EKS-MUM-00344

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00344

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE20	353.3	2.0E-105	268	519	252

Status

Reviewed

Ensembl Protein

ENSMUSP00000108485

UniProt Accession

Q61036; O88645; Q8K1R5; Q8K1R6;

Protein Name

Serine/threonine-protein kinase PAK 3

Protein Synonyms/Alias

Beta-PAK; CDC42/RAC effector kinase PAK-B; p21-activated kinase 3; PAK-3;

Gene Name

Pak3

Gene Synonyms/Alias

Pak3; Pak-3, Pakb, Stk4;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000031284	ENSMUSP00000108486	ENSMUST00000112865
ENSMUSG00000031284	ENSMUSP00000108485	ENSMUST00000112864
ENSMUSG00000031284	ENSMUSP00000033640	ENSMUST00000033640
ENSMUSG00000031284	ENSMUSP00000108484	ENSMUST00000112863
ENSMUSG00000031284	ENSMUSP00000126562	ENSMUST00000172330
ENSMUSG00000031284	ENSMUSP00000114352	ENSMUST00000126592
ENSMUSG00000031284	ENSMUSP00000108489	ENSMUST00000112868
ENSMUSG00000031284	ENSMUSP00000118549	ENSMUST00000155215
ENSMUSG00000031284	ENSMUSP00000119090	ENSMUST00000134402
ENSMUSG00000031284	ENSMUSP00000118716	ENSMUST00000156449

Organism

Mus musculus

Functional Description

Serine/threonine protein kinase that plays a role in avariety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments.

Protein Length

544

Protein Sequence
(FASTA)

MSDSLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN 60

KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTGIPEQWAR LLQTSNITKL EQKKNPQAVL 120

DVLKFYDSKE TVNNQKYMSF TSGDKSAHGY IAAHQSNTKT ASEPPLAPPV SEEEDEEEEE 180

EEDDNEPPPV IAPRPEHTKS IYTRSVVESI ASPAAPNKED IPPSAENANS TTLYRNTDRQ 240

RKKSKMTDEE ILEKLRSIVS VGDPKKKYTR FEKIGQGASG TVYTALDIAT GQEVAIKQMN 300

LQQQPKKELI INEILVMREN KNPNIVNYLD SYLVGDELWV VMEYLAGGSL TDVVTETCMD 360

EGQIAAVCRE CLQALDFLHS NQVIHRDIKS DNILLGMDGS VKLTDFGFCA QITPEQSKRS 420

TMVGTPYWMA PEVVTRKAYG PKVDIWSLGI MAIEMVEGEP PYLNENPLRA LYLIATNGTP 480

ELQNPERLSA VFRDFLNRCL EMDVDRRGSA KELLQHPFLK LAKPLSSLTP LIIAAKEAIK 540

NSSR 544

Nucleotide Sequence
(FASTA)

ATGTCTGACA GCTTGGATAA CGAAGAAAAA CCCCCAGCTC CCCCACTGAG GATGAACAGT 60

AACAACCGAG ACTCTTCAGC ACTCAACCAC AGCTCCAAAC CACTGCCCAT GGCCCCGGAA 120

GAGAAGAATA AGAAAGCCAG GCTTCGCTCT ATCTTCCCAG GAGGAGGGGA TAAAACCAAT 180

AAGAAGAAAG AGAAAGAGCG CCCAGAGATC TCTCTTCCTT CAGACTTTGA GCATACGATT 240

CATGTGGGTT TTGATGCAGT CACCGGGGAA TTCACTGGAA TTCCTGAGCA ATGGGCACGA 300

CTACTCCAAA CCTCCAACAT AACAAAGCTG GAACAGAAGA AGAACCCACA AGCTGTTCTT 360

GATGTTCTCA AGTTCTACGA CTCCAAAGAA ACGGTCAACA ACCAGAAATA TATGAGCTTT 420

ACGTCAGGAG ATAAAAGTGC CCATGGATAC ATAGCAGCAC ATCAGTCGAA TACCAAAACA 480

GCTTCGGAAC CTCCTTTGGC TCCTCCTGTA TCTGAAGAAG AGGATGAAGA AGAGGAAGAG 540

GAAGAAGATG ACAATGAACC TCCGCCTGTC ATTGCACCAA GACCAGAGCA TACAAAATCA 600

ATCTATACTC GCTCCGTGGT TGAGTCAATT GCTTCACCAG CTGCACCAAA TAAAGAAGAT 660

ATCCCACCTT CTGCTGAGAA TGCCAATTCC ACCACTTTGT ACAGGAATAC AGATCGGCAA 720

AGAAAAAAGT CCAAGATGAC GGATGAGGAG ATCCTAGAGA AGCTGAGAAG CATTGTGAGT 780

GTTGGGGACC CAAAGAAGAA ATATACGAGA TTTGAAAAAA TTGGCCAAGG GGCATCAGGA 840

ACTGTTTACA CAGCACTAGA CATTGCGACT GGACAAGAGG TGGCCATAAA GCAAATGAAC 900

CTTCAACAGC AGCCCAAAAA GGAATTAATT ATTAATGAAA TTCTTGTCAT GAGGGAAAAT 960

AAGAACCCCA ATATTGTCAA TTATTTAGAT AGCTACTTAG TGGGTGATGA ACTGTGGGTA 1020

GTCATGGAAT ACTTGGCTGG TGGCTCTTTG ACTGATGTGG TCACAGAAAC CTGTATGGAT 1080

GAAGGACAGA TAGCAGCTGT CTGTAGAGAG TGCCTCCAAG CTTTGGATTT CTTGCACTCA 1140

AACCAAGTGA TCCACAGAGA CATAAAGAGC GACAACATTC TCCTCGGGAT GGATGGTTCT 1200

GTTAAACTGA CTGATTTTGG GTTCTGTGCT CAAATCACTC CTGAGCAAAG TAAACGAAGC 1260

ACTATGGTGG GAACTCCCTA TTGGATGGCA CCTGAAGTGG TAACTCGAAA AGCTTATGGT 1320

CCAAAAGTTG ATATCTGGTC TCTGGGAATC ATGGCCATTG AAATGGTGGA AGGTGAACCC 1380

CCTTACCTTA ATGAAAATCC ACTCAGGGCC TTATATCTGA TAGCCACTAA TGGGACCCCA 1440

GAGCTCCAGA ATCCTGAGAG ACTGTCAGCT GTATTCCGTG ACTTCTTAAA TCGCTGTCTT 1500

GAGATGGATG TGGATAGAAG AGGATCTGCT AAGGAGCTTT TGCAGCATCC GTTTTTAAAA 1560

TTAGCCAAGC CTCTGTCTAG TCTCACTCCT CTGATTATCG CCGCAAAGGA AGCAATTAAG 1620

AACAGTAGCC GTTAG 1635

Domain Profile

S: 1     yelleeigkGalgkvylarekktgelvaikkinlekqekleelqkeisvlkklkhpnive  60

         y+ +e+ig+Ga+g+vy+a ++ tg+ vaik++nl++q+k+e++++ei v+++ k+pniv+

Q: 268   YTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVN  327

         7889********************************************************

S: 61    ylesflkeeelwlvmeylagGslsdlvkeelkselkEeqiayilrevlkaLeylHskkvi  120

         yl+s+l+ +elw+vmeylagGsl+d+v+   ++ ++E qia+++re+l+aL++lHs++vi

Q: 328   YLDSYLVGDELWVVMEYLAGGSLTDVVT---ETCMDEGQIAAVCRECLQALDFLHSNQVI  384

         ****************************...9****************************

S: 121   HrdikakniLlteegeVkLsdfgvaaqlektrkkrktvvgtpywmAPEviaqeekgYdek  180

         Hrdik++niLl ++g+VkL+dfg++aq++ +++kr+t+vgtpywmAPEv++  +k Y  k

Q: 385   HRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVT--RKAYGPK  442

         ***************************************************..9******

S: 181   aDiwslGItaielaegepPfkdleplkallkilkneppklkskkkfskefkefvekclqk  240

         +DiwslGI+aie++egepP+ +++pl+al++i++n +p+l++++++s  f++f+++cl+

Q: 443   VDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEM  502

         ************************************************************

S: 241   npekRpsasellkhkFl  257

         ++++R sa+ell+h+Fl

Q: 503   DVDRRGSAKELLQHPFL  519

         ****************7

Domain Sequence
(FASTA)

YTRFEKIGQG ASGTVYTALD IATGQEVAIK QMNLQQQPKK ELIINEILVM RENKNPNIVN 60

YLDSYLVGDE LWVVMEYLAG GSLTDVVTET CMDEGQIAAV CRECLQALDF LHSNQVIHRD 120

IKSDNILLGM DGSVKLTDFG FCAQITPEQS KRSTMVGTPY WMAPEVVTRK AYGPKVDIWS 180

LGIMAIEMVE GEPPYLNENP LRALYLIATN GTPELQNPER LSAVFRDFLN RCLEMDVDRR 240

GSAKELLQHP FL 252

Keyword

3D-structure; Acetylation; Allosteric enzyme; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Developmental protein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; SH3-binding; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005524	; F:ATP binding
GO:0004708	; F:MAP kinase kinase activity
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007409	; P:axonogenesis
GO:0016358	; P:dendrite development
GO:0030833	; P:regulation of actin filament polymerization

KEGG

mmu:18481

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000095	; PAK_box_Rho-bd.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00786	; PBD; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00285	; PBD; 1.
SM00220	; S_TKc; 1.

Prosites

PS50108	; CRIB; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013