EKS-MUM-00410

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00410

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	345.6	4.2E-103	111	382	272

Status

Reviewed

Ensembl Protein

ENSMUSP00000034316

UniProt Accession

Q8VDG6; Q5DTU6; Q811F5;

Protein Name

Mitogen-activated protein kinase kinase kinase MLK4

Protein Synonyms/Alias

Mixed lineage kinase 4;

Gene Name

Mlk4

Gene Synonyms/Alias

Mlk4; Kiaa1804;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000031853	ENSMUSP00000034316	ENSMUST00000034316

Organism

Mus musculus

Functional Description

Negative regulator of TLR4 signaling. Does not activateJNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways (By similarity).

Protein Length

1002

Protein Sequence
(FASTA)

MALPVAEGTA DTPLSPARDD SGSTSSGMWA ALYDYEARGE DELSLRRGQL VEVLSQDAAV 60

SGDEGWWAGQ VQRRLGIFPA SYVAPCGPVP PPAPPPPRPC SPVHVDFERL ELKELIGAGG 120

FGQVYRATWQ GQEVAVKAAR RDPEQDAAAA AESVRREARL FAMLRHPNII QLRGVCLRQP 180

HLCLVLEFAR GGALNRALAA AASDPRAPGP RRARRIPPQV LVNWAVQIAR GMLYLHEEAV 240

VPILHRDLKS SNILLLEKIE HDDICNKTLK ITDFGLAREW HRTTRMSAAG TYAWMAPEVI 300

RSSLFSKGSD IWSYGVLLWE LLTGEVPYRG IDGLAVAYGV AVNKLTLPIP STCPEPFAKL 360

MKECWEQDPH IRPSFALILQ QLTAIEEAVL TNMPQESFHS MQEDWKLEIQ QMFSELRTKE 420

KELRSREEEL SRAALQQKSQ ELLLRRREQQ LAEREIDVLE RELNVLIFQL SQEAPHVKKR 480

KGRFRRGRLR LKDGHRISLP SDFQHKITVQ ASPTLDKRRS SDSGLCSPPG SPLMLPRLRA 540

IQLTSDENNK TRGRNMVFRQ EDFEDVKRSF KKKGCTWGPS SVQTKERPEG RERVRPLSDG 600

NSPWSSLLIK SQKTTPLASL FVDQPGSCEE QKLVPEGLEH RKPKQTKFPG QAHVGLPLCK 660

DSQREDSSEA ESREEGSPKG SPVNNVGAPM LRKKTESALC ECGMLLASMA LGLDVRKLHG 720

AQAPAKPSPK MEKKEEGALQ PASRCQSSPS SLLRQPSAGR APSGGSTLLL PSAPSHSSKS 780

SLSMKCLLQA GKEESSLGNA RDLCGPTTLT PDPGSAAPES GCELIPGLRP KTDYGVLRSM 840

PHAILEQTGE RLPGCAIVGD KGCHHMQMGS EETPLWLQSA PEDSGLPHSP SPGPQRDLAS 900

QASLVKPEGV LGECQACPAL PQRPHTASVR TTSPPTWVCD KDHQVPALAC LLGAQERSRC 960

QTPSLLDASI EGQKKDCAMP LCRVKSVMCQ PSIYALEKDF LT 1002

Nucleotide Sequence
(FASTA)

ATGGCTCTAC CTGTAGCCGA GGGCACGGCT GACACGCCGC TGTCCCCGGC CCGGGACGAC 60

TCGGGGAGTA CCTCCTCCGG GATGTGGGCC GCGCTTTATG ATTACGAAGC GCGCGGTGAG 120

GACGAGCTGA GCCTGAGGCG CGGTCAGCTG GTCGAGGTAC TGTCGCAGGA CGCCGCGGTG 180

TCAGGAGACG AGGGCTGGTG GGCCGGCCAA GTGCAGCGGC GCCTGGGCAT CTTTCCGGCC 240

AGCTACGTGG CGCCCTGTGG CCCGGTGCCG CCGCCGGCGC CGCCCCCGCC GAGACCCTGC 300

TCGCCTGTGC ACGTAGACTT CGAGCGCCTG GAGCTCAAGG AGCTGATCGG CGCCGGCGGC 360

TTCGGTCAGG TGTACCGCGC CACCTGGCAG GGCCAGGAGG TGGCGGTGAA GGCGGCGCGC 420

CGGGACCCGG AGCAGGACGC GGCGGCGGCG GCCGAGAGCG TACGGCGCGA GGCGCGGCTC 480

TTCGCTATGC TGCGGCACCC CAACATCATC CAGCTGCGGG GTGTGTGCCT GCGGCAGCCG 540

CACCTCTGCC TGGTGCTGGA GTTCGCGCGT GGCGGCGCGC TCAACCGCGC GCTGGCGGCG 600

GCAGCATCGG ATCCGCGAGC GCCCGGCCCC CGCCGCGCGC GCCGCATCCC CCCACAGGTT 660

CTGGTCAACT GGGCGGTGCA GATCGCCCGC GGCATGCTGT ACCTGCACGA GGAGGCGGTG 720

GTGCCCATCC TGCACCGTGA CCTCAAGTCC AGCAACATTT TGCTGCTGGA GAAGATAGAA 780

CATGATGACA TTTGCAACAA GACCCTGAAG ATCACGGACT TTGGGCTAGC GAGGGAATGG 840

CACAGGACCA CCAGGATGAG CGCAGCAGGG ACCTACGCCT GGATGGCTCC CGAGGTCATC 900

AGGTCCTCCC TGTTCTCCAA GGGCAGCGAC ATCTGGAGCT ATGGAGTGCT GTTGTGGGAG 960

CTGCTCACTG GTGAGGTCCC GTACCGTGGC ATCGATGGAC TGGCTGTGGC GTACGGGGTG 1020

GCTGTCAACA AGCTCACACT GCCTATTCCA TCCACCTGCC CTGAACCGTT TGCAAAACTC 1080

ATGAAAGAGT GCTGGGAACA AGACCCTCAT ATCCGCCCCT CGTTTGCCTT AATTCTTCAG 1140

CAGCTGACTG CGATCGAAGA GGCAGTGTTG ACCAACATGC CCCAGGAGTC CTTCCATTCC 1200

ATGCAGGAAG ACTGGAAACT GGAGATTCAG CAAATGTTCA GTGAGCTGCG AACCAAGGAG 1260

AAGGAGCTGC GTTCCCGAGA GGAGGAGCTG AGCCGAGCTG CCTTGCAACA GAAATCGCAG 1320

GAACTGCTGC TCAGACGCCG CGAGCAGCAG CTCGCGGAGC GTGAGATCGA CGTGCTGGAG 1380

CGCGAGCTGA ACGTGCTCAT CTTCCAGCTG AGCCAGGAGG CTCCCCACGT GAAGAAGAGG 1440

AAGGGAAGGT TCAGGAGAGG ACGGCTGAGG CTCAAAGACG GCCACCGCAT CAGCCTGCCT 1500

TCAGATTTCC AGCACAAGAT CACAGTGCAG GCTTCCCCGA CGCTGGACAA GCGCCGAAGC 1560

TCAGACAGCG GACTGTGCAG CCCACCCGGC AGCCCCTTGA TGCTCCCCCG CCTCCGGGCT 1620

ATTCAGTTAA CTTCAGATGA GAACAATAAA ACCCGGGGAA GGAACATGGT CTTCCGCCAA 1680

GAAGACTTTG AAGACGTGAA AAGGAGTTTC AAGAAAAAAG GATGTACGTG GGGACCAAGC 1740

TCCGTTCAAA CAAAGGAAAG ACCAGAAGGC AGAGAGAGGG TCAGACCTCT CTCAGATGGC 1800

AACAGCCCTT GGTCATCTCT TTTAATAAAA AGCCAGAAAA CCACACCCTT GGCTTCACTG 1860

TTTGTGGACC AGCCAGGTTC TTGTGAGGAG CAGAAACTTG TCCCTGAAGG ATTAGAACAT 1920

AGAAAACCAA AGCAAACGAA GTTCCCTGGC CAGGCCCACG TTGGTCTACC TCTTTGCAAA 1980

GACAGCCAGA GAGAAGACTC ATCAGAGGCT GAGAGTCGGG AGGAGGGATC CCCTAAGGGG 2040

TCCCCGGTGA ACAACGTGGG TGCACCCATG CTGAGGAAGA AAACGGAGTC AGCGCTGTGT 2100

GAGTGTGGGA TGCTGCTGGC ATCGATGGCT TTGGGACTGG ATGTCAGGAA ACTTCACGGA 2160

GCCCAGGCCC CCGCAAAACC ATCACCCAAG ATGGAAAAGA AGGAAGAGGG TGCCCTCCAA 2220

CCTGCCTCCA GGTGCCAATC AAGTCCCAGT TCCCTTCTGA GGCAGCCATC TGCAGGCAGG 2280

GCACCCAGCG GTGGCTCCAC GCTCCTGCTG CCCAGTGCCC CAAGCCATTC CTCAAAGTCT 2340

TCTCTCTCCA TGAAGTGTTT GCTGCAGGCT GGTAAGGAGG AGTCATCTTT GGGGAATGCT 2400

CGGGACTTGT GTGGTCCTAC GACGCTCACT CCAGATCCTG GCTCTGCCGC ACCAGAGAGC 2460

GGCTGTGAAC TCATCCCCGG ACTGAGACCT AAGACTGACT ATGGTGTGTT GAGAAGCATG 2520

CCGCATGCCA TCCTGGAGCA GACAGGGGAG AGACTGCCAG GCTGTGCCAT TGTAGGAGAC 2580

AAAGGATGCC ACCACATGCA GATGGGATCT GAAGAAACTC CTCTCTGGCT CCAAAGTGCC 2640

CCTGAAGACT CTGGATTACC ACACTCTCCC TCCCCTGGAC CTCAAAGGGA CCTGGCCAGC 2700

CAGGCCTCTC TTGTGAAACC TGAAGGTGTT CTGGGCGAGT GCCAGGCATG CCCGGCTCTG 2760

CCACAAAGGC CACACACTGC TTCGGTGAGA ACAACATCTC CACCTACCTG GGTTTGTGAC 2820

AAGGACCATC AGGTGCCAGC CCTTGCCTGC TTGCTTGGTG CTCAGGAGAG AAGCAGGTGT 2880

CAGACTCCTT CCTTGCTGGA TGCCAGCATA GAAGGTCAGA AGAAGGACTG TGCCATGCCC 2940

TTGTGTAGAG TGAAGAGCGT GATGTGTCAG CCGTCCATCT ACGCCCTGGA GAAAGACTTT 3000

CTGACCTGA 3009

Domain Profile

S: 2     eleeligsgafgkvykakwrkkevavKklreeaee..kekleklereakllsklshpnii  59

         el+elig+g fg+vy+a+w+++evavK++r++ e+  ++++e+++rea+l+++l+hpnii

Q: 111   ELKELIGAGGFGQVYRATWQGQEVAVKAARRDPEQdaAAAAESVRREARLFAMLRHPNII  170

         689*******************************9888899*******************

S: 60    ellGvcleepnlllvmeyveggsLsnvlhe..........kskreidlevlvswalqiAr  109

         +l+Gvcl++p+l+lv+e+++gg+L+++l++          ++ r+i+++vlv+wa+qiAr

Q: 171   QLRGVCLRQPHLCLVLEFARGGALNRALAAaasdprapgpRRARRIPPQVLVNWAVQIAR  230

         *****************************99*********9*******************

S: 110   GmaYLhseapkkiihrdLksknvLl.......seekktlkicdFGtarelskstkmsaag  162

         Gm YLh+ea+++i+hrdLks+n+Ll       +  +ktlki+dFG are++++t+msaag

Q: 231   GMLYLHEEAVVPILHRDLKSSNILLlekiehdDICNKTLKITDFGLAREWHRTTRMSAAG  290

         *****************************999999*************************

S: 163   tlawmaPEvlkeekvseksDvwSygvvLWelltrevPykdlselaiaykvalkglrlpip  222

         t+awmaPEv++++ +s+ sD+wSygv+LWellt+evPy++++ la+ay+va+++l+lpip

Q: 291   TYAWMAPEVIRSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIP  350

         ************************************************************

S: 223   sscpeklkkLmkecwnedpekRpsfseivksl  254

         s+cpe+++kLmkecw++dp+ Rpsf+ i+++l

Q: 351   STCPEPFAKLMKECWEQDPHIRPSFALILQQL  382

         ****************************9986

Domain Sequence
(FASTA)

ELKELIGAGG FGQVYRATWQ GQEVAVKAAR RDPEQDAAAA AESVRREARL FAMLRHPNII 60

QLRGVCLRQP HLCLVLEFAR GGALNRALAA AASDPRAPGP RRARRIPPQV LVNWAVQIAR 120

GMLYLHEEAV VPILHRDLKS SNILLLEKIE HDDICNKTLK ITDFGLAREW HRTTRMSAAG 180

TYAWMAPEVI RSSLFSKGSD IWSYGVLLWE LLTGEVPYRG IDGLAVAYGV AVNKLTLPIP 240

STCPEPFAKL MKECWEQDPH IRPSFALILQ QL 272

Keyword

ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0000186	; P:activation of MAPKK activity
GO:0000165	; P:MAPK cascade

KEGG

mmu:234878

;

InterPros

IPR011009	; Kinase-like_dom.
IPR016231	; MAPKKK9/10/11.
IPR015785	; MAPKKK9/10/11-like.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR001452	; SH3_domain.
IPR020635	; Tyr_kinase_cat_dom.

Pfam

PF07714	; Pkinase_Tyr; 1.
PF00018	; SH3_1; 1.

SMARTs

SM00326	; SH3; 1.
SM00219	; TyrKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50002	; SH3; 1.

Prints

PR00452	; SH3DOMAIN.
PR00109	; TYRKINASE.

Created Date

20-Feb-2013