EKS-MUM-00251

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00251

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	343.0	2.3E-102	34	285	252

Status

Reviewed

Ensembl Protein

ENSMUSP00000039517

UniProt Accession

Q5RJI5; A7LH90; A7LH91; B7SRN7; Q699J6;

Protein Name

Serine/threonine-protein kinase BRSK1

Protein Synonyms/Alias

Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B;

Gene Name

Brsk1

Gene Synonyms/Alias

Brsk1; Gm1100, Sadb;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000035390	ENSMUSP00000039517	ENSMUST00000048248
ENSMUSG00000035390	ENSMUSP00000113448	ENSMUST00000120836

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase that plays a key role inpolarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

Protein Length

778

Protein Sequence
(FASTA)

MSSGSKEGGG GSPAYHLPHP HPHPPQHAQY VGPYRLEKTL GKGQTGLVKL GVHCITGQKV 60

AVKIVNREKL SESVLMKVER EIAILKLIEH PHVLKLHDVY ENKKYLYLVL EHVSGGELFD 120

YLVKKGRLTP KEARKFFRQI VSALDFCHSY SICHRDLKPE NLLLDEKNNI RIADFGMASL 180

QVGDSLLETS CGSPHYACPE VIKGEKYDGR RADMWSCGVI LFALLVGALP FDDDNLRQLL 240

EKVKRGVFHM PHFIPPDCQS LLRGMIEVEP EKRLSLEQIQ KHPWYLGGKH EPDPCLEPAP 300

GRRVAMRSLP SNGELDPDVL ESMASLGCFR DRERLHRELR SEEENQEKMI YYLLLDRKER 360

YPSCEDQDLP PRNDVDPPRK RVDSPMLSRH GKRRPERKSM EVLSITDAGS GGSPVPTRRA 420

LEMAQHSQRS RSVSGASTGL SSSPLSSPRS PVFSFSPEPG AGDEARGGGS PTSKTQTLPS 480

RGPRGGGAGE QPPPPSARST PLPGPPGSPR SSGGTPLHSP LHTPRASPTG TPGTTPPPSP 540

GGGVGGAAWR SRLNSIRNSF LGSPRFHRRK MQVPTAEEMS SLTPESSPEL AKRSWFGNFI 600

SLDKEEQIFL VLKDKPLSSI KADIVHAFLS IPSLSHSVLS QTSFRAEYKA SGGPSVFQKP 660

VRFQVDISSS EGPEPSPRRD GSSGGGIYSV TFTLISGPSR RFKRVVETIQ AQLLSTHDQP 720

SVQALADEKN GAQTRPAGTP PRSLQPPPGR SDPDLSSSPR RGPPKDKKLL ATNGTPLP 778

Nucleotide Sequence
(FASTA)

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 60

CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGCCTGGA GAAGACGCTG 120

GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGAGTCCACT GCATCACGGG TCAGAAGGTC 180

GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCGG TGCTGATGAA GGTGGAGAGG 240

GAAATCGCCA TCCTGAAGCT CATTGAACAC CCGCACGTGC TCAAGCTCCA CGACGTCTAC 300

GAGAACAAGA AATATTTATA CTTGGTTCTT GAGCACGTTT CTGGTGGTGA GCTGTTCGAC 360

TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GGAAGTTCTT CCGCCAGATC 420

GTGTCAGCAC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGAGACTT GAAGCCAGAG 480

AACCTGCTGT TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGTAT GGCGTCCCTG 540

CAAGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTACGC ATGTCCAGAG 600

GTGATCAAGG GGGAAAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 660

CTATTTGCCC TGCTTGTGGG GGCACTGCCC TTCGATGACG ACAACCTGCG CCAGCTACTG 720

GAGAAGGTGA AACGTGGGGT CTTCCACATG CCTCACTTCA TCCCTCCAGA CTGCCAGAGC 780

CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 840

AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 900

GGCCGCAGAG TAGCCATGCG TAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 960

GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTGCACAG AGAACTGCGA 1020

AGCGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 1080

TATCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGTAAG 1140

CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 1200

GAAGTCCTCA GTATCACAGA TGCTGGGAGT GGTGGCTCCC CAGTGCCTAC CAGACGGGCC 1260

TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCCTC CACTGGTCTG 1320

TCCTCCAGCC CTCTGAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 1380

GCTGGAGATG AGGCTAGAGG CGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 1440

CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CCCCCAGTGC CCGTTCCACG 1500

CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 1560

CTGCACACGC CTCGAGCCAG CCCTACGGGG ACCCCAGGAA CGACGCCACC TCCCAGCCCA 1620

GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGGCTCA ACTCCATCCG CAACAGCTTC 1680

CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCCGA GGAGATGTCC 1740

AGTTTGACAC CAGAATCCTC TCCTGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 1800

TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 1860

AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTACTGTCA 1920

CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGCGGCC CCTCCGTCTT CCAGAAGCCT 1980

GTCCGCTTTC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 2040

GGCAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTTACTC TCATCTCCGG TCCCAGCCGT 2100

CGGTTCAAAC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA CGACCAGCCC 2160

TCCGTGCAGG CCTTGGCAGA TGAGAAGAAC GGAGCCCAGA CCCGGCCTGC TGGGACCCCA 2220

CCCCGAAGCC TGCAGCCCCC ACCAGGCCGC TCTGACCCTG ATCTGAGTAG CTCTCCCCGC 2280

CGAGGACCCC CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGA 2337

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkklseeslekikrEieilkklkhpni  60

         y+l ktlG+g++g Vkl +h  t++kvavKi++++klse+ l k++rEi+ilk ++hp++

Q: 34    YRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHV  93

         99**********************************************************

S: 61    ikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgiv  120

         +kl++v+e+kk+lylvle++sggeLfdy+++kgrl+ keark+f+q+vsa++++hs +i+

Q: 94    LKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSIC  153

         ************************************************************

S: 121   HrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkvD  180

         HrDlKpeNllld+k+ni+iaDFG+a+l   ++llet+cGsp+Ya+Pe++kg+kY+g+++D

Q: 154   HRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRAD  213

         ************************************************************

S: 181   vWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpekR  240

         +Ws+Gv+L+al++g+lPfd++nl++llek+++g +++p+ + ++++sLlr +++v+pekR

Q: 214   MWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKR  273

         ************************************************************

S: 241   atieeilkhewl  252

         +++e+i+kh+w+

Q: 274   LSLEQIQKHPWY  285

         ***********5

Domain Sequence
(FASTA)

YRLEKTLGKG QTGLVKLGVH CITGQKVAVK IVNREKLSES VLMKVEREIA ILKLIEHPHV 60

LKLHDVYENK KYLYLVLEHV SGGELFDYLV KKGRLTPKEA RKFFRQIVSA LDFCHSYSIC 120

HRDLKPENLL LDEKNNIRIA DFGMASLQVG DSLLETSCGS PHYACPEVIK GEKYDGRRAD 180

MWSCGVILFA LLVGALPFDD DNLRQLLEKV KRGVFHMPHF IPPDCQSLLR GMIEVEPEKR 240

LSLEQIQKHP WY 252

Keyword

Alternative splicing; ATP-binding; Cell cycle; Cell junction; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; Kinase; Magnesium; Metal-binding; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Synapse; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Xenopus tropicalis"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0030054	; C:cell junction
GO:0005813	; C:centrosome
GO:0005634	; C:nucleus
GO:0005886	; C:plasma membrane
GO:0008021	; C:synaptic vesicle
GO:0005524	; F:ATP binding
GO:0043015	; F:gamma-tubulin binding
GO:0000287	; F:magnesium ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0050321	; F:tau-protein kinase activity
GO:0007409	; P:axonogenesis
GO:0051298	; P:centrosome duplication
GO:0030010	; P:establishment of cell polarity
GO:0031572	; P:G2/M transition DNA damage checkpoint
GO:0007269	; P:neurotransmitter secretion
GO:0009411	; P:response to UV

KEGG

mmu:381979

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR015940	; UBA/transl_elong_EF1B_N_euk.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50030	; UBA; 1.

Prints

Created Date

20-Feb-2013