EKS-MUM-00433

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00433

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/PDGFR	615.1	9.8E-185	593	950	358

Status

Reviewed

Ensembl Protein

ENSMUSP00000000476

UniProt Accession

P26618; Q3TQ37; Q62046; Q7TSJ3; Q8C4N3;

Protein Name

Platelet-derived growth factor receptor alpha

Protein Synonyms/Alias

PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; Platelet-derived growth factor alpha receptor;

Gene Name

Pdgfra

Gene Synonyms/Alias

Pdgfra;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000029231	ENSMUSP00000127173	ENSMUST00000168162
ENSMUSG00000029231	ENSMUSP00000000476	ENSMUST00000000476

Organism

Mus musculus

Functional Description

Tyrosine-protein kinase that acts as a cell-surfacereceptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.

Protein Length

1089

Protein Sequence
(FASTA)

MGTSHQVFLV LSCLLTGPGL ISCQLLLPSI LPNENEKIVQ LNSSFSLRCV GESEVSWQHP 60

MSEEDDPNVE IRSEENNSGL FVTVLEVVNA SAAHTGWYTC YYNHTQTDES EIEGRHIYIY 120

VPDPDMAFVP LGMTDSLVIV EEDDSAIIPC RTTDPETQVT LHNNGRLVPA SYDSRQGFNG 180

TFSVGPYICE ATVKGRTFKT SEFNVYALKA TSELNLEMDA RQTVYKAGET IVVTCAVFNN 240

EVVDLQWTYP GEVRNKGITM LEEIKLPSIK LVYTLTVPKA TVKDSGEYEC AARQATKEVK 300

EMKRVTISVH EKGFVEIEPT FGQLEAVNLH EVREFVVEVQ AYPTPRISWL KDNLTLIENL 360

TEITTDVQKS QETRYQSKLK LIRAKEEDSG HYTIIVQNED DVKSYTFELS TLVPASILDL 420

VDDHHGSGGG QTVRCTAEGT PLPEIDWMIC KHIKKCNNDT SWTVLASNVS NIITELPRRG 480

RSTVEGRVSF AKVEETIAVR CLAKNNLSVV ARELKLVAPT LRSELTVAAA VLVLLVIVIV 540

SLIVLVVIWK QKPRYEIRWR VIESISPDGH EYIYVDPMQL PYDSRWEFPR DGLVLGRILG 600

SGAFGKVVEG TAYGLSRSQP VMKVAVKMLK PTARSSEKQA LMSELKIMTH LGPHLNIVNL 660

LGACTKSGPI YIITEYCFYG DLVNYLHKNR DSFMSQHPEK PKKDLDIFGL NPADESTRSY 720

VILSFENNGD YMDMKQADTT QYVPMLERKE VSKYSDIQRS LYDRPASYKK KSMLDSEVKN 780

LLSDDDSEGL TLLDLLSFTY QVARGMEFLA SKNCVHRDLA ARNVLLAQGK IVKICDFGLA 840

RDIMHDSNYV SKGSTFLPVK WMAPESIFDN LYTTLSDVWS YGILLWEIFS LGGTPYPGMM 900

VDSTFYNKIK SGYRMAKPDH ATSEVYEIMV QCWNSEPEKR PSFYHLSEIV ENLLPGQYKK 960

SYEKIHLDFL KSDHPAVARM RVDSDNAYIG VTYKNEEDKL KDWEGGLDEQ RLSADSGYII 1020

PLPDIDPVPE EEDLGKRNRH SSQTSEESAI ETGSSSSTFI KREDETIEDI DMMDDIGIDS 1080

SDLVEDSFL 1089

Nucleotide Sequence
(FASTA)

ATGGGGACCT CCCACCAGGT CTTTCTGGTC CTCAGCTGTC TCCTCACAGG GCCGGGCCTC 60

ATCTCCTGCC AGCTCTTATT ACCCTCTATC CTCCCAAACG AGAATGAGAA GATTGTGCAG 120

CTGAATTCGT CCTTTTCTCT GAGATGCGTT GGGGAGAGTG AAGTGAGCTG GCAGCACCCC 180

ATGTCTGAAG AAGACGACCC CAACGTGGAA ATCAGAAGTG AGGAGAACAA CAGTGGCCTC 240

TTTGTCACAG TGCTGGAAGT GGTTAACGCC TCCGCAGCCC ACACCGGATG GTACACTTGC 300

TACTACAACC ACACTCAGAC GGATGAGAGT GAGATCGAAG GCAGGCACAT TTACATCTAT 360

GTACCAGACC CAGACATGGC CTTTGTGCCT CTCGGGATGA CCGATTCCTT AGTCATCGTG 420

GAAGAGGATG ACTCTGCCAT CATACCTTGC CGCACCACAG ATCCGGAGAC TCAAGTAACC 480

TTGCACAATA ACGGGAGGCT GGTGCCTGCC TCCTATGACA GCAGGCAGGG CTTCAACGGA 540

ACCTTCAGCG TGGGGCCTTA CATCTGTGAG GCCACCGTCA AAGGGAGGAC GTTCAAGACC 600

AGCGAGTTTA ATGTTTATGC CTTGAAAGCA ACGTCAGAAC TGAATCTGGA GATGGACGCA 660

CGCCAGACTG TGTATAAGGC AGGAGAAACG ATCGTGGTGA CCTGTGCCGT CTTTAACAAC 720

GAGGTCGTTG ACCTGCAGTG GACTTACCCT GGAGAAGTGA GAAACAAAGG TATCACCATG 780

CTGGAGGAGA TCAAACTCCC ATCCATCAAA CTGGTGTACA CTTTGACCGT CCCCAAGGCC 840

ACGGTGAAGG ACAGTGGAGA GTATGAATGT GCTGCCCGTC AGGCCACTAA AGAGGTCAAG 900

GAAATGAAGA GAGTCACCAT TTCTGTCCAC GAGAAAGGCT TCGTCGAGAT TGAGCCCACC 960

TTTGGCCAGC TGGAAGCTGT CAACTTGCAC GAAGTCAGAG AGTTCGTGGT GGAGGTGCAG 1020

GCCTACCCGA CGCCCAGGAT ATCGTGGCTG AAGGACAACT TGACTCTGAT AGAGAATCTC 1080

ACCGAGATCA CCACTGATGT ACAGAAGAGT CAGGAAACAA GGTATCAGAG CAAATTAAAG 1140

CTGATCCGGG CTAAGGAAGA AGACAGTGGC CATTACACCA TTATAGTTCA AAATGAGGAT 1200

GACGTGAAGA GTTACACGTT TGAGCTGTCA ACCCTAGTTC CTGCATCCAT TTTGGATCTC 1260

GTAGATGACC ACCATGGCTC TGGCGGGGGA CAGACTGTGA GGTGTACAGC CGAAGGCACC 1320

CCTCTTCCAG AAATCGACTG GATGATCTGC AAGCATATTA AGAAATGTAA TAATGACACT 1380

TCGTGGACAG TTTTGGCCAG CAATGTCTCA AATATTATCA CAGAGCTCCC TCGCCGTGGC 1440

AGGAGTACCG TGGAGGGACG AGTGTCCTTC GCCAAAGTGG AAGAGACCAT CGCAGTTCGA 1500

TGCCTGGCAA AGAACAACCT CAGCGTTGTG GCCCGTGAGC TGAAGCTGGT GGCTCCCACT 1560

CTGCGATCTG AACTCACAGT GGCGGCCGCA GTGTTGGTGC TGTTGGTGAT TGTCATTGTC 1620

TCTCTCATCG TCCTGGTGGT CATTTGGAAG CAGAAACCAC GGTATGAAAT TCGCTGGAGG 1680

GTTATCGAGT CAATCAGCCC CGATGGACAT GAGTATATCT ATGTGGACCC CATGCAGTTG 1740

CCTTACGACT CCAGATGGGA ATTCCCGAGA GATGGCCTCG TGCTTGGTCG GATTTTGGGA 1800

TCCGGTGCAT TTGGGAAAGT GGTCGAAGGT ACAGCTTATG GATTAAGCCG GTCCCAACCT 1860

GTAATGAAGG TGGCTGTGAA GATGCTCAAA CCCACAGCCA GGTCTAGTGA AAAGCAGGCT 1920

CTCATGTCTG AGCTGAAGAT AATGACTCAC CTGGGGCCAC ATTTGAACAT TGTGAACTTG 1980

CTGGGAGCCT GCACCAAGTC AGGTCCCATT TACATCATCA CAGAATACTG CTTCTATGGG 2040

GATTTGGTCA ACTACTTGCA TAAGAACAGA GATAGCTTCA TGAGCCAACA CCCAGAGAAG 2100

CCGAAGAAAG ACCTGGACAT CTTTGGATTG AATCCTGCAG ACGAGAGCAC AAGAAGTTAT 2160

GTGATTTTGT CTTTTGAAAA TAACGGCGAC TACATGGACA TGAAGCAAGC TGATACCACA 2220

CAGTATGTCC CCATGCTTGA AAGGAAAGAG GTTTCTAAAT ACTCTGACAT CCAGAGATCG 2280

CTGTACGATC GGCCAGCCTC CTACAAGAAG AAATCCATGC TAGACTCAGA AGTCAAAAAC 2340

CTCCTTTCGG ACGATGACTC CGAGGGTCTG ACTTTGCTGG ATCTATTGAG CTTCACCTAT 2400

CAAGTTGCTC GAGGAATGGA ATTTTTGGCT TCGAAAAATT GTGTCCACCG GGACCTGGCT 2460

GCCCGCAACG TCCTCCTGGC GCAAGGAAAA ATTGTGAAGA TCTGTGATTT TGGCCTGGCC 2520

AGAGACATCA TGCACGATTC CAACTACGTG TCAAAAGGCA GTACTTTCCT GCCTGTGAAG 2580

TGGATGGCAC CCGAGAGCAT CTTCGACAAC CTCTACACCA CGCTGAGTGA CGTCTGGTCC 2640

TATGGCATTC TGCTCTGGGA AATCTTTTCC CTTGGTGGCA CACCCTATCC TGGCATGATG 2700

GTCGATTCTA CTTTCTACAA TAAGATCAAG AGTGGATACC GGATGGCCAA ACCTGACCAT 2760

GCCACCAGTG AAGTCTATGA GATCATGGTG CAGTGCTGGA ACAGTGAGCC CGAGAAGAGA 2820

CCCTCCTTCT ACCACCTCAG CGAGATAGTG GAGAACCTGT TGCCGGGACA ATACAAGAAG 2880

AGTTATGAAA AGATTCACCT GGACTTCCTA AAGAGTGACC ATCCGGCCGT GGCACGCATG 2940

CGGGTGGACT CTGATAATGC GTACATCGGT GTCACTTACA AAAATGAAGA GGATAAGCTG 3000

AAGGACTGGG AAGGTGGCCT GGACGAACAG AGACTGAGCG CTGACAGTGG CTACATCATC 3060

CCCCTGCCAG ACATTGACCC TGTTCCAGAG GAGGAAGACC TGGGCAAGAG GAACAGACAC 3120

AGCTCACAGA CTTCGGAAGA GAGTGCCATC GAGACAGGTT CCAGTAGTTC CACCTTCATC 3180

AAGAGAGAGG ACGAGACCAT CGAGGACATC GACATGATGG ATGACATTGG TATAGATTCC 3240

TCGGACCTGG TGGAGGACAG CTTCCTGTAA 3270

Domain Profile

S: 1     lelGkvlGsGafGkvveatayGlsksdavlkvavkmlkstarssekealmselkilshlG  60

         l+lG++lGsGafGkvve+tayGls+s++v+kvavkmlk+tarssek+almselki++hlG

Q: 593   LVLGRILGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLG  652

         78**********************************************************

S: 61    ehenivnllGactkgGpiyviteyccyGdllnylrrkresflkkslekkkekeeeldska  120

         +h+nivnllGactk+Gpiy+iteyc+yGdl+nyl+++r+sf++++ ek   ++++ld+++

Q: 653   PHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFMSQHPEK---PKKDLDIFG  709

         *********************************************999...999******

S: 121   l.ladkslrsksslssesseeyvdlkkd................vsdvssskadklasek  163

         l +ad+s+rs+++ls+e++++y+d+k++                +sd+++s +d++as+k

Q: 710   LnPADESTRSYVILSFENNGDYMDMKQAdttqyvpmlerkevskYSDIQRSLYDRPASYK  769

         *9*************************9********************************

S: 164   kksalerevkaaleeedsevleledllsfsyqvakGmeflaskncvhrdlaarnvllteG  223

         kks+l++evk+ l+++dse+l+l dllsf+yqva+Gmeflaskncvhrdlaarnvll++G

Q: 770   KKSMLDSEVKNLLSDDDSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQG  829

         ************************************************************

S: 224   kvvkicdfGlardimkdsnyvvkGnarlpvkwmapesifdslytvksdvwsyGillweif  283

         k+vkicdfGlardim+dsnyv+kG+++lpvkwmapesifd+lyt+ sdvwsyGillweif

Q: 830   KIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIF  889

         ************************************************************

S: 284   slGstpypgllvdskfyklikeGyrmaqpayateeiyeimqkcweaepekrpsfkqlvel  343

         slG+tpypg++vds+fy++ik+Gyrma+p++at+e+yeim++cw++epekrpsf++l+e+

Q: 890   SLGGTPYPGMMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVQCWNSEPEKRPSFYHLSEI  949

         *********************************************************998

S: 344   l  344

Q: 950   V  950

Domain Sequence
(FASTA)

LVLGRILGSG AFGKVVEGTA YGLSRSQPVM KVAVKMLKPT ARSSEKQALM SELKIMTHLG 60

PHLNIVNLLG ACTKSGPIYI ITEYCFYGDL VNYLHKNRDS FMSQHPEKPK KDLDIFGLNP 120

ADESTRSYVI LSFENNGDYM DMKQADTTQY VPMLERKEVS KYSDIQRSLY DRPASYKKKS 180

MLDSEVKNLL SDDDSEGLTL LDLLSFTYQV ARGMEFLASK NCVHRDLAAR NVLLAQGKIV 240

KICDFGLARD IMHDSNYVSK GSTFLPVKWM APESIFDNLY TTLSDVWSYG ILLWEIFSLG 300

GTPYPGMMVD STFYNKIKSG YRMAKPDHAT SEVYEIMVQC WNSEPEKRPS FYHLSEIV 358

Keyword

Alternative splicing; ATP-binding; Cell membrane; Chemotaxis; Complete proteome; Developmental protein; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Proto-oncogene; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0009897	; C:external side of plasma membrane
GO:0005887	; C:integral to plasma membrane
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0005018	; F:platelet-derived growth factor alpha-receptor activity
GO:0048407	; F:platelet-derived growth factor binding
GO:0042803	; F:protein homodimerization activity
GO:0005021	; F:vascular endothelial growth factor-activated receptor activity
GO:0030325	; P:adrenal gland development
GO:0055003	; P:cardiac myofibril assembly
GO:0060326	; P:cell chemotaxis
GO:0071230	; P:cellular response to amino acid stimulus
GO:0007204	; P:elevation of cytosolic calcium ion concentration
GO:0048701	; P:embryonic cranial skeleton morphogenesis
GO:0048557	; P:embryonic digestive tract morphogenesis
GO:0008210	; P:estrogen metabolic process
GO:0030198	; P:extracellular matrix organization
GO:0060325	; P:face morphogenesis
GO:0042063	; P:gliogenesis
GO:0001701	; P:in utero embryonic development
GO:0048839	; P:inner ear development
GO:0033327	; P:Leydig cell differentiation
GO:0030324	; P:lung development
GO:0001553	; P:luteinization
GO:0030539	; P:male genitalia development
GO:0072277	; P:metanephric glomerular capillary formation
GO:0010544	; P:negative regulation of platelet activation
GO:0042475	; P:odontogenesis of dentin-containing tooth
GO:0060021	; P:palate development
GO:0018108	; P:peptidyl-tyrosine phosphorylation
GO:0048015	; P:phosphatidylinositol-mediated signaling
GO:0070527	; P:platelet aggregation
GO:0030335	; P:positive regulation of cell migration
GO:0038091	; P:positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway
GO:0045740	; P:positive regulation of DNA replication
GO:0070374	; P:positive regulation of ERK1 and ERK2 cascade
GO:0048146	; P:positive regulation of fibroblast proliferation
GO:0043552	; P:positive regulation of phosphatidylinositol 3-kinase activity
GO:0010863	; P:positive regulation of phospholipase C activity
GO:0046777	; P:protein autophosphorylation
GO:0050920	; P:regulation of chemotaxis
GO:2000739	; P:regulation of mesenchymal stem cell differentiation
GO:0034097	; P:response to cytokine stimulus
GO:0032355	; P:response to estradiol stimulus
GO:0055093	; P:response to hyperoxia
GO:0010035	; P:response to inorganic substance
GO:0061298	; P:retina vasculature development in camera-type eye
GO:0023019	; P:signal transduction involved in regulation of gene expression
GO:0048010	; P:vascular endothelial growth factor receptor signaling pathway

KEGG

mmu:18595

;

InterPros

IPR007110	; Ig-like.
IPR013783	; Ig-like_fold.
IPR013098	; Ig_I-set.
IPR003599	; Ig_sub.
IPR003598	; Ig_sub2.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016243	; Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824	; Tyr_kinase_rcpt_3_CS.
IPR009134	; Tyr_kinase_VEGFR_rcpt_N.

Pfam

PF07679	; I-set; 2.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00409	; IG; 2.
SM00408	; IGc2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50835	; IG_LIKE; 3.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS00240	; RECEPTOR_TYR_KIN_III; 1.

Prints

PR01832

; VEGFRECEPTOR.

Created Date

20-Feb-2013