EKS-MUM-00108

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00108

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Tec	491.3	1.2E-147	347	597	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000073509

UniProt Accession

P24604; Q9R1M9; Q9WVN0; Q9WVN1; Q9WVN2; Q9WVN3;

Protein Name

Tyrosine-protein kinase Tec

Protein Synonyms/Alias

Gene Name

Tec

Gene Synonyms/Alias

Tec;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000029217	ENSMUSP00000073509	ENSMUST00000073843
ENSMUSG00000029217	ENSMUSP00000109224	ENSMUST00000113594
ENSMUSG00000029217	ENSMUSP00000118980	ENSMUST00000155342
ENSMUSG00000029217	ENSMUSP00000071836	ENSMUST00000071944
ENSMUSG00000029217	ENSMUSP00000120155	ENSMUST00000138842
ENSMUSG00000029217	ENSMUSP00000123606	ENSMUST00000126481
ENSMUSG00000029217	ENSMUSP00000123258	ENSMUST00000149533

Organism

Mus musculus

Functional Description

Non-receptor tyrosine kinase that contributes tosignaling from many receptors and participates as a signal transducer in multiple downstream pathways, including regulation of the actin cytoskekleton. Plays a redundant role to ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction. Phosphorylates DOK1, one CD28-specific substrate, and contributes to CD28-signaling. Mediates signals that negatively regulate IL2RA expression induced by TCR cross-linking. Plays a redundant role to BTK in BCR-signaling for B-cell development and activation, especially by phosphorylating STAP1, a BCR-signaling protein. Required in mast cells for efficient cytokine production. Involved in both growth and differentiation mechanisms of myeloid cells through activation by the granulocyte colony-stimulating factor CSF3, a critical cytokine to promoting the growth, differentiation, and functional activation of myeloid cells. Participates in platelet signaling downstream of integrin activation. Cooperates with JAK2 through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. GRB10, a negative modifier of the FOS activation pathway, is another substrate of TEC. TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. Plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-82'. May also be involved in the regulation of osteoclast differentiation.

Protein Length

608

Protein Sequence
(FASTA)

MNFNTILEEI LIKRSQQKKK TSPLNYKERL FVLTKSVLSY YEGRAEKKYR KGVIDISKIK 60

CVEIVKNDDG VIPCQNKFPF QVVHDANTLY IFAPSPQSRD RWVKKLKEEI KNNNNIMIKY 120

HPKFWADGSY QCCRQTEKLA PGCEKYNLFE SSIRKTLPPA PEIKKRRPPP PIPPEEENTE 180

EIVVAMYDFQ ATEAHDLRLE RGQEYIILEK NDLHWWRARD KYGWYCRNTN RSKAEQLLRT 240

EDKEGGFMVR DSSQPGLYTV SLYTKFGGEG SSGFRHYHIK ETATSPKKYY LAEKHAFGSI 300

PEIIEYHKHN AAGLVTRLRY PVSTKGKNAP TTAGFSYDKW EINPSELTFM RELGSGLFGV 360

VRLGKWRAQY KVAIKAIREG AMCEEDFIEE AKVMMKLTHP KLVQLYGVCT QQKPIYIVTE 420

FMERGCLLNF LRQRQGHFSR DMLLSMCQDV CEGMEYLERN SFIHRDLAAR NCLVNEAGVV 480

KVSDFGMARY VLDDQYTSSS GAKFPVKWCP PEVFNYSRFS SKSDVWSFGV LMWEIFTEGR 540

MPFEKNTNYE VVTMVTRGHR LHRPKLASKY LYEVMLRCWQ ERPEGRPSFE DLLRTIDELV 600

ECEETFGR 608

Nucleotide Sequence
(FASTA)

ATGAATTTCA ACACTATCCT AGAAGAGATT CTTATTAAAA GGTCCCAGCA GAAAAAGAAG 60

ACATCACCCT TAAACTACAA AGAGAGACTT TTTGTACTTA CAAAATCCGT GTTGAGCTAC 120

TATGAGGGTC GAGCGGAGAA GAAATACAGA AAGGGCGTCA TTGATATTTC CAAAATCAAG 180

TGTGTGGAGA TAGTGAAGAA CGATGATGGT GTCATTCCCT GTCAAAATAA ATTTCCATTC 240

CAGGTTGTTC ATGATGCTAA TACACTTTAT ATTTTTGCAC CTAGTCCACA AAGCAGGGAC 300

CGATGGGTGA AGAAGTTAAA AGAAGAAATA AAGAACAACA ATAATATCAT GATTAAATAC 360

CATCCTAAAT TCTGGGCAGA TGGGAGTTAC CAGTGTTGTA GACAAACAGA AAAACTAGCA 420

CCCGGATGTG AGAAGTACAA TCTTTTTGAG AGTAGTATAA GAAAGACCCT GCCTCCCGCG 480

CCAGAAATAA AGAAGAGAAG GCCTCCTCCA CCAATTCCCC CAGAGGAAGA AAATACTGAA 540

GAAATCGTTG TAGCGATGTA TGACTTCCAA GCGACGGAAG CACATGACCT CAGGTTAGAG 600

AGAGGCCAAG AGTATATCAT CCTGGAAAAG AATGACCTCC ATTGGTGGAG AGCGAGAGAT 660

AAGTATGGGT GGTACTGCAG AAATACCAAC AGAAGCAAAG CAGAACAGCT CCTCAGAACG 720

GAAGATAAAG AAGGTGGTTT TATGGTGAGA GACTCCAGTC AACCAGGCTT GTACACTGTC 780

TCCCTTTACA CAAAGTTTGG GGGAGAAGGC TCATCAGGTT TCAGGCATTA TCACATAAAG 840

GAAACAGCAA CATCCCCAAA GAAGTATTAC CTGGCAGAGA AGCATGCTTT CGGGTCCATT 900

CCTGAGATCA TTGAATATCA CAAGCACAAT GCGGCAGGGC TTGTCACCAG GCTGCGGTAC 960

CCGGTCAGTA CAAAGGGGAA GAACGCTCCC ACTACTGCCG GCTTCAGCTA TGATAAGTGG 1020

GAGATTAACC CATCAGAGCT GACCTTTATG AGAGAGTTGG GGAGCGGACT GTTTGGAGTG 1080

GTGAGGCTTG GCAAGTGGCG GGCCCAGTAC AAAGTGGCCA TCAAAGCTAT CCGGGAAGGC 1140

GCCATGTGTG AAGAGGATTT CATAGAGGAA GCTAAAGTCA TGATGAAGCT GACACACCCC 1200

AAGCTGGTAC AGCTCTATGG TGTATGCACC CAGCAGAAGC CCATCTACAT CGTTACCGAG 1260

TTCATGGAAC GGGGCTGCCT TCTGAATTTC CTCCGGCAGA GACAAGGCCA TTTCAGCAGA 1320

GACATGCTGC TAAGCATGTG TCAAGATGTC TGTGAAGGGA TGGAGTACCT GGAGAGAAAC 1380

AGCTTCATCC ACAGAGACCT GGCTGCCAGA AATTGTCTAG TGAATGAAGC AGGAGTTGTC 1440

AAAGTATCTG ATTTTGGAAT GGCCAGGTAC GTTCTGGATG ATCAGTACAC AAGTTCTTCT 1500

GGCGCCAAGT TCCCTGTGAA GTGGTGTCCC CCAGAAGTGT TTAATTACAG CCGCTTTAGC 1560

AGCAAGTCAG ACGTCTGGTC GTTTGGTGTG CTAATGTGGG AAATATTCAC AGAAGGCAGG 1620

ATGCCCTTTG AGAAGAACAC CAATTACGAA GTGGTAACCA TGGTGACTCG TGGCCACCGC 1680

CTCCACCGGC CAAAGCTGGC TTCCAAATAT TTGTATGAGG TGATGCTGAG ATGCTGGCAA 1740

GAGAGACCAG AGGGAAGGCC TTCCTTTGAA GACTTGCTGC GTACGATAGA TGAACTAGTT 1800

GAATGTGAAG AAACTTTTGG AAGATGA 1827

Domain Profile

S: 1     ltllkelgsgqfgvvklgkwrakidvaikaikegamseedfieeakvmmklshpklvqly  60

         lt+++elgsg fgvv+lgkwra+++vaikai+egam+eedfieeakvmmkl+hpklvqly

Q: 347   LTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLY  406

         79**********************************************************

S: 61    gvclkqkpiyivteylengcllnylrekkkklekelllemckdvcegmeylerksfihrd  120

         gvc++qkpiyivte++e+gclln+lr++++++++++ll+mc+dvcegmeyler+sfihrd

Q: 407   GVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDMLLSMCQDVCEGMEYLERNSFIHRD  466

         ************************************************************

S: 121   laarnclvdeelvvkvsdfglaryvlddqyvssvgakfpvkwsppevlsyskyssksdvw  180

         laarnclv+e+ vvkvsdfg+aryvlddqy+ss+gakfpvkw+ppev++ys++ssksdvw

Q: 467   LAARNCLVNEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVW  526

         ************************************************************

S: 181   afgvlmwevfsegklpyekksnsevvekvsrglrlyrpklaskkvyevmkscwkekpeer  240

         +fgvlmwe+f+eg++p+ek++n+evv++v+rg+rl+rpklask++yevm +cw+e+pe+r

Q: 527   SFGVLMWEIFTEGRMPFEKNTNYEVVTMVTRGHRLHRPKLASKYLYEVMLRCWQERPEGR  586

         ************************************************************

S: 241   ptfkellsale  251

         p+f++ll+++

Q: 587   PSFEDLLRTID  597

         ********995

Domain Sequence
(FASTA)

LTFMRELGSG LFGVVRLGKW RAQYKVAIKA IREGAMCEED FIEEAKVMMK LTHPKLVQLY 60

GVCTQQKPIY IVTEFMERGC LLNFLRQRQG HFSRDMLLSM CQDVCEGMEY LERNSFIHRD 120

LAARNCLVNE AGVVKVSDFG MARYVLDDQY TSSSGAKFPV KWCPPEVFNY SRFSSKSDVW 180

SFGVLMWEIF TEGRMPFEKN TNYEVVTMVT RGHRLHRPKL ASKYLYEVML RCWQERPEGR 240

PSFEDLLRTI D 251

Keyword

3D-structure; Adaptive immunity; Alternative splicing; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Immunity; Kinase; Lipid-binding; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Equus caballus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005856	; C:cytoskeleton
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0005543	; F:phospholipid binding
GO:0035556	; P:intracellular signal transduction

KEGG

mmu:21682

;

InterPros

IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR001849	; Pleckstrin_homology.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR001452	; SH3_domain.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR001562	; Znf_Btk_motif.

Pfam

PF00779	; BTK; 1.
PF00169	; PH; 1.
PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00107	; BTK; 1.
SM00233	; PH; 1.
SM00252	; SH2; 1.
SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS50002	; SH3; 1.
PS51113	; ZF_BTK; 1.

Prints

PR00401	; SH2DOMAIN.
PR00452	; SH3DOMAIN.
PR00402	; TECBTKDOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013