EKS-MUM-00181

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00181

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/PKC	435.8	1.5E-130	380	634	255

Status

Reviewed

Ensembl Protein

ENSMUSP00000028118

UniProt Accession

Q02111;

Protein Name

Protein kinase C theta type

Protein Synonyms/Alias

nPKC-theta;

Gene Name

Prkcq

Gene Synonyms/Alias

Prkcq; Pkcq;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000026778	ENSMUSP00000028118	ENSMUST00000028118
ENSMUSG00000026778	ENSMUSP00000110503	ENSMUST00000114853
ENSMUSG00000026778	ENSMUSP00000100035	ENSMUST00000102970

Organism

Mus musculus

Functional Description

Calcium-independent, phospholipid- and diacylglycerol(DAG)-dependent serine/threonine-protein kinase that mediates non- redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non- canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1.

Protein Length

707

Protein Sequence
(FASTA)

MSPFLRIGLS NFDCGTCQAC QGEAVNPYCA VLVKEYVESE NGQMYIQKKP TMYPPWDSTF 60

DAHINKGRVM QIIVKGKNVD LISETTVELY SLAERCRKNN GRTEIWLELK PQGRMLMNAR 120

YFLEMSDTKD MSEFENEGFF ALHQRRGAIK QAKVHHVKCH EFTATFFPQP TFCSVCHEFV 180

WGLNKQGYQC RQCNAAIHKK CIDKVIAKCT GSAINSRETM FHKERFKIDM PHRFKVYNYK 240

SPTFCEHCGT LLWGLARQGL KCDACGMNVH HRCQTKVANL CGINQKLMAE ALAMIESTQQ 300

ARSLRDSEHI FREGPVEIGL PCSTKNETRP PCVPTPGKRE PQGISWDSPL DGSNKSAGPP 360

EPEVSMRRTS LQLKLKIDDF ILHKMLGKGS FGKVFLAEFK RTNQFFAIKA LKKDVVLMDD 420

DVECTMVEKR VLSLAWEHPF LTHMFCTFQT KENLFFVMEY LNGGDLMYHI QSCHKFDLSR 480

ATFYAAEVIL GLQFLHSKGI VYRDLKLDNI LLDRDGHIKI ADFGMCKENM LGDAKTNTFC 540

GTPDYIAPEI LLGQKYNHSV DWWSFGVLVY EMLIGQSPFH GQDEEELFHS IRMDNPFYPR 600

WLEREAKDLL VKLFVREPEK RLGVRGDIRQ HPLFREINWE ELERKEIDPP FRPKVKSPYD 660

CSNFDKEFLS EKPRLSFADR ALINSMDQNM FSNFSFINPG METLICS 707

Nucleotide Sequence
(FASTA)

ATGTCACCGT TTCTTCGAAT CGGTTTATCC AACTTTGACT GTGGGACCTG CCAAGCTTGT 60

CAGGGAGAGG CAGTGAACCC CTACTGCGCT GTGCTTGTCA AAGAGTATGT GGAATCAGAA 120

AATGGGCAGA TGTACATCCA GAAAAAGCCA ACCATGTACC CACCTTGGGA CAGCACCTTT 180

GACGCCCACA TTAACAAGGG AAGGGTGATG CAGATCATCG TGAAGGGCAA GAATGTAGAC 240

CTCATCTCAG AAACAACCGT GGAACTCTAC TCCCTGGCGG AGAGATGCCG CAAGAACAAT 300

GGGCGGACAG AAATATGGTT AGAGCTGAAA CCTCAAGGCC GAATGCTAAT GAATGCAAGA 360

TACTTTCTGG AAATGAGTGA CACAAAGGAC ATGAGTGAGT TTGAGAATGA AGGATTCTTT 420

GCACTGCATC AGCGCCGAGG AGCCATCAAA CAGGCCAAAG TCCACCATGT CAAGTGTCAC 480

GAGTTCACGG CCACCTTTTT CCCTCAACCC ACATTTTGCT CTGTCTGCCA TGAATTTGTC 540

TGGGGCCTGA ACAAGCAGGG TTACCAGTGC CGACAGTGTA ATGCAGCGAT TCACAAGAAG 600

TGCATTGATA AAGTGATAGC CAAGTGCACA GGATCCGCAA TCAATAGCCG AGAAACCATG 660

TTCCATAAGG AGAGATTCAA GATCGACATG CCACACAGAT TCAAAGTCTA CAACTACAAG 720

AGTCCAACCT TCTGTGAGCA CTGTGGTACC CTGCTCTGGG GGCTGGCGAG GCAAGGACTC 780

AAATGTGATG CATGTGGCAT GAACGTCCAC CACCGATGCC AGACAAAGGT TGCCAATCTT 840

TGTGGTATAA ACCAGAAGCT AATGGCTGAA GCACTAGCGA TGATTGAAAG CACCCAACAG 900

GCTCGCTCCT TACGAGATTC AGAACACATC TTCCGAGAAG GCCCAGTTGA AATTGGTCTC 960

CCATGCTCCA CCAAAAACGA AACCAGGCCA CCATGCGTAC CAACACCTGG GAAAAGAGAA 1020

CCCCAGGGCA TTTCCTGGGA TTCCCCTTTG GATGGGTCAA ATAAATCGGC CGGTCCTCCT 1080

GAACCCGAAG TGAGCATGCG CAGGACTTCA CTGCAGCTGA AACTGAAGAT CGATGACTTC 1140

ATCCTGCACA AGATGTTGGG AAAAGGAAGT TTTGGCAAGG TCTTCCTGGC AGAGTTCAAG 1200

AGAACCAATC AGTTTTTCGC AATAAAAGCC TTAAAGAAAG ATGTGGTGTT GATGGATGAT 1260

GACGTCGAGT GTACAATGGT GGAAAAGAGG GTTCTGTCCT TGGCATGGGA GCATCCATTT 1320

CTAACACACA TGTTCTGCAC ATTCCAGACC AAGGAAAATC TCTTTTTCGT GATGGAGTAT 1380

CTCAATGGAG GCGACTTAAT GTACCACATC CAAAGTTGCC ACAAATTTGA TCTTTCCAGA 1440

GCCACGTTTT ATGCTGCTGA GGTCATCCTT GGTCTGCAGT TCCTTCATTC CAAAGGAATT 1500

GTCTACAGGG ACCTGAAGCT TGATAATATC CTGTTAGACA GAGATGGACA TATCAAAATA 1560

GCAGACTTTG GGATGTGCAA AGAGAACATG CTAGGAGATG CGAAGACAAA TACTTTCTGT 1620

GGAACTCCTG ACTACATTGC TCCGGAGATC TTGCTGGGTC AGAAGTACAA CCATTCCGTC 1680

GACTGGTGGT CCTTCGGGGT GCTCGTTTAT GAGATGCTGA TTGGCCAGTC CCCCTTCCAC 1740

GGGCAGGACG AGGAGGAGCT GTTCCACTCC ATCCGCATGG ACAACCCCTT CTACCCGAGG 1800

TGGCTCGAAA GGGAGGCCAA GGACCTTCTA GTGAAGCTTT TTGTGAGAGA ACCTGAGAAG 1860

AGGCTGGGAG TGAGAGGAGA CATCCGCCAG CATCCTTTGT TTCGAGAGAT CAACTGGGAA 1920

GAGCTTGAAA GGAAAGAGAT TGACCCACCC TTCAGACCAA AAGTGAAATC ACCATATGAC 1980

TGTAGCAATT TCGACAAGGA ATTCCTAAGT GAGAAACCCC GGCTATCATT CGCCGACAGA 2040

GCACTCATCA ACAGCATGGA CCAGAACATG TTCAGCAACT TTTCCTTCAT TAACCCAGGG 2100

ATGGAGACTC TCATTTGCTC CTGA 2124

Domain Profile

S: 1     fellkvlGkGsfgkvllaelkktdelyaikvlkkdvvlqdddveltlvekrvlalaskkp  60

         f l+k+lGkGsfgkv+lae k+t++++aik lkkdvvl+dddve+t+vekrvl+la+++p

Q: 380   FILHKMLGKGSFGKVFLAEFKRTNQFFAIKALKKDVVLMDDDVECTMVEKRVLSLAWEHP  439

         7899********************************************************

S: 61    flvqlfscfqtedrlffvleyvnGGdlmfhiqkarklkeerarfyaaeiilalkflhekg  120

         fl+++f++fqt+++lffv+ey+nGGdlm+hiq+++k++ +ra+fyaae+il+l+flh+kg

Q: 440   FLTHMFCTFQTKENLFFVMEYLNGGDLMYHIQSCHKFDLSRATFYAAEVILGLQFLHSKG  499

         ************************************************************

S: 121   iiyrdlkldnvlldaeGhikladfGlckeeilegkttstfcGtPdyiaPeilkeeeygks  180

         i+yrdlkldn+lld++Ghik+adfG+cke++l +++t+tfcGtPdyiaPeil +++y++s

Q: 500   IVYRDLKLDNILLDRDGHIKIADFGMCKENMLGDAKTNTFCGTPDYIAPEILLGQKYNHS  559

         ************************************************************

S: 181   vdwwalGvllyemlagqsPfegededelfesilekevlyPkslskeaveilkglltkdpe  240

         vdww++Gvl+yeml+gqsPf+g+de+elf+si++++++yP++l++ea+++l +l+ ++pe

Q: 560   VDWWSFGVLVYEMLIGQSPFHGQDEEELFHSIRMDNPFYPRWLEREAKDLLVKLFVREPE  619

         ************************************************************

S: 241   krlGvkeegeedikehaff  259

         krlGv  +g  di++h++f

Q: 620   KRLGV--RG--DIRQHPLF  634

         *****..55..89999987

Domain Sequence
(FASTA)

FILHKMLGKG SFGKVFLAEF KRTNQFFAIK ALKKDVVLMD DDVECTMVEK RVLSLAWEHP 60

FLTHMFCTFQ TKENLFFVME YLNGGDLMYH IQSCHKFDLS RATFYAAEVI LGLQFLHSKG 120

IVYRDLKLDN ILLDRDGHIK IADFGMCKEN MLGDAKTNTF CGTPDYIAPE ILLGQKYNHS 180

VDWWSFGVLV YEMLIGQSPF HGQDEEELFH SIRMDNPFYP RWLEREAKDL LVKLFVREPE 240

KRLGVRGDIR QHPLF 255

Keyword

Acetylation; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Immunity; Inflammatory response; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0001772	; C:immunological synapse
GO:0031594	; C:neuromuscular junction
GO:0005634	; C:nucleus
GO:0042383	; C:sarcolemma
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004697	; F:protein kinase C activity
GO:0007568	; P:aging
GO:0006954	; P:inflammatory response
GO:0035556	; P:intracellular signal transduction
GO:0006509	; P:membrane protein ectodomain proteolysis
GO:0046627	; P:negative regulation of insulin receptor signaling pathway
GO:0070233	; P:negative regulation of T cell apoptotic process
GO:0030182	; P:neuron differentiation
GO:0051491	; P:positive regulation of filopodium assembly
GO:0032740	; P:positive regulation of interleukin-17 production
GO:0045086	; P:positive regulation of interleukin-2 biosynthetic process
GO:0032753	; P:positive regulation of interleukin-4 production
GO:0042346	; P:positive regulation of NF-kappaB import into nucleus
GO:0051092	; P:positive regulation of NF-kappaB transcription factor activity
GO:0050714	; P:positive regulation of protein secretion
GO:0051496	; P:positive regulation of stress fiber assembly
GO:0042102	; P:positive regulation of T cell proliferation
GO:2000318	; P:positive regulation of T-helper 17 type immune response
GO:2000570	; P:positive regulation of T-helper 2 cell activation
GO:0010389	; P:regulation of G2/M transition of mitotic cell cycle
GO:0090330	; P:regulation of platelet aggregation
GO:0019229	; P:regulation of vasoconstriction
GO:0045730	; P:respiratory burst
GO:0009749	; P:response to glucose stimulus
GO:0009408	; P:response to heat
GO:0001666	; P:response to hypoxia
GO:0032868	; P:response to insulin stimulus
GO:0014070	; P:response to organic cyclic compound
GO:0042246	; P:tissue regeneration

KEGG

mmu:18761

;

InterPros

IPR000961	; AGC-kinase_C.
IPR008973	; C2_Ca/lipid-bd_dom_CaLB.
IPR020454	; DAG/PE-bd.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR014376	; Prot_kin_PKC_delta.
IPR002219	; Prot_Kinase_C-like_PE/DAG-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00130	; C1_1; 2.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00109	; C1; 2.
SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS50004	; C2; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS00479	; ZF_DAG_PE_1; 2.
PS50081	; ZF_DAG_PE_2; 2.

Prints

PR00008

; DAGPEDOMAIN.

Created Date

20-Feb-2013