EKS-MUM-00334

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00334

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Atypical/Alpha	282.8	4.3E-84	120	317	198

Status

Reviewed

Ensembl Protein

ENSMUSP00000102096

UniProt Accession

O08796;

Protein Name

Eukaryotic elongation factor 2 kinase

Protein Synonyms/Alias

eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase;

Gene Name

Eef2k

Gene Synonyms/Alias

Eef2k;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000035064	ENSMUSP00000114782	ENSMUST00000146482
ENSMUSG00000035064	ENSMUSP00000102097	ENSMUST00000106488
ENSMUSG00000035064	ENSMUSP00000115486	ENSMUST00000143322
ENSMUSG00000035064	ENSMUSP00000102098	ENSMUST00000106489
ENSMUSG00000035064	ENSMUSP00000102096	ENSMUST00000106487
ENSMUSG00000035064	ENSMUSP00000046595	ENSMUST00000047875
ENSMUSG00000035064	ENSMUSP00000119567	ENSMUST00000143279

Organism

Mus musculus

Functional Description

Threonine kinase that regulates protein synthesis bycontrolling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Protein Length

614

Protein Sequence
(FASTA)

MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ NVSSKVQSYY 60

SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE FHLEDIATEH ATRHRYNAVT 120

GEWLKDEVLI KMASQPFGRG AMRECFRTKK LSNFLHAQQW KGASNYVAKR YIEPVDRSVY 180

FEDVQLQMEA KLWGEDYNRH KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS 240

NSGFVRDDNI RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG 300

VRGMALFFYS HACNRICQSM GLTPFDLDNM GPRDHDRMDN HRDSENSGDS GYPSEKRSDL 360

DDPEPREHGH SNGNRRHESD EDSLGSSGRV CVETWNLLNP SRLHLPRPSA VALEVQRLNA 420

LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE KDEEWDRESA IFHLEHAADL GELEAIVGLG 480

LMYSQLPHHI LADVSLKETE ENKTKGFDYL LKAAEAGDRH SMILVARAFD TGLNLSPDRC 540

QDWSEALHWY NTALETTDCD EGGEYDGIQD EPQYALLARE AEMLLTGGFG LDKNPQRSEM 600

ESHVGQWTSN SLCS 614

Nucleotide Sequence
(FASTA)

ATGGCAGACG AAGACCTCAT CTTCTGCCTG GAAGGTGTTG ACGGTGGCAG GTGCTCCCGA 60

GCTGGCCACA ATGCGGACTC TGACACAGAC AGTGACGATG ATGAGGGCTA TTTCATCTGC 120

CCCATCACTG ATGACCACAT GTCCAATCAG AATGTCAGCT CCAAAGTCCA GAGCTACTAT 180

AGCAACCTAA CAAAAACAGA GTGCGGCTCC ACAGGGTCAC CAGCCAGCTC CTTCCACTTC 240

AAGGAAGCCT GGAAGCATGC GATCGAGAAA GCCAAGCACA TGCCTGACCC CTGGGCTGAA 300

TTCCATCTCG AGGACATCGC CACAGAACAT GCTACTCGGC ACAGGTACAA CGCTGTCACC 360

GGGGAATGGC TGAAAGACGA GGTTCTGATC AAGATGGCGT CTCAGCCCTT CGGCCGTGGA 420

GCAATGAGGG AGTGCTTCAG GACGAAGAAA CTCTCCAACT TCTTGCACGC CCAGCAATGG 480

AAGGGGGCCT CCAACTACGT GGCCAAGCGC TACATCGAGC CGGTGGACAG GAGCGTGTAC 540

TTTGAGGATG TGCAGCTCCA GATGGAGGCG AAGCTCTGGG GGGAGGATTA CAATCGGCAC 600

AAGCCCCCCA AGCAGGTGGA TATCATGCAG ATGTGCATCA TTGAGCTAAA GGACAGACCA 660

GGCCAGCCCC TCTTCCACTT GGAGCACTAC ATTGAGGGCA AGTACATCAA GTACAATTCC 720

AACTCAGGCT TTGTCCGTGA TGACAACATC CGACTAACCC CACAGGCCTT CAGCCATTTC 780

ACATTTGAGC GTTCTGGTCA TCAGCTGATT GTAGTGGACA TCCAGGGTGT GGGTGACCTT 840

TATACCGACC CACAGATCCA CACTGAGAAA GGCACTGACT TTGGAGATGG TAACCTTGGT 900

GTCCGGGGAA TGGCTCTCTT CTTCTACTCT CATGCCTGCA ACCGGATTTG TCAGAGCATG 960

GGCCTTACGC CCTTTGACCT CGATAACATG GGCCCTAGAG ACCATGACCG CATGGACAAT 1020

CACCGGGACT CTGAGAATAG TGGGGACAGT GGGTATCCAA GCGAGAAGCG AAGTGACCTG 1080

GATGATCCTG AGCCCCGAGA ACACGGCCAC TCCAACGGCA ACCGAAGGCA TGAATCTGAC 1140

GAGGATAGCC TGGGCAGCTC TGGACGGGTC TGTGTGGAGA CGTGGAACCT GCTCAATCCC 1200

TCCCGCCTGC ACCTGCCGAG GCCCTCGGCC GTGGCCCTAG AAGTGCAGAG GCTAAATGCC 1260

CTGGACCTTG GAAGGAAAAT CGGGAAGTCT GTTTTGGGGA AGGTCCATTT GGCCATGGTG 1320

CGATACCACG AGGGCGGGCG CTTCTGCGAG AAGGATGAGG AGTGGGATCG AGAGTCAGCC 1380

ATCTTCCATC TGGAGCATGC AGCTGACCTG GGAGAACTGG AGGCCATCGT GGGCCTAGGC 1440

CTCATGTACT CTCAGCTGCC CCACCACATC CTGGCTGATG TCTCTCTGAA GGAGACAGAG 1500

GAGAACAAGA CAAAAGGCTT TGATTACTTA CTGAAGGCGG CAGAAGCTGG TGACAGGCAT 1560

TCCATGATTT TAGTGGCCCG AGCTTTTGAC ACTGGCCTGA ACCTCAGCCC AGACAGGTGT 1620

CAAGACTGGT CGGAAGCCTT GCACTGGTAC AACACAGCCC TGGAGACAAC AGACTGCGAT 1680

GAAGGCGGGG AGTACGATGG GATACAGGAC GAGCCCCAGT ACGCACTGCT GGCCAGGGAG 1740

GCGGAGATGC TGCTCACCGG GGGATTTGGA CTGGACAAGA ACCCCCAAAG ATCAGAGATG 1800

GAGTCTCATG TAGGCCAGTG GACCTCAAAC TCACTGTGTA GCTGA 1845

Domain Profile

S: 2     sesWsqqetivlikvlskeekkGalRkalrvkkgslflvkeelkevvryvakiykeqkvl  61

         +++W+++e  vlik++s+++++Ga+R+++r+kk+s+fl+++++k++++yvak+y+e++++

Q: 120   TGEWLKDE--VLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDR  177

         789*****..**************************************************

S: 62    hlylrdverqraaqklvtefnqvkPkkivyiprlievfLkdrtsknqlltvEeyllGefv  121

          +y++dv+ q++a+ +++++n++kP+k+v+i+++++++Lkdr+++ +l+++E+y++G+++

Q: 178   SVYFEDVQLQMEAKLWGEDYNRHKPPKQVDIMQMCIIELKDRPGQ-PLFHLEHYIEGKYI  236

         *********************************************.**************

S: 122   KynnnngdeikrenkleelmlafsHftyeytngellVvDlqGvgeklTDpqiatksrgtk  181

         Kyn+n+g  ++r+++++ +++afsHft+e+++++l+VvD+qGvg+ +TDpqi+t ++gt+

Q: 237   KYNSNSG--FVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHT-EKGTD  293

         *******..*********************************************.*****

S: 182   fgkanlgeraiknFfakhhCNeiC  205

         fg++nlg+r+++ Ff++h+CN+iC

Q: 294   FGDGNLGVRGMALFFYSHACNRIC  317

         ************************

Domain Sequence
(FASTA)

TGEWLKDEVL IKMASQPFGR GAMRECFRTK KLSNFLHAQQ WKGASNYVAK RYIEPVDRSV 60

YFEDVQLQME AKLWGEDYNR HKPPKQVDIM QMCIIELKDR PGQPLFHLEH YIEGKYIKYN 120

SNSGFVRDDN IRLTPQAFSH FTFERSGHQL IVVDIQGVGD LYTDPQIHTE KGTDFGDGNL 180

GVRGMALFFY SHACNRIC 198

Keyword

ATP-binding; Calcium; Calmodulin-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005524	; F:ATP binding
GO:0005509	; F:calcium ion binding
GO:0005516	; F:calmodulin binding
GO:0004686	; F:elongation factor-2 kinase activity
GO:0008135	; F:translation factor activity, nucleic acid binding
GO:0046777	; P:protein autophosphorylation
GO:0031952	; P:regulation of protein autophosphorylation
GO:0006414	; P:translational elongation

KEGG

mmu:13631

;

InterPros

IPR017400	; Elongation_factor_2_kinase.
IPR011009	; Kinase-like_dom.
IPR004166	; MHCK_EF2_kinase.
IPR006597	; Sel1-like.
IPR011990	; TPR-like_helical.

Pfam

PF02816	; Alpha_kinase; 1.
PF08238	; Sel1; 4.

SMARTs

SM00811

; Alpha_kinase; 1.

Prosites

PS51158

; ALPHA_KINASE; 1.

Prints

Created Date

20-Feb-2013