EKS-MUM-00415

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00415

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	315.1	8.7E-94	36	283	248

Status

Reviewed

Ensembl Protein

ENSMUSP00000103818

UniProt Accession

Q62073;

Protein Name

Mitogen-activated protein kinase kinase kinase 7

Protein Synonyms/Alias

Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1;

Gene Name

Map3k7

Gene Synonyms/Alias

Map3k7; Tak1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000028284	ENSMUSP00000079734	ENSMUST00000080933
ENSMUSG00000028284	ENSMUSP00000103818	ENSMUST00000108183
ENSMUSG00000028284	ENSMUSP00000103819	ENSMUST00000108184
ENSMUSG00000028284	ENSMUSP00000040307	ENSMUST00000037607

Organism

Mus musculus

Functional Description

Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2- induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B.

Protein Length

491

Protein Sequence
(FASTA)

MSTASAASSS SSSSASEMIE APSQVLNFEE IDYKEIEVEE VVGRGAFGVV CKAKWRAKDV 60

AIKQIESESE RKAFIVELRQ LSRVNHPNIV KLYGACLNPV CLVMEYAEGG SLYNVLHGAE 120

PLPYYTAAHA MSWCLQCSQG VAYLHSMQPK ALIHRDLKPP NLLLVAGGTV LKICDFGTAC 180

DIQTHMTNNK GSAAWMAPEV FEGSNYSEKC DVFSWGIILW EVITRRKPFD EIGGPAFRIM 240

WAVHNGTRPP LIKNLPKPIE SLMTRCWSKD PSQRPSMEEI VKIMTHLMRY FPGADEPLQY 300

PCQYSDEGQS NSATSTGSFM DIASTNTSNK SDTNMEQVPA TNDTIKRLES KLLKNQAKQQ 360

SESGRLSLGA SRGSSVESLP PTSEGKRMSA DMSEIEARIV ATAGNGQPRR RSIQDLTVTG 420

TEPGQVSSRS SSPSVRMITT SGPTSEKPAR SHPWTPDDST DTNGSDNSIP MAYLTLDHQL 480

QARTSCRIGP G 491

Nucleotide Sequence
(FASTA)

ATGTCGACAG CCTCCGCCGC CTCGTCCTCC TCCTCGTCTT CTGCCAGTGA GATGATCGAA 60

GCGCCGTCGC AGGTCCTGAA CTTCGAAGAG ATCGACTACA AGGAGATCGA GGTGGAAGAG 120

GTTGTCGGAA GAGGAGCTTT TGGAGTAGTT TGCAAAGCTA AGTGGAGAGC AAAAGATGTC 180

GCTATTAAAC AGATAGAAAG TGAGTCTGAG AGGAAGGCTT TCATTGTGGA GCTCCGGCAG 240

TTGTCACGTG TGAACCATCC TAACATTGTC AAGTTGTATG GAGCCTGCCT GAATCCAGTA 300

TGTCTTGTGA TGGAATATGC AGAGGGGGGC TCATTGTATA ATGTGCTGCA TGGTGCTGAA 360

CCATTGCCTT ACTACACTGC TGCTCATGCC ATGAGCTGGT GTTTACAGTG TTCCCAAGGA 420

GTGGCTTACC TGCACAGCAT GCAGCCCAAA GCGCTGATTC ACAGGGACCT CAAGCCTCCA 480

AACTTGCTGC TGGTTGCAGG AGGGACAGTT CTAAAAATCT GCGATTTTGG TACAGCTTGT 540

GACATCCAAA CACACATGAC CAATAATAAA GGGAGTGCTG CTTGGATGGC GCCTGAAGTA 600

TTTGAAGGTA GCAATTACAG TGAAAAGTGT GATGTCTTCA GCTGGGGTAT TATCCTCTGG 660

GAAGTGATAA CACGCCGGAA ACCCTTCGAT GAGATCGGTG GCCCAGCTTT CAGAATCATG 720

TGGGCTGTTC ATAATGGCAC TCGACCACCA CTGATCAAAA ATTTACCTAA GCCCATTGAG 780

AGCTTGATGA CACGCTGTTG GTCTAAGGAC CCATCTCAGC GCCCTTCAAT GGAGGAAATT 840

GTGAAAATAA TGACTCACTT GATGCGGTAC TTCCCAGGAG CGGATGAGCC GTTACAGTAT 900

CCTTGTCAGT ACTCTGATGA AGGGCAGAGC AACTCAGCCA CCAGCACAGG CTCATTCATG 960

GACATTGCTT CTACAAATAC CAGTAATAAA AGTGACACAA ATATGGAACA GGTTCCTGCC 1020

ACAAACGACA CTATTAAACG CTTGGAGTCA AAACTTTTGA AAAACCAGGC AAAGCAACAG 1080

AGTGAATCTG GACGCCTGAG CTTGGGAGCC TCTCGTGGGA GCAGTGTGGA GAGCTTGCCC 1140

CCCACTTCCG AGGGCAAGAG GATGAGTGCT GACATGTCTG AAATAGAAGC CAGGATCGTG 1200

GCGACTGCAG GTAACGGGCA ACCAAGGCGT AGATCCATCC AAGACTTGAC TGTTACTGGG 1260

ACAGAACCTG GTCAGGTGAG CAGCCGGTCA TCCAGCCCTA GTGTCAGAAT GATCACTACC 1320

TCAGGACCAA CCTCAGAGAA GCCAGCTCGC AGTCACCCGT GGACCCCTGA TGATTCCACA 1380

GATACCAATG GCTCAGATAA CTCCATCCCA ATGGCGTATC TTACACTGGA TCACCAGCTA 1440

CAGGCAAGAA CTAGTTGCAG AATTGGACCA GGATGA 1476

Domain Profile

S: 1     ieleeligsgafgkvykakwrkkevavKklreeaeekekleklereakllsklshpniie  60

         ie+ee++g+gafg v+kakwr+k+va+K++++e+e+    +++  e+++ls+++hpni++

Q: 36    IEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESER----KAFIVELRQLSRVNHPNIVK  91

         5789****************************9888....********************

S: 61    llGvcleepnlllvmeyveggsLsnvlhe.kskreidlevlvswalqiArGmaYLhseap  119

         l+G+cl++  ++lvmey+eggsL+nvlh+ +  + ++ ++++sw+lq+++G+aYLhs++p

Q: 92    LYGACLNP--VCLVMEYAEGGSLYNVLHGaEPLPYYTAAHAMSWCLQCSQGVAYLHSMQP  149

         *******9..89*****************778899*************************

S: 120   kkiihrdLksknvLlseekktlkicdFGtarelskstkmsaagtlawmaPEvlkeekvse  179

         k++ihrdLk++n+Ll +  ++lkicdFGta++++ +++++++g++awmaPEv++ +++se

Q: 150   KALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQ-THMTNNKGSAAWMAPEVFEGSNYSE  208

         ********************************99.9************************

S: 180   ksDvwSygvvLWelltrevPykdlse..laiaykvalkglrlpipsscpeklkkLmkecw  237

         k+Dv+S+g++LWe++tr++P++++    ++i+++v ++g+r+p+++++p+++++Lm++cw

Q: 209   KCDVFSWGIILWEVITRRKPFDEIGGpaFRIMWAV-HNGTRPPLIKNLPKPIESLMTRCW  267

         **********************9987447889999.99**********************

S: 238   nedpekRpsfseivks  253

         ++dp++Rps++eivk+

Q: 268   SKDPSQRPSMEEIVKI  283

         *************986

Domain Sequence
(FASTA)

IEVEEVVGRG AFGVVCKAKW RAKDVAIKQI ESESERKAFI VELRQLSRVN HPNIVKLYGA 60

CLNPVCLVME YAEGGSLYNV LHGAEPLPYY TAAHAMSWCL QCSQGVAYLH SMQPKALIHR 120

DLKPPNLLLV AGGTVLKICD FGTACDIQTH MTNNKGSAAW MAPEVFEGSN YSEKCDVFSW 180

GIILWEVITR RKPFDEIGGP AFRIMWAVHN GTRPPLIKNL PKPIESLMTR CWSKDPSQRP 240

SMEEIVKI 248

Keyword

Apoptosis; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transcription; Transcription regulation; Transferase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004708	; F:MAP kinase kinase activity
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0001525	; P:angiogenesis
GO:0006915	; P:apoptotic process
GO:0007252	; P:I-kappaB phosphorylation
GO:0043066	; P:negative regulation of apoptotic process
GO:0060546	; P:negative regulation of necroptosis
GO:2000378	; P:negative regulation of reactive oxygen species metabolic process
GO:0001841	; P:neural tube formation
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0046330	; P:positive regulation of JNK cascade
GO:0043507	; P:positive regulation of JUN kinase activity
GO:0006355	; P:regulation of transcription, DNA-dependent
GO:0006950	; P:response to stress
GO:0006351	; P:transcription, DNA-dependent
GO:0007179	; P:transforming growth factor beta receptor signaling pathway

KEGG

mmu:26409

;

InterPros

IPR011009	; Kinase-like_dom.
IPR017421	; MAPKKK7.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF07714

; Pkinase_Tyr; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013