EKS-MUM-00379

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00379

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/NEK	370.9	8.9E-111	4	256	253

Status

Reviewed

Ensembl Protein

ENSMUSP00000034065

UniProt Accession

P51954;

Protein Name

Serine/threonine-protein kinase Nek1

Protein Synonyms/Alias

Never in mitosis A-related kinase 1; NimA-related protein kinase 1;

Gene Name

Nek1

Gene Synonyms/Alias

Nek1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000031644	ENSMUSP00000113932	ENSMUST00000120689
ENSMUSG00000031644	ENSMUSP00000034065	ENSMUST00000034065
ENSMUSG00000031644	ENSMUSP00000121479	ENSMUST00000142601

Organism

Mus musculus

Functional Description

Phosphorylates serines and threonines, but also appearsto possess tyrosine kinase activity. Implicated in the control of meiosis. Involved in cilium assembly (By similarity).

Protein Length

1203

Protein Sequence
(FASTA)

MEKYVRLQKI GEGSFGKAVL VKSTEDGRHY VIKEINISRM SDKERQESRR EVAVLANMKH 60

PNIVQYKESF EENGSLYIVM DYCEGGDLFK RINAQKGALF QEDQILDWFV QICLALKHVH 120

DRKILHRDIK SQNIFLTKDG TVQLGDFGIA RVLNSTVELA RTCIGTPYYL SPEICENKPY 180

NNKSDIWALG CVLYELCTLK HAFEAGNMKN LVLKIISGSF PPVSPHYSYD LRSLLSQLFK 240

RNPRDRPSVN SILEKGFIAK RIEKFLSPQL IAEEFCLKTL SKFGPQPLPG KRPASGQGVS 300

SFVPAQKITK PAAKYGVPLT YKKYGDKKLL EKKPPPKHKQ AHQIPVKKMN SGEERKKMSE 360

EAAKKRRLEF IEKEKKQKDQ IRFLKAEQMK RQEKQRLERI NRAREQGWRN VLRAGGSGEV 420

KASFFGIGGA VSPSPCSPRG QYEHYHAIFD QMQRLRAEDN EARWKGGIYG RWLPERQKGH 480

LAVERANQVE EFLQRKREAM QNKARAEGHV VYLARLRQIR LQNFNERQQI KAKLRGENKE 540

ADGTKGQEAT EETDMRLKKM ESLKAQTNAR AAVLKEQLER KRKEAYEREK KVWEEHLVAR 600

VKSSDVPLPL ELLETGGSPS KQQVKPVISV TSALKEVGLD GSLTDTQEEE MEKSNSAISS 660

KREILRRLNE NLKAQEDEKE KQHHSGSCET VGHKDEREYE TENAISSDRK KWEMGGQLVI 720

PLDAVTLDTS FSATEKHTVG EVIKLDSNGS PRKVWGKNPT DSVLKILGEA ELQLQTELLE 780

NTSFKSEVYA EEENYKPLLT EEENLQCISK EINPSATVDS TETKSPKFTE VSPQMSEGNV 840

EEPDDLETEV LQEPSSTHTD GSLPPVLNDV WTREKEAAKE TELEDKVAVQ QSEVCEDRIP 900

GNVDQSCKDQ RDPAVDDSPQ SGCDVEKSVQ PESIFQKVVH SKDLNLVQAV HCSPEEPIPI 960

RSHSDSPPKT KSKNSLLIGL STGLFDANNP KMLRTCSLPD LSKLFRTLMD VPTVGDVHQD 1020

SLEIDELEDE PIKEGPSDSE DTVFEETDTD LQELQASMEQ LLREQPGDEY SEEEESVLKS 1080

SDVEQTARGT DAPDEEDNPS SESALNEEWH SDNSDAETTS ECEYDSVFNH LEELRLHLEQ 1140

EMGFEKFFEV YEKVKAIHED EDENIEICST IVENILGNEH QHLYAKILHL VMADGAYQED 1200

NDE 1203

Nucleotide Sequence
(FASTA)

ATGGAGAAGT ATGTGAGACT GCAGAAGATT GGAGAAGGTT CATTTGGAAA AGCTGTTCTT 60

GTTAAATCGA CAGAGGATGG CAGACATTAT GTCATCAAGG AAATTAACAT CTCAAGAATG 120

TCTGATAAAG AAAGGCAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCAAA CATGAAGCAT 180

CCAAATATTG TCCAATATAA AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 240

GATTACTGTG AAGGAGGTGA TTTGTTTAAA CGAATAAATG CTCAGAAAGG CGCTCTGTTT 300

CAAGAAGACC AGATTTTGGA CTGGTTTGTG CAGATATGTT TGGCTCTGAA GCATGTACAT 360

GATAGAAAAA TTCTTCACCG AGACATAAAG TCACAGAACA TATTTCTAAC CAAAGATGGG 420

ACAGTGCAGC TTGGAGATTT TGGAATTGCT CGAGTTCTTA ATAGTACTGT AGAGCTGGCT 480

CGAACTTGCA TAGGCACTCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAGCCTTAT 540

AACAATAAAA GTGACATTTG GGCTTTGGGC TGTGTCCTTT ATGAGTTGTG TACACTTAAA 600

CATGCATTTG AAGCTGGAAA CATGAAAAAC CTGGTACTGA AGATAATCTC CGGATCCTTT 660

CCTCCAGTGT CTCCACATTA CTCCTATGAT CTCCGCAGCT TGCTGTCTCA GTTATTTAAA 720

AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCTAAA 780

CGAATCGAAA AGTTTCTCTC CCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACACTT 840

TCAAAGTTTG GACCACAGCC TCTCCCAGGT AAAAGACCAG CATCAGGACA AGGTGTCAGT 900

TCTTTTGTCC CTGCTCAGAA AATCACAAAG CCTGCTGCTA AATACGGAGT GCCTTTAACA 960

TATAAGAAGT ATGGAGATAA AAAGTTACTT GAGAAAAAAC CACCCCCAAA ACATAAACAG 1020

GCCCATCAAA TTCCCGTGAA GAAAATGAAT TCTGGAGAAG AAAGGAAGAA AATGTCTGAG 1080

GAAGCAGCAA AAAAAAGAAG GTTGGAATTT ATTGAGAAAG AAAAGAAGCA AAAGGATCAG 1140

ATTAGGTTCC TGAAGGCTGA GCAGATGAAG CGGCAAGAGA AGCAGCGGTT GGAGAGGATA 1200

AATAGGGCCA GGGAACAAGG ATGGAGGAAT GTTTTAAGGG CTGGTGGAAG CGGTGAAGTA 1260

AAGGCTTCCT TTTTTGGCAT TGGAGGGGCT GTCTCTCCAT CACCGTGTTC TCCTCGAGGC 1320

CAGTATGAAC ATTACCATGC CATTTTTGAC CAAATGCAGC GGCTAAGAGC AGAAGATAAT 1380

GAAGCAAGAT GGAAGGGGGG AATCTATGGT CGATGGCTCC CAGAAAGGCA AAAAGGACAC 1440

TTAGCTGTAG AGAGAGCCAA CCAAGTGGAA GAATTCCTAC AGCGTAAACG AGAAGCTATG 1500

CAGAATAAAG CCCGAGCCGA AGGACACGTG GTTTATTTGG CAAGACTGAG GCAAATAAGA 1560

CTACAAAATT TTAATGAGCG CCAACAGATT AAAGCCAAAC TTCGTGGTGA GAATAAAGAA 1620

GCTGATGGTA CCAAAGGACA AGAAGCAACT GAAGAGACTG ACATGAGGCT CAAAAAGATG 1680

GAGTCACTTA AGGCGCAAAC AAATGCACGT GCTGCTGTAC TAAAAGAACA GCTGGAGCGA 1740

AAAAGAAAGG AAGCTTATGA AAGAGAAAAG AAAGTATGGG AAGAACATTT GGTGGCGAGG 1800

GTAAAAAGCT CAGATGTTCC TCTGCCTTTG GAACTTCTTG AAACAGGTGG TTCTCCATCA 1860

AAGCAGCAGG TGAAGCCTGT CATTTCTGTG ACTTCAGCTT TGAAAGAAGT GGGCCTGGAT 1920

GGAAGTTTAA CTGATACCCA GGAAGAAGAA ATGGAAAAGA GTAACAGTGC TATTTCAAGT 1980

AAGCGAGAAA TCCTGCGTAG GCTAAATGAA AATCTTAAAG CTCAAGAGGA TGAAAAGGAA 2040

AAGCAGCATC ACTCAGGTTC TTGTGAGACC GTTGGTCACA AAGATGAGAG AGAGTATGAG 2100

ACAGAAAATG CCATTTCCTC TGATCGCAAG AAGTGGGAGA TGGGAGGTCA GCTTGTGATT 2160

CCTCTCGATG CAGTGACACT GGATACATCC TTCTCTGCAA CCGAAAAACA TACTGTGGGA 2220

GAGGTTATTA AATTAGATTC TAATGGCTCT CCAAGAAAAG TCTGGGGGAA AAACCCTACA 2280

GATTCTGTGC TGAAGATACT TGGAGAAGCT GAATTACAGC TACAGACAGA ACTACTAGAA 2340

AACACATCTT TTAAAAGTGA GGTTTATGCT GAAGAGGAGA ACTACAAACC CTTACTTACT 2400

GAAGAAGAGA ATCTGCAGTG CATTTCAAAA GAAATAAATC CATCAGCTAC TGTTGATTCT 2460

ACTGAAACGA AAAGTCCAAA GTTTACTGAG GTGTCTCCAC AAATGTCAGA AGGAAATGTG 2520

GAAGAACCTG ATGATTTGGA AACAGAAGTT CTACAAGAGC CAAGTAGCAC ACACACAGAT 2580

GGGAGTTTGC CACCTGTTCT TAATGATGTG TGGACTAGAG AGAAGGAAGC AGCTAAGGAA 2640

ACTGAGTTGG AAGATAAGGT TGCTGTGCAG CAGAGTGAAG TTTGTGAAGA TAGAATTCCA 2700

GGGAACGTGG ACCAATCCTG TAAGGATCAG AGAGATCCTG CAGTAGACGA TTCTCCGCAG 2760

TCTGGCTGTG ATGTAGAGAA GTCAGTACAG CCAGAATCGA TTTTCCAGAA AGTGGTTCAT 2820

TCTAAGGACT TGAACTTAGT TCAGGCAGTT CATTGCTCAC CAGAAGAACC AATTCCAATT 2880

CGATCTCACT CTGATTCTCC ACCAAAAACT AAGAGCAAGA ATTCCTTACT GATTGGACTT 2940

TCAACTGGTC TGTTTGATGC AAACAATCCA AAGATGCTGA GGACCTGCTC ACTTCCAGAT 3000

CTTTCCAAGC TGTTCAGAAC CCTAATGGAC GTTCCCACTG TGGGGGACGT TCATCAAGAC 3060

AGTCTTGAAA TCGATGAGCT GGAAGATGAA CCAATTAAAG AAGGGCCTTC TGATTCCGAA 3120

GACACTGTAT TTGAAGAAAC TGACACAGAT TTACAAGAGC TTCAGGCCTC AATGGAGCAG 3180

CTGCTTAGGG AGCAACCAGG TGACGAATAC AGTGAGGAGG AAGAGTCTGT TTTAAAAAGC 3240

AGCGATGTGG AGCAGACAGC AAGAGGGACA GATGCCCCAG ACGAGGAGGA CAACCCCAGC 3300

AGCGAAAGCG CCCTGAACGA GGAATGGCAC TCAGATAATA GTGACGCTGA GACCACTAGT 3360

GAATGTGAAT ATGACAGTGT CTTTAACCAT TTAGAGGAAC TAAGACTTCA CTTGGAGCAA 3420

GAAATGGGCT TTGAAAAGTT CTTTGAGGTT TATGAGAAAG TAAAGGCTAT TCATGAGGAT 3480

GAAGATGAAA ATATTGAAAT TTGTTCAACA ATAGTTGAGA ATATTTTGGG CAATGAGCAC 3540

CAGCATCTCT ATGCCAAGAT TCTGCATTTA GTCATGGCAG ATGGAGCCTA TCAGGAAGAT 3600

AATGATGAAT AA 3612

Domain Profile

S: 1     yevlkkiGrGsfgevlkvrkksdkkllvlKeikleeldekerqklvkevsllkqlkHpni  60

         y +l+kiG+Gsfg++++v++++d++++v+Kei+++++++kerq++ +ev +l+++kHpni

Q: 4     YVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQESRREVAVLANMKHPNI  63

         8899********************************************************

S: 61    veyyesfeeneelyivmeyceggdLsqlikeqkekkklleEktvlkilvqlllalkklHe  120

         v+y+esfeen +lyivm+yceggdL ++i+ q  k+ l++E+++l+++vq++lalk++H+

Q: 64    VQYKESFEENGSLYIVMDYCEGGDLFKRINAQ--KGALFQEDQILDWFVQICLALKHVHD  121

         *******************************9..9*************************

S: 121   kkilHRDlkpaNifltkkevvklgDfGlaklleskielaktlvGtpyYlsPEvleekpYn  180

         +kilHRD+k++Nifltk+++v+lgDfG+a++l+s++ela+t++GtpyYlsPE++e+kpYn

Q: 122   RKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYN  181

         ************************************************************

S: 181   eksDiWslgCvlyelcaLkkpFeaknllelvlkivegklepvpekyseelkeliesllkv  240

         +ksDiW+lgCvlyelc+Lk++Fea n+++lvlki++g+++pv+ +ys +l++l+++l+k+

Q: 182   NKSDIWALGCVLYELCTLKHAFEAGNMKNLVLKIISGSFPPVSPHYSYDLRSLLSQLFKR  241

         ************************************************************

S: 241   dpekrpsveeilkkp  255

         +p++rpsv++il+k

Q: 242   NPRDRPSVNSILEKG  256

         ***********9986

Domain Sequence
(FASTA)

YVRLQKIGEG SFGKAVLVKS TEDGRHYVIK EINISRMSDK ERQESRREVA VLANMKHPNI 60

VQYKESFEEN GSLYIVMDYC EGGDLFKRIN AQKGALFQED QILDWFVQIC LALKHVHDRK 120

ILHRDIKSQN IFLTKDGTVQ LGDFGIARVL NSTVELARTC IGTPYYLSPE ICENKPYNNK 180

SDIWALGCVL YELCTLKHAF EAGNMKNLVL KIISGSFPPV SPHYSYDLRS LLSQLFKRNP 240

RDRPSVNSIL EKG 253

Keyword

ATP-binding; Cell cycle; Cell division; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0031965	; C:nuclear membrane
GO:0005730	; C:nucleolus
GO:0005634	; C:nucleus
GO:0000242	; C:pericentriolar material
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0051301	; P:cell division
GO:0042384	; P:cilium assembly
GO:0007067	; P:mitosis
GO:0006974	; P:response to DNA damage stimulus
GO:0010212	; P:response to ionizing radiation

KEGG

mmu:18004

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013