EKS-MUM-00004

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00004

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Eph	516.6	2.1E-155	633	889	257

Status

Reviewed

Ensembl Protein

ENSMUSP00000029964

UniProt Accession

Q61772; Q61505; Q61773; Q61774; Q8BSU8;

Protein Name

Ephrin type-A receptor 7

Protein Synonyms/Alias

Developmental kinase 1; mDK-1; EPH homology kinase 3; EHK-3; Embryonic brain kinase; EBK;

Gene Name

Epha7

Gene Synonyms/Alias

Epha7; Ebk, Ehk3, Mdk1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000028289	ENSMUSP00000029964	ENSMUST00000029964
ENSMUSG00000028289	ENSMUSP00000103829	ENSMUST00000108194
ENSMUSG00000028289	ENSMUSP00000079735	ENSMUST00000080934
ENSMUSG00000028289	ENSMUSP00000103826	ENSMUST00000108191

Organism

Mus musculus

Functional Description

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4 which lacks the kinase domain may regulate isoform 1 adhesive properties.

Protein Length

998

Protein Sequence
(FASTA)

MVVQTRFPSW IILCYIWLLG FAHTGEAQAA KEVLLLDSKA QQTELEWISS PPSGWEEISG 60

LDENYTPIRT YQVCQVMEPN QNNWLRTNWI SKGNAQRIFV ELKFTLRDCN SLPGVLGTCK 120

ETFNLYYYET DYDTGRNIRE NLYVKIDTIA ADESFTQGDL GERKMKLNTE VREIGPLSKK 180

GFYLAFQDVG ACIALVSVKV YYKKCWSIVE NLAVFPDTVT GSEFSSLVEV RGTCVSSAEE 240

EAENSPRMHC SAEGEWLVPI GKCICKAGYQ QKGDTCEPCG RRFYKSSSQD LQCSRCPTHS 300

FSDREGSSRC ECEDGYYRAP SDPPYVACTR PPSAPQNLIF NINQTTVSLE WSPPADNGGR 360

NDVTYRILCK RCSWEQGECV PCGSNIGYMP QQTGLEDNYV TVMDLLAHAN YTFEVEAVNG 420

VSDLSRSQRL FAAVSITTGQ AAPSQVSGVM KERVLQRSVQ LSWQEPEHPN GVITEYEIKY 480

YEKDQRERTY STLKTKSTSA SINNLKPGTV YVFQIRAVTA AGYGNYSPRL DVATLEEASG 540

KMFEATAVSS EQNPVIIIAV VAVAGTIILV FMVFGFIIGR RHCGYSKADQ EGDEELYFHF 600

KFPGTKTYID PETYEDPNRA VHQFAKELDA SCIKIERVIG AGEFGEVCSG RLKLPGKRDV 660

AVAIKTLKVG YTEKQRRDFL CEASIMGQFD HPNVVHLEGV VTRGKPVMIV IEFMENGALD 720

AFLRKHDGQF TVIQLVGMLR GIAAGMRYLA DMGYVHRDLA ARNILVNSNL VCKVSDFGLS 780

RVIEDDPEAV YTTTGGKIPV RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM 840

SNQDVIKAIE EGYRLPAPMD CPAGLHQLML DCWQKDRAER PKFEQIVGIL DKMIRNPSSL 900

KTPLGTCSRP LSPLLDQSTP DFTAFCSVGE WLQAIKMERY KDNFTAAGYN SLESVARMTI 960

DDVMSLGITL VGHQKKIMSS IQTMRAQMLH LHGTGIQV 998

Nucleotide Sequence
(FASTA)

ATGGTTGTTC AAACTCGGTT CCCTTCGTGG ATTATTTTGT GTTACATCTG GCTGCTTGGC 60

TTTGCACACA CGGGGGAGGC GCAGGCTGCG AAGGAAGTAC TATTACTGGA CTCGAAAGCA 120

CAACAAACAG AATTGGAATG GATTTCCTCT CCACCCAGTG GGTGGGAAGA AATTAGTGGT 180

TTGGATGAGA ACTACACTCC GATAAGAACA TACCAGGTGT GCCAGGTCAT GGAGCCCAAC 240

CAGAACAACT GGCTGCGGAC TAACTGGATT TCTAAAGGCA ACGCACAAAG GATTTTTGTA 300

GAATTGAAAT TCACCTTGAG GGATTGTAAT AGTCTTCCCG GAGTCCTGGG AACTTGCAAG 360

GAAACGTTTA ATTTGTACTA TTATGAAACA GACTACGACA CCGGCAGGAA TATACGAGAA 420

AACCTTTATG TTAAAATAGA CACCATTGCT GCAGATGAAA GTTTCACACA AGGTGACCTT 480

GGTGAAAGAA AGATGAAGCT GAACACTGAG GTGAGAGAGA TTGGACCTTT GTCCAAAAAG 540

GGATTCTATC TTGCCTTTCA GGATGTAGGG GCTTGCATAG CATTGGTTTC TGTCAAAGTG 600

TACTACAAGA AGTGCTGGTC CATTGTTGAG AACTTAGCTG TCTTTCCAGA TACAGTGACT 660

GGTTCGGAAT TTTCCTCCTT AGTCGAGGTC CGTGGGACAT GTGTCAGCAG TGCCGAGGAA 720

GAGGCAGAAA ATTCCCCCAG AATGCATTGC AGTGCAGAAG GAGAGTGGCT AGTACCCATT 780

GGAAAATGCA TCTGCAAAGC AGGCTATCAG CAAAAAGGGG ACACTTGCGA ACCCTGTGGC 840

CGCAGGTTCT ACAAATCTTC CTCTCAGGAT CTCCAGTGTT CTCGTTGTCC AACCCACAGC 900

TTCTCTGACC GAGAAGGATC ATCCAGGTGT GAATGTGAAG ATGGGTACTA CAGAGCTCCT 960

TCTGATCCAC CATACGTTGC ATGCACGAGG CCTCCCTCTG CACCACAGAA CCTTATTTTC 1020

AATATCAATC AAACGACTGT AAGTTTGGAA TGGAGTCCTC CGGCTGACAA CGGGGGAAGA 1080

AACGATGTCA CCTACAGAAT ACTGTGTAAG CGGTGCAGTT GGGAACAGGG AGAATGTGTG 1140

CCATGCGGAA GTAACATTGG ATACATGCCC CAGCAGACGG GATTAGAGGA TAACTATGTC 1200

ACTGTCATGG ACCTACTTGC CCATGCAAAT TACACTTTCG AAGTTGAAGC TGTAAATGGA 1260

GTTTCGGACT TAAGCAGATC CCAGAGGCTC TTCGCTGCTG TTAGCATCAC CACCGGTCAA 1320

GCAGCTCCCT CGCAAGTGAG TGGAGTCATG AAGGAGCGAG TACTGCAGCG GAGTGTGCAG 1380

CTTTCCTGGC AGGAGCCGGA GCATCCCAAT GGAGTCATCA CGGAATATGA AATCAAGTAT 1440

TATGAGAAAG ATCAACGGGA AAGGACGTAC TCAACACTCA AAACCAAGTC CACCTCCGCC 1500

TCCATTAATA ATCTGAAACC GGGAACAGTG TACGTCTTTC AGATCCGGGC GGTCACTGCT 1560

GCCGGTTATG GAAACTACAG CCCTAGGCTT GATGTTGCCA CACTTGAGGA AGCTTCAGGT 1620

AAAATGTTTG AAGCGACAGC AGTCTCCAGT GAACAGAATC CTGTCATCAT AATTGCTGTA 1680

GTGGCTGTAG CAGGGACCAT CATCTTGGTG TTCATGGTGT TCGGCTTCAT CATTGGAAGA 1740

AGGCACTGTG GTTATAGCAA GGCTGACCAA GAAGGGGATG AAGAACTCTA CTTTCATTTT 1800

AAATTTCCAG GCACCAAAAC CTACATTGAC CCTGAAACCT ATGAGGACCC AAATAGAGCT 1860

GTCCATCAAT TCGCCAAGGA GCTAGATGCC TCCTGTATTA AAATTGAGCG TGTGATTGGT 1920

GCAGGAGAAT TTGGAGAAGT TTGCAGTGGT CGTTTGAAAC TTCCGGGGAA GAGAGATGTT 1980

GCAGTGGCCA TAAAAACCCT GAAAGTTGGT TACACAGAAA AGCAAAGGAG GGACTTTTTA 2040

TGCGAAGCAA GCATCATGGG GCAATTTGAC CACCCAAATG TCGTCCATTT GGAAGGGGTT 2100

GTTACAAGAG GGAAGCCTGT CATGATTGTG ATAGAGTTCA TGGAGAATGG AGCCCTGGAT 2160

GCATTTCTCA GGAAACACGA TGGGCAGTTT ACAGTCATTC AGTTGGTAGG AATGTTGAGA 2220

GGTATTGCCG CTGGGATGCG ATACTTGGCT GATATGGGAT ACGTTCACAG GGACCTTGCA 2280

GCGCGCAACA TCCTTGTCAA CAGCAATCTT GTTTGTAAAG TGTCAGATTT TGGCCTTTCC 2340

CGGGTTATAG AGGATGATCC CGAAGCTGTC TACACCACGA CTGGTGGAAA AATTCCAGTA 2400

AGGTGGACTG CACCGGAAGC CATTCAATAC CGGAAGTTCA CCTCAGCCAG CGATGTGTGG 2460

AGCTATGGGA TTGTCATGTG GGAAGTGATG TCTTATGGAG AAAGACCTTA CTGGGACATG 2520

TCAAATCAAG ATGTCATTAA AGCGATAGAA GAAGGTTATC GTTTGCCGGC GCCCATGGAT 2580

TGCCCAGCTG GTCTTCACCA GCTAATGCTG GATTGTTGGC AGAAAGATCG GGCGGAAAGG 2640

CCAAAGTTTG AGCAGATAGT CGGAATTCTA GACAAAATGA TTCGAAACCC AAGTAGTCTG 2700

AAAACACCCC TGGGAACTTG TAGTAGACCC TTAAGCCCTC TTCTGGACCA GAGCACTCCT 2760

GACTTCACTG CCTTCTGTTC AGTTGGAGAA TGGTTGCAAG CTATTAAAAT GGAAAGGTAT 2820

AAGGACAACT TCACAGCAGC GGGTTACAAC TCACTCGAGT CAGTGGCCAG GATGACTATC 2880

GATGATGTGA TGAGTTTAGG GATCACACTG GTTGGCCATC AAAAGAAGAT CATGAGCAGC 2940

ATCCAGACTA TGCGGGCACA AATGTTGCAT TTACACGGAA CAGGCATCCA AGTGTGA 2997

Domain Profile

S: 1     vkiekviGaGefGevcsGrlklpgkreiavaiktlkagstekqrrdflseasilGqfdhp  60

         +kie+viGaGefGevcsGrlklpgkr++avaiktlk g+tekqrrdfl+easi+Gqfdhp

Q: 633   IKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHP  692

         79**********************************************************

S: 61    niirleGvvtkskpvmivteymengaldsflrkkdgqftviqlvgmlrGiasgmkylsdm  120

         n+++leGvvt++kpvmiv+e+mengald+flrk+dgqftviqlvgmlrGia+gm+yl+dm

Q: 693   NVVHLEGVVTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADM  752

         ************************************************************

S: 121   gyvhrdlaarnilvnsnlvckvsdfGlsrvleddseaayttsggkipirwtapeaiayrk  180

         gyvhrdlaarnilvnsnlvckvsdfGlsrv+edd+ea+ytt+ggkip+rwtapeai+yrk

Q: 753   GYVHRDLAARNILVNSNLVCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRK  812

         ************************************************************

S: 181   ftsasdvwsyGivlwevlsyGerpywdmsnqdvikaieegyrlpppmdcpaalyqlmldc  240

         ftsasdvwsyGiv+wev+syGerpywdmsnqdvikaieegyrlp+pmdcpa+l+qlmldc

Q: 813   FTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDC  872

         ************************************************************

S: 241   wqkdraerpkfeqivsi  257

         wqkdraerpkfeqiv i

Q: 873   WQKDRAERPKFEQIVGI  889

         **************987

Domain Sequence
(FASTA)

IKIERVIGAG EFGEVCSGRL KLPGKRDVAV AIKTLKVGYT EKQRRDFLCE ASIMGQFDHP 60

NVVHLEGVVT RGKPVMIVIE FMENGALDAF LRKHDGQFTV IQLVGMLRGI AAGMRYLADM 120

GYVHRDLAAR NILVNSNLVC KVSDFGLSRV IEDDPEAVYT TTGGKIPVRW TAPEAIQYRK 180

FTSASDVWSY GIVMWEVMSY GERPYWDMSN QDVIKAIEEG YRLPAPMDCP AGLHQLMLDC 240

WQKDRAERPK FEQIVGI 257

Keyword

Alternative splicing; Apoptosis; ATP-binding; Cell membrane; Complete proteome; Developmental protein; Glycoprotein; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0030425	; C:dendrite
GO:0005887	; C:integral to plasma membrane
GO:0031594	; C:neuromuscular junction
GO:0043025	; C:neuronal cell body
GO:0045211	; C:postsynaptic membrane
GO:0005524	; F:ATP binding
GO:0008046	; F:axon guidance receptor activity
GO:0045499	; F:chemorepellent activity
GO:0005004	; F:GPI-linked ephrin receptor activity
GO:0007420	; P:brain development
GO:0048755	; P:branching morphogenesis of a nerve
GO:0043525	; P:positive regulation of neuron apoptotic process
GO:0022407	; P:regulation of cell-cell adhesion
GO:0043281	; P:regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0070372	; P:regulation of ERK1 and ERK2 cascade
GO:0050730	; P:regulation of peptidyl-tyrosine phosphorylation
GO:0031952	; P:regulation of protein autophosphorylation
GO:0031290	; P:retinal ganglion cell axon guidance

KEGG

mmu:13841

;

InterPros

IPR001090	; Ephrin_rcpt_lig-bd_dom.
IPR003961	; Fibronectin_type3.
IPR008979	; Galactose-bd-like.
IPR009030	; Growth_fac_rcpt.
IPR013783	; Ig-like_fold.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR021129	; SAM_type1.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016257	; Tyr_kinase_ephrin_rcpt.
IPR001426	; Tyr_kinase_rcpt_V_CS.

Pfam

PF01404	; Ephrin_lbd; 1.
PF00041	; fn3; 2.
PF07714	; Pkinase_Tyr; 1.
PF00536	; SAM_1; 1.

SMARTs

SM00615	; EPH_lbd; 1.
SM00060	; FN3; 2.
SM00454	; SAM; 1.
SM00219	; TyrKc; 1.

Prosites

PS01186	; EGF_2; UNKNOWN_1.
PS51550	; EPH_LBD; 1.
PS50853	; FN3; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00790	; RECEPTOR_TYR_KIN_V_1; 1.
PS00791	; RECEPTOR_TYR_KIN_V_2; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013