GACTCCCAGG TCATCCTGGT ACAGCAAGGA 840

CGGGTAGTGA AGCTGATGGA GCCGCACAAA CCGGAGCGAC AGGATGAGAA AGCACGAATT 900

GAAGCCCTGG GTGGCTTTGT TTCTCTCATG GACTGTTGGA GAGTCAACGG GACCCTGGCA 960

GTGTCTAGAG CCATTGGGGA TGTCTTCCAG AAGCCCTATG TGTCTGGGGA GGCCGATGCA 1020

GCATCCAGAG AGCTAACGGG CTCAGAGGAC TACCTGTTGC TTGCTTGTGA TGGTTTCTTC 1080

GATGTTGTCC CCCACCATGA AGTCACGGGT CTCGTCCATG GCCACTTGCT CAGGCACAAG 1140

GGCAATGGGA TGCGTATTGC TGAGGAGCTG GTGGCTGTGG CCCGTGACCG GGGCTCCCAT 1200

GACAACATTA CAGTCATGGT GGTCTTCCTT AGGGAGCCCC TGGAGCTGCT AGAGGGTGGA 1260

GTCCAGGGGA CAGGGGATGC CCAGGCAGAT GTAGGAAGCC AGGACTTGTC CACTGGCCTC 1320

TCAGAACTTG AGATCAGTAA TACCTCACAG AGAAGCTAA 1359

Domain Profile

S: 3     vgvvsvngrrnemedavtsspnflkspekkdr..laffgvyDGhggsevakfcseklhei  60

         v++ ++ + r++med++ s p f    + +d    a+f+v+DGhgg ++a+++s ++h+

Q: 154   VSIHAIRNTRRKMEDRHVSLPAFNHLFGLSDSvhRAYFAVFDGHGGVDAARYASVHVHTN  213

         6678999**************999987777775568************************

S: 61    laeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikdsklyva  120

            + e     +++  +al++aF  tde +  l++++ +  +sG+t v+ali +  l+va

Q: 214   ASHQPE----LRTNPAAALKEAFRLTDEMF--LQKAKRERLQSGTTGVCALIAGAALHVA  267

         ****77....6778889*************..9999999999******************

S: 121   nvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkdgaRvlGvLavsRalGd  180

          +GdS+++L ++g +v+l + Hkp+r+dE++Rie+ gg v l d+ Rv+G LavsRa+Gd

Q: 268   WLGDSQVILVQQGRVVKLMEPHKPERQDEKARIEALGGFVSLMDCWRVNGTLAVSRAIGD  327

         ********************************************99**************

S: 181   lylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle.kkeaeqeaa  239

         ++ kpyv+ e +  s +lt ++++l La+DG +dvv+  e+  lv+ +l  +k +   +a

Q: 328   VFQKPYVSGEADAASRELTGSEDYLLLACDGFFDVVPHHEVTGLVHGHLLrHKGNGMRIA  387

         *********************************************987764788999***

S: 240   kkLvklalargskdnvsaivv  260

         ++Lv++a++rgs+dn++++vv

Q: 388   EELVAVARDRGSHDNITVMVV  408

         *****************9997

Domain Sequence
(FASTA)

VSIHAIRNTR RKMEDRHVSL PAFNHLFGLS DSVHRAYFAV FDGHGGVDAA RYASVHVHTN 60

ASHQPELRTN PAAALKEAFR LTDEMFLQKA KRERLQSGTT GVCALIAGAA LHVAWLGDSQ 120

VILVQQGRVV KLMEPHKPER QDEKARIEAL GGFVSLMDCW RVNGTLAVSR AIGDVFQKPY 180

VSGEADAASR ELTGSEDYLL LACDGFFDVV PHHEVTGLVH GHLLRHKGNG MRIAEELVAV 240

ARDRGSHDNI TVMVV 255

Keyword

Apoptosis; Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0043234	; C:protein complex
GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0046872	; F:metal ion binding
GO:0035690	; P:cellular response to drug
GO:0097193	; P:intrinsic apoptotic signaling pathway
GO:0033137	; P:negative regulation of peptidyl-serine phosphorylation
GO:0044387	; P:negative regulation of protein kinase activity by regulation of protein phosphorylation
GO:0045892	; P:negative regulation of transcription, DNA-dependent
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0050921	; P:positive regulation of chemotaxis
GO:0043280	; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0010634	; P:positive regulation of epithelial cell migration
GO:0051894	; P:positive regulation of focal adhesion assembly
GO:0010628	; P:positive regulation of gene expression
GO:0045927	; P:positive regulation of growth
GO:0051496	; P:positive regulation of stress fiber assembly

KEGG

mmu:68606

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013