EKS-MUM-00476

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00476

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/DMPK	456.6	6.5E-137	71	339	269

Status

Reviewed

Ensembl Protein

ENSMUSP00000104113

UniProt Accession

P54265;

Protein Name

Myotonin-protein kinase

Protein Synonyms/Alias

MT-PK; DM-kinase; DMK; DMPK; Myotonic dystrophy protein kinase; MDPK;

Gene Name

Dmpk

Gene Synonyms/Alias

Dmpk; Dm15, Mdpk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000030409	ENSMUSP00000032568	ENSMUST00000032568
ENSMUSG00000030409	ENSMUSP00000104114	ENSMUST00000108474
ENSMUSG00000030409	ENSMUSP00000104113	ENSMUST00000108473
ENSMUSG00000030409	ENSMUSP00000118459	ENSMUST00000154199
ENSMUSG00000030409	ENSMUSP00000123516	ENSMUST00000122999

Organism

Mus musculus

Functional Description

Non-receptor serine/threonine protein kinase which isnecessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.

Protein Length

537

Protein Sequence
(FASTA)

MSAEVRLRQL QQLVLDPGFL GLEPLLDLLL GVHQELGASH LAQDKYVADF LQWVEPIAAR 60

LKEVRLQRDD FEILKVIGRG AFSEVAVVKM KQTGQVYAMK IMNKWDMLKR GEVSCFREER 120

DVLVKGDRRW ITQLHFAFQD ENYLYLVMEY YVGGDLLTLL SKFGERIPAE MARFYLAEIV 180

MAIDSVHRLG YVHRDIKPDN ILLDRCGHIR LADFGSCLKL QPDGMVRSLV AVGTPDYLSP 240

EILQAVGGGP GAGSYGPECD WWALGVFAYE MFYGQTPFYA DSTAETYAKI VHYREHLSLP 300

LADTVVPEEA QDLIRGLLCP AEIRLGRGGA GDFQKHPFFF GLDWEGLRDS VPPFTPDFEG 360

ATDTCNFDVV EDRLTAMVSG GGETLSDMQE DMPLGVRLPF VGYSYCCMAF RDNQVPDPTP 420

MELEALQLPV SDLQGLDLQP PVSPPDQVAE EADLVAVPAP VAEAETTVTL QQLQEALEEE 480

VLTRQSLSRE LEAIRTANQN FSSQLQEAEV RNRDLEAHVR QLQERMEMLQ APGAADP 537

Nucleotide Sequence
(FASTA)

ATGTCAGCCG AAGTGCGGCT GAGGCAGCTC CAGCAGCTGG TGCTGGACCC AGGCTTCCTG 60

GGACTGGAGC CCCTGCTCGA CCTTCTCCTG GGCGTCCACC AGGAGCTGGG TGCCTCTCAC 120

CTAGCCCAGG ACAAGTATGT GGCCGACTTC TTGCAGTGGG TGGAGCCCAT TGCAGCAAGG 180

CTTAAGGAGG TCCGACTGCA GAGGGATGAT TTTGAGATTT TGAAGGTGAT CGGGCGTGGG 240

GCGTTCAGCG AGGTAGCGGT GGTGAAGATG AAACAGACGG GCCAAGTGTA TGCCATGAAG 300

ATTATGAATA AGTGGGACAT GCTGAAGAGA GGCGAGGTGT CGTGCTTCCG GGAAGAAAGG 360

GATGTATTAG TGAAAGGGGA CCGGCGCTGG ATCACACAGC TGCACTTTGC CTTCCAGGAT 420

GAGAACTACC TGTACCTGGT CATGGAATAC TACGTGGGCG GGGACCTGCT AACGCTGCTG 480

AGCAAGTTTG GGGAGCGGAT CCCCGCCGAG ATGGCTCGCT TCTACCTGGC CGAGATTGTC 540

ATGGCCATAG ACTCCGTGCA CCGGCTGGGC TACGTGCACA GGGACATCAA ACCAGATAAC 600

ATTCTGCTGG ACCGATGTGG GCACATTCGC CTGGCAGACT TCGGCTCCTG CCTCAAACTG 660

CAGCCTGATG GAATGGTGAG GTCGCTGGTG GCTGTGGGCA CCCCGGACTA CCTGTCTCCT 720

GAGATTCTGC AGGCCGTTGG TGGAGGGCCT GGGGCAGGCA GCTACGGGCC AGAGTGTGAC 780

TGGTGGGCAC TGGGCGTGTT CGCCTATGAG ATGTTCTATG GGCAGACCCC CTTCTACGCG 840

GACTCCACAG CCGAGACATA TGCCAAGATT GTGCACTACA GGGAACACTT GTCGCTGCCG 900

CTGGCAGACA CAGTTGTCCC CGAGGAAGCT CAGGACCTCA TTCGTGGGCT GCTGTGTCCT 960

GCTGAGATAA GGCTAGGTCG AGGTGGGGCA GGTGATTTCC AGAAACATCC TTTCTTCTTT 1020

GGCCTTGATT GGGAGGGTCT CCGAGACAGT GTACCCCCCT TTACACCAGA CTTCGAGGGT 1080

GCCACGGACA CATGCAATTT CGATGTGGTG GAGGACCGGC TCACTGCCAT GGTGAGCGGG 1140

GGCGGGGAGA CGCTGTCAGA CATGCAGGAA GACATGCCCC TTGGGGTGCG CCTGCCCTTC 1200

GTGGGCTACT CCTACTGCTG CATGGCCTTC AGAGACAATC AGGTCCCGGA CCCCACCCCT 1260

ATGGAACTAG AGGCCCTGCA GTTGCCTGTG TCAGACTTGC AAGGGCTTGA CTTGCAGCCC 1320

CCAGTGTCCC CACCGGATCA AGTGGCTGAA GAGGCTGACC TAGTGGCTGT CCCTGCCCCT 1380

GTGGCTGAGG CAGAGACCAC GGTAACGCTG CAGCAGCTCC AGGAAGCCCT GGAAGAAGAG 1440

GTTCTCACCC GGCAGAGCCT GAGCCGCGAG CTGGAGGCCA TCCGGACCGC CAACCAGAAC 1500

TTCTCCAGCC AACTACAGGA GGCCGAGGTC CGAAACCGAG ACCTGGAGGC GCATGTTCGG 1560

CAGCTACAGG AACGGATGGA GATGCTGCAG GCCCCAGGAG CCGCAGATCC CTAG 1614

Domain Profile

S: 1     feilkviGrGafgevavvrekatekvyamkilkklellkraesaffreerdvlvkadsew  60

         feilkviGrGaf+evavv++k+t++vyamki++k+++lkr e+++freerdvlvk+d++w

Q: 71    FEILKVIGRGAFSEVAVVKMKQTGQVYAMKIMNKWDMLKRGEVSCFREERDVLVKGDRRW  130

         89**********************************************************

S: 61    ivqlqyafqdekylylvmeylvGGdlltllskyedklpeelarfylaelvlaldsvhelg  120

         i+ql++afqde+ylylvmey+vGGdlltllsk+++++p+e+arfylae+v+a+dsvh+lg

Q: 131   ITQLHFAFQDENYLYLVMEYYVGGDLLTLLSKFGERIPAEMARFYLAEIVMAIDSVHRLG  190

         ************************************************************

S: 121   yvhrdikpdnilldksGhikladfGsclklnedgtvkssvavGtpdyispevlkalee..  178

         yvhrdikpdnilld++Ghi+ladfGsclkl+ dg+v+s vavGtpdy+spe+l+a++

Q: 191   YVHRDIKPDNILLDRCGHIRLADFGSCLKLQPDGMVRSLVAVGTPDYLSPEILQAVGGgp  250

         ********************************************************9978

S: 179   gkgeygkecdwwslGvilyellvgetpfyaeslvetyskilnhkeslkfpede.kvseea  237

         g+g+yg+ecdww+lGv++ye+++g+tpfya+s++ety+ki++++e+l++p  +  v+eea

Q: 251   GAGSYGPECDWWALGVFAYEMFYGQTPFYADSTAETYAKIVHYREHLSLPLADtVVPEEA  310

         899***********************************************766599****

S: 238   kdlikklltsreerlgregiedlkkhpff  266

         +dli+ ll+ +e rlgr g  d++khpff

Q: 311   QDLIRGLLCPAEIRLGRGGAGDFQKHPFF  339

         ****************************9

Domain Sequence
(FASTA)

FEILKVIGRG AFSEVAVVKM KQTGQVYAMK IMNKWDMLKR GEVSCFREER DVLVKGDRRW 60

ITQLHFAFQD ENYLYLVMEY YVGGDLLTLL SKFGERIPAE MARFYLAEIV MAIDSVHRLG 120

YVHRDIKPDN ILLDRCGHIR LADFGSCLKL QPDGMVRSLV AVGTPDYLSP EILQAVGGGP 180

GAGSYGPECD WWALGVFAYE MFYGQTPFYA DSTAETYAKI VHYREHLSLP LADTVVPEEA 240

QDLIRGLLCP AEIRLGRGGA GDFQKHPFF 269

Keyword

Alternative splicing; ATP-binding; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Endoplasmic reticulum; Kinase; Magnesium; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Sarcoplasmic reticulum; Serine/threonine-protein kinase; Transferase; Transmembrane; Transmembrane helix.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005789	; C:endoplasmic reticulum membrane
GO:0031307	; C:integral to mitochondrial outer membrane
GO:0031965	; C:nuclear membrane
GO:0005640	; C:nuclear outer membrane
GO:0005886	; C:plasma membrane
GO:0033017	; C:sarcoplasmic reticulum membrane
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0017020	; F:myosin phosphatase regulator activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006874	; P:cellular calcium ion homeostasis
GO:0010657	; P:muscle cell apoptotic process
GO:0006998	; P:nuclear envelope organization
GO:0014853	; P:regulation of excitatory postsynaptic membrane potential involved in skeletal muscle contraction
GO:0008016	; P:regulation of heart contraction
GO:0010830	; P:regulation of myotube differentiation
GO:0014722	; P:regulation of skeletal muscle contraction by calcium ion signaling
GO:0002028	; P:regulation of sodium ion transport
GO:0051823	; P:regulation of synapse structural plasticity

KEGG

mmu:13400

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR014930	; Myotonic_dystrophy_kinase_coil.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF08826	; DMPK_coil; 1.
PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013