EKS-MUM-00412

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00412

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
TKL/MLK	320.2	2.4E-95	169	406	238

Status

Reviewed

Ensembl Protein

ENSMUSP00000047388

UniProt Accession

Q1HKZ5; Q8BKN0;

Protein Name

Mitogen-activated protein kinase kinase kinase 13

Protein Synonyms/Alias

Gene Name

Map3k13

Gene Synonyms/Alias

Map3k13;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000033618	ENSMUSP00000047388	ENSMUST00000042065

Organism

Mus musculus

Functional Description

Activates the JUN N-terminal pathway through activationof the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B (By similarity).

Protein Length

959

Protein Sequence
(FASTA)

MANPQEHLSC SSLPHLPLTE NKTSGGRNEL AAMGNHPSPK LPEDPQERGA IQSELMEITG 60

SPISTTVLTS VSEDSRGQFE NSVLQLREQD ESEMTLSLGN SNTVDGENTN GPEDIKIQFS 120

RSGSGSGGFL EGLFGCLRPV WNIIGKAYST DYKLQQQDTW EVPFEEISEL QWLGSGAQGA 180

VFLGKFRAEE VAIKKVREQN ETDIKHLRKL KHPNIIAFKG VCTQAPCYCI IMEYCAHGQL 240

YEVLRAGRKI TPRLLVDWST GIASGMNYLH LHKIIHRDLK SPNVLVTHTD AVKISDFGTS 300

KELSDKSTKM SFAGTVAWMA PEVIRNEPVS EKVDIWSFGV VLWELLTGEI PYKDVDSSAI 360

IWGVGSNSLH LPVPSTCPDG FKILMKQTWQ SKPRNRPSFR QTLMHLDIAS ADVLATPQET 420

YFKSQAEWRE EVKKHFEKIK SEGTCIHRLD EELIRRRREE LRHALDIREH YERKLERANN 480

LYMELSAIML QLEMREKELL KREQAVEKKY PGTYKRHPVR PIIHPNAMEK LMKRKGVPHK 540

AGVQTKRPDL LRSEGIPSTE AVPTASPLSG SPKMSTASSR SRYRSKPRHR RGNSRGSHSD 600

FAAILKTQPA QENSPHPTYM HHTQAQCASV HQHNPLQQQY QQIPPAQPQS RHPRLNAHGQ 660

DIATCANNLR YFGPAAALRS PLSNHAQRQM PGSSPDLIST AMAADWRNSE LDQDQVGPWG 720

CCQAEPYDPC FQCRPEHSGS LDVPTTEPVG RSPDLSSSPA HNPLSGNAQG SERTGANGFS 780

GCQSGISHQF TPPMLPQKTR PLQKSGDDSS EEEGEVDSEV EFPRRQRPHR CISSYQSYST 840

FSSENFSVSD GEEGNTSDHS NSPDESANRR QDRLAETLDD LLSQTPEAPI EISSHSDGLS 900

DKECAVRRVK TQMSLGKLCA EERGYENPVQ FGDSDCDSSE GECSDATVRT SKNYSSATW 959

Nucleotide Sequence
(FASTA)

ATGGCCAACC CTCAGGAGCA CCTGAGCTGC TCCTCTTTGC CACACTTGCC CTTGACTGAG 60

AACAAAACCT CTGGTGGACG AAATGAGCTT GCAGCTATGG GGAACCACCC TTCACCCAAG 120

CTCCCTGAGG ACCCTCAGGA GAGGGGGGCT ATACAGTCAG AGCTGATGGA GATCACAGGC 180

AGCCCGATCT CCACAACAGT GTTGACCAGT GTAAGCGAGG ATTCTAGGGG CCAGTTTGAG 240

AACAGTGTTC TTCAGCTAAG GGAACAGGAT GAGTCAGAGA TGACCCTGTC TCTGGGGAAC 300

AGCAACACTG TGGATGGAGA GAACACGAAC GGACCAGAAG ACATCAAGAT TCAGTTCAGC 360

AGGTCAGGCA GCGGCAGCGG CGGGTTTCTC GAAGGACTGT TTGGATGTCT CAGGCCCGTG 420

TGGAATATCA TTGGGAAGGC ATATTCCACT GATTACAAGC TGCAGCAGCA AGATACTTGG 480

GAAGTGCCAT TTGAGGAAAT CTCAGAGCTG CAATGGCTGG GTAGTGGAGC CCAAGGAGCT 540

GTCTTCCTGG GCAAATTCCG AGCAGAGGAG GTGGCCATCA AGAAGGTGAG AGAACAAAAT 600

GAGACGGACA TCAAGCATCT GAGGAAGCTG AAGCACCCTA ACATCATCGC CTTCAAGGGT 660

GTTTGCACTC AGGCCCCGTG CTACTGCATC ATCATGGAGT ACTGTGCCCA CGGACAGCTG 720

TACGAGGTCC TGAGAGCTGG CCGGAAGATC ACGCCCCGGC TGCTTGTGGA CTGGTCCACG 780

GGAATCGCCA GTGGAATGAA TTATCTCCAT CTCCACAAGA TTATACACCG TGACCTCAAG 840

TCGCCCAATG TTTTAGTGAC TCACACAGAT GCAGTGAAAA TTTCAGACTT TGGCACATCT 900

AAGGAACTCA GTGATAAAAG CACCAAGATG TCCTTCGCTG GCACCGTCGC GTGGATGGCA 960

CCAGAGGTGA TCCGGAATGA GCCTGTCTCG GAGAAGGTGG ACATATGGTC ATTTGGTGTG 1020

GTACTTTGGG AGCTGCTGAC AGGGGAGATT CCCTACAAGG ATGTGGACTC TTCCGCCATC 1080

ATTTGGGGTG TCGGCAGCAA CAGCTTGCAT CTCCCAGTTC CTTCCACTTG CCCGGATGGA 1140

TTCAAAATCC TTATGAAACA GACATGGCAG AGTAAGCCTC GCAACCGACC TTCTTTCAGG 1200

CAGACGCTCA TGCATCTGGA TATCGCGTCT GCAGATGTGC TCGCCACCCC TCAAGAAACT 1260

TACTTCAAGT CTCAGGCTGA ATGGAGAGAA GAAGTGAAGA AACATTTTGA GAAAATCAAA 1320

AGTGAAGGGA CCTGTATACA CCGATTAGAT GAAGAACTGA TTCGCCGGCG CAGGGAGGAG 1380

CTCAGGCATG CTCTGGATAT TCGGGAGCAC TACGAGAGAA AGCTCGAACG GGCTAACAAT 1440

CTGTACATGG AGCTGAGCGC CATCATGCTG CAGCTGGAGA TGCGCGAGAA GGAGCTCCTT 1500

AAGCGAGAGC AAGCAGTGGA AAAGAAGTAC CCTGGAACCT ACAAACGACA CCCTGTCCGT 1560

CCAATCATCC ACCCGAATGC CATGGAGAAA CTCATGAAAA GGAAGGGTGT GCCTCACAAG 1620

GCTGGGGTGC AGACTAAGCG GCCAGACCTG TTGCGATCCG AAGGCATTCC TAGTACAGAA 1680

GCGGTTCCCA CTGCATCCCC CCTGTCTGGA AGTCCCAAAA TGTCCACCGC AAGCAGCAGA 1740

AGCCGGTACC GAAGCAAACC ACGTCACCGC CGAGGGAACA GTAGAGGCAG CCATAGCGAC 1800

TTCGCAGCAA TCTTGAAAAC CCAGCCCGCC CAGGAAAATT CACCCCACCC CACCTACATG 1860

CACCACACTC AGGCTCAGTG CGCTTCAGTC CATCAACATA ACCCTCTCCA GCAGCAATAC 1920

CAGCAAATTC CTCCTGCCCA GCCTCAGAGC CGGCATCCCA GACTCAATGC GCATGGGCAG 1980

GACATTGCAA CCTGCGCCAA TAACCTGAGG TACTTTGGCC CCGCAGCGGC CCTGCGGAGT 2040

CCACTCAGCA ACCATGCACA AAGACAGATG CCTGGCTCAA GCCCCGACCT CATCTCCACA 2100

GCCATGGCTG CAGATTGGAG AAATTCTGAA CTTGATCAAG ACCAAGTTGG ACCCTGGGGC 2160

TGTTGTCAGG CTGAACCCTA TGATCCCTGT TTCCAGTGCA GGCCAGAACA TTCTGGGTCT 2220

TTAGATGTTC CCACTACTGA GCCAGTGGGG AGGAGCCCAG ACCTTTCCAG TTCACCAGCA 2280

CACAATCCTC TTTCAGGAAA TGCCCAAGGC TCTGAGAGAA CAGGGGCAAA TGGATTCAGT 2340

GGCTGTCAGT CTGGTATTTC TCATCAATTC ACACCCCCAA TGCTACCTCA GAAGACACGG 2400

CCCCTTCAGA AGAGTGGCGA TGACTCCTCA GAAGAGGAAG GGGAAGTGGA CAGTGAAGTA 2460

GAATTTCCTC GACGACAGAG GCCTCACCGC TGCATTAGCA GTTACCAGTC ATACTCAACC 2520

TTTAGCTCCG AGAACTTCTC TGTGTCTGAT GGAGAGGAGG GCAACACTAG TGACCACTCG 2580

AACAGTCCCG ATGAATCCGC CAACAGACGG CAGGACCGCC TGGCAGAGAC GCTGGACGAC 2640

CTGCTGTCTC AGACACCCGA GGCCCCCATC GAGATCTCCT CCCACTCAGA TGGGCTGTCT 2700

GACAAGGAGT GTGCTGTTCG GCGCGTGAAG ACACAGATGT CCCTAGGCAA GCTGTGTGCC 2760

GAGGAGCGCG GCTACGAGAA CCCTGTGCAG TTTGGAGACT CGGACTGTGA CTCTTCAGAG 2820

GGAGAGTGTT CCGATGCCAC AGTTAGGACC AGCAAGAACT ACAGCTCTGC CACCTGGTGA 2880

Domain Profile

S: 3     leeligsgafgkvykakwrkkevavKklreeaeekekleklereakllsklshpniiell  62

         + +++gsga+g+v+ +k+r++eva+Kk+re++e+         ++k+l+kl+hpnii+++

Q: 169   ELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNET---------DIKHLRKLKHPNIIAFK  219

         6789**************************9999.........*****************

S: 63    GvcleepnlllvmeyveggsLsnvlhekskreidlevlvswalqiArGmaYLhseapkki  122

         Gvc++ p+++++mey+++g+L++vl++   r+i+++ lv+w++ iA+Gm+YLh +   ki

Q: 220   GVCTQAPCYCIIMEYCAHGQLYEVLRA--GRKITPRLLVDWSTGIASGMNYLHLH---KI  274

         ***************************..99************************...9*

S: 123   ihrdLksknvLlseekktlkicdFGtarelsk.stkmsaagtlawmaPEvlkeekvseks  181

         ihrdLks+nvL+ ++++ +ki+dFGt++els+ stkms+agt+awmaPEv+++e+vsek+

Q: 275   IHRDLKSPNVLV-THTDAVKISDFGTSKELSDkSTKMSFAGTVAWMAPEVIRNEPVSEKV  333

         ************.999***************99***************************

S: 182   DvwSygvvLWelltrevPykdlselaiaykvalkglrlpipsscpeklkkLmkecwnedp  241

         D+wS+gvvLWellt+e+Pykd+++ ai+++v++++l+lp+ps+cp+ +k Lmk++w+++p

Q: 334   DIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQSKP  393

         ************************************************************

S: 242   ekRpsfseivksl  254

         ++Rpsf++ +++l

Q: 394   RNRPSFRQTLMHL  406

         *******998775

Domain Sequence
(FASTA)

ELQWLGSGAQ GAVFLGKFRA EEVAIKKVRE QNETDIKHLR KLKHPNIIAF KGVCTQAPCY 60

CIIMEYCAHG QLYEVLRAGR KITPRLLVDW STGIASGMNY LHLHKIIHRD LKSPNVLVTH 120

TDAVKISDFG TSKELSDKST KMSFAGTVAW MAPEVIRNEP VSEKVDIWSF GVVLWELLTG 180

EIPYKDVDSS AIIWGVGSNS LHLPVPSTCP DGFKILMKQT WQSKPRNRPS FRQTLMHL 238

Keyword

ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0008385	; C:IkappaB kinase complex
GO:0016020	; C:membrane
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0007254	; P:JNK cascade
GO:0051092	; P:positive regulation of NF-kappaB transcription factor activity
GO:0046777	; P:protein autophosphorylation

KEGG

mmu:71751

;

InterPros

IPR011009	; Kinase-like_dom.
IPR017419	; MAP3K12_MAP3K13.
IPR000719	; Prot_kinase_cat_dom.
IPR001245	; Ser-Thr/Tyr_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF07714

; Pkinase_Tyr; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

PR00109

; TYRKINASE.

Created Date

20-Feb-2013