EKS-MUM-00532

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00532

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/BRD	N/A	N/A	N/A	N/A	N/A

Status

Reviewed

Ensembl Protein

ENSMUSP00000031215

UniProt Accession

Q91Y44; G3X8Z8; Q59HJ4;

Protein Name

Bromodomain testis-specific protein

Protein Synonyms/Alias

Bromodomain-containing female sterile homeotic-like protein; RING3-like protein;

Gene Name

Brdt

Gene Synonyms/Alias

Brdt; Fsrg3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000029279	ENSMUSP00000108297	ENSMUST00000112677
ENSMUSG00000029279	ENSMUSP00000031215	ENSMUST00000031215

Organism

Mus musculus

Functional Description

Testis-specific chromatin protein that specificallybinds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.

Protein Length

956

Protein Sequence
(FASTA)

MSLPSRQTAI VNPPPPEYIN TKKSGRLTNQ LQFLQRVVLK ALWKHGFSWP FQQPVDAVKL 60

KLPDYYTIIK TPMDLNTIKK RLENKYYEKA SECIEDFNTM FSNCYLYNKT GDDIVVMAQA 120

LEKLFMQKLS QMPQEEQVVG GKERIKKDIQ QKIAVSSAKE QIPSKAAENV FKRQEIPSGL 180

PDISLSPLNM AQEAPPICDS QSLVQITKGV KRRADTTTPT TSIAKASSES PPTLRETKPV 240

NMPVKENTVK NVLPDSQQQH KVLKTVKVTE QLKHCSEILK EMLAKKHLPY AWPFYNPVDA 300

DALGLHNYYD VVKNPMDLGT IKGKMDNQEY KDAYEFAADV RLMFMNCYKY NPPDHEVVAM 360

ARTLQDVFEL HFAKIPDEPI ESMHACHLTT NSAQALSRES SSEASSGDAS SEDSEDERVQ 420

HLAKLQEQLN AVHQQLQVLS QVPLRKLKKK NEKSKRAPKR KKVNNRDENP RKKPKQMKQK 480

EKAKINQPKK KKPLLKSEEE DNAKPMNYDE KRQLSLDINK LPGDKLGRIV HIIQSREPSL 540

RNSNPDEIEI DFETLKASTL RELEKYVLAC LRKRSLKPQA KKVVRSKEEL HSEKKLELER 600

RLLDVNNQLN CRKRQTKRPA KVEKPPPPPP PPPPPPPPPE LASGSRLTDS SSSSGSGSGS 660

SSSSSGSSSS SSSSGSASSS SDSSSSDSSD SEPEIFPKFT GVKQNDLPPK ENIKQIQSSV 720

QDITSAEAPL AQQSTAPCGA PGKHSQQMLG CQVTQHLQAT ENTASVQTQP LSGDCKRVLL 780

GPPVVHTSAE SLTVLEPECH APAQKDIKIK NADSWKSLGK PVKASSVLKS SDELFNQFRK 840

AAIEKEVKAR TQEQMRKHLE HNAKDPKVSQ ENQREPGSGL TLESLSSKVQ DKSLEEDQSE 900

QQPPSEAQDV SKLWLLKDRN LAREKEQERR RREAMAGTID MTLQSDIMTM FENNFD 956

Nucleotide Sequence
(FASTA)

ATGTCTCTGC CAAGTCGACA AACAGCTATT GTTAACCCCC CTCCACCAGA GTACATAAAC 60

ACTAAGAAGA GTGGGCGGTT GACGAATCAG CTGCAGTTCC TACAGAGGGT TGTGCTGAAG 120

GCCCTGTGGA AGCACGGCTT CTCTTGGCCT TTCCAACAGC CGGTGGACGC CGTGAAACTA 180

AAGCTGCCTG ACTATTACAC CATCATAAAA ACCCCAATGG ATTTAAATAC AATTAAGAAG 240

CGGTTGGAAA ATAAGTATTA TGAGAAGGCT TCCGAATGCA TAGAAGACTT CAACACAATG 300

TTCTCAAACT GCTACTTATA CAACAAGACT GGAGATGACA TTGTTGTTAT GGCCCAAGCT 360

CTAGAGAAGT TATTTATGCA GAAATTATCT CAGATGCCAC AAGAAGAGCA AGTTGTGGGT 420

GGTAAGGAGA GAATAAAGAA AGATATCCAA CAAAAGATAG CGGTTTCCTC TGCTAAGGAA 480

CAAATCCCCT CCAAAGCGGC AGAGAATGTC TTTAAGCGGC AAGAGATTCC TTCTGGGCTT 540

CCTGACATCT CTCTCTCGCC CTTAAACATG GCACAAGAAG CTCCCCCGAT CTGTGACTCG 600

CAGTCTCTGG TCCAAATTAC AAAGGGTGTG AAGAGGCGAG CAGACACAAC AACTCCTACC 660

ACTTCCATAG CTAAAGCAAG TAGCGAATCT CCTCCGACAC TTAGAGAAAC AAAGCCAGTG 720

AACATGCCAG TAAAAGAAAA TACAGTAAAG AACGTTTTGC CAGACTCTCA GCAACAACAC 780

AAAGTTTTAA AAACAGTCAA AGTAACGGAG CAATTAAAGC ACTGCAGCGA GATTCTTAAA 840

GAAATGCTTG CCAAGAAACA TTTGCCGTAT GCATGGCCCT TCTATAATCC TGTGGATGCT 900

GATGCTTTGG GACTCCACAA CTACTATGAC GTTGTCAAAA ATCCCATGGA TCTCGGAACG 960

ATCAAGGGGA AAATGGACAA TCAAGAATAT AAGGATGCAT ATGAATTTGC TGCAGATGTC 1020

AGACTGATGT TCATGAACTG TTACAAGTAT AATCCTCCCG ATCATGAAGT TGTGGCGATG 1080

GCTCGGACCC TTCAGGATGT TTTTGAACTA CATTTTGCCA AGATTCCCGA TGAACCTATT 1140

GAGAGTATGC ATGCATGCCA CCTTACAACA AACAGTGCAC AAGCCCTCAG CAGAGAAAGC 1200

AGCAGCGAGG CCTCCTCGGG GGACGCCTCT TCTGAAGACT CTGAGGATGA ACGAGTGCAG 1260

CATCTTGCCA AGCTTCAGGA GCAGCTTAAT GCTGTTCATC AGCAGCTGCA AGTTCTGTCC 1320

CAAGTTCCAT TACGTAAGCT GAAGAAAAAG AATGAGAAGT CCAAAAGGGC GCCAAAAAGG 1380

AAAAAGGTTA ATAACAGAGA TGAAAACCCA AGGAAAAAGC CTAAGCAAAT GAAGCAAAAG 1440

GAGAAGGCCA AAATCAATCA ACCAAAGAAG AAGAAGCCGC TTTTGAAATC GGAAGAGGAA 1500

GACAATGCGA AGCCTATGAA CTACGATGAG AAAAGGCAGC TGAGCTTGGA CATAAACAAA 1560

CTGCCTGGAG ACAAGCTTGG TCGAATAGTT CACATAATAC AGTCAAGGGA GCCTTCCCTG 1620

AGAAACTCCA ACCCGGACGA GATCGAGATC GACTTTGAGA CCCTGAAGGC ATCCACACTT 1680

AGAGAACTGG AGAAGTATGT CCTGGCCTGT CTGAGGAAGA GGTCGCTGAA GCCCCAAGCA 1740

AAGAAGGTTG TCAGGTCTAA GGAGGAGCTC CATTCTGAAA AAAAGCTGGA GCTGGAGAGG 1800

CGGTTGCTGG ACGTCAACAA TCAGCTGAAT TGTAGGAAAC GGCAAACGAA ACGTCCAGCA 1860

AAAGTGGAGA AGCCGCCCCC GCCCCCGCCC CCGCCCCCGC CCCCGCCGCC TCCCCCGGAG 1920

CTGGCCAGTG GGTCTCGCCT GACCGACAGC AGCAGCAGCA GCGGCAGCGG CAGCGGCAGC 1980

AGCAGCAGCA GCAGCGGCAG CAGCAGCAGC AGCAGCTCCT CGGGGTCCGC GAGCAGCAGC 2040

AGCGACTCGA GCTCCTCCGA CAGCAGCGAT TCTGAACCAG AAATATTTCC TAAATTTACT 2100

GGAGTAAAAC AGAATGATTT ACCTCCAAAA GAAAATATAA AGCAGATACA ATCTTCTGTG 2160

CAAGACATAA CCTCTGCTGA AGCTCCCCTT GCTCAGCAGA GCACAGCACC ATGCGGAGCC 2220

CCAGGGAAGC ACTCCCAGCA GATGCTTGGT TGCCAAGTGA CACAGCATCT GCAGGCCACA 2280

GAGAACACTG CATCTGTGCA GACTCAGCCT CTTTCAGGTG ATTGCAAGCG AGTATTGCTT 2340

GGCCCCCCTG TGGTGCACAC CTCTGCTGAG AGCCTCACAG TGCTGGAGCC AGAATGCCAT 2400

GCTCCCGCGC AGAAGGATAT AAAGATTAAG AATGCAGACT CTTGGAAAAG TTTAGGGAAG 2460

CCAGTGAAAG CATCAAGTGT ACTGAAGTCT TCAGATGAGC TTTTCAACCA GTTTAGAAAA 2520

GCTGCAATAG AAAAGGAGGT AAAGGCCCGG ACACAGGAGC AGATGCGGAA ACACCTGGAG 2580

CATAATGCGA AGGACCCAAA AGTATCTCAA GAAAATCAGA GGGAGCCTGG AAGTGGATTG 2640

ACTCTAGAAT CTTTATCAAG TAAAGTGCAA GATAAGTCTC TTGAAGAAGA TCAGAGTGAA 2700

CAGCAGCCAC CGTCGGAAGC TCAAGACGTA AGCAAGCTCT GGCTTCTCAA AGACCGGAAT 2760

CTAGCGAGGG AGAAAGAGCA AGAGCGCAGG AGAAGAGAAG CAATGGCGGG TACCATTGAT 2820

ATGACTCTTC AAAGTGACAT TATGACAATG TTTGAAAATA ACTTTGATTA A 2871

Domain Profile

N/A

Domain Sequence
(FASTA)

N/A

Keyword

3D-structure; Activator; Alternative splicing; Bromodomain; Chromatin regulator; Coiled coil; Complete proteome; Differentiation; Meiosis; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spermatogenesis; Transcription; Transcription regulation.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Takifugu rubripes"; ?>Xenopus tropicalis"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0070577	; F:histone acetyl-lysine binding
GO:0030154	; P:cell differentiation
GO:0006338	; P:chromatin remodeling
GO:0001207	; P:histone displacement
GO:0007141	; P:male meiosis I
GO:0006397	; P:mRNA processing
GO:0051039	; P:positive regulation of transcription during meiosis
GO:0043484	; P:regulation of RNA splicing
GO:0008380	; P:RNA splicing
GO:0007283	; P:spermatogenesis
GO:0006351	; P:transcription, DNA-dependent

KEGG

mmu:114642

;

InterPros

IPR001487	; Bromodomain.
IPR018359	; Bromodomain_CS.

Pfam

PF00439

; Bromodomain; 2.

SMARTs

SM00297

; BROMO; 2.

Prosites

PS00633	; BROMODOMAIN_1; 2.
PS50014	; BROMODOMAIN_2; 2.
PS51525	; NET; 1.

Prints

PR00503

; BROMODOMAIN.

Created Date

20-Feb-2013