EKS-MUM-00467

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00467

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/ULK	328.0	1.2E-97	4	254	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000120020

UniProt Accession

Q69ZM6; Q6PDI0; Q80XQ6;

Protein Name

Serine/threonine-protein kinase 36

Protein Synonyms/Alias

Fused homolog;

Gene Name

Stk36

Gene Synonyms/Alias

Stk36; Kiaa1278;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000033276	ENSMUSP00000109324	ENSMUST00000113694
ENSMUSG00000033276	ENSMUSP00000084430	ENSMUST00000087183
ENSMUSG00000033276	ENSMUSP00000084433	ENSMUST00000087186
ENSMUSG00000033276	ENSMUSP00000120020	ENSMUST00000148456

Organism

Mus musculus

Functional Description

Serine/threonine protein kinase which plays an importantrole in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.

Protein Length

1048

Protein Sequence
(FASTA)

MEKYHVLEMI GEGSFGRVYK GRKKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLWH 60

PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR 120

ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT 180

ADLWSVGCIL YELAVGTPPF YTTSIFQLVS LILKDPVRWP STISSCFKNF LQGLLTKDPR 240

QRLSWPDLLH HPFIAGRVTI ITEPAGSDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR 300

ILRQACKLMA EEAKQKEDQN AGSALEQEDG LCKVTPSTAP VPGLKATPQE SSLLAGILAS 360

EMKNNWEDWG AGEAPRTSRE NHINLECEQG FPEPRPEAMG RQSTDVVDPE NEEPDSDDEW 420

QRLLETSEPG PVQLKSPLTL LCNPDFCQRI QSQLRGTGEQ ILKGVLDGVS HLLPVLRILS 480

SLLSSCNDSV LLYSFCQEAG LPELPLSLLR YSQESSSIQQ QPWYGALLRD LVAVVQAYFS 540

CTFNLERSQT GDSLQVFQEA ASLFLDLLGK LLAQSDDSEQ TFRRDSLMCF AVLCEAVDGN 600

SWAVSKAFYS SLLTTQRAVL DGLLHGLTVP QLPFHTPPGA PQVSQPLREQ SEDVPGAISS 660

ALAAMCTAPV GLPSCWDAKE QVSWHLANQL TEDSSQLRPS LISGLRHHVL CLHLLKVLYA 720

CCYISERLCH ILGQEPLALE SLLMLVQGKV KVADWEESTE VALYLLSLLV FRLQDLPSGM 780

EKLGSEVATL FTHSHVVSLV NAAACLLGQL GQQGVTFDLQ PREWIAAAAH ALSAPAEVRL 840

TPPYSCGFYD GLLILLLQLL MQGKPGLIRD VVGSEVWTIL WHRFSMALRL PEEVSAQEDD 900

LLLSSPSSLE PDWTLISPQG MAALLSLAMA IFTQESQLCL SHLSQHGSVL MLTLKHLLSP 960

SFLHHLSQAP QGPEFLPVVV LSVCKLLCFP FALDVDADLL VGVLADLRAS EVVVCLLQVC 1020

CHHLSLLQAE LPIGLLTRLA LTDSASLK 1048

Nucleotide Sequence
(FASTA)

ATGGAAAAGT ACCACGTTTT GGAGATGATT GGAGAAGGCT CTTTTGGGAG AGTGTATAAG 60

GGCCGAAAAA AATACAGTGC TCAGGTGGTG GCCTTGAAGT TCATCCCCAA ACTGGGGCGC 120

TCAGAGAAAG AGCTGAGGAA TCTGCAACGA GAGATTGAAA TCATGCGGGG TCTGTGGCAT 180

CCCAACATTG TGCATATGCT CGACAGCTTT GAGACTGACA AAGAGGTGGT GGTGGTGACA 240

GACTACGCTG AAGGAGAACT CTTTCAGATT CTGGAAGATG ATGGAAAACT TCCTGAAGAC 300

CAGGTTCAGG CCATCGCTGC CCAGTTGGTG TCAGCTCTGT ACTACCTGCA TTCCCACCGC 360

ATCCTACACC GCGACATGAA ACCGCAGAAC ATTCTTCTCG CCAAGGGTGG TGGCATTAAA 420

CTTTGTGACT TTGGATTCGC CCGAGCTATG AGCACCAACA CCATGGTGCT GACGTCGATC 480

AAAGGCACAC CGCTCTATAT GTCTCCAGAG CTGGTGGAGG AGCGACCATA TGACCACACC 540

GCAGACCTCT GGTCTGTGGG CTGCATCCTG TATGAGCTGG CTGTCGGCAC GCCTCCCTTC 600

TACACCACCA GCATCTTTCA GCTGGTTAGC CTCATTCTCA AGGACCCTGT GCGCTGGCCC 660

TCCACCATTA GTTCCTGCTT CAAGAACTTC TTGCAGGGGC TGCTCACCAA GGACCCCCGG 720

CAGCGTCTGT CCTGGCCAGA CCTCTTACAT CACCCCTTTA TTGCCGGCCG TGTCACCATC 780

ATAACTGAAC CAGCAGGCTC CGATTTGGGC ACCCCATTTA CTAGTCGCCT ACCCCCAGAA 840

CTTCAGGTCC TCAAGGATGA ACAGGCGCAT CGGCTCGCAC CCAAGGGTAA CCAGTCTCGC 900

ATCCTGCGCC AGGCCTGTAA ACTCATGGCT GAAGAAGCCA AGCAGAAGGA AGACCAAAAT 960

GCAGGATCTG CCCTTGAACA AGAAGACGGG CTCTGCAAGG TGACACCCAG CACAGCCCCC 1020

GTGCCTGGAC TAAAGGCCAC TCCTCAGGAG TCAAGCCTCT TGGCTGGTAT ACTGGCTTCA 1080

GAAATGAAGA ACAACTGGGA AGACTGGGGG GCTGGAGAAG CACCCCGTAC CTCTCGGGAA 1140

AACCACATCA ACCTGGAGTG TGAACAAGGC TTCCCAGAGC CGAGGCCAGA GGCGATGGGC 1200

CGGCAGAGCA CTGATGTAGT GGATCCTGAA AATGAGGAGC CAGACAGTGA TGATGAGTGG 1260

CAACGCCTAC TAGAGACCAG CGAGCCTGGG CCTGTGCAGC TGAAGTCCCC CCTCACCCTG 1320

TTGTGTAACC CTGACTTCTG CCAGCGCATC CAGAGTCAGC TGCGCGGGAC TGGCGAGCAG 1380

ATCCTGAAAG GCGTTCTGGA TGGTGTGTCC CACCTCCTTC CTGTACTCCG CATCCTGAGT 1440

AGTCTCCTAT CCAGCTGCAA TGACTCTGTG CTCTTGTATT CCTTCTGCCA GGAGGCAGGA 1500

CTGCCTGAGC TGCCTCTCAG CCTTCTTAGG TACAGCCAGG AGAGTAGCAG CATCCAGCAG 1560

CAACCTTGGT ATGGGGCGCT CTTACGGGAC CTGGTGGCTG TGGTTCAGGC CTACTTTTCG 1620

TGCACCTTCA ATCTGGAGAG GAGCCAGACA GGTGACAGCC TACAGGTGTT TCAGGAGGCC 1680

GCCAGCCTCT TTTTGGACCT GTTGGGGAAG CTGCTGGCCC AATCAGATGA TTCCGAGCAG 1740

ACATTTCGAA GGGATAGCCT TATGTGCTTT GCTGTCTTGT GTGAAGCTGT GGACGGAAAC 1800

AGCTGGGCCG TCTCCAAAGC CTTCTACTCC AGCCTGTTGA CCACACAGCG CGCTGTGTTG 1860

GATGGCCTCC TTCACGGCCT GACAGTCCCA CAGCTTCCTT TCCACACACC ACCAGGAGCC 1920

CCACAAGTGA GCCAGCCGCT GCGGGAGCAG AGTGAAGATG TACCTGGAGC CATCTCTTCT 1980

GCCCTGGCAG CCATGTGTAC TGCTCCTGTG GGGCTGCCCA GCTGTTGGGA TGCCAAGGAG 2040

CAGGTCTCTT GGCATTTGGC CAACCAGCTA ACTGAAGACA GCAGCCAACT GAGGCCATCC 2100

CTCATCTCTG GTCTGCGACA CCATGTCCTG TGCCTCCACC TTCTCAAGGT TCTCTACGCC 2160

TGCTGCTATA TTAGTGAGCG CCTGTGCCAT ATTCTGGGGC AAGAGCCCCT GGCCTTGGAG 2220

TCGCTGCTGA TGTTAGTCCA GGGCAAGGTG AAAGTAGCAG ATTGGGAAGA GTCCACTGAG 2280

GTGGCACTTT ACCTCCTGTC CCTTCTTGTC TTTCGGCTCC AAGATCTGCC TTCAGGGATG 2340

GAGAAGCTGG GCAGTGAGGT TGCTACTCTC TTTACCCACT CACATGTCGT CTCTCTTGTG 2400

AATGCGGCAG CCTGTCTCTT AGGACAGCTT GGTCAGCAAG GGGTGACCTT TGACCTCCAG 2460

CCCCGGGAAT GGATTGCTGC AGCTGCACAT GCCTTGTCTG CCCCTGCAGA GGTCCGGCTG 2520

ACTCCGCCGT ATAGTTGTGG CTTCTATGAT GGCCTCCTCA TTCTCCTGCT TCAGCTTCTT 2580

ATGCAGGGGA AGCCTGGCCT GATCAGGGAT GTGGTTGGTT CAGAGGTGTG GACCATTCTG 2640

TGGCACCGCT TTTCCATGGC CCTGAGGCTG CCCGAGGAGG TGTCTGCCCA GGAAGACGAC 2700

CTGCTACTAT CAAGTCCCTC CAGCCTAGAG CCAGACTGGA CACTGATTTC ACCCCAAGGC 2760

ATGGCAGCCT TGCTGAGCCT GGCCATGGCC ATCTTCACCC AGGAGTCCCA GTTATGCCTG 2820

AGCCACCTGT CCCAGCATGG CAGTGTCCTC ATGCTGACCC TGAAGCACCT GCTTTCACCC 2880

AGCTTCTTGC ACCACCTGAG CCAGGCGCCG CAGGGGCCCG AGTTTCTCCC CGTTGTGGTG 2940

CTCTCCGTGT GCAAGCTGCT CTGCTTCCCT TTTGCCCTGG ATGTGGATGC TGACCTCCTT 3000

GTAGGTGTCT TGGCTGACCT CAGGGCCTCG GAAGTGGTAG TCTGCCTGCT GCAGGTCTGC 3060

TGCCACCACC TTTCGTTGTT ACAAGCAGAG CTGCCCATTG GCCTCCTTAC ACGCCTGGCC 3120

CTCACGGATT CTGCCTCTCT CAAGC 3145

Domain Profile

S: 1     ysleeeiGkGsfavvykgrlrkktsqvvAikvvekkkl.kkslenlekeikilkelkhen  59

         y++ e+iG+Gsf+ vykgr +k ++qvvA+k++ k ++ +k+l+nl++ei+i++ l+h+n

Q: 4     YHVLEMIGEGSFGRVYKGR-KKYSAQVVALKFIPKLGRsEKELRNLQREIEIMRGLWHPN  62

         8999***************.9*****************9*********************

S: 60    ivalldveetskevylvmeycaggdLakllkqkkeLsedtvreflqqlasalkaLhskki  119

         iv++ld++et+kev++v++y++ g+L ++l+++++L+ed+v+++++ql+sal++Lhs++i

Q: 63    IVHMLDSFETDKEVVVVTDYAE-GELFQILEDDGKLPEDQVQAIAAQLVSALYYLHSHRI  121

         **********************.*************************************

S: 120   vhrDlkPqniLlsyadakkelkkvtlkladFglarvlkeesma.atlkGsplYmaPEvvk  178

         +hrD+kPqniLl+        k +++kl+dFg+ar++++++m  +++kG+plYm+PE+v+

Q: 122   LHRDMKPQNILLA--------KGGGIKLCDFGFARAMSTNTMVlTSIKGTPLYMSPELVE  173

         *************........89********************9****************

S: 179   eqqydakaDlWSiGtilyellvGkpPfaaesiseLvekiekndvleppipkeisselkdl  238

         e+ yd++aDlWS+G+ilyel+vG+pPf+++si++Lv+ i k++v   ++p++iss++k++

Q: 174   ERPYDHTADLWSVGCILYELAVGTPPFYTTSIFQLVSLILKDPV---RWPSTISSCFKNF  230

         ********************************************...*************

S: 239   lqsLLqrdpkkRisfeelfahpfl  262

         lq+LL++dp++R+s+ +l++hpf+

Q: 231   LQGLLTKDPRQRLSWPDLLHHPFI  254

         **********************96

Domain Sequence
(FASTA)

YHVLEMIGEG SFGRVYKGRK KYSAQVVALK FIPKLGRSEK ELRNLQREIE IMRGLWHPNI 60

VHMLDSFETD KEVVVVTDYA EGELFQILED DGKLPEDQVQ AIAAQLVSAL YYLHSHRILH 120

RDMKPQNILL AKGGGIKLCD FGFARAMSTN TMVLTSIKGT PLYMSPELVE ERPYDHTADL 180

WSVGCILYEL AVGTPPFYTT SIFQLVSLIL KDPVRWPSTI SSCFKNFLQG LLTKDPRQRL 240

SWPDLLHHPF I 251

Keyword

Alternative splicing; ATP-binding; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Developmental protein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0008134	; F:transcription factor binding
GO:0007420	; P:brain development
GO:0042384	; P:cilium assembly
GO:0003351	; P:epithelial cilium movement
GO:0007228	; P:positive regulation of hh target transcription factor activity
GO:0045880	; P:positive regulation of smoothened signaling pathway
GO:0009791	; P:post-embryonic development

KEGG

mmu:269209

;

InterPros

IPR011989	; ARM-like.
IPR016024	; ARM-type_fold.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013