EKS-MUM-00218

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00218

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NKF2	635.7	4.3E-191	126	481	356

Status

Reviewed

Ensembl Protein

ENSMUSP00000101443

UniProt Accession

Q99MQ3; A2AM77; Q7TMZ3; Q811I8; Q91XU5;

Protein Name

Serine/threonine-protein kinase PINK1, mitochondrial

Protein Synonyms/Alias

BRPK; PTEN-induced putative kinase protein 1;

Gene Name

Pink1

Gene Synonyms/Alias

Pink1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000028756	ENSMUSP00000030536	ENSMUST00000030536
ENSMUSG00000028756	ENSMUSP00000101443	ENSMUST00000105817
ENSMUSG00000028756	ENSMUSP00000101442	ENSMUST00000105816

Organism

Mus musculus

Functional Description

Protects against mitochondrial dysfunction duringcellular stress, potentially by phosphorylating mitochondrial proteins (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) (By similarity). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation (By similarity).

Protein Length

550

Protein Sequence
(FASTA)

MAVRQALGRG LQLERPGQVV SPGAQPRPVG LPLPDRYRFF RQSVAGLAAR IQRQFMVRAR 60

GGAGPCGRAV FLAFGLGLGL IEEKQAEGRR AASACQEIQA IFTQKTKRVS DPLDTRCWQG 120

FRLEDYLIGQ AIGKGCNAAV YEATMPTLPQ HLEKAKHLGL IGKGPDVVLK GADGEQAPGT 180

PTFPFAIKMM WNISAGSSSE AILSKMSQEL VPASRVALAG EYGAVTYRRS RDGPKQLAPH 240

PNIIRVFRAF TSSVPLLPGA LADYPDMLPP HYYPEGLGHG RTLFLVMKNY PCTLRQYLEE 300

QTPSSRLATM MTLQLLEGVD HLVQQGIAHR DLKSDNILVE WDSDGCPWLV ISDFGCCLAD 360

QHVGLRLPFN SSSVERGGNG SLMAPEVSTA HSGPSAVIDY SKADTWAVGA IAYEIFGLAN 420

PFYGQGSAHL ESRSYQEAQL PEMPESVPPE ARRLVRSLLQ REASKRPSAR LAANVLHLSL 480

WGEHLLALKN LKLDKMIAWL LQQSAATLLA DRLREKSCVE TKLQMLFLAN LECEALCQAA 540

LLLSSWRAAP 550

Nucleotide Sequence
(FASTA)

ATGGCGGTGC GACAGGCACT GGGCCGAGGC CTGCAGCTGG AGCGCCCAGG CCAGGTCGTA 60

AGCCCCGGAG CACAGCCTCG GCCGGTCGGG CTCCCCCTCC CGGACCGCTA CCGCTTCTTC 120

CGCCAGTCGG TAGCTGGGCT GGCGGCGCGG ATCCAGAGGC AGTTCATGGT GCGGGCCCGG 180

GGCGGCGCAG GCCCTTGCGG CCGAGCGGTC TTCCTGGCCT TCGGGCTGGG GCTGGGGCTG 240

ATCGAGGAGA AGCAGGCGGA AGGCCGGAGG GCCGCCTCGG CCTGTCAGGA GATCCAGGCA 300

ATTTTTACAC AGAAAACCAA GCGCGTGTCT GACCCACTGG ACACTCGATG CTGGCAGGGC 360

TTCCGTCTGG AGGATTATCT GATAGGGCAA GCCATTGGCA AGGGTTGCAA TGCCGCTGTG 420

TATGAAGCCA CCATGCCCAC GCTGCCCCAG CACCTGGAAA AGGCCAAACA CCTTGGCCTT 480

ATAGGAAAGG GCCCGGATGT CGTCCTGAAG GGAGCAGACG GGGAGCAGGC TCCAGGGACT 540

CCCACCTTTC CCTTTGCCAT CAAGATGATG TGGAATATCT CGGCAGGTTC CTCCAGCGAA 600

GCCATCTTAA GCAAAATGAG CCAGGAGCTG GTCCCGGCAA GCCGCGTGGC TTTGGCTGGA 660

GAGTATGGAG CAGTTACTTA CAGAAGATCC AGAGATGGTC CCAAGCAGCT TGCCCCACAC 720

CCTAACATCA TCCGGGTTTT CCGCGCCTTC ACCTCATCTG TGCCCCTCCT GCCGGGGGCC 780

CTGGCTGACT ATCCTGATAT GCTGCCCCCA CACTACTACC CAGAAGGCCT GGGCCACGGT 840

CGCACACTGT TCCTCGTTAT GAAGAACTAC CCCTGTACCC TGCGCCAGTA CCTTGAGGAG 900

CAGACTCCCA GTTCTCGCCT GGCTACCATG ATGACCTTGC AGTTGCTGGA GGGCGTGGAC 960

CATCTGGTTC AGCAGGGCAT TGCCCATCGG GATCTCAAGT CCGACAACAT CCTTGTGGAG 1020

TGGGACTCAG ATGGCTGTCC CTGGCTAGTG ATCTCAGACT TTGGCTGCTG CCTGGCTGAC 1080

CAGCATGTTG GCCTGCGGCT GCCTTTCAAC AGCTCCAGTG TAGAGCGTGG TGGCAATGGC 1140

TCCCTGATGG CCCCTGAGGT GTCCACAGCC CATTCTGGCC CCAGTGCGGT AATTGACTAC 1200

AGCAAAGCCG ATACCTGGGC TGTGGGGGCC ATCGCCTATG AAATCTTTGG GCTTGCCAAT 1260

CCCTTCTATG GCCAAGGCAG TGCCCACCTC GAGAGCCGCA GCTACCAGGA AGCTCAGCTG 1320

CCTGAGATGC CTGAGTCGGT GCCTCCAGAG GCAAGACGGC TGGTGAGGTC ACTGCTCCAG 1380

CGAGAGGCCA GCAAGAGACC GTCTGCACGC TTAGCTGCAA ATGTGCTGCA CTTAAGCCTC 1440

TGGGGCGAGC ATCTTCTAGC CCTGAAGAAC CTGAAATTGG ACAAGATGAT CGCCTGGCTC 1500

TTGCAGCAGT CAGCAGCCAC TCTGCTGGCT GACAGGCTGA GAGAGAAGAG CTGCGTGGAG 1560

ACAAAGCTGC AGATGCTGTT TCTGGCTAAC CTGGAGTGTG AGGCTCTCTG CCAGGCAGCC 1620

CTCCTCCTCT CTTCCTGGAG GGCAGCCCCA TGA 1653

Domain Profile

S: 1     yligealgkgcnaavyeatlatldqelekakhlgllgkevtvvlaeidqesavaakkfpl  60

         ylig+a+gkgcnaavyeat++tl+q+lekakhlgl+gk+++vvl+++d+e+a+++++fp+

Q: 126   YLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHLGLIGKGPDVVLKGADGEQAPGTPTFPF  185

         99**********************************************************

S: 61    aiklmfnieadssseallksmsqelvpasrvaldgeygav........pkllakhPnivr  112

         aik+m+ni+a+sssea+l++msqelvpasrval+geygav        pk+la+hPni+r

Q: 186   AIKMMWNISAGSSSEAILSKMSQELVPASRVALAGEYGAVtyrrsrdgPKQLAPHPNIIR  245

         ************************************************************

S: 113   vlrafidsvkllPdalldyPdalparlypeglgegktlylvmkrydqtlreylesqdrst  172

         v+raf++sv+llP+al+dyPd+lp+++ypeglg+g+tl+lvmk+y++tlr+yle+q++s+

Q: 246   VFRAFTSSVPLLPGALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSS  305

         ************************************************************

S: 173   rlaalllaqlleavdhlvkhgvahrdlksdnilveldddeiPvlviadfGCcladkvdgl  232

         rla+++++qlle+vdhlv++g+ahrdlksdnilve+d+d++P+lvi+dfGCclad+++gl

Q: 306   RLATMMTLQLLEGVDHLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCLADQHVGL  365

         ************************************************************

S: 233   rlpyessevdkGGnaalmaPeistavpGpsavinyekadtWavGalayeifglsnPfyge  292

         rlp++ss+v++GGn++lmaPe+sta++Gpsavi+y+kadtWavGa+ayeifgl+nPfyg+

Q: 366   RLPFNSSSVERGGNGSLMAPEVSTAHSGPSAVIDYSKADTWAVGAIAYEIFGLANPFYGQ  425

         ************************************************************

S: 293   gkaslesasyqeaqlPalpesvppelrqlvydllkreaskrvsadiaanvlqlslf  348

         g+a+les+syqeaqlP++pesvppe+r+lv++ll+reaskr+sa++aanvl+lsl+

Q: 426   GSAHLESRSYQEAQLPEMPESVPPEARRLVRSLLQREASKRPSARLAANVLHLSLW  481

         *******************************************************9

Domain Sequence
(FASTA)

YLIGQAIGKG CNAAVYEATM PTLPQHLEKA KHLGLIGKGP DVVLKGADGE QAPGTPTFPF 60

AIKMMWNISA GSSSEAILSK MSQELVPASR VALAGEYGAV TYRRSRDGPK QLAPHPNIIR 120

VFRAFTSSVP LLPGALADYP DMLPPHYYPE GLGHGRTLFL VMKNYPCTLR QYLEEQTPSS 180

RLATMMTLQL LEGVDHLVQQ GIAHRDLKSD NILVEWDSDG CPWLVISDFG CCLADQHVGL 240

RLPFNSSSVE RGGNGSLMAP EVSTAHSGPS AVIDYSKADT WAVGAIAYEI FGLANPFYGQ 300

GSAHLESRSY QEAQLPEMPE SVPPEARRLV RSLLQREASK RPSARLAANV LHLSLW 356

Keyword

ATP-binding; Autophagy; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Transit peptide; Transmembrane; Transmembrane helix.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0016021	; C:integral to membrane
GO:0005741	; C:mitochondrial outer membrane
GO:0005739	; C:mitochondrion
GO:0005524	; F:ATP binding
GO:0010857	; F:calcium-dependent protein kinase activity
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0097237	; P:cellular response to toxin
GO:0007243	; P:intracellular protein kinase cascade
GO:0000422	; P:mitochondrion degradation
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0033603	; P:positive regulation of dopamine secretion
GO:0043123	; P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0090200	; P:positive regulation of release of cytochrome c from mitochondria
GO:0032226	; P:positive regulation of synaptic transmission, dopaminergic
GO:0043254	; P:regulation of protein complex assembly
GO:0031396	; P:regulation of protein ubiquitination
GO:0006950	; P:response to stress

KEGG

mmu:68943

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013