EPS-MUM-00028

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00028

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	188.9	3.5E-55	782	1028	247

Status

Reviewed

Ensembl Protein

ENSMUSP00000034175

UniProt Accession

Q8BXA7; Q148U6; Q8BX96;

Protein Name

PH domain leucine-rich repeat-containing protein phosphatase 2

Protein Synonyms/Alias

PH domain leucine-rich repeat-containing protein phosphatase-like; PHLPP-like;

Gene Name

Phlpp2

Gene Synonyms/Alias

Phlpp2; Phlppl;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000031732	ENSMUSP00000034175	ENSMUST00000034175
ENSMUSG00000031732	ENSMUSP00000136166	ENSMUST00000179721

Organism

Mus musculus

Functional Description

Protein phosphatase that mediates dephosphorylation of'Ser-473' of AKT1, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor (By similarity).

Protein Length

1320

Protein Sequence
(FASTA)

MKHNGSRTCL NRRSRFGSRE RDWLREDVKR GCVYLYGADT TTATTTTSSS SSSSSSSDLH 60

LVLCTVETPA SEICAGEGRE SLYLQLHGDL VRRLEPSERP LQIVYDYLSR LGFEDPVRIQ 120

EEATNPDLSC MIRFYGEKPC QMDHLDRILL SGIYNVRKGK TQLHKWAERL VVLCGTCLIV 180

SSVKDCQTGK MHILPLVGGK IEEVKRRQHS LAFSSAGAQA QTYHVSFETL AEYQRWQRQA 240

SKVVSQRMST VDLSCYSLEE VPEHLFYSQD ITYLNLRHNF MQLERPGGLD TLHKFSQLKG 300

LNLSHNKLGL FPVLLCEIST LTELSLSCNG FHDLPSQIGK LLNLQTLSLD GNGLTALPDE 360

LGNLRQLTSL GISFNDFRHI PEVLEKLTML DKVAMAGNRL EVLNLGALTR MSQVKHVDLR 420

MNHLKTVITE NMEGNKHITH MDLRDNQLTD LDLSSLCSLE QLHCERNQLR ELTLSGFSLR 480

TLYASWNRLT AVNVYPVPSL LTSLELSQNL LECVPDWACE AKKLEILDIS HNLLTEVPMR 540

ILSSLSLRKL MVGHNHIHVL PALVEHIPLE VLDIQHNTLS RLPDTLFSKA LNLRYLNASA 600

NSLESLPSAC AGEESLSVLQ LLYLTSNLLT DQCIPVLVGH PHLRVLHLAN NQLQTFPASK 660

LNKLEQLEEL NLSGNKLTAI PTTIANCKRL HTLVAHANNI SIFPEILQLP QIQFVDLSCN 720

DLTEILIPEA LPATLQDLDL TGNTNLVLEH KTLDMFSHIT ALKIDQKPLP ATDSAVTSTF 780

WSHGLAEMAG QRNKLCVSAL AMDNFAEGVG AVYGMFDGDR NEELPRLLQC TMADVLLEEV 840

QHSTNDTVFM TNTFLVSHRK LGMAGQKLGS SALLCYIRPD TADPTSSFSL TVANVGMCQA 900

VLCRGGKPVP LSKVFSLEHD PEEAQRVKDQ KAIITEDNKV NGVTCCTRLL GCTYLYPWIL 960

PKPHIASTPL TIQDELLILG NKALWEHLSY LEAVNAVRHV QDPLAAAKKL CTLAQSYGCQ 1020

DNVGAMVVYL NIGEEGCTCE MNGLTLPGPV GFASTAALKD TPKPTTPSSS SGIASEFSSE 1080

MSTSEVSSEV GSTASDEHNT VGLEASLLPR PERRCSLHPA SSAGVFQRQP SCATFSSNQS 1140

DNGLDSDDDQ PVEGVITNGS RVEVEVDIHC CRGREPESSP PLPKNSSNAC SEERARGAGF 1200

GIRRQNSVNS GILLPANRDK MELQKSPSTS CLYGKKLSNG SIVPLEDSLN LIEVATEAPK 1260

RKTGYFAAPT QLEPEDQFVV PRDLEEEVKE QMKQHQEGRP EPEPRGEERT EPLEEFDTAL 1320

Nucleotide Sequence
(FASTA)

ATGAAACACA ATGGGAGCAG AACTTGTTTG AATAGAAGAA GTAGGTTTGG TTCCCGAGAA 60

AGAGACTGGC TAAGAGAAGA TGTGAAGAGA GGCTGTGTTT ACCTTTATGG AGCAGACACG 120

ACCACTGCCA CTACAACCAC CTCCTCCTCC TCCTCCTCCT CCTCCTCTTC TGATTTACAT 180

CTTGTCCTTT GCACAGTAGA GACACCAGCG TCAGAAATAT GTGCTGGAGA GGGAAGAGAA 240

AGCCTCTATC TACAGCTTCA TGGAGATCTG GTCAGGAGAC TGGAGCCCTC TGAACGGCCT 300

CTCCAGATTG TTTACGATTA CTTATCCAGG CTGGGGTTTG AAGATCCCGT GCGCATACAG 360

GAGGAGGCTA CGAACCCTGA CCTCAGCTGT ATGATTCGAT TTTATGGTGA AAAACCATGC 420

CAGATGGATC ATCTGGATCG AATCCTACTG TCTGGCATCT ATAATGTACG CAAAGGAAAA 480

ACCCAGCTGC ACAAATGGGC TGAGCGCCTC GTTGTTCTCT GTGGTACCTG CCTTATTGTT 540

TCCTCAGTGA AGGATTGTCA AACTGGAAAG ATGCACATTT TGCCGCTGGT TGGGGGAAAG 600

ATAGAAGAAG TGAAGCGCCG GCAGCACTCC CTTGCTTTCA GCTCAGCTGG AGCCCAAGCT 660

CAGACCTATC ATGTCAGCTT TGAGACTTTG GCTGAGTACC AGAGATGGCA ACGGCAGGCG 720

TCTAAGGTGG TGTCGCAGCG GATGAGCACT GTGGACCTCT CATGCTACAG CCTTGAAGAG 780

GTTCCTGAGC ATCTCTTCTA CAGCCAAGAC ATCACCTACC TCAACCTGCG GCACAACTTC 840

ATGCAGCTAG AAAGACCAGG GGGCCTTGAC ACACTCCACA AATTTTCTCA ACTGAAGGGC 900

CTGAACTTGT CCCATAATAA ACTTGGACTG TTTCCTGTGC TGCTGTGTGA GATTTCTACC 960

CTGACTGAGC TCAGCCTTTC TTGTAATGGA TTTCATGACT TGCCAAGTCA GATTGGTAAA 1020

TTGCTAAATC TTCAGACCCT CAGCCTCGAC GGCAACGGCC TCACGGCTCT GCCAGATGAG 1080

TTGGGAAACC TGCGGCAGCT CACTTCTCTG GGAATTTCCT TCAACGATTT TCGTCACATC 1140

CCTGAAGTTT TGGAGAAACT TACCATGTTA GATAAAGTGG CTATGGCAGG AAACCGTTTG 1200

GAGGTCCTGA ACCTGGGGGC ACTGACCAGG ATGAGCCAGG TCAAGCACGT GGATCTGAGG 1260

ATGAACCACT TGAAAACGGT GATTACTGAA AATATGGAAG GAAATAAACA TATCACGCAC 1320

ATGGATTTGC GGGACAATCA GTTGACTGAT TTGGACCTTA GCTCCTTATG TAGCTTGGAG 1380

CAGCTGCACT GTGAGCGGAA CCAGCTGAGG GAGCTGACAC TCAGCGGCTT CTCCCTTCGA 1440

ACCCTCTACG CCAGTTGGAA CAGGCTGACG GCAGTGAATG TGTACCCAGT GCCCAGCCTG 1500

CTCACTTCTT TGGAGCTCTC CCAAAACTTG CTGGAGTGTG TCCCTGACTG GGCCTGTGAA 1560

GCAAAAAAGC TGGAAATACT AGACATAAGC CACAATCTTC TTACAGAGGT CCCCATGAGA 1620

ATTCTGAGTA GTCTGAGTCT TCGGAAACTG ATGGTAGGAC ACAATCACAT CCATGTCCTT 1680

CCTGCACTGG TGGAGCACAT TCCCCTCGAG GTCCTGGATA TCCAGCATAA CACTCTCTCC 1740

AGGCTGCCGG ACACGCTCTT CTCCAAGGCC TTAAATCTCA GGTACTTGAA TGCATCTGCA 1800

AATAGTCTGG AGTCCTTGCC TTCTGCGTGT GCTGGAGAGG AGAGTCTGAG TGTGCTGCAG 1860

CTGCTGTACC TGACCAGCAA CCTTCTGACA GATCAGTGCA TACCAGTTTT GGTCGGGCAC 1920

CCACACCTGC GAGTTCTGCA CCTTGCAAAC AACCAGCTCC AGACCTTCCC TGCAAGCAAG 1980

CTAAATAAAC TGGAGCAGCT GGAAGAGCTG AATCTAAGTG GGAACAAACT TACAGCTATC 2040

CCCACAACCA TAGCCAACTG CAAAAGGCTG CACACCCTTG TCGCCCATGC CAACAACATC 2100

AGCATCTTCC CAGAGATCCT GCAGCTGCCT CAGATTCAGT TTGTAGACCT GAGTTGCAAT 2160

GACTTGACAG AAATCCTAAT TCCAGAAGCT CTGCCTGCTA CTTTACAAGA CCTTGATCTG 2220

ACAGGAAATA CAAATCTGGT CCTGGAACAT AAGACTTTGG ACATGTTTAG CCACATCACA 2280

GCCCTGAAGA TTGATCAGAA GCCTTTGCCA GCCACAGACT CTGCAGTTAC ATCAACCTTC 2340

TGGAGCCATG GCCTGGCCGA GATGGCAGGG CAGAGGAATA AACTCTGTGT GTCTGCCCTG 2400

GCTATGGATA ACTTTGCAGA GGGTGTGGGA GCTGTCTACG GCATGTTTGA TGGGGACCGG 2460

AATGAAGAAC TCCCTCGTCT GCTGCAGTGT ACAATGGCAG ATGTGCTTTT GGAAGAAGTA 2520

CAGCACTCAA CAAATGACAC AGTTTTCATG ACCAATACCT TCTTGGTATC TCACAGGAAA 2580

TTAGGAATGG CTGGCCAGAA GCTGGGTTCT TCGGCTCTCC TGTGCTATAT TCGACCTGAC 2640

ACTGCCGACC CAACAAGTAG CTTTAGCTTG ACTGTAGCCA ATGTTGGCAT GTGCCAAGCT 2700

GTCCTGTGCC GAGGTGGGAA GCCAGTACCT CTTTCTAAAG TCTTCAGCTT GGAGCATGAC 2760

CCAGAGGAGG CTCAGAGGGT GAAAGACCAG AAAGCCATCA TAACAGAGGA CAACAAGGTG 2820

AACGGAGTAA CCTGCTGCAC CCGGCTGCTG GGGTGCACAT ACCTCTACCC ATGGATCCTC 2880

CCCAAGCCCC ACATAGCTTC CACTCCACTT ACCATTCAAG ATGAATTGCT GATTTTGGGA 2940

AACAAAGCAC TGTGGGAACA CTTGTCATAT CTAGAAGCTG TCAATGCAGT ACGTCACGTG 3000

CAAGACCCAC TAGCTGCTGC TAAGAAGCTG TGCACATTAG CCCAGAGCTA CGGTTGTCAG 3060

GACAACGTGG GGGCAATGGT GGTTTATTTG AATATTGGTG AAGAAGGCTG TACCTGTGAA 3120

ATGAATGGGC TCACCCTTCC AGGCCCTGTG GGATTTGCTT CAACTGCAGC CCTCAAGGAT 3180

ACTCCCAAGC CAACCACTCC CTCCTCCAGT AGTGGGATTG CCTCCGAGTT CAGCAGTGAG 3240

ATGTCCACCT CAGAGGTGAG CAGTGAAGTG GGCTCCACGG CCTCGGATGA GCACAACACT 3300

GTGGGCCTGG AGGCGAGCTT GCTGCCCAGG CCAGAGAGGC GCTGCAGTCT GCACCCAGCG 3360

TCCTCCGCAG GGGTGTTCCA GCGCCAGCCT TCTTGTGCTA CTTTTTCCAG CAATCAGTCT 3420

GACAATGGCC TGGACAGTGA CGATGACCAG CCTGTTGAAG GAGTCATAAC CAACGGCAGC 3480

AGGGTTGAAG TAGAGGTAGA CATCCATTGC TGCCGAGGCA GAGAGCCAGA GAGCTCCCCT 3540

CCGCTCCCGA AGAACTCTTC CAACGCCTGT TCAGAGGAGC GTGCTAGAGG GGCGGGGTTT 3600

GGGATCCGAA GACAGAACAG TGTCAACAGT GGTATACTTC TGCCAGCGAA CAGAGACAAG 3660

ATGGAGCTGC AGAAGTCTCC CTCCACTTCT TGTCTGTATG GAAAGAAACT CTCCAATGGC 3720

TCCATTGTGC CCCTAGAGGA CAGCCTGAAC CTCATTGAGG TGGCCACAGA AGCACCCAAA 3780

AGGAAAACTG GCTACTTTGC TGCCCCCACT CAGCTGGAAC CAGAGGATCA GTTTGTCGTC 3840

CCTCGAGACC TGGAAGAGGA AGTGAAGGAG CAGATGAAGC AGCACCAGGA GGGCAGGCCT 3900

GAGCCCGAGC CCCGGGGAGA GGAGCGGACA GAGCCCCTGG AGGAGTTTGA TACAGCACTG 3960

TGA 3963

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         ++g++++ g+rn+++ ++ +++nf++  +     a++g++DG++++e  + +++++ ++l

Q: 782   SHGLAEMAGQRNKLCVSALAMDNFAEGVG-----AVYGMFDGDRNEELPRLLQCTMADVL  836

         6899*********************9877.....59************************

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidksGstavvalikds......  115

          ee++    ++++ + +++++Fl  +++l       ++ +k Gs+a++++i+++

Q: 837   LEEVQ----HSTNDTVFMTNTFLVSHRKL------GMAGQKLGSSALLCYIRPDtadpts  886

         ***99....88888889************......89***************999999**

S: 116   ..klyvanvGdSravLcrngkavelssdHkpsre.dEleRieeaggsvilkdgaRvlGvL  172

           +l+vanvG ++avLcr+gk+v+ls+ ++ +++ +E +R++++++ +++++  +v+Gv+

Q: 887   sfSLTVANVGMCQAVLCRGGKPVPLSKVFSLEHDpEEAQRVKDQKAIITEDN--KVNGVT  944

         *******************************9876**********9999998..******

S: 173   avsRalGdlylkpyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvlekk  232

         +++R lG++yl+p++ p+P++ s++lt +de liL+ + lw+++s  eav++vr+v +

Q: 945   CCTRLLGCTYLYPWILPKPHIASTPLTIQDELLILGNKALWEHLSYLEAVNAVRHVQD--  1002

         **********************************************************..

S: 233   eaeqeaakkLvklalargskdnvsaivv  260

           + +aakkL++la+++g++dnv a+vv

Q: 1003  --PLAAAKKLCTLAQSYGCQDNVGAMVV  1028

         ..************************97

Domain Sequence
(FASTA)

SHGLAEMAGQ RNKLCVSALA MDNFAEGVGA VYGMFDGDRN EELPRLLQCT MADVLLEEVQ 60

HSTNDTVFMT NTFLVSHRKL GMAGQKLGSS ALLCYIRPDT ADPTSSFSLT VANVGMCQAV 120

LCRGGKPVPL SKVFSLEHDP EEAQRVKDQK AIITEDNKVN GVTCCTRLLG CTYLYPWILP 180

KPHIASTPLT IQDELLILGN KALWEHLSYL EAVNAVRHVQ DPLAAAKKLC TLAQSYGCQD 240

NVGAMVV 247

Keyword

Complete proteome; Cytoplasm; Hydrolase; Leucine-rich repeat; Manganese; Membrane; Metal-binding; Nucleus; Protein phosphatase; Reference proteome; Repeat; Tumor suppressor.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0016020	; C:membrane
GO:0005634	; C:nucleus
GO:0046872	; F:metal ion binding
GO:0004721	; F:phosphoprotein phosphatase activity
GO:0008152	; P:metabolic process

KEGG

mmu:244650

;

InterPros

IPR001611	; Leu-rich_rpt.
IPR025875	; Leu-rich_rpt_4.
IPR003591	; Leu-rich_rpt_typical-subtyp.
IPR011993	; PH_like_dom.
IPR001932	; PP2C-like.

Pfam

PF00560	; LRR_1; 1.
PF12799	; LRR_4; 1.
PF00481	; PP2C; 1.

SMARTs

SM00369	; LRR_TYP; 1.
SM00332	; PP2Cc; 1.

Prosites

PS51450	; LRR; 17.
PS50003	; PH_DOMAIN; FALSE_NEG.

Prints

Created Date

20-Feb-2013