EKS-MUM-00471

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00471

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/DMPK	496.8	3.6E-149	77	343	267

Status

Reviewed

Ensembl Protein

ENSMUSP00000075980

UniProt Accession

Q3UU96; B2RY49; Q4V9U7; Q6ZQA9; Q8R495;

Protein Name

Serine/threonine-protein kinase MRCK alpha

Protein Synonyms/Alias

CDC42-binding protein kinase alpha;

Gene Name

Cdc42bpa

Gene Synonyms/Alias

Cdc42bpa; Kiaa0451;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000026490	ENSMUSP00000118039	ENSMUST00000145181
ENSMUSG00000026490	ENSMUSP00000106746	ENSMUST00000111117
ENSMUSG00000026490	ENSMUSP00000115261	ENSMUST00000143176
ENSMUSG00000026490	ENSMUSP00000116337	ENSMUST00000133890
ENSMUSG00000026490	ENSMUSP00000075980	ENSMUST00000076687
ENSMUSG00000026490	ENSMUSP00000095059	ENSMUST00000097450
ENSMUSG00000026490	ENSMUSP00000114333	ENSMUST00000135056
ENSMUSG00000026490	ENSMUSP00000095062	ENSMUST00000097453

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase which is an importantdownstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LRP35A, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake (By similarity).

Protein Length

1638

Protein Sequence
(FASTA)

MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 60

KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 120

CFREERDVLV NGDSKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEEMARF 180

YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 240

PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 300

FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 360

EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 420

GPTSLDLDVS VQRTLDNNLA TEAYERRIKR LEQEKLELTR KLQESTQTVQ ALQYSTVDGP 480

LTASKDLEIK SLKEEIEKLR KQVAEVNHLE QQLEEANSVR RELDDAFRQI KASEKQIKTL 540

QQEREELNKL EVHTEALIAE ASKDKKLREQ SEHYSKQLEN ELEGLKQKQI SYSPGICSIE 600

HQQEITKLKT DLEKKSIFYE EEISKREGIH ASEIKNLKKE LHDSEGQQLA LNKEILVLKD 660

KLEKTRRESQ SEREEFENEF KQQYEREKVL LTEENKKLTS ELDKLTSLYE SLSLRNQHLE 720

EEVKDLADKK ESVAHWEAQI TEIIQWVSDE KDARGYLQAL ASKMTEELEA LRNSSLGTRA 780

TDMPWKMRRF AKLDMSARLE LQSALDAEIR AKQAIQEELN KVKASNILTE CKLKDSEKKN 840

LELLSEIEQL IKDTEELRSE KGIEHQDSQH SFLAFLNTPT DALDQFEIAD CAPLPAHTPT 900

LRKKGCPAST GFPPKRKTHQ FFVKSFTAPT KCHQCTSLMV GLIRQGCSCE VCGFSCHITC 960

VNKAPTVCPV PPEQTKGPLG IDPQKGVGTA YEGHVRIPKP AGVKKGWQRA LAVVCDFKLF 1020

LYDIAEGKAS QPTSVISQVI DMRDEEFSVS SVLASDVIHA SRKDIPCIFR VTASQLSAPS 1080

NKCSILMLAD SENERSKWVG VLSELHKILK KNKFRDRSVY VPKEAYDSTL PLIKTTQAAA 1140

IIDHERIALG NEEGLFVVHV TKDEIVRVGD NKKIHQIELI PSDQLVAVIS GRNRHVRLFP 1200

MSALDGRETD FYKLAETKGC QTIAAGKVRH GALSCLCVAM KRQVLCYELF QSKTRHRKFK 1260

EIQVPCNVQW MAIFSEHLCV GFQSGFLRYP LNGEGGPCNM LHSNDHTLSF ISHQPMDALC 1320

AVEISNKEYL LCFNSIGIYT DCQGRRSRQQ ELMWPANPSS CCYNAPYLSV YSENAVDIFD 1380

VNSMEWIQTL PLKKVRPLNT EGSLNLLGLE TIRLIYFKNK MAEGDELVVP ETSDNSRKQM 1440

VRNINNKRRY SFRVPEEERM QQRREMLRDP EMRNKLISNP TNFNHIAHMG PGDGIQILKD 1500

LPMNPRPQES RTVFSGSVSI PSITKSRPEP GRSMSASSGL SARSSAQNGS ALKREFSGGS 1560

YNTKRQPMPS PSEGSLSSGG MDQGSDAPAR DYDGEDSDSP RHSTASNSSN LSSPPSPISP 1620

QKTKSLSLES TDRGSWDP 1638

Nucleotide Sequence
(FASTA)

ATGTCCGGAG AAGTGCGTTT GAGGCAGTTG GAGCAGTTTA TTTTAGATGG GCCAGCTCAG 60

ACCAATGGGC AGTGCTTCAG TGTGGAGACG CTACTGGACA TCCTCATCTG TCTTTACGAT 120

GAATGCAATA ATTCTCCATT GAGAAGAGAG AAGAACATTC TTGAATATCT AGAATGGGCT 180

AAACCATTTA CTTCTAAAGT GAAGCAAATG CGATTACATA GAGAAGACTT TGAAATATTA 240

AAGGTGATTG GTCGAGGAGC TTTTGGGGAG GTTGCTGTAG TTAAACTAAA AAATGCAGAT 300

AAAGTATTCG CCATGAAAAT TCTGAACAAG TGGGAAATGC TGAAAAGAGC TGAGACAGCG 360

TGCTTTCGTG AAGAGAGGGA TGTGTTGGTA AATGGAGACA GTAAGTGGAT AACCACTCTG 420

CACTATGCTT TCCAGGATGA CAATAACTTA TACCTGGTTA TGGATTATTA TGTTGGTGGG 480

GATTTACTTA CTCTGCTCAG CAAATTTGAA GATAGATTGC CTGAAGAGAT GGCTCGGTTT 540

TACTTGGCTG AGATGGTGAT AGCCATTGAC TCAGTTCACC AGCTCCACTA TGTTCACAGA 600

GACATCAAAC CTGACAATAT ATTGATGGAT ATGAATGGAC ATATTCGTTT AGCAGATTTT 660

GGTTCTTGTC TGAAGCTGAT GGAAGATGGA ACGGTCCAGT CCTCGGTGGC AGTTGGAACT 720

CCAGATTATA TTTCTCCGGA AATCCTTCAG GCTATGGAAG ACGGAAAAGG CAGATATGGA 780

CCAGAGTGTG ACTGGTGGTC CTTGGGGGTC TGTATGTATG AGATGCTTTA CGGAGAAACA 840

CCATTCTATG CAGAGTCTCT GGTGGAGACA TATGGAAAAA TTATGAATCA CAAAGAGCGG 900

TTTCAGTTTC CAGCCCAAGT GACAGACGTG TCCGAAAATG CTAAAGATCT TATTCGAAGA 960

CTCATTTGTA GTAGAGAACA TCGACTTGGC CAAAATGGAA TAGAAGACTT TAAGAAGCAT 1020

CCATTCTTCA GTGGAATTGA TTGGGATAAC ATTCGGAATT GTGAAGCACC TTACATCCCA 1080

GAAGTTAGTA GCCCGACAGA TACGTCAAAT TTTGATGTGG ATGACGACTG TTTAAAAAAT 1140

TCTGAAACAA TGCCCCCACC AACACATACT GCATTTTCTG GCCATCATCT ACCATTTGTT 1200

GGTTTTACAT ATACTAGTAG CTGTGTTCTT TCTGATCGGA GCTGTTTAAG AGTCACAGCA 1260

GGCCCCACCT CATTGGACCT TGATGTCAGT GTCCAGAGGA CTCTAGACAA TAACTTAGCA 1320

ACTGAGGCTT ATGAAAGGAG AATTAAGCGG CTTGAGCAGG AAAAGCTTGA ACTGACTAGA 1380

AAACTTCAAG AGTCAACACA GACTGTTCAA GCTCTGCAAT ATTCAACTGT TGATGGTCCA 1440

CTAACGGCAA GCAAAGATTT AGAAATTAAA AGTCTGAAAG AAGAGATTGA AAAACTAAGG 1500

AAACAAGTAG CAGAAGTGAA TCATTTGGAA CAACAACTTG AAGAAGCTAA TTCTGTGAGA 1560

CGAGAACTAG ATGATGCTTT CAGGCAAATC AAAGCTTCTG AAAAACAAAT CAAAACTTTG 1620

CAGCAGGAAA GAGAAGAACT AAATAAGCTG GAAGTTCACA CCGAAGCTCT AATTGCTGAA 1680

GCATCAAAGG ACAAGAAGCT CCGAGAGCAG AGCGAACATT ATTCTAAGCA ACTGGAAAAC 1740

GAGTTGGAGG GCCTGAAGCA AAAACAAATT AGTTACTCAC CAGGAATATG CAGCATAGAA 1800

CATCAGCAAG AGATAACTAA ACTGAAAACT GACTTGGAAA AGAAAAGTAT CTTTTATGAA 1860

GAAGAAATTT CTAAAAGAGA AGGAATTCAC GCAAGTGAGA TTAAAAACCT GAAGAAGGAG 1920

CTGCATGACT CAGAAGGCCA GCAGCTTGCT CTCAACAAAG AGATTCTGGT TTTAAAAGAC 1980

AAATTGGAAA AAACCAGGAG AGAAAGTCAA AGTGAAAGGG AAGAATTTGA AAATGAGTTC 2040

AAACAACAAT ATGAACGGGA AAAAGTATTA TTAACTGAAG AGAATAAAAA GTTAACGAGT 2100

GAACTCGATA AGCTGACTTC ACTGTATGAG AGCTTAAGTT TGCGCAACCA GCACTTAGAA 2160

GAAGAAGTCA AAGATCTAGC AGATAAGAAG GAATCAGTTG CCCACTGGGA AGCCCAAATC 2220

ACAGAGATAA TTCAGTGGGT GAGCGATGAG AAGGATGCAC GAGGCTATCT TCAGGCCTTA 2280

GCTTCTAAAA TGACTGAAGA GTTGGAAGCA TTAAGAAATT CCAGCTTGGG GACACGAGCA 2340

ACAGATATGC CCTGGAAAAT GCGTCGTTTT GCAAAACTGG ACATGTCGGC TAGACTAGAG 2400

TTGCAGTCTG CTCTGGATGC AGAAATAAGA GCTAAGCAGG CCATCCAGGA AGAGCTGAAT 2460

AAAGTCAAAG CATCTAACAT CTTAACAGAA TGTAAACTGA AAGATTCAGA GAAGAAGAAC 2520

TTGGAACTGT TGTCCGAAAT CGAGCAGCTG ATAAAGGACA CTGAAGAGCT TAGATCTGAA 2580

AAGGGTATAG AGCACCAAGA CTCACAGCAT TCTTTCTTGG CATTTTTGAA TACGCCTACC 2640

GATGCTCTGG ACCAATTTGA AATTGCAGAC TGTGCTCCAC TTCCAGCTCA CACACCAACC 2700

TTAAGGAAAA AGGGATGCCC GGCTTCAACT GGCTTTCCAC CTAAGCGCAA GACTCATCAA 2760

TTTTTCGTGA AGTCTTTCAC TGCCCCTACC AAGTGTCACC AGTGCACCTC CTTGATGGTC 2820

GGTTTGATAA GACAGGGCTG TTCCTGTGAA GTCTGTGGGT TCTCATGTCA TATAACTTGT 2880

GTGAACAAAG CCCCGACGGT TTGCCCAGTT CCTCCTGAGC AAACTAAAGG TCCACTGGGC 2940

ATAGACCCCC AGAAGGGAGT TGGGACGGCG TATGAGGGTC ATGTCAGGAT CCCTAAGCCG 3000

GCGGGAGTGA AGAAAGGGTG GCAGAGAGCA CTGGCTGTGG TCTGCGACTT CAAACTGTTT 3060

CTTTATGATA TCGCAGAGGG AAAAGCATCG CAGCCCACCT CCGTCATTAG TCAAGTGATT 3120

GACATGAGAG ATGAAGAATT TTCTGTGAGT TCAGTCTTAG CTTCTGATGT TATTCACGCA 3180

AGTCGAAAAG ATATCCCCTG TATATTCAGG GTCACAGCTT CCCAGCTCTC AGCACCTAGT 3240

AACAAATGTT CCATCCTGAT GCTAGCAGAC AGCGAGAATG AGAGGAGTAA GTGGGTGGGA 3300

GTGCTGAGTG AACTCCACAA GATTCTGAAG AAAAACAAGT TCAGAGACCG CTCAGTGTAT 3360

GTCCCCAAGG AGGCGTACGA CAGCACGCTA CCCCTCATTA AGACAACGCA GGCCGCTGCA 3420

ATCATAGATC ATGAACGGAT AGCTTTGGGA AATGAAGAAG GATTATTTGT TGTGCATGTC 3480

ACCAAAGATG AGATTGTTAG AGTTGGTGAT AATAAGAAAA TCCATCAGAT CGAACTCATT 3540

CCAAGTGACC AGCTTGTCGC TGTGATCTCA GGCCGAAATC GCCACGTCCG ACTTTTTCCT 3600

ATGTCAGCCT TGGATGGGCG AGAGACGGAT TTTTATAAAC TGGCAGAGAC TAAAGGGTGT 3660

CAGACCATAG CTGCTGGGAA AGTGCGCCAC GGAGCTCTGT CCTGCCTGTG TGTGGCCATG 3720

AAGAGACAGG TCCTCTGCTA TGAACTGTTC CAAAGCAAGA CCAGGCACAG GAAGTTTAAG 3780

GAGATTCAAG TCCCATGTAA TGTCCAGTGG ATGGCCATCT TCAGTGAGCA TCTCTGTGTG 3840

GGATTCCAGT CAGGATTTCT CAGATACCCC CTGAATGGAG AAGGAGGTCC ATGCAATATG 3900

CTCCATTCAA ATGACCATAC ACTGTCATTC ATTTCACACC AACCAATGGA CGCTCTCTGT 3960

GCAGTTGAGA TCTCCAACAA AGAGTACTTG CTGTGTTTTA ACAGCATTGG GATATACACA 4020

GACTGCCAGG GCCGAAGATC CAGACAGCAG GAACTGATGT GGCCAGCAAA TCCTTCCTCC 4080

TGTTGTTACA ATGCTCCATA TCTCTCCGTG TATAGTGAAA ACGCAGTTGA TATCTTTGAT 4140

GTAAACTCCA TGGAATGGAT ACAGACTCTT CCTCTCAAAA AGGTCCGACC TTTAAACACT 4200

GAAGGATCAT TAAATCTCTT AGGATTGGAG ACCATTAGAT TAATATATTT CAAAAATAAG 4260

ATGGCAGAAG GGGATGAACT TGTGGTTCCT GAAACATCAG ATAATAGTCG GAAACAAATG 4320

GTTAGAAATA TCAACAACAA ACGGCGTTAC TCCTTCCGAG TCCCTGAAGA AGAGAGGATG 4380

CAACAGAGGA GGGAGATGCT ACGAGATCCA GAAATGAGAA ATAAATTAAT TTCTAACCCA 4440

ACTAATTTTA ACCACATAGC ACACATGGGT CCTGGAGATG GAATACAGAT CCTAAAAGAC 4500

CTGCCCATGA ACCCACGGCC TCAGGAAAGC CGGACAGTAT TCAGTGGCTC CGTCAGTATT 4560

CCCTCCATCA CCAAGTCCCG CCCTGAGCCA GGCCGCTCCA TGAGTGCCAG CAGCGGCCTG 4620

TCTGCACGGT CGTCAGCCCA GAATGGCAGT GCACTAAAGA GGGAATTCTC TGGAGGAAGC 4680

TATAACACAA AGCGACAGCC CATGCCCTCT CCCTCGGAGG GCTCTTTGTC ATCAGGAGGC 4740

ATGGACCAAG GAAGTGACGC CCCTGCGAGG GACTATGATG GAGAGGACTC TGATTCTCCA 4800

AGGCATTCCA CAGCTTCCAA CAGCTCTAAC CTGAGCAGCC CCCCAAGTCC CATTTCACCC 4860

CAAAAGACCA AGAGCCTCTC CCTGGAGAGC ACAGACCGCG GGAGCTGGGA TCCATGA 4917

Domain Profile

S: 1     feilkviGrGafgevavvrekatekvyamkilkklellkraesaffreerdvlvkadsew  60

         feilkviGrGafgevavv+ k+++kv+amkil+k+e+lkrae+a+freerdvlv++ds+w

Q: 77    FEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKW  136

         89**********************************************************

S: 61    ivqlqyafqdekylylvmeylvGGdlltllskyedklpeelarfylaelvlaldsvhelg  120

         i++l+yafqd+++lylvm+y+vGGdlltllsk+ed+lpee+arfylae+v+a+dsvh+l+

Q: 137   ITTLHYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLH  196

         ************************************************************

S: 121   yvhrdikpdnilldksGhikladfGsclklnedgtvkssvavGtpdyispevlkaleegk  180

         yvhrdikpdnil+d++Ghi+ladfGsclkl+edgtv+ssvavGtpdyispe+l+a+e+gk

Q: 197   YVHRDIKPDNILMDMNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGK  256

         ************************************************************

S: 181   geygkecdwwslGvilyellvgetpfyaeslvetyskilnhkeslkfpede.kvseeakd  239

         g+yg+ecdwwslGv++ye+l+getpfyaeslvety+ki+nhke+++fp+++ +vse+akd

Q: 257   GRYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHKERFQFPAQVtDVSENAKD  316

         **************************************************99********

S: 240   likklltsreerlgregiedlkkhpff  266

         li++l++sre+rlg++gied+kkhpff

Q: 317   LIRRLICSREHRLGQNGIEDFKKHPFF  343

         **************************9

Domain Sequence
(FASTA)

FEILKVIGRG AFGEVAVVKL KNADKVFAMK ILNKWEMLKR AETACFREER DVLVNGDSKW 60

ITTLHYAFQD DNNLYLVMDY YVGGDLLTLL SKFEDRLPEE MARFYLAEMV IAIDSVHQLH 120

YVHRDIKPDN ILMDMNGHIR LADFGSCLKL MEDGTVQSSV AVGTPDYISP EILQAMEDGK 180

GRYGPECDWW SLGVCMYEML YGETPFYAES LVETYGKIMN HKERFQFPAQ VTDVSENAKD 240

LIRRLICSRE HRLGQNGIED FKKHPFF 267

Keyword

Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Gadus morhua"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0005543	; F:phospholipid binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0005083	; F:small GTPase regulator activity
GO:0031532	; P:actin cytoskeleton reorganization
GO:0035556	; P:intracellular signal transduction
GO:0000226	; P:microtubule cytoskeleton organization
GO:0007097	; P:nuclear migration

KEGG

InterPros

IPR000961	; AGC-kinase_C.
IPR001180	; Citron.
IPR011009	; Kinase-like_dom.
IPR014930	; Myotonic_dystrophy_kinase_coil.
IPR000095	; PAK_box_Rho-bd.
IPR011993	; PH_like_dom.
IPR017892	; Pkinase_C.
IPR001849	; Pleckstrin_homology.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR026611	; Ser/Thr_kinase_MRCK.

Pfam

PF00130	; C1_1; 1.
PF00780	; CNH; 1.
PF08826	; DMPK_coil; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 1.
SM00036	; CNH; 1.
SM00285	; PBD; 1.
SM00233	; PH; 1.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50219	; CNH; 1.
PS50108	; CRIB; 1.
PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 1.
PS50081	; ZF_DAG_PE_2; 1.

Prints

Created Date

20-Feb-2013