| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00239 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CAMK/MAPKAPK | 417.8 | 4.0E-125 | 23 | 304 | 282 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000119182 |
| UniProt Accession | O54992; E9QQ45; Q6QME4; Q6QME5; Q6QME6; Q6QME7; |
| Protein Name | MAP kinase-activated protein kinase 5 |
| Protein Synonyms/Alias | MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; |
| Gene Name | Mapkapk5 |
| Gene Synonyms/Alias | Mapkapk5; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Tumor suppressor serine/threonine-protein kinaseinvolved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. |
| Protein Length | 369 |
Protein Sequence
(FASTA) | | MSEDSDMEKA IKETSILEEY SINWTQKLGA GISGPVRVCV KKSTQERFAL KILLDRPKAR 60 | | NEVRLHMMCA THPNIVQIIE VFANSVQFPH ESSPRARLLI VMEMMEGGEL FHRISQHRHF 120 | | TEKQASQVTK QIALALQHCH LLNIAHRDLK PENLLFKDNS LDAPVKLCDF GFAKVDQGDL 180 | | MTPQFTPYYV APQVLEAQRR HQKEKSGIIP TSPTPYTYNK SCDLWSLGVI IYVMLCGYPP 240 | | FYSKHHSRTI PKDMRKKIMT GSFEFPEEEW SQISEMAKDV VRKLLKVKPE ERLTIEGVLD 300 | | HPWLNSTEAL DNVLPSAQLM MDKAVVAGIQ QAHAEQLANM RIQDLKVSLK PLHSVNNPIL 360 | | RKRKLLGRE 369 |
|
Nucleotide Sequence
(FASTA) | | ATGTCGGAGG ACAGCGACAT GGAGAAAGCC ATCAAGGAGA CCTCCATTTT AGAAGAATAT 60 | | AGTATCAATT GGACTCAGAA ACTGGGAGCC GGAATTAGTG GTCCAGTTAG AGTCTGTGTG 120 | | AAGAAATCCA CTCAAGAACG GTTTGCACTG AAAATTCTTC TTGATCGTCC AAAAGCTAGA 180 | | AATGAGGTGC GCCTGCACAT GATGTGTGCC ACACACCCCA ACATAGTTCA GATTATTGAA 240 | | GTGTTTGCTA ACAGTGTACA GTTCCCTCAT GAGTCCAGCC CCAGGGCTCG ACTCTTAATT 300 | | GTAATGGAGA TGATGGAAGG GGGAGAGCTA TTTCACAGAA TCAGCCAGCA CCGGCACTTT 360 | | ACAGAGAAGC AAGCCAGCCA AGTAACAAAG CAGATAGCCC TGGCTCTACA GCACTGTCAC 420 | | TTGCTAAACA TTGCGCACAG AGACCTCAAG CCTGAAAATC TGCTTTTCAA GGATAACTCT 480 | | CTGGACGCCC CTGTGAAATT ATGTGACTTT GGGTTTGCTA AAGTTGACCA AGGTGATTTG 540 | | ATGACACCCC AGTTTACCCC TTACTATGTA GCACCTCAGG TACTGGAAGC GCAGAGACGG 600 | | CACCAGAAGG AGAAGTCTGG CATCATACCT ACCTCGCCAA CACCCTACAC TTACAACAAG 660 | | AGCTGTGACT TGTGGTCCCT AGGGGTGATA ATTTATGTGA TGCTGTGCGG ATATCCTCCT 720 | | TTTTACTCCA AACACCATAG TCGGACTATC CCAAAGGATA TGCGGAAAAA GATCATGACA 780 | | GGAAGTTTCG AGTTCCCAGA AGAAGAGTGG AGCCAGATCT CAGAGATGGC TAAAGATGTT 840 | | GTGAGGAAGC TTCTGAAGGT CAAACCAGAG GAAAGACTCA CAATCGAGGG AGTGTTGGAC 900 | | CATCCCTGGC TCAACTCGAC AGAGGCCCTG GATAATGTGC TACCCTCTGC CCAGCTGATG 960 | | ATGGATAAGG CGGTGGTTGC GGGGATCCAG CAGGCGCACG CCGAGCAGCT GGCAAACATG 1020 | | AGGATCCAGG ACCTCAAGGT CAGCCTCAAA CCCCTGCACT CTGTCAACAA CCCCATTCTC 1080 | | AGGAAGAGGA AGCTGCTGGG GAGAGAATGA 1110 |
|
| Domain Profile | S: 2 lteevlGeGisgkvlecvkkktgekyalkilkdrkkvrrevelhvqasghknivelidvf 61 | +++++lG+Gisg v+ cvkk+t+e++alkil dr+k+r+ev+lh+++++h+niv++i+vf | Q: 23 NWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHPNIVQIIEVF 82 | 689********************************************************* |
| S: 62 ensfe......dekrlllvlekleGGellsriqerkaftekeasevvkdiasalkflhsk 115 | +ns++ +++rll+v+e++eGGel++ri+++++ftek+as+v+k+ia al+++h | Q: 83 ANSVQfphessPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLL 142 | ****99999999************************************************ |
| S: 116 niahrDlkpenlLyeskeknapvklcDfglaketeeeeelktpcltaeyvaPevleaeke 175 | niahrDlkpenlL+++++ +apvklcDfg+ak ++ +l+tp++t++yvaP+vlea+++ | Q: 143 NIAHRDLKPENLLFKDNSLDAPVKLCDFGFAK--VDQGDLMTPQFTPYYVAPQVLEAQRR 200 | ********************************..89***********************9 |
| S: 176 e.............allydksCDlWslGvilyillcGypPfyskk.graiskklkeriqe 221 | + +++y+ksCDlWslGvi+y++lcGypPfysk+ +r+i+k+++++i++ | Q: 201 HqkeksgiiptsptPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHhSRTIPKDMRKKIMT 260 | 99*****************************************9979************* |
| S: 222 gkyefPeeeWsevseeakdlirklLkrdpkerltieevlnhpwv 265 | g++efPeeeWs++se+akd++rklLk++p+erltie vl+hpw+ | Q: 261 GSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWL 304 | *******************************************6 |
|
Domain Sequence
(FASTA) | | NWTQKLGAGI SGPVRVCVKK STQERFALKI LLDRPKARNE VRLHMMCATH PNIVQIIEVF 60 | | ANSVQFPHES SPRARLLIVM EMMEGGELFH RISQHRHFTE KQASQVTKQI ALALQHCHLL 120 | | NIAHRDLKPE NLLFKDNSLD APVKLCDFGF AKVDQGDLMT PQFTPYYVAP QVLEAQRRHQ 180 | | KEKSGIIPTS PTPYTYNKSC DLWSLGVIIY VMLCGYPPFY SKHHSRTIPK DMRKKIMTGS 240 | | FEFPEEEWSQ ISEMAKDVVR KLLKVKPEER LTIEGVLDHP WL 282 |
|
| Keyword | Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tumor suppressor. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
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| Prints | |
| Created Date | 20-Feb-2013 |