EKS-MUM-00322

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00322

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/BUB	383.6	1.0E-114	763	1026	264

Status

Unreviewed

Ensembl Protein

ENSMUSP00000028858

UniProt Accession

A2APR8;

Protein Name

Protein Synonyms/Alias

Budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae); Mitotic checkpoint serine/threonine-protein kinase BUB1;

Gene Name

Bub1

Gene Synonyms/Alias

Bub1; RP23-189N15.7-001;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000027379	ENSMUSP00000028858	ENSMUST00000028858

Organism

Mus musculus

Functional Description

Protein Length

1059

Protein Sequence
(FASTA)

MDNLENVFRM FEAHMQSYTG NDPLGEWESF IKWVEENFPD NKEYLMTLLE HLMKEFLHKK 60

NYHNDSRFIN YCLKFAEYNS DRHQFFEFLY NQGIGTKSSY IYMSWAGHLE AQGELQHASA 120

IFQTGIHNEA EPKELLQQQY RLFQARLTGI HLPAQATTSE PLHSAQILNQ VMMTNSSPEK 180

NSACVPRSQG SECSGVASST CDEKSNIREQ RVIMISKSEC SVSSSVAPKP EAQQVMYCKE 240

KLIRGDSEFS FEELRAQKYN QRKKHEQWVS EDRNYMKRKE ANAFEEQLLK QKMDELHKKL 300

HQVVELSHKD LPASENRPDV SLVCVGQNTC SQQELRGPSL SSISHQTSES SGEKPQEEPS 360

VPLMVNAVNS TLLFPAANLP ALPVPVSGQS LTDSRCVNQS VHEFMPQCGP ETKEVCETNK 420

VASINDFHTT PNTSLGMVQG TPCKVQPSPT VHTKEALGFI MDMFQAPTLP DISDDKDEWP 480

SLDQNEDAFE AQFQKNAVSS GDWGVKKIMT LSSAFPIFED GNKENYGLPQ PKNKPLGART 540

FGERSLSKYS SRSNEMPHTD EFMDDSTVCG IRCNKTLAPS PKSIGDFTSA AQLSSTPFHK 600

FPADLVQIPE DKENVVATQY THMALDSCKE NIVDLSKGRK LGPIQEKISA SLPCPSQPAT 660

GGLFTQEAVF GLEAFKCTGI DHATVEDLSD ANAGLQVECV QTLGNVNAPS FTVENPWDDE 720

LILKLLSGLS KPVTSYSNTF EWQSKLPAIK TKTEYQLGSL LVYVNHLLGE GAFAQVFEAI 780

HGDVRNAKSE QKCILKVQRP ANSWEFYIGM QLMERLKPEV HHMFIKFYSA HLFKNGSILV 840

GELYSYGTLL NVINLYKNTS EKVMPQALVL TFAIRMLYMV EQVHSCEIIH GDIKPDNFIL 900

GHRFLEQADE DLATGLALID LGQSIDMKLF PKGTVFTGKC ETSGFQCPEM LSNKPWNYQI 960

DYFGVAATIY CMLFGSYMKV KNEGGVWKPE GLFRRLPHLD MWEEFFHIML NIPDCHNLPS 1020

LDFLRQNMKK LLEQQYSNKI KTLRNRLIVM LSEYKRSRK 1059

Nucleotide Sequence
(FASTA)

ATGGACAACC TAGAAAATGT CTTTCGCATG TTTGAAGCCC ATATGCAAAG CTACACGGGT 60

AATGACCCAC TTGGAGAATG GGAAAGCTTT ATAAAGTGGG TAGAAGAGAA TTTTCCTGAC 120

AATAAAGAAT ACTTGATGAC ATTATTAGAA CATTTAATGA AGGAATTTTT ACATAAGAAG 180

AACTACCACA ATGATTCAAG ATTCATCAAT TATTGCTTAA AATTTGCTGA GTACAACAGT 240

GACCGTCATC AGTTTTTTGA GTTTCTGTAC AACCAGGGAA TTGGAACCAA GTCATCATAT 300

ATATACATGT CCTGGGCAGG GCATCTGGAA GCCCAGGGAG AGCTGCAGCA TGCCAGTGCT 360

ATTTTTCAGA CAGGAATTCA CAATGAGGCT GAACCTAAAG AACTACTACA GCAACAATAC 420

AGGCTATTCC AAGCACGCCT TACTGGAATC CATTTGCCAG CTCAAGCTAC AACCTCAGAA 480

CCTTTGCATA GTGCACAGAT TTTAAACCAA GTTATGATGA CAAACTCAAG TCCAGAAAAA 540

AACTCAGCCT GTGTTCCTAG GAGTCAGGGT TCAGAATGTT CTGGTGTGGC ATCTTCCACT 600

TGTGATGAAA AGTCTAATAT TAGGGAACAA AGGGTGATCA TGATTTCCAA GTCAGAATGC 660

TCTGTCAGCT CATCTGTGGC ACCCAAGCCT GAGGCTCAGC AAGTTATGTA CTGCAAGGAA 720

AAGCTTATTC GTGGAGATTC AGAATTTTCT TTTGAAGAAC TGAGAGCCCA GAAATATAAT 780

CAAAGGAAGA AGCATGAGCA GTGGGTTAGT GAAGACAGAA ATTATATGAA AAGGAAAGAA 840

GCAAATGCTT TTGAAGAGCA ATTATTAAAA CAGAAAATGG ATGAACTTCA CAAGAAATTG 900

CATCAAGTGG TGGAATTGTC ACACAAGGAC CTTCCTGCTT CTGAGAACAG GCCTGATGTT 960

AGTCTAGTAT GTGTTGGACA AAATACTTGC TCCCAGCAGG AATTGAGGGG TCCAAGTCTT 1020

TCATCCATCA GTCATCAGAC CTCAGAGAGT TCAGGAGAGA AACCACAGGA AGAACCTTCT 1080

GTTCCTCTTA TGGTAAATGC TGTTAACAGC ACTTTGCTGT TCCCAGCTGC CAACCTGCCA 1140

GCTCTTCCTG TTCCTGTAAG TGGCCAGTCA TTGACAGACT CCAGATGTGT GAATCAAAGT 1200

GTTCATGAAT TCATGCCACA GTGTGGACCA GAAACAAAAG AAGTGTGTGA AACAAATAAA 1260

GTTGCCAGCA TTAATGATTT TCATACAACT CCAAACACAT CATTGGGAAT GGTTCAAGGA 1320

ACACCATGCA AAGTGCAGCC ATCACCAACT GTCCACACCA AGGAAGCATT AGGTTTCATC 1380

ATGGACATGT TTCAGGCTCC AACACTTCCT GACATTTCTG ATGATAAAGA TGAATGGCCA 1440

TCTCTGGACC AAAATGAAGA TGCATTTGAA GCCCAGTTTC AAAAAAATGC AGTATCTTCG 1500

GGAGATTGGG GAGTTAAAAA AATTATGACT TTGTCATCTG CTTTTCCTAT TTTTGAAGAT 1560

GGAAACAAAG AAAATTATGG CTTACCACAG CCTAAAAATA AGCCCTTAGG AGCTAGGACC 1620

TTTGGAGAAC GATCTCTCAG TAAATATTCC TCGAGATCAA ATGAAATGCC TCACACTGAT 1680

GAGTTTATGG ATGATTCAAC AGTATGTGGT ATTCGCTGCA ACAAAACTCT AGCTCCCAGT 1740

CCTAAAAGTA TAGGAGACTT TACATCTGCT GCCCAACTTT CGTCTACACC ATTCCACAAA 1800

TTTCCAGCAG ATTTAGTACA GATTCCAGAA GATAAAGAAA ATGTGGTAGC CACACAGTAT 1860

ACACATATGG CTTTGGATTC TTGTAAAGAA AACATAGTGG ACCTCTCAAA AGGCAGAAAG 1920

CTTGGGCCAA TTCAAGAGAA AATTTCAGCA TCTTTACCCT GTCCTAGTCA GCCTGCCACA 1980

GGTGGTTTGT TCACCCAGGA AGCAGTGTTC GGCCTTGAGG CTTTTAAATG CACAGGCATT 2040

GACCATGCGA CAGTGGAAGA CCTATCCGAT GCCAATGCTG GGCTCCAAGT TGAATGCGTG 2100

CAGACACTTG GAAATGTCAA TGCTCCAAGC TTTACTGTTG AGAACCCATG GGATGACGAA 2160

TTGATTCTTA AACTTCTCTC TGGACTTTCT AAGCCAGTTA CTTCCTATTC AAATACTTTT 2220

GAGTGGCAGA GTAAACTTCC AGCCATCAAG ACCAAGACAG AATATCAATT GGGTTCTTTG 2280

CTGGTCTATG TGAATCACCT TCTTGGAGAA GGAGCCTTTG CTCAAGTCTT TGAAGCTATT 2340

CATGGAGATG TGAGAAATGC CAAAAGTGAA CAGAAATGCA TTTTGAAGGT GCAGAGACCT 2400

GCCAACTCCT GGGAATTCTA CATTGGGATG CAGCTGATGG AAAGACTAAA GCCAGAAGTA 2460

CATCACATGT TCATCAAGTT TTATTCTGCT CATTTATTCA AGAATGGCAG CATATTAGTA 2520

GGGGAACTCT ACAGCTATGG GACGTTACTA AATGTCATTA ACCTCTATAA AAATACCTCT 2580

GAAAAAGTGA TGCCCCAGGC TCTTGTCCTC ACTTTCGCTA TCAGAATGCT TTACATGGTT 2640

GAACAAGTCC ACAGCTGCGA AATCATTCAT GGAGACATTA AGCCAGATAA CTTCATACTA 2700

GGACACAGAT TTTTGGAACA GGCTGATGAA GACTTAGCTA CCGGCTTGGC ATTGATTGAC 2760

CTGGGTCAGA GTATAGATAT GAAACTTTTC CCTAAAGGAA CTGTATTTAC AGGAAAATGT 2820

GAAACATCTG GTTTTCAGTG TCCTGAGATG CTCAGTAACA AGCCATGGAA CTACCAGATT 2880

GATTACTTTG GAGTTGCTGC AACAATATAC TGTATGCTCT TTGGCTCTTA CATGAAAGTA 2940

AAAAATGAAG GAGGAGTCTG GAAACCTGAA GGTCTTTTTA GAAGGCTTCC TCATTTGGAT 3000

ATGTGGGAGG AATTTTTTCA CATCATGTTG AATATACCGG ATTGTCATAA TCTTCCATCT 3060

TTGGATTTTC TGAGACAGAA TATGAAGAAA TTACTTGAAC AACAGTATTC CAACAAGATT 3120

AAGACCTTGC GTAATAGGCT AATTGTGATG CTTTCAGAAT ATAAGCGTTC AAGAAAATAA 3180

Domain Profile

S: 2     eiekeigeGayakvykaeh....dsksgelvalKvekpansWefYIclqlkeRlkeeell  57

         ++++++geGa+a+v++a h    ++ks+++++lKv++pansWefYI +ql+eRlk +e++

Q: 763   YVNHLLGEGAFAQVFEAIHgdvrNAKSEQKCILKVQRPANSWEFYIGMQLMERLK-PEVH  821

         79******************999999*****************************.88**

S: 58    ksiieissallfqdasllvleyasygtlldlinkvkevtnkvmdeelvvllivqllkive  117

         +++i+++sa+lf+++s+lv e++sygtll++in++k++++kvm+++lv+++++++l++ve

Q: 822   HMFIKFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVE  881

         ************************************************************

S: 118   klHeveIiHgDlkPdnfllgnrimdkadkdkeekglkliDfgrsiDmkllpdgtkfkgka  177

         ++H++eIiHgD+kPdnf+lg+r+ ++ad ++ ++gl+liD+g+siDmkl+p+gt f+gk+

Q: 882   QVHSCEIIHGDIKPDNFILGHRFLEQAD-EDLATGLALIDLGQSIDMKLFPKGTVFTGKC  940

         **************************99.77899**************************

S: 178   ktdafeciemlagkswsYqvDlfglaavihvllfgkyleviqeggswklkqkikrllkke  237

         +t++f+c+eml++k+w+Yq+D+fg+aa+i+++lfg+y++v++egg wk++  ++rl++ +

Q: 941   ETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKVKNEGGVWKPEGLFRRLPHLD  1000

         ************************************************************

S: 238   lwekiferlLNisdastlpsleelve  263

         +we++f+ +LNi+d+++lpsl+ l++

Q: 1001  MWEEFFHIMLNIPDCHNLPSLDFLRQ  1026

         ************************96

Domain Sequence
(FASTA)

YVNHLLGEGA FAQVFEAIHG DVRNAKSEQK CILKVQRPAN SWEFYIGMQL MERLKPEVHH 60

MFIKFYSAHL FKNGSILVGE LYSYGTLLNV INLYKNTSEK VMPQALVLTF AIRMLYMVEQ 120

VHSCEIIHGD IKPDNFILGH RFLEQADEDL ATGLALIDLG QSIDMKLFPK GTVFTGKCET 180

SGFQCPEMLS NKPWNYQIDY FGVAATIYCM LFGSYMKVKN EGGVWKPEGL FRRLPHLDMW 240

EEFFHIMLNI PDCHNLPSLD FLRQ 264

Keyword

Complete proteome; Reference proteome.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0000777	; C:condensed chromosome kinetochore
GO:0000780	; C:condensed nuclear chromosome, centromeric region
GO:0000776	; C:kinetochore
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0008283	; P:cell proliferation
GO:0007063	; P:regulation of sister chromatid cohesion
GO:0071173	; P:spindle assembly checkpoint

KEGG

mmu:12235

;

InterPros

IPR011009	; Kinase-like_dom.
IPR013212	; Mad3_BUB1_I.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR015661	; Ser/Thr_kinase_Bub1.

Pfam

PF08311	; Mad3_BUB1_I; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00777

; Mad3_BUB1_I; 1.

Prosites

PS51489	; BUB1_N; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013