EKS-MUM-00417

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00417

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	263.3	5.6E-78	195	445	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000033182

UniProt Accession

O55222; Q78KK2;

Protein Name

Integrin-linked protein kinase

Protein Synonyms/Alias

Gene Name

Ilk

Gene Synonyms/Alias

Ilk;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000030890	ENSMUSP00000130341	ENSMUST00000163389
ENSMUSG00000030890	ENSMUSP00000123443	ENSMUST00000136687
ENSMUSG00000030890	ENSMUSP00000033182	ENSMUST00000033182
ENSMUSG00000030890	ENSMUSP00000116329	ENSMUST00000127298

Organism

Mus musculus

Functional Description

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (By similarity).

Protein Length

452

Protein Sequence
(FASTA)

MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI 60

NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV 120

ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG 180

TLNKHSGIDF KQLNFLAKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP 240

RLRIFSHPNV LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD 300

MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA 360

LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH 420

VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK 452

Nucleotide Sequence
(FASTA)

ATGGACGACA TTTTCACTCA GTGCCGGGAG GGCAACGCGG TGGCGGTGCG CTTGTGGCTG 60

GACAACACAG AGAACGACCT CAATCAGGGG GATGATCATG GCTTCTCCCC CTTGCACTGG 120

GCCTGCCGAG AAGGCCGCTC TGCGGTGGTT GAAATGCTGA TCATGCGTGG AGCACGGATT 180

AATGTGATGA ATCGTGGGGA TGATACCCCC CTGCACCTGG CAGCTAGTCA TGGACACCGT 240

GACATTGTAC AGAAGCTGTT GCAATACAAG GCTGACATCA ATGCAGTGAA TGAGCACGGC 300

AATGTGCCAC TTCACTATGC ATGTTTCTGG GGCCAAGACC AGGTGGCAGA GGACCTGGTG 360

GCTAACGGGG CTCTTGTGAG CATCTGTAAC AAGTATGGAG AGATGCCTGT GGACAAAGCC 420

AAGGCACCCC TTAGAGAGCT TCTCCGAGAA CGGGCAGAGA AAATGGGCCA GAATCTCAAC 480

CGTATTCCAT ACAAGGACAC ATTCTGGAAG GGGACCACTC GCACAAGGCC CCGAAATGGG 540

ACCCTGAACA AACACTCCGG TATTGACTTC AAACAGCTCA ACTTTCTGGC AAAGCTCAAT 600

GAGAATCATT CTGGAGAGCT TTGGAAAGGC CGCTGGCAGG GCAATGATAT TGTTGTGAAG 660

GTGCTGAAGG TTCGAGACTG GAGTACAAGG AAAAGCAGGG ACTTCAATGA GGAATGTCCC 720

CGGCTCAGGA TTTTCTCACA CCCTAACGTG CTCCCAGTGC TAGGTGCTTG CCAGGCTCCC 780

CCAGCCCCCC ACCCAACCCT CATCACACAC TGGATGCCAT ATGGATCTCT CTACAATGTT 840

CTACATGAAG GCACCAATTT CGTTGTGGAC CAGAGCCAAG CTGTAAAGTT TGCTTTGGAC 900

ATGGCAAGAG GCATGGCTTT TCTTCACACA CTAGAGCCTC TCATACCCCG GCATGCACTC 960

AATAGCCGCA GTGTAATGAT CGATGAAGAT ATGACTGCCC GAATCAGCAT GGCTGATGTT 1020

AAGTTTTCTT TCCAGTGCCC TGGGCGCATG TATGCGCCTG CCTGGGTGGC CCCTGAAGCC 1080

CTGCAGAAGA AGCCTGAAGA CACAAACAGA CGCTCAGCAG ACATGTGGAG TTTCGCGGTG 1140

CTTCTGTGGG AACTGGTGAC ACGAGAGGTG CCCTTTGCTG ACCTCTCTAA TATGGAGATT 1200

GGGATGAAGG TGGCACTGGA AGGCCTTCGG CCTACCATCC CACCAGGTAT TTCCCCCCAT 1260

GTGTGTAAGC TCATGAAGAT TTGCATGAAT GAAGACCCTG CAAAGCGACC CAAGTTTGAC 1320

ATGATTGTGC CTATCTTGGA GAAGATGCAG GACAAGTAG 1359

Domain Profile

S: 3     leeligsgafgkvykakwrkkevavKklreeaeekekleklereakllsklshpniiell  62

         +  +++++++g+++k++w++++++vK l++++++++k++++++e+ +l+ +shpn++ +l

Q: 195   FLAKLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVL  254

         667899******************************************************

S: 63    Gvcleepn..lllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseapk  120

         G+c+++p+   +l+++++++gsL+nvlhe ++ ++d++++v++al++ArGma+Lh+ +p

Q: 255   GACQAPPAphPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPL  314

         ******99745**********************************************997

S: 121   kiihrdLksknvLlseekktlkicdFGtarelskstkmsaa.gtlawmaPEvlkeekvs.  178

          i ++ L+s++v++ +e++t++i++ +++++   s+++  + +++aw+aPE+l++++ +

Q: 315   -IPRHALNSRSVMI-DEDMTARISM-ADVKF---SFQCPGRmYAPAWVAPEALQKKPEDt  368

         .*************.**********.88888...78999998**************9998

S: 179   ..eksDvwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkLmkec  236

           +++D+wS++v+LWel+trevP++dls+++i +kval+glr++ip+ ++++++kLmk c

Q: 369   nrRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKIC  428

         8899********************************************************

S: 237   wnedpekRpsfseivks  253

         +nedp+kRp+f++iv +

Q: 429   MNEDPAKRPKFDMIVPI  445

         **************986

Domain Sequence
(FASTA)

FLAKLNENHS GELWKGRWQG NDIVVKVLKV RDWSTRKSRD FNEECPRLRI FSHPNVLPVL 60

GACQAPPAPH PTLITHWMPY GSLYNVLHEG TNFVVDQSQA VKFALDMARG MAFLHTLEPL 120

IPRHALNSRS VMIDEDMTAR ISMADVKFSF QCPGRMYAPA WVAPEALQKK PEDTNRRSAD 180

MWSFAVLLWE LVTREVPFAD LSNMEIGMKV ALEGLRPTIP PGISPHVCKL MKICMNEDPA 240

KRPKFDMIVP I 251

Keyword

ANK repeat; ATP-binding; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005911	; C:cell-cell junction
GO:0043034	; C:costamere
GO:0043198	; C:dendritic shaft
GO:0005925	; C:focal adhesion
GO:0030027	; C:lamellipodium
GO:0043025	; C:neuronal cell body
GO:0005886	; C:plasma membrane
GO:0043234	; C:protein complex
GO:0001725	; C:stress fiber
GO:0043195	; C:terminal button
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004871	; F:signal transducer activity
GO:0001658	; P:branching involved in ureteric bud morphogenesis
GO:0007569	; P:cell aging
GO:0007050	; P:cell cycle arrest
GO:0045197	; P:establishment or maintenance of epithelial cell apical/basal polarity
GO:0043206	; P:fibril organization
GO:0007229	; P:integrin-mediated signaling pathway
GO:0032288	; P:myelin assembly
GO:0010667	; P:negative regulation of cardiac muscle cell apoptotic process
GO:0043524	; P:negative regulation of neuron apoptotic process
GO:0006469	; P:negative regulation of protein kinase activity
GO:0014912	; P:negative regulation of smooth muscle cell migration
GO:0048662	; P:negative regulation of smooth muscle cell proliferation
GO:0048812	; P:neuron projection morphogenesis
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0045773	; P:positive regulation of axon extension
GO:0030513	; P:positive regulation of BMP signaling pathway
GO:0090263	; P:positive regulation of canonical Wnt receptor signaling pathway
GO:0030335	; P:positive regulation of cell migration
GO:0008284	; P:positive regulation of cell proliferation
GO:0001954	; P:positive regulation of cell-matrix adhesion
GO:0050775	; P:positive regulation of dendrite morphogenesis
GO:0043406	; P:positive regulation of MAP kinase activity
GO:0043410	; P:positive regulation of MAPK cascade
GO:0045663	; P:positive regulation of myoblast differentiation
GO:0045669	; P:positive regulation of osteoblast differentiation
GO:0051897	; P:positive regulation of protein kinase B signaling cascade
GO:0045893	; P:positive regulation of transcription, DNA-dependent
GO:0051291	; P:protein heterooligomerization
GO:0032956	; P:regulation of actin cytoskeleton organization
GO:0034446	; P:substrate adhesion-dependent cell spreading

KEGG

mmu:16202

;

InterPros

IPR002110	; Ankyrin_rpt.
IPR020683	; Ankyrin_rpt-contain_dom.
IPR016253	; Integrin-linked_kinase.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.

Pfam

PF00023	; Ank; 3.
PF07714	; Pkinase_Tyr; 1.

SMARTs

SM00248

; ANK; 3.

Prosites

PS50297	; ANK_REP_REGION; 1.
PS50088	; ANK_REPEAT; 3.
PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; FALSE_NEG.

Prints

Created Date

20-Feb-2013