EPS-MUM-00021

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00021

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	239.3	1.4E-70	112	388	277

Status

Reviewed

Ensembl Protein

ENSMUSP00000027534

UniProt Accession

Q8R0F6; Q05CC3;

Protein Name

Integrin-linked kinase-associated serine/threonine phosphatase 2C

Protein Synonyms/Alias

ILKAP;

Gene Name

Ilkap

Gene Synonyms/Alias

Ilkap;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000026309	ENSMUSP00000027534	ENSMUST00000027534

Organism

Mus musculus

Functional Description

Protein phosphatase that may play a role in regulationof cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation (By similarity).

Protein Length

392

Protein Sequence
(FASTA)

MDLFGDLPEP ERAPRPSAGK EAQGRPVLFE DLPPASSTDS GSGGPLLFDD LPPAASGNSG 60

SLATSGSQVV KTEGKGAKRK APEEEKNGGE ELVEKKVCKA SSVIFGLKGY VAERKGEREE 120

MQDAHVILND ITQECNPPSS LITRVSYFAV FDGHGGIRAS KFAAQNLHQN LIRKFPKGDI 180

ISVEKTVKRC LLDTFKHTDE EFLKQASSQK PAWKDGSTAT CVLAVDNILY IANLGDSRAI 240

LCRYNEESQK HAALSLSKEH NPTQYEERMR IQKAGGNVRD GRVLGVLEVS RSIGDGQYKR 300

CGVTSVPDIR RCQLTPNDRF ILLACDGLFK VFTPEEAVNF ILSCLEDDKI QTREGKPAVD 360

ARYEAACNRL ANKAVQRGSA DNVTVMVVRI GH 392

Nucleotide Sequence
(FASTA)

ATGGACCTCT TCGGGGACCT GCCGGAGCCC GAGCGCGCGC CGCGGCCATC TGCCGGGAAG 60

GAAGCACAGG GACGACCCGT GCTCTTTGAG GACCTGCCCC CGGCCAGCAG TACTGACTCA 120

GGCTCTGGGG GACCTTTACT CTTTGATGAT CTTCCGCCTG CTGCCAGTGG CAATTCAGGT 180

TCTCTTGCCA CATCAGGTTC CCAGGTGGTG AAGACTGAAG GGAAAGGAGC AAAGAGGAAA 240

GCCCCTGAGG AGGAGAAGAA TGGCGGTGAA GAGCTTGTGG AAAAGAAAGT TTGTAAAGCC 300

TCTTCGGTGA TCTTTGGTCT GAAAGGCTAT GTGGCAGAGC GGAAGGGTGA GAGGGAGGAG 360

ATGCAGGATG CCCATGTCAT CCTGAACGAT ATCACTCAGG AGTGTAATCC TCCATCATCT 420

CTCATTACTC GGGTTTCATA CTTTGCTGTG TTTGATGGAC ATGGAGGAAT ACGAGCCTCG 480

AAATTTGCTG CACAGAATTT GCACCAGAAC TTAATCAGGA AATTTCCTAA AGGAGATATA 540

ATCAGTGTGG AGAAGACTGT AAAGAGGTGT CTGCTAGATA CTTTTAAGCA CACCGATGAA 600

GAGTTCCTGA AACAGGCTTC AAGCCAGAAG CCTGCCTGGA AAGACGGGTC CACTGCCACG 660

TGTGTCCTGG CTGTGGACAA CATCCTGTAT ATCGCCAACC TTGGAGATAG TCGGGCAATC 720

CTGTGTCGAT ATAATGAGGA AAGTCAGAAG CACGCAGCCT TAAGCCTCAG CAAAGAGCAC 780

AACCCAACTC AGTATGAAGA GCGCATGAGG ATACAGAAAG CTGGAGGGAA TGTCAGAGAT 840

GGGCGTGTCT TGGGCGTGCT GGAGGTATCG CGTTCCATTG GAGATGGGCA GTACAAGCGC 900

TGTGGGGTCA CATCTGTGCC TGATATCAGA CGCTGCCAGC TGACCCCCAA TGACAGGTTC 960

ATTTTGCTGG CTTGCGACGG GCTTTTCAAG GTCTTTACCC CAGAAGAAGC TGTGAACTTC 1020

ATCTTGTCCT GCCTTGAGGA TGACAAGATC CAGACCCGGG AAGGGAAGCC TGCTGTTGAT 1080

GCCCGCTATG AAGCTGCATG CAACAGGCTG GCCAACAAGG CAGTGCAGCG GGGCTCAGCA 1140

GACAACGTGA CGGTGATGGT GGTGAGGATA GGACACTGA 1179

Domain Profile

S: 6     vsvngrrnemedavtsspnflkspekkdr....laffgvyDGhggsevakfcseklheil  61

         ++ +g+r+em+da+ + +++ ++++  +     +++f+v+DGhgg +++kf++++lh+ l

Q: 112   AERKGEREEMQDAHVILNDITQECNPPSSlitrVSYFAVFDGHGGIRASKFAAQNLHQNL  171

         567899****************99988878999***************************

S: 62    aeele..sseseerkvkkalakaFlstdeelkvleeksldidk..sGstavvalikdskl  117

          +++   +  s e+ vk++l ++F++tdee+  l+++s+++ +   Gsta+++l  d+ l

Q: 172   IRKFPkgDIISVEKTVKRCLLDTFKHTDEEF--LKQASSQKPAwkDGSTATCVLAVDNIL  229

         ***9977778899******************..666666655577***************

S: 118   yvanvGdSravLcr.ngk.....avelssdHkpsredEleRieeaggsvilkdgaRvlGv  171

         y+an+GdSra+Lcr n++     a++ls++H+p++ +E+ Ri++agg+v +    RvlGv

Q: 230   YIANLGDSRAILCRyNEEsqkhaALSLSKEHNPTQYEERMRIQKAGGNVRDG---RVLGV  286

         **************7666799*****************************99...*****

S: 172   LavsRalGdlylk.pyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle  230

         L+vsR++Gd+++k   vt+ P++  ++lt++d+f+ La+DGl++v+++eeav+++ ++le

Q: 287   LEVSRSIGDGQYKrCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLE  346

         *************555********************************************

S: 231   ............kkeaeqeaakkLvklalargskdnvsaivv  260

                      +++ ++a+++L++ a++rgs dnv+++vv

Q: 347   ddkiqtregkpaVDARYEAACNRLANKAVQRGSADNVTVMVV  388

         **************************************9997

Domain Sequence
(FASTA)

AERKGEREEM QDAHVILNDI TQECNPPSSL ITRVSYFAVF DGHGGIRASK FAAQNLHQNL 60

IRKFPKGDII SVEKTVKRCL LDTFKHTDEE FLKQASSQKP AWKDGSTATC VLAVDNILYI 120

ANLGDSRAIL CRYNEESQKH AALSLSKEHN PTQYEERMRI QKAGGNVRDG RVLGVLEVSR 180

SIGDGQYKRC GVTSVPDIRR CQLTPNDRFI LLACDGLFKV FTPEEAVNFI LSCLEDDKIQ 240

TREGKPAVDA RYEAACNRLA NKAVQRGSAD NVTVMVV 277

Keyword

Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0045786	; P:negative regulation of cell cycle
GO:0006470	; P:protein dephosphorylation
GO:0007090	; P:regulation of S phase of mitotic cell cycle

KEGG

mmu:67444

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013