EKS-MUM-00315

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00315

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/STKR	395.0	4.7E-118	244	537	294

Status

Reviewed

Ensembl Protein

ENSMUSP00000035014

UniProt Accession

Q62312; Q63947;

Protein Name

TGF-beta receptor type-2

Protein Synonyms/Alias

TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II;

Gene Name

Tgfbr2

Gene Synonyms/Alias

Tgfbr2;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000032440	ENSMUSP00000035014	ENSMUST00000035014
ENSMUSG00000032440	ENSMUSP00000062333	ENSMUST00000061101

Organism

Mus musculus

Functional Description

Transmembrane serine/threonine kinase forming with theTGF-beta type I serine/threonine kinase receptor, TGFBR1, the non- promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non- canonical, SMAD-independent TGF-beta signaling pathways (By similarity).

Protein Length

567

Protein Sequence
(FASTA)

MGRGLLRGLW PLHIVLWTRI ASTIPPHVPK SVNSDVMASD NGGAVKLPQL CKFCDVRLST 60

CDNQKSCMSN CSITAICEKP HEVCVAVWRK NDKNITLETV CHDPKLTYHG FTLEDAASPK 120

CVMKEKKRAG ETFFMCACNM EECNDYIIFS EEYTTSSPDL LLVIIQVTGV SLLPPLGIAI 180

AVIIIFYCYR VHRQQKLSPS WESSKPRKLM DFSDNCAIIL EDDRSDISST CANNINHNTE 240

LLPIELDTLV GKGRFAEVYK AKLKQNTSEQ FETVAVKIFP YEEYSSWKTE KDIFSDINLK 300

HENILQFLTA EERKTELGKQ YWLITAFHAK GNLQEYLTRH VISWEDLRKL GSSLARGIAH 360

LHSDHTPCGR PKMPIVHRDL KSSNILVKND LTCCLCDFGL SLRLDPTLSV DDLANSGQVG 420

TARYMAPEVL ESRMNLENVE SFKQTDVYSM ALVLWEMTSR CNAVGEVKDY EPPFGSKVRE 480

HPCVESMKDS VLRDRGRPEI PSFWLNHQGI QIVCETLTEC WDHDPEARLT AQCVAERFSE 540

LEHPERLSGR SCSQEKIPED GSLNTTK 567

Nucleotide Sequence
(FASTA)

ATGGGTCGGG GGCTGCTCCG GGGCCTGTGG CCGCTGCATA TCGTCCTGTG GACGCGCATC 60

GCCAGCACGA TCCCGCCGCA CGTTCCCAAG TCGGTTAACA GTGATGTCAT GGCCAGCGAC 120

AATGGCGGTG CGGTCAAGCT TCCACAGCTG TGCAAGTTTT GCGATGTGAG ACTGTCCACT 180

TGCGACAACC AGAAGTCCTG CATGAGCAAC TGCAGCATCA CGGCCATCTG TGAGAAGCCG 240

CATGAAGTCT GCGTGGCCGT GTGGAGGAAG AACGACAAGA ACATTACTCT GGAGACGGTT 300

TGCCACGACC CCAAGCTCAC CTACCACGGC TTCACTCTGG AAGATGCCGC TTCTCCCAAG 360

TGTGTCATGA AGGAAAAGAA AAGGGCGGGC GAGACTTTCT TCATGTGTGC CTGTAACATG 420

GAAGAGTGCA ACGATTACAT CATCTTTTCG GAAGAATACA CCACCAGCAG TCCCGACCTG 480

TTGTTGGTCA TTATCCAAGT GACGGGTGTC AGCCTCCTGC CTCCGCTGGG GATTGCCATA 540

GCTGTCATCA TCATCTTCTA CTGCTACCGT GTCCACCGGC AGCAGAAGCT GAGCCCGTCC 600

TGGGAGAGCA GCAAGCCCCG GAAACTGATG GATTTCAGTG ACAATTGTGC CATCATCCTG 660

GAGGACGACC GCTCCGACAT CAGCTCCACG TGCGCCAACA ACATCAACCA CAACACGGAG 720

CTGCTGCCCA TCGAGCTGGA CACGCTGGTG GGGAAGGGCC GCTTCGCCGA GGTCTACAAG 780

GCCAAGCTGA AGCAGAACAC CTCAGAGCAG TTTGAGACCG TGGCTGTCAA GATCTTCCCC 840

TACGAGGAGT ACTCCTCGTG GAAAACAGAG AAGGACATCT TCTCCGATAT CAACCTGAAG 900

CATGAGAACA TCCTGCAGTT CCTGACGGCC GAGGAGCGGA AGACAGAGCT GGGCAAGCAG 960

TACTGGCTGA TCACGGCGTT CCACGCGAAG GGCAACCTGC AGGAGTACCT CACGAGGCAT 1020

GTCATCAGCT GGGAGGACCT GAGGAAGCTG GGCAGCTCCC TGGCCCGGGG CATCGCTCAT 1080

CTCCACAGTG ACCACACTCC TTGTGGGAGG CCCAAGATGC CCATTGTTCA CAGGGACCTC 1140

AAGAGCTCTA ACATCCTAGT GAAGAACGAC TTGACCTGTT GCCTGTGTGA CTTCGGGCTG 1200

TCCTTGCGCC TGGACCCTAC TCTGTCTGTG GATGACCTGG CCAACAGCGG GCAGGTGGGA 1260

ACGGCAAGAT ACATGGCCCC GGAAGTTCTA GAATCCAGGA TGAATCTGGA AAACGTGGAG 1320

TCGTTCAAGC AGACGGATGT CTACTCCATG GCTCTGGTAC TCTGGGAAAT GACGTCCCGC 1380

TGCAATGCTG TGGGAGAAGT GAAGGATTAC GAGCCCCCAT TTGGTTCCAA GGTGCGGGAG 1440

CACCCCTGTG TGGAGAGCAT GAAAGACAGT GTGCTGAGAG ACCGAGGGCG GCCGGAAATT 1500

CCCAGCTTCT GGCTCAACCA CCAGGGCATC CAGATCGTGT GTGAGACTTT GACCGAGTGC 1560

TGGGACCATG ACCCCGAAGC CCGTCTCACA GCACAGTGTG TGGCAGAGCG CTTCAGTGAG 1620

CTGGAGCATC CGGAGAGACT CTCTGGGAGG AGCTGCTCCC AGGAGAAGAT TCCAGAAGAT 1680

GGCTCGCTGA ACACTACCAA ATAG 1704

Domain Profile

S: 1     lklleligkGrygeVwkaklrg......eevAvKifstedeaswkrEkeiyqtvllrhen  54

         ++l +l+gkGr++eV+kakl++      e+vAvKif++e+ +swk+Ek+i++ ++l+hen

Q: 244   IELDTLVGKGRFAEVYKAKLKQntseqfETVAVKIFPYEEYSSWKTEKDIFSDINLKHEN  303

         678899***************99999999*******************************

S: 55    ilqfiaadkkeedsltelllvteyhekgsLsdyLkretldveellrlalslasGlahLHe  114

         ilqf++a+++++++ ++++l+t++h kg+L++yL+r+++++e+l +l +sla+G+ahLH+

Q: 304   ILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHS  363

         ************************************************************

S: 115   eivgtkgkkKpaiaHRDlkskNilvkkdltcciaDlGLalkleeekeeldlaansqvGtk  174

         + ++ +g+ K+ i+HRDlks Nilvk+dltcc++D+GL+l+l+ + ++ dla++ qvGt

Q: 364   D-HTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTA  422

         *.8999******************************************************

S: 175   RYmaPEvleealnlkdfeafkraDvYslgLvlWEvasRceevdeeveeyklpfeevvgsd  234

         RYmaPEvle+++nl+++e+fk++DvYs++LvlWE++sRc++  +ev++y++pf ++v+++

Q: 423   RYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNA-VGEVKDYEPPFGSKVREH  481

         *****************************************.999***************

S: 235   PsleemkevvvekklrPkipeawkkkealkelsklleecWdadpeaRltalrvkkr  290

         P++e+mk+ v++++ rP+ip+ w +++ ++ ++++l+ecWd+dpeaRlta++v++r

Q: 482   PCVESMKDSVLRDRGRPEIPSFWLNHQGIQIVCETLTECWDHDPEARLTAQCVAER  537

         *****************************************************987

Domain Sequence
(FASTA)

IELDTLVGKG RFAEVYKAKL KQNTSEQFET VAVKIFPYEE YSSWKTEKDI FSDINLKHEN 60

ILQFLTAEER KTELGKQYWL ITAFHAKGNL QEYLTRHVIS WEDLRKLGSS LARGIAHLHS 120

DHTPCGRPKM PIVHRDLKSS NILVKNDLTC CLCDFGLSLR LDPTLSVDDL ANSGQVGTAR 180

YMAPEVLESR MNLENVESFK QTDVYSMALV LWEMTSRCNA VGEVKDYEPP FGSKVREHPC 240

VESMKDSVLR DRGRPEIPSF WLNHQGIQIV CETLTECWDH DPEARLTAQC VAER 294

Keyword

Alternative splicing; Apoptosis; ATP-binding; Cell membrane; Complete proteome; Differentiation; Disulfide bond; Glycoprotein; Growth regulation; Kinase; Magnesium; Manganese; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Serine/threonine-protein kinase; Signal; Transferase; Transmembrane; Transmembrane helix.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005901	; C:caveola
GO:0009897	; C:external side of plasma membrane
GO:0016021	; C:integral to membrane
GO:0043235	; C:receptor complex
GO:0005524	; F:ATP binding
GO:0005539	; F:glycosaminoglycan binding
GO:0046872	; F:metal ion binding
GO:0004702	; F:receptor signaling protein serine/threonine kinase activity
GO:0046332	; F:SMAD binding
GO:0005026	; F:transforming growth factor beta receptor activity, type II
GO:0005024	; F:transforming growth factor beta-activated receptor activity
GO:0032147	; P:activation of protein kinase activity
GO:0006915	; P:apoptotic process
GO:0007420	; P:brain development
GO:0060434	; P:bronchus morphogenesis
GO:0051216	; P:cartilage development
GO:0048701	; P:embryonic cranial skeleton morphogenesis
GO:0035162	; P:embryonic hemopoiesis
GO:0007369	; P:gastrulation
GO:0007507	; P:heart development
GO:0001701	; P:in utero embryonic development
GO:0002088	; P:lens development in camera-type eye
GO:0060463	; P:lung lobe morphogenesis
GO:0060443	; P:mammary gland morphogenesis
GO:0043011	; P:myeloid dendritic cell differentiation
GO:0060021	; P:palate development
GO:0060389	; P:pathway-restricted SMAD protein phosphorylation
GO:0001569	; P:patterning of blood vessels
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0018107	; P:peptidyl-threonine phosphorylation
GO:0002663	; P:positive regulation of B cell tolerance induction
GO:0010634	; P:positive regulation of epithelial cell migration
GO:0002053	; P:positive regulation of mesenchymal cell proliferation
GO:0051138	; P:positive regulation of NK T cell differentiation
GO:2000379	; P:positive regulation of reactive oxygen species metabolic process
GO:0002666	; P:positive regulation of T cell tolerance induction
GO:0002651	; P:positive regulation of tolerance induction to self antigen
GO:0040008	; P:regulation of growth
GO:0070723	; P:response to cholesterol
GO:0042493	; P:response to drug
GO:0023014	; P:signal transduction by phosphorylation
GO:0007224	; P:smoothened signaling pathway
GO:0060440	; P:trachea formation
GO:0007179	; P:transforming growth factor beta receptor signaling pathway
GO:0001570	; P:vasculogenesis

KEGG

mmu:21813

;

InterPros

IPR000333	; Activin_II/TGFBeta-II_recpt.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR017194	; Transform_growth_fac-b_typ-2.
IPR015013	; Transforming_GF_b_rcpt_2_ecto.

Pfam

PF08917	; ecTbetaR2; 1.
PF00069	; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00653

; ACTIVIN2R.

Created Date

20-Feb-2013