EKS-MUM-00111

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00111

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Tec	479.7	4.2E-144	393	643	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000107882

UniProt Accession

P97504;

Protein Name

Cytoplasmic tyrosine-protein kinase BMX

Protein Synonyms/Alias

Bone marrow tyrosine kinase gene in chromosome X protein homolog;

Gene Name

Bmx

Gene Synonyms/Alias

Bmx;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000031377	ENSMUSP00000107884	ENSMUST00000112265
ENSMUSG00000031377	ENSMUSP00000107882	ENSMUST00000112263

Organism

Mus musculus

Functional Description

Non-receptor tyrosine kinase that plays central butdiverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.

Protein Length

651

Protein Sequence
(FASTA)

MESKSILEEL LLKKSQQKKK MSPNNYKERL FVLTKTSLSY YEYDKMKRGS RKGSIEIKKI 60

RCVEKVNLEE QTPVERQYPF QIVYKDGLLY VYASNEESRC QWLKALQKEI RGNPHLLIKY 120

HSGFFVDGKF LCCQQSCKAA PGCTLWEAYA DLHIAISDEK HRAPTFPERL LKIPRAVPVL 180

KMDASSSGAI LPQYDSYSKK SCGSQPTSNI RYIPREDCPD WWQVRKLKSE EDIACSNQLE 240

RNIASHSTSK MSWGFPESSS SEEEENLHAY DWFAGNISRS QSEQLLRQKG KEGAFMVRNS 300

SQMGMYTVSL FSKAVNDKKG TVKHYHVHTN AENKLYLAEN YCFDSIPKLI HYHQHNSAGM 360

ITRLRHPVST KANKVPVSVA LGSGIWELKR EEITLLKELG NGQFGVVQLG QWKGQYDVAV 420

KMIKEGAMSE DEFFQEAQTM MKLSHPKLVK FYGVCSKKYP IYIVTEYITN GCLLNYLKSH 480

GKGLESCQLL EMCYDVCEGM AFLESHQFIH RDLAARNCLV DSDLSVKVSD FGMTRYVLDD 540

QYVSSVGTKF PVKWSAPEVF HYFKYSSKSD VWAFGILMWE VFSLGKQPYD LYDNSEVVVK 600

VSQGHRLYRP QLASDTIYQI MYSCWHELPE KRPTFQQLLS AIEPLREQDK P 651

Nucleotide Sequence
(FASTA)

ATGGAGAGTA AATCCATTCT GGAAGAACTG CTTCTCAAAA AGTCACAGCA AAAGAAGAAA 60

ATGTCACCAA ATAATTACAA GGAGCGGCTT TTTGTTCTAA CCAAAACAAG CCTTTCCTAC 120

TATGAATACG ACAAAATGAA AAGAGGAAGC AGAAAAGGAT CAATAGAAAT TAAGAAAATC 180

AGATGTGTGG AAAAAGTCAA CCTTGAGGAG CAGACACCGG TGGAGAGACA GTACCCATTT 240

CAGATTGTGT ATAAAGATGG GCTTCTTTAT GTCTATGCAT CGAATGAAGA GAGCCGATGT 300

CAGTGGTTAA AAGCGCTGCA AAAAGAGATA CGGGGCAACC CTCACCTGTT GATCAAGTAT 360

CACAGTGGCT TCTTTGTGGA TGGAAAGTTC CTGTGTTGCC AGCAGAGCTG CAAAGCAGCC 420

CCAGGATGCA CTCTCTGGGA AGCATATGCT GATCTGCACA TCGCAATCAG TGATGAGAAA 480

CACAGAGCTC CCACTTTCCC AGAGAGGCTA CTGAAGATTC CAAGGGCAGT TCCCGTTCTC 540

AAAATGGATG CATCATCTTC AGGTGCCATT CTACCCCAAT ATGACAGCTA TTCAAAGAAA 600

AGTTGTGGTT CCCAGCCAAC CAGCAACATA CGCTATATTC CAAGGGAAGA CTGCCCTGAC 660

TGGTGGCAAG TAAGAAAACT GAAAAGCGAG GAAGACATTG CATGCAGTAA CCAACTGGAA 720

AGAAATATCG CCTCTCACAG CACCTCAAAG ATGTCATGGG GATTCCCTGA GTCAAGTTCA 780

TCAGAAGAAG AGGAAAATCT GCATGCTTAT GACTGGTTTG CTGGGAATAT CTCCAGGTCA 840

CAATCTGAGC AGTTACTGAG ACAAAAGGGA AAAGAAGGAG CATTTATGGT TCGGAATTCC 900

AGCCAGATGG GAATGTACAC TGTGTCCTTA TTTAGTAAGG CTGTAAATGA TAAAAAAGGA 960

ACTGTCAAGC ATTACCACGT GCATACTAAT GCTGAAAATA AATTATACCT GGCTGAAAAC 1020

TACTGCTTTG ATTCCATTCC AAAGCTCATT CACTATCACC AACATAATTC AGCAGGCATG 1080

ATCACACGGC TCCGCCACCC AGTGTCAACC AAGGCCAACA AGGTCCCGGT GTCTGTGGCT 1140

CTGGGAAGTG GAATTTGGGA ACTGAAAAGA GAAGAGATTA CCTTGCTGAA GGAGCTGGGC 1200

AATGGCCAGT TTGGAGTGGT CCAGCTGGGC CAGTGGAAGG GGCAATATGA TGTGGCTGTA 1260

AAGATGATCA AGGAGGGTGC CATGTCAGAA GATGAATTCT TTCAGGAGGC CCAGACCATG 1320

ATGAAACTCA GCCATCCCAA GCTGGTGAAG TTCTACGGAG TATGCTCAAA GAAATACCCC 1380

ATCTATATAG TAACTGAGTA TATAACAAAT GGTTGCTTGC TTAATTACCT GAAGAGTCAT 1440

GGGAAAGGAC TAGAAAGTTG CCAGCTCTTA GAAATGTGTT ATGATGTCTG TGAAGGCATG 1500

GCCTTCTTGG AGAGCCATCA GTTCATACAT CGAGATTTGG CTGCTCGGAA CTGTTTGGTG 1560

GACAGTGATC TCTCTGTGAA AGTCTCAGAC TTTGGAATGA CGAGATATGT CCTTGATGAT 1620

CAGTATGTCA GCTCAGTAGG AACCAAGTTT CCAGTCAAGT GGTCAGCTCC AGAGGTGTTT 1680

CACTACTTCA AATACAGCAG CAAGTCGGAT GTATGGGCAT TTGGGATCCT GATGTGGGAG 1740

GTGTTTAGTC TAGGGAAGCA GCCCTATGAC TTATATGATA ACTCCGAGGT GGTTGTGAAG 1800

GTCTCCCAGG GCCACAGACT CTACCGGCCC CAACTGGCAT CAGACACCAT CTACCAGATC 1860

ATGTACAGCT GCTGGCATGA GCTTCCAGAA AAGCGCCCCA CATTTCAGCA ACTCCTGTCT 1920

GCCATAGAAC CACTTCGGGA ACAAGATAAA CCATGA 1956

Domain Profile

S: 1     ltllkelgsgqfgvvklgkwrakidvaikaikegamseedfieeakvmmklshpklvqly  60

         +tllkelg+gqfgvv+lg+w++++dva+k+ikegamse++f++ea++mmklshpklv++y

Q: 393   ITLLKELGNGQFGVVQLGQWKGQYDVAVKMIKEGAMSEDEFFQEAQTMMKLSHPKLVKFY  452

         699*********************************************************

S: 61    gvclkqkpiyivteylengcllnylrekkkklekelllemckdvcegmeylerksfihrd  120

         gvc+k++piyivtey++ngcllnyl+++ k le+++llemc+dvcegm++le+++fihrd

Q: 453   GVCSKKYPIYIVTEYITNGCLLNYLKSHGKGLESCQLLEMCYDVCEGMAFLESHQFIHRD  512

         ************************************************************

S: 121   laarnclvdeelvvkvsdfglaryvlddqyvssvgakfpvkwsppevlsyskyssksdvw  180

         laarnclvd++l vkvsdfg++ryvlddqyvssvg+kfpvkws+pev++y+kyssksdvw

Q: 513   LAARNCLVDSDLSVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVW  572

         ************************************************************

S: 181   afgvlmwevfsegklpyekksnsevvekvsrglrlyrpklaskkvyevmkscwkekpeer  240

         afg+lmwevfs+gk+py+ ++nsevv kvs+g+rlyrp+las+++y++m+scw+e pe+r

Q: 573   AFGILMWEVFSLGKQPYDLYDNSEVVVKVSQGHRLYRPQLASDTIYQIMYSCWHELPEKR  632

         ************************************************************

S: 241   ptfkellsale  251

         ptf++llsa+e

Q: 633   PTFQQLLSAIE  643

         ********996

Domain Sequence
(FASTA)

ITLLKELGNG QFGVVQLGQW KGQYDVAVKM IKEGAMSEDE FFQEAQTMMK LSHPKLVKFY 60

GVCSKKYPIY IVTEYITNGC LLNYLKSHGK GLESCQLLEM CYDVCEGMAF LESHQFIHRD 120

LAARNCLVDS DLSVKVSDFG MTRYVLDDQY VSSVGTKFPV KWSAPEVFHY FKYSSKSDVW 180

AFGILMWEVF SLGKQPYDLY DNSEVVVKVS QGHRLYRPQL ASDTIYQIMY SCWHELPEKR 240

PTFQQLLSAI E 251

Keyword

Apoptosis; ATP-binding; Cell adhesion; Complete proteome; Cytoplasm; Kinase; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; SH2 domain; Transferase; Tyrosine-protein kinase; Zinc; Zinc-finger.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0005543	; F:phospholipid binding
GO:0006915	; P:apoptotic process
GO:0007155	; P:cell adhesion
GO:0035556	; P:intracellular signal transduction

KEGG

mmu:12169

;

InterPros

IPR011009	; Kinase-like_dom.
IPR011993	; PH_like_dom.
IPR001849	; Pleckstrin_homology.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR001562	; Znf_Btk_motif.

Pfam

PF00779	; BTK; 1.
PF00169	; PH; 1.
PF07714	; Pkinase_Tyr; 1.
PF00017	; SH2; 1.

SMARTs

SM00107	; BTK; 1.
SM00233	; PH; 1.
SM00252	; SH2; 1.
SM00219	; TyrKc; 1.

Prosites

PS50003	; PH_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; 1.
PS51113	; ZF_BTK; 1.

Prints

PR00401	; SH2DOMAIN.
PR00402	; TECBTKDOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013