EKS-MUM-00033

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00033

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TK/Jak	336.4	3.4E-100	818	1091	274

Status

Reviewed

Ensembl Protein

ENSMUSP00000060073

UniProt Accession

Q62137; A2RRI3; Q0D2M8; Q61746; Q61747; Q8BTY6; Q8BYU2;

Protein Name

Tyrosine-protein kinase JAK3

Protein Synonyms/Alias

Janus kinase 3; JAK-3;

Gene Name

Jak3

Gene Synonyms/Alias

Jak3;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000031805	ENSMUSP00000105639	ENSMUST00000110012
ENSMUSG00000031805	ENSMUSP00000060073	ENSMUST00000051995
ENSMUSG00000031805	ENSMUSP00000105640	ENSMUST00000110013

Organism

Mus musculus

Functional Description

Non-receptor tyrosine kinase involved in variousprocesses such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Protein Length

1100

Protein Sequence
(FASTA)

MAPPSEETPL IPQRSCSLSS SEAGALHVLL PPRGPGPPQR LSFSFGDYLA EDLCVRAAKA 60

CGILPVYHSL FALATEDFSC WFPPSHIFCI EDVDTQVLVY RLRFYFPDWF GLETCHRFGL 120

RKDLTSAILD LHVLEHLFAQ HRSDLVSGRL PVGLSMKEQG EFLSLAVLDL AQMAREQAQR 180

PGELLKTVSY KACLPPSLRD VIQGQNFVTR RRIRRTVVLA LRRVVACQAD RYALMAKYIL 240

DLERLHPAAT TETFRVGLPG AQEEPGLLRV AGDNGISWSS GDQELFQTFC DFPEIVDVSI 300

KQAPRVGPAG EHRLVTVTRM DGHILEAEFP GLPEALSFVA LVDGYFRLIC DSRHYFCKEV 360

APPRLLEEEA ELCHGPITLD FAIHKLKAAG SLPGTYILRR SPQDYDSFLL TACVQTPLGP 420

DYKGCLIRQD PSGAFSLVGL SQPHRSLREL LAACWNSGLR VDGAALNLTS CCAPRPKEKS 480

NLIVVRRGCT PAPAPGCSPS CCALTQLSFH TIPTDSLEWH ENLGHGSFTK IFRGRRREVV 540

DGETHDSEVL LKVMDSRHRN CMESFLEAAS LMSQVSYPHL VLLHGVCMAG DSIMVQEFVY 600

LGAIDMYLRK RGHLVSASWK LQVTKQLAYA LNYLEDKGLP HGNVSARKVL LAREGGDGNP 660

PFIKLSDPGV SPTVLSLEML TDRIPWVAPE CLQEAQTLGL EADKWGFGAT TWEVFSGGPA 720

HITSLEPAKK LKFYEDQGQL PALKWTELAG LITQCMAYDP GRRPSFRAIL RDLNGLITSD 780

YELLSDPTPG IPSPRDELCG GAQLYACQDP AIFEERHLKY ISLLGKGNFG SVELCRYDPL 840

GDNTGPLVAV KQLQHSGPDQ QRDFQREIQI LKALHSDFIV KYRGVSYGPG RQSLRLVMEY 900

LPSGCLRDFL QRHRARLHTD RLLLFAWQIC KGMEYLGARR CVHRDLAARN ILVESEAHVK 960

IADFGLAKLL PLGKDYYVVR EPGQSPIFWY APESLSDNIF SRQSDVWSFG VVLYELFTYC 1020

DKSCSPSAEF LSMMGPEREG PPLCRLLELL AEGRRLPPPP TCPTEVQELM QLCWAPSPHD 1080

RPAFGTLSPQ LDALWRGRPG 1100

Nucleotide Sequence
(FASTA)

ATGGCACCTC CAAGTGAGGA GACACCTCTG ATCCCTCAGC GCTCTTGCAG CCTCTCATCC 60

TCAGAGGCAG GAGCCCTGCA TGTGCTCCTT CCTCCCCGGG GACCTGGGCC TCCCCAGCGA 120

TTGTCATTCT CTTTTGGGGA CTACTTGGCT GAGGATTTAT GTGTGCGAGC TGCCAAGGCC 180

TGTGGCATCC TGCCTGTTTA TCATTCGCTT TTCGCTCTGG CCACTGAGGA CTTCTCTTGC 240

TGGTTTCCCC CAAGCCACAT CTTCTGCATA GAGGACGTGG ACACTCAAGT CTTGGTCTAC 300

AGGCTACGCT TTTATTTCCC TGACTGGTTT GGGCTGGAGA CATGTCACCG CTTTGGGCTG 360

CGCAAAGATT TGACCAGTGC CATCCTTGAC TTACACGTTT TAGAACATCT CTTTGCTCAG 420

CACCGCAGTG ACCTGGTGAG TGGGCGCCTC CCGGTGGGCC TTAGCATGAA GGAGCAGGGA 480

GAGTTCCTGA GCCTGGCCGT GCTGGACTTG GCCCAGATGG CTCGTGAGCA GGCCCAGCGC 540

CCAGGAGAGC TGCTGAAGAC GGTCAGTTAC AAAGCCTGTC TGCCGCCCAG CCTGCGCGAT 600

GTGATCCAGG GCCAGAACTT CGTGACACGC AGGCGCATCC GCAGGACCGT GGTCTTGGCG 660

CTGCGCCGTG TGGTCGCCTG CCAGGCCGAC CGCTACGCGC TCATGGCCAA GTATATTTTG 720

GACCTGGAGC GGCTACATCC AGCGGCCACC ACCGAGACCT TCCGTGTGGG GCTCCCGGGC 780

GCCCAGGAGG AGCCGGGGCT TCTGCGTGTG GCGGGGGACA ACGGCATCTC CTGGAGCTCC 840

GGGGACCAGG AGCTTTTCCA GACCTTCTGT GACTTTCCGG AAATCGTGGA TGTCAGCATC 900

AAGCAGGCCC CACGTGTGGG TCCGGCAGGG GAACACCGGC TGGTCACCGT CACCAGGATG 960

GACGGCCACA TCCTGGAAGC GGAGTTTCCG GGGCTGCCTG AGGCGCTGTC CTTCGTGGCC 1020

CTTGTGGATG GGTACTTCCG CCTGATCTGC GACTCCAGGC ATTATTTCTG CAAGGAGGTG 1080

GCGCCGCCGC GGCTGCTGGA GGAGGAGGCG GAGCTGTGCC ATGGACCCAT CACGTTAGAC 1140

TTTGCCATCC ACAAGCTGAA GGCCGCTGGC TCCCTCCCAG GCACCTACAT TCTCCGCCGC 1200

AGCCCGCAGG ACTATGACAG CTTTCTTCTT ACCGCCTGCG TCCAGACTCC TCTTGGCCCC 1260

GACTACAAGG GCTGCCTCAT CCGCCAGGAC CCCAGCGGGG CTTTCTCCCT GGTTGGCCTC 1320

AGCCAGCCCC ACAGAAGCCT GCGGGAGCTG CTTGCAGCCT GCTGGAATTC TGGGCTGCGA 1380

GTAGACGGTG CTGCCCTGAA CCTAACATCC TGCTGCGCTC CCAGACCCAA GGAAAAGTCC 1440

AATTTGATCG TGGTGCGAAG GGGCTGCACC CCCGCGCCTG CCCCTGGCTG CTCCCCGTCC 1500

TGCTGTGCGC TGACACAGCT GAGCTTCCAC ACAATTCCAA CGGACAGCCT GGAGTGGCAC 1560

GAGAACCTGG GTCACGGTTC TTTTACCAAG ATCTTCCGTG GCCGCAGGCG GGAGGTCGTG 1620

GATGGTGAGA CACATGACTC GGAAGTCCTC CTGAAGGTCA TGGACTCCAG ACATCGGAAC 1680

TGCATGGAGT CTTTTCTGGA AGCCGCAAGC TTGATGAGCC AAGTATCCTA CCCGCACCTG 1740

GTGTTACTGC ACGGCGTCTG CATGGCTGGA GACAGCATCA TGGTGCAGGA ATTTGTGTAT 1800

CTAGGAGCAA TTGACATGTA CCTGCGCAAG CGTGGCCACC TGGTGTCAGC CAGCTGGAAA 1860

CTGCAGGTGA CCAAGCAGCT GGCATATGCC CTTAACTACT TGGAGGACAA AGGCCTTCCT 1920

CACGGCAACG TCTCAGCACG GAAGGTGCTC CTGGCTCGTG AGGGGGGTGA TGGGAATCCA 1980

CCTTTCATTA AGCTGAGTGA TCCTGGTGTC AGTCCCACTG TGCTGAGCCT GGAAATGCTC 2040

ACCGACAGAA TACCCTGGGT GGCCCCCGAA TGTCTCCAGG AGGCTCAGAC ACTCGGCTTG 2100

GAGGCTGACA AGTGGGGCTT TGGAGCCACC ACGTGGGAGG TGTTCAGCGG GGGACCCGCC 2160

CACATCACCT CGCTGGAGCC CGCCAAAAAG CTGAAGTTCT ATGAGGACCA GGGACAGCTG 2220

CCCGCTCTCA AATGGACAGA ACTGGCGGGA CTTATCACAC AGTGCATGGC CTATGATCCT 2280

GGCCGGCGCC CCTCCTTCCG AGCTATCCTC AGAGACCTCA ACGGCCTCAT TACATCAGAT 2340

TACGAGCTCC TCTCAGACCC CACACCTGGC ATCCCGAGTC CTCGAGATGA GCTGTGCGGT 2400

GGCGCCCAGC TCTATGCCTG CCAGGACCCC GCCATATTCG AGGAGAGACA CCTTAAGTAC 2460

ATCTCTTTGC TGGGCAAGGG CAACTTTGGC AGCGTGGAGC TGTGCCGCTA TGACCCCCTG 2520

GGGGACAATA CGGGACCCCT GGTGGCAGTG AAACAGCTAC AGCACAGCGG GCCAGACCAG 2580

CAGAGGGACT TCCAGCGGGA GATTCAGATC CTTAAGGCTC TGCACAGCGA CTTCATCGTC 2640

AAGTACCGGG GAGTCAGCTA TGGGCCAGGT CGCCAGAGCC TGCGGTTGGT GATGGAGTAC 2700

CTGCCCAGCG GCTGCCTGCG AGACTTCCTG CAGCGCCATC GCGCGCGCCT GCACACCGAC 2760

CGCCTACTGC TGTTCGCTTG GCAGATCTGC AAGGGCATGG AGTACCTGGG TGCGCGCCGC 2820

TGCGTACACC GTGACCTGGC TGCGCGCAAC ATCTTGGTGG AGAGCGAGGC TCATGTGAAG 2880

ATCGCGGACT TCGGCCTCGC TAAGCTGCTG CCCCTGGGAA AGGACTACTA CGTGGTCCGC 2940

GAGCCTGGCC AAAGCCCCAT CTTTTGGTAT GCCCCGGAGT CCCTATCTGA CAACATCTTC 3000

TCCCGCCAAT CTGACGTGTG GAGCTTCGGA GTGGTGTTGT ACGAGCTCTT CACCTACTGC 3060

GACAAGAGCT GCAGCCCATC CGCTGAGTTC CTGAGCATGA TGGGGCCTGA GCGTGAAGGA 3120

CCCCCGCTCT GCCGCCTCCT GGAGCTGCTG GCAGAGGGCC GACGCCTCCC ACCACCTCCC 3180

ACCTGCCCCA CCGAGGTTCA GGAGCTCATG CAGCTGTGCT GGGCGCCCAG CCCGCACGAC 3240

CGGCCAGCCT TCGGCACCCT GAGCCCCCAG CTGGACGCGC TGTGGCGTGG AAGACCCGGA 3300

TAG 3303

Domain Profile

S: 1     lkqiesLgkgtftkvfevredaegdsteeevalkvLkesgedisrdfkreaeilsqlshe  60

         lk+i+ Lgkg+f++v+++r+d+ gd+t+  va+k+L++sg+d++rdf+re++il++l+++

Q: 818   LKYISLLGKGNFGSVELCRYDPLGDNTGPLVAVKQLQHSGPDQQRDFQREIQILKALHSD  877

         799*********************************************************

S: 61    hivklkgvcvaegen..rlVmeyvesGsLdiyLrkkkealslsslldvakqlakameYLe  118

         +ivk++gv++ +g++  rlVmey++sG+L+++L++++++l++ +ll +a+q++k+meYL+

Q: 878   FIVKYRGVSYGPGRQslRLVMEYLPSGCLRDFLQRHRARLHTDRLLLFAWQICKGMEYLG  937

         ************************************************************

S: 119   dkklvhrdvaarnvLveresnegtppfvklsdpglakavnqd.eYylvreerdsripwla  177

         ++++vhrd+aarn+Lve+e++      vk++d+glak+++ + +Yy+vre+++s+i+w+a

Q: 938   ARRCVHRDLAARNILVESEAH------VKIADFGLAKLLPLGkDYYVVREPGQSPIFWYA  991

         *********************......*********************************

S: 178   peclseaknlstasdvWsFgvtlyellt.cskgesp....lrsesle.......rklell  225

         pe+ls++  +s++sdvWsFgv+lyel+t c+k++sp    l+++++e       r+lell

Q: 992   PESLSDNI-FSRQSDVWSFGVVLYELFTyCDKSCSPsaefLSMMGPEregpplcRLLELL  1050

         ********.***************************************************

S: 226   eekerlpkpk.cpaelaelmtnclsyepsqrpsFrailedl  265

          e++rlp p+ cp+e++elm+ c++ +p++rp+F ++  +l

Q: 1051  AEGRRLPPPPtCPTEVQELMQLCWAPSPHDRPAFGTLSPQL  1091

         ***********************************998876

Domain Sequence
(FASTA)

LKYISLLGKG NFGSVELCRY DPLGDNTGPL VAVKQLQHSG PDQQRDFQRE IQILKALHSD 60

FIVKYRGVSY GPGRQSLRLV MEYLPSGCLR DFLQRHRARL HTDRLLLFAW QICKGMEYLG 120

ARRCVHRDLA ARNILVESEA HVKIADFGLA KLLPLGKDYY VVREPGQSPI FWYAPESLSD 180

NIFSRQSDVW SFGVVLYELF TYCDKSCSPS AEFLSMMGPE REGPPLCRLL ELLAEGRRLP 240

PPPTCPTEVQ ELMQLCWAPS PHDRPAFGTL SPQL 274

Keyword

Adaptive immunity; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Immunity; Innate immunity; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; SH2 domain; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Felis catus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ornithorhynchus anatinus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>

Gene Ontology

GO:0005856	; C:cytoskeleton
GO:0005829	; C:cytosol
GO:0012505	; C:endomembrane system
GO:0016020	; C:membrane
GO:0005524	; F:ATP binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0030183	; P:B cell differentiation
GO:0019221	; P:cytokine-mediated signaling pathway
GO:0007204	; P:elevation of cytosolic calcium ion concentration
GO:0007167	; P:enzyme linked receptor protein signaling pathway
GO:0045087	; P:innate immune response
GO:0035771	; P:interleukin-4-mediated signaling pathway
GO:0048535	; P:lymph node development
GO:0002731	; P:negative regulation of dendritic cell cytokine production
GO:0045221	; P:negative regulation of FasL biosynthetic process
GO:0032693	; P:negative regulation of interleukin-10 production
GO:0032695	; P:negative regulation of interleukin-12 production
GO:0045626	; P:negative regulation of T-helper 1 cell differentiation
GO:0070244	; P:negative regulation of thymocyte apoptotic process
GO:0042104	; P:positive regulation of activated T cell proliferation
GO:0051928	; P:positive regulation of calcium ion transport
GO:0050778	; P:positive regulation of immune response
GO:0045944	; P:positive regulation of transcription from RNA polymerase II promoter
GO:0046777	; P:protein autophosphorylation
GO:0070672	; P:response to interleukin-15
GO:0070669	; P:response to interleukin-2
GO:0070670	; P:response to interleukin-4
GO:0071104	; P:response to interleukin-9
GO:0043029	; P:T cell homeostasis
GO:0042506	; P:tyrosine phosphorylation of Stat5 protein

KEGG

mmu:16453

;

InterPros

IPR019749	; Band_41_domain.
IPR000299	; FERM_domain.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR000980	; SH2.
IPR008266	; Tyr_kinase_AS.
IPR020635	; Tyr_kinase_cat_dom.
IPR016251	; Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020775	; Tyr_kinase_non-rcpt_Jak3.

Pfam

PF07714

; Pkinase_Tyr; 2.

SMARTs

SM00295	; B41; 1.
SM00252	; SH2; 1.
SM00219	; TyrKc; 2.

Prosites

PS00660	; FERM_1; FALSE_NEG.
PS00661	; FERM_2; FALSE_NEG.
PS50057	; FERM_3; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 2.
PS00109	; PROTEIN_KINASE_TYR; 1.
PS50001	; SH2; FALSE_NEG.

Prints

PR01823	; JANUSKINASE.
PR01826	; JANUSKINASE3.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013