EKS-MUM-00422

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00422

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	350.7	1.5E-104	651	907	257

Status

Reviewed

Ensembl Protein

ENSMUSP00000030677

UniProt Accession

Q9WTR2; A2AE93; Q0VBN3;

Protein Name

Mitogen-activated protein kinase kinase kinase 6

Protein Synonyms/Alias

Apoptosis signal-regulating kinase 2;

Gene Name

Map3k6

Gene Synonyms/Alias

Map3k6; Ask2, Mapkkk6, Mekk6;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000028862	ENSMUSP00000030677	ENSMUST00000030677

Organism

Mus musculus

Functional Description

Component of a protein kinase signal transductioncascade. Activates the JNK, but not ERK or p38 kinase pathways.

Protein Length

1291

Protein Sequence
(FASTA)

MAGPCPGAGV LERAGSCWQD PLAEALSRGR SSPAVTGRGC ARSRPLSVVY VLTREPGLGV 60

EPGSGTEAEP LPLRCLREAC AQLQGTRPPP QLRSLPFATL ALGDTAALDS FYNADVVVLE 120

VSSSLAQPSL FYHLGVRESF SMTNNVLLCS QAELPDLQAL REDVFQKNSD CVGSYTLIPY 180

VVTATGRVLC GDAGLLRGIA DGLVQAGAGT EALLTPLVGR LVRLLEATPT DSCGYFRETI 240

RQDIRQARER FSGQQLRQEL ARLQRRLDSV ELLSPDIVMN LLLSYRDVQD YSAIIELVET 300

LQALPTCDVA EQHNVCFHYT FALNRRNRPG DREKALAVLL PLVKYEGPVA PDLYCMCGRI 360

YKDMFFTSGF QNAGHLEQAY HWYRKAFDVE PSLHSGINAA VLLIAAGQHF EDSEELRLIG 420

MKLACLLARK GCVEKMQYYW DVGFYLGAQI LANDPIQVVL AAEQLYKLNA PIWYLVSVME 480

TFLLYQHFRP TPEPSGGPPL RAHFWLHFLL QSCQPFKMAP PQEDQCLVLV LEINKVLLPA 540

RLEIQGTDPV STVTLSLLDP ETQEDPSSWT FPVTSICGIS TSKLDQRCCF LYALPPAQDV 600

QLCFPSVERC QRFCGLIQVL VMNPDSSAPT EEAEGAREVL EFDYEYSETG ERLVLGRGTY 660

GVVYAGRDRH TRVRIAIKEI PERDSRFSQP LHEEIALHKR LRHKNIVRYL GSASQGGYLK 720

IFMEEVPGGS LSSLLRSVWG PLKDNESTIS FYTRQILQGL SYLHENRIVH RDIKGDNVLI 780

NTFSGLLKIS DFGTSKRLAG ITPCTETFTG TLQYMAPEII DQGPRGYGKA ADIWSLGCTV 840

IEMATGRPPF HELGSPQAAM FQVGMYKVHP PVPGSLSAEA QAFLLRTFEP DPRLRASAQE 900

LLGDPFLQPG KRSRSPGSPR HTPRPSGAPS GPSSPSADSA TQSQTFPRPQ APSQHPPSPP 960

KRCLSYGDTS QLRVPEEPAA EEPASPEESS GLSLLHQESK RRAMLAAVLE QEVPTLAENL 1020

LEQEQDSRLS KIHVELLLRC LGAQIHTPNR RQLAQELRTL QAQLRAQSLG PALLKGPLFA 1080

FPDAVKQILR RRQIRPHWMF VLDSLLSRAV RAAMAVLDAE SEKKAVLPRS EESSKEESQQ 1140

KPQESQALQS QLPPEQGPPS LMVELGLLRA ETDRLRDLLA EKERECQALV QQALHRVHAE 1200

TRKYAPASET PATLPKDQNL VRWLQELSVD PATIQTLLSH SFTLQTLLTC ATQDDLVYTR 1260

IRGGMVCRIW RAILAQRAGA TSVTPVPRDA E 1291

Nucleotide Sequence
(FASTA)

ATGGCGGGGC CATGTCCTGG GGCCGGGGTT CTAGAACGGG CGGGCAGCTG CTGGCAGGAC 60

CCGCTGGCGG AGGCGCTGAG CCGGGGCCGC TCGTCTCCTG CGGTCACGGG TCGGGGCTGC 120

GCGCGGAGCC GGCCTCTCAG TGTGGTCTAC GTGCTGACCC GGGAGCCGGG GCTCGGGGTG 180

GAGCCCGGGT CGGGAACCGA GGCAGAGCCG CTGCCGTTGC GATGCCTGCG CGAGGCCTGC 240

GCTCAGCTCC AGGGCACACG GCCACCCCCG CAGCTACGCA GCCTACCCTT TGCGACGCTG 300

GCGCTAGGCG ACACCGCTGC GCTGGACTCC TTCTACAACG CGGATGTAGT GGTGCTGGAG 360

GTGAGCAGCT CCCTGGCACA GCCTTCCTTG TTCTACCACC TCGGCGTGCG TGAGAGCTTC 420

AGCATGACCA ACAACGTGCT CCTCTGTTCC CAGGCAGAGC TCCCTGATCT GCAGGCCCTT 480

CGTGAGGATG TTTTCCAGAA GAACTCGGAT TGTGTTGGCA GCTACACACT GATTCCTTAT 540

GTGGTGACAG CCACTGGCCG GGTCTTATGT GGCGATGCAG GCCTCCTGAG GGGCATAGCC 600

GATGGGCTAG TACAGGCTGG GGCGGGCACT GAAGCCCTCC TCACTCCCCT GGTGGGCCGG 660

CTTGTCCGTC TGTTGGAGGC TACACCCACA GACTCTTGCG GCTATTTCCG GGAGACCATT 720

CGTCAGGATA TCCGGCAGGC TCGGGAGCGA TTCAGTGGGC AGCAGCTGAG GCAGGAGCTG 780

GCTCGCCTGC AGCGGAGGCT GGACAGCGTG GAGCTGCTGA GCCCTGACAT TGTCATGAAT 840

CTGCTGCTAT CGTACCGTGA TGTCCAGGAC TACTCGGCCA TCATTGAGCT GGTGGAGACG 900

CTGCAGGCTT TGCCCACCTG TGATGTGGCT GAACAGCACA ATGTCTGCTT TCACTACACA 960

TTTGCCCTCA ACAGGAGGAA CAGGCCTGGG GACCGGGAGA AAGCCCTGGC TGTCCTGCTG 1020

CCATTGGTGA AGTATGAAGG TCCCGTGGCT CCGGATCTGT ACTGCATGTG TGGTCGTATC 1080

TACAAGGACA TGTTCTTCAC TTCTGGCTTC CAAAATGCCG GCCACCTGGA GCAGGCCTAT 1140

CACTGGTACC GCAAGGCGTT TGATGTGGAG CCCAGCCTGC ACTCAGGCAT CAATGCAGCT 1200

GTTCTCCTCA TTGCAGCTGG GCAGCATTTC GAAGACTCTG AGGAGCTCCG GCTGATTGGC 1260

ATGAAGCTGG CCTGCCTGCT AGCCCGCAAA GGCTGTGTGG AGAAGATGCA GTACTACTGG 1320

GATGTGGGAT TCTACCTGGG AGCCCAGATT CTTGCCAACG ACCCCATCCA GGTGGTGCTG 1380

GCTGCAGAGC AACTGTACAA GCTTAATGCC CCCATTTGGT ACTTGGTCTC CGTGATGGAG 1440

ACCTTCCTAC TGTACCAGCA TTTCAGACCC ACACCAGAGC CCTCAGGAGG ACCCCCGCTG 1500

CGAGCTCACT TCTGGCTCCA CTTCCTGCTA CAATCTTGCC AGCCTTTCAA GATGGCTCCT 1560

CCTCAGGAGG ACCAGTGCCT GGTACTGGTG CTGGAGATAA ACAAGGTCCT GCTGCCTGCC 1620

AGGCTTGAGA TTCAGGGAAC AGACCCCGTG AGCACAGTGA CCCTAAGCTT GCTGGACCCA 1680

GAGACTCAGG AGGATCCTTC CAGCTGGACC TTCCCAGTCA CCTCCATCTG TGGGATCAGC 1740

ACCTCCAAGC TGGACCAGCG CTGCTGCTTC CTGTACGCAC TTCCTCCGGC CCAGGACGTC 1800

CAGCTGTGCT TTCCCAGTGT GGAGCGCTGC CAGCGGTTCT GTGGCCTAAT TCAGGTCTTG 1860

GTGATGAATC CGGATTCCTC CGCGCCCACT GAGGAGGCAG AAGGCGCAAG GGAGGTGCTG 1920

GAGTTTGATT ATGAATACTC GGAGACTGGT GAGCGGCTGG TGTTGGGCAG AGGCACGTAT 1980

GGGGTGGTGT ATGCTGGCCG AGACAGGCAC ACGAGGGTAC GAATCGCCAT CAAGGAGATC 2040

CCAGAGAGAG ATAGCAGATT CTCTCAGCCT CTGCATGAAG AGATCGCTCT TCACAAACGA 2100

CTTCGTCATA AGAATATTGT GCGTTATCTG GGCTCAGCCA GCCAGGGCGG CTACCTCAAG 2160

ATCTTCATGG AGGAAGTGCC TGGAGGCAGC CTGTCCTCCT TGCTTAGGTC AGTGTGGGGA 2220

CCCCTAAAGG ACAACGAGAG CACTATTAGC TTCTATACAC GTCAGATCCT ACAAGGACTC 2280

AGCTACCTCC ATGAGAACCG TATCGTTCAC AGAGACATCA AGGGAGACAA TGTTCTGATC 2340

AACACCTTCA GTGGGCTGCT CAAGATTTCT GACTTTGGCA CCTCTAAGCG GCTGGCAGGC 2400

ATCACACCGT GCACGGAGAC TTTCACAGGG ACTCTGCAGT ATATGGCCCC GGAAATCATC 2460

GACCAGGGCC CGCGAGGATA TGGGAAGGCA GCTGACATCT GGTCTCTGGG CTGCACTGTA 2520

ATCGAGATGG CAACAGGTCG ACCGCCCTTC CATGAACTAG GGAGCCCTCA GGCTGCGATG 2580

TTTCAGGTGG GCATGTACAA GGTACATCCA CCCGTGCCCG GTTCCCTGTC AGCTGAGGCC 2640

CAAGCCTTCC TCCTCCGAAC TTTTGAGCCA GATCCCCGCC TCCGAGCCAG TGCCCAAGAG 2700

CTGCTGGGAG ACCCCTTCCT TCAGCCAGGA AAGAGGAGCC GCAGCCCGGG CTCTCCTCGG 2760

CATACTCCCC GGCCCTCAGG CGCCCCTTCC GGTCCTTCCA GTCCTTCAGC TGACTCAGCC 2820

ACACAGTCTC AGACATTCCC AAGGCCCCAG GCACCCTCTC AGCACCCACC CAGCCCCCCA 2880

AAGCGCTGCC TTAGTTATGG GGACACCAGT CAGCTCCGTG TGCCCGAGGA GCCCGCTGCC 2940

GAGGAACCCG CGTCCCCAGA AGAGAGTTCA GGCCTGAGTC TGCTGCACCA GGAGAGCAAG 3000

CGCCGGGCCA TGCTGGCTGC GGTGCTGGAA CAGGAGGTGC CCACACTAGC AGAGAATCTC 3060

CTGGAACAGG AACAGGACTC TCGACTCAGC AAGATTCATG TGGAACTTCT GCTTCGGTGC 3120

CTTGGGGCAC AAATCCACAC TCCTAACCGC CGGCAGCTGG CCCAGGAGCT GCGGACCCTG 3180

CAAGCTCAGC TGCGGGCCCA GAGCCTGGGC CCTGCGCTTT TGAAGGGGCC GCTCTTCGCC 3240

TTTCCAGACG CGGTGAAGCA GATCCTCCGC AGACGCCAGA TCCGCCCACA CTGGATGTTC 3300

GTGTTGGACT CGCTGCTCAG CCGTGCAGTC CGGGCGGCGA TGGCGGTGCT GGACGCGGAG 3360

TCGGAGAAGA AAGCGGTCTT ACCGAGGTCA GAAGAGTCGA GTAAAGAGGA GTCCCAGCAG 3420

AAACCTCAGG AGAGCCAGGC GCTGCAGAGC CAGCTCCCAC CAGAGCAGGG ACCCCCATCG 3480

TTGATGGTGG AGTTGGGCCT TTTGCGAGCC GAGACTGACA GGCTTCGGGA CCTTCTGGCT 3540

GAGAAGGAAC GTGAGTGCCA GGCGCTGGTG CAACAGGCCC TGCATCGGGT GCATGCAGAG 3600

ACCAGGAAGT ATGCCCCAGC TTCAGAGACC CCAGCCACTC TCCCAAAGGA CCAGAACCTG 3660

GTGCGGTGGC TACAGGAACT GAGTGTAGAT CCAGCCACTA TCCAAACGCT CCTGAGTCAT 3720

AGCTTCACCC TTCAAACCCT GCTCACCTGT GCCACTCAAG ATGATCTAGT GTACACCAGA 3780

ATCAGGGGAG GGATGGTATG CCGGATTTGG AGAGCCATCT TGGCACAGCG AGCAGGAGCC 3840

ACATCGGTCA CCCCAGTACC CCGGGACGCT GAGTGA 3876

Domain Profile

S: 3     krevigkGsfgsvyaaldvdtgellavKeveikpekksekkevkalkeEikllkelkHen  62

         +r v+g+G++g vya++d +t+ ++a+Ke+   pe++s+   +++l+eEi+l+k+l+H+n

Q: 651   ERLVLGRGTYGVVYAGRDRHTRVRIAIKEI---PERDSR--FSQPLHEEIALHKRLRHKN  705

         5779**************************...888775..9******************

S: 63    ivkyyGveveeeelkiflEyvegGslsslle.kygkle..esvikkytkqileglayLHe  119

         iv+y+G++++ ++lkif+E v+gGslssll+  +g+l+  es+i++yt+qil+gl+yLHe

Q: 706   IVRYLGSASQGGYLKIFMEEVPGGSLSSLLRsVWGPLKdnESTISFYTRQILQGLSYLHE  765

         *******************************99****9999*******************

S: 120   kkivHRDiKgaNillds.sgvvklsDFGaskrlaelntkaaelesltGtlaymaPEvikk  178

         ++ivHRDiKg+N+l+++ sg++k+sDFG+skrla ++++   +e++tGtl+ymaPE+i++

Q: 766   NRIVHRDIKGDNVLINTfSGLLKISDFGTSKRLAGITPC---TETFTGTLQYMAPEIIDQ  822

         *****************9*********************...8*****************

S: 179   kekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklseegkd  238

         + +gyg++aDiWslgc+viem+tg++Pf+el++++a+mf+vg+ k++P++p +ls+e+++

Q: 823   GPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYKVHPPVPGSLSAEAQA  882

         ************************************************************

S: 239   flekcleldpkkrpsaeeLLkhefl  263

         fl +++e dp+ r+sa+eLL ++fl

Q: 883   FLLRTFEPDPRLRASAQELLGDPFL  907

         **********************996

Domain Sequence
(FASTA)

ERLVLGRGTY GVVYAGRDRH TRVRIAIKEI PERDSRFSQP LHEEIALHKR LRHKNIVRYL 60

GSASQGGYLK IFMEEVPGGS LSSLLRSVWG PLKDNESTIS FYTRQILQGL SYLHENRIVH 120

RDIKGDNVLI NTFSGLLKIS DFGTSKRLAG ITPCTETFTG TLQYMAPEII DQGPRGYGKA 180

ADIWSLGCTV IEMATGRPPF HELGSPQAAM FQVGMYKVHP PVPGSLSAEA QAFLLRTFEP 240

DPRLRASAQE LLGDPFL 257

Keyword

ATP-binding; Coiled coil; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Tursiops truncatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004709	; F:MAP kinase kinase kinase activity

KEGG

mmu:53608

;

InterPros

IPR025136	; DUF4071.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR013761	; SAM/pointed.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF13281	; DUF4071; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013