EKS-MUM-00259

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00259

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	317.9	1.0E-94	11	263	253

Status

Reviewed

Ensembl Protein

ENSMUSP00000043806

UniProt Accession

Q61846; Q3TPU1; Q61804; Q6ZQH6;

Protein Name

Maternal embryonic leucine zipper kinase

Protein Synonyms/Alias

Protein kinase PK38; mPK38; Tyrosine-protein kinase MELK;

Gene Name

Melk

Gene Synonyms/Alias

Melk; Kiaa0175, Pk38;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000035683	ENSMUSP00000118359	ENSMUST00000125708
ENSMUSG00000035683	ENSMUSP00000043806	ENSMUST00000045607
ENSMUSG00000035683	ENSMUSP00000120242	ENSMUST00000137703

Organism

Mus musculus

Functional Description

Serine/threonine-protein kinase involved in variousprocesses such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

Protein Length

643

Protein Sequence
(FASTA)

MKDYDELLKY YELYETIGTG GFAKVKLACH VLTGEMVAIK IMDKNALGSD LPRVKTEIDA 60

LKSLRHQHIC QLYHVLETKN KIFMVLEYCP GGELFDYIIS QDRLSEEETR VVFRQILSAV 120

AYVHSQGYAH RDLKPENLLF DENHKLKLID FGLCAKPKGN KDYHLQTCCG SLAYAAPELI 180

QGKSYLGSEA DVWSMGILLY VLMCGFLPFD DDNVMALYKK IMRGKYEVPK WLSPSSILLL 240

QQMLQVDPKK RISMRNLLNH PWVMQDYSCP VEWQSKTPLT HLDEDCVTEL SVHHRSSRQT 300

MEDLISSWQY DHLTATYLLL LAKKARGKPA RLQLLSFSCG TASTTPKSKN LSLEDMSTSD 360

DNCVAGLIDY ELCEDKLLAP KTPQVTKHLA ESNHAASKSP APGVRRAVAN KLMDKENVCT 420

PKSSVKNEEQ FVFSEPKIPV SKNQYKREIP ASPTRFPTPA KARAQCLREA PVRTPGNSAG 480

ADTLTTGVIS PERRCRSMDV DLNQAHMEDT PKKKGTNVFG SLERGLDKVL TALTRNKKKG 540

SARDGPRKRK LHYNVTTTRL VNPDQLLSEI MAILPKKNVD FVQKGYTLKC QTQSDFGKVT 600

MQFELEVCQL QRPDVVGIRR QRLKGDAWVY KRLVEDILSG CKM 643

Nucleotide Sequence
(FASTA)

ATGAAAGATT ATGACGAACT CCTCAAATAC TATGAACTAT ATGAAACGAT TGGGACAGGT 60

GGCTTTGCAA AGGTCAAACT GGCCTGCCAT GTCCTCACTG GAGAGATGGT AGCTATAAAA 120

ATCATGGATA AGAATGCGCT AGGGAGTGAT TTGCCCCGAG TCAAAACTGA GATCGATGCG 180

CTGAAGAGTC TGAGACATCA GCACATATGT CAGCTCTACC ATGTGCTGGA GACAAAGAAC 240

AAAATATTCA TGGTTCTGGA GTACTGTCCA GGAGGAGAGC TGTTTGACTA CATAATCTCC 300

CAGGATCGCC TGTCGGAAGA GGAGACCCGG GTCGTCTTCC GTCAGATACT GTCTGCAGTT 360

GCGTATGTCC ACAGCCAGGG CTATGCCCAC AGGGACCTCA AACCAGAAAA TTTATTATTT 420

GATGAAAATC ATAAGCTAAA GCTGATTGAC TTTGGTCTTT GTGCAAAACC CAAGGGCAAC 480

AAGGACTACC ATCTGCAGAC GTGCTGTGGG AGCCTTGCTT ATGCAGCTCC TGAACTAATA 540

CAAGGGAAGT CGTACCTTGG ATCAGAGGCA GATGTTTGGA GCATGGGCAT CCTCCTGTAT 600

GTGCTCATGT GTGGATTTCT ACCATTTGAT GATGATAATG TCATGGCTTT GTACAAGAAG 660

ATAATGAGAG GGAAATACGA AGTTCCTAAG TGGCTCTCTC CCAGTAGCAT TCTGCTTCTC 720

CAGCAGATGT TGCAGGTGGA CCCAAAGAAA CGGATTTCTA TGAGAAATCT CCTGAACCAT 780

CCCTGGGTCA TGCAAGATTA CAGCTGTCCC GTGGAGTGGC AAAGCAAGAC TCCTTTGACT 840

CACCTCGATG AGGATTGCGT GACAGAGCTT TCTGTACATC ACCGCAGCAG CAGGCAGACA 900

ATGGAGGATT TAATTTCGTC GTGGCAGTAC GATCACCTCA CAGCCACCTA CCTTCTGCTT 960

CTAGCCAAGA AGGCCCGGGG GAAGCCGGCT CGTCTACAGC TCCTGTCCTT CTCTTGTGGA 1020

ACCGCCAGCA CCACTCCAAA GTCAAAGAAT CTGAGCCTGG AAGATATGAG CACAAGTGAT 1080

GATAACTGTG TGGCTGGATT GATAGACTAT GAATTGTGTG AAGATAAATT ATTAGCTCCC 1140

AAGACGCCAC AGGTTACCAA ACACTTGGCA GAATCAAATC ACGCAGCATC TAAATCACCA 1200

GCGCCAGGGG TACGCAGAGC AGTGGCAAAT AAATTAATGG ACAAAGAAAA TGTGTGCACT 1260

CCCAAGTCTT CTGTGAAGAA TGAAGAGCAG TTTGTATTTT CTGAGCCGAA GATTCCAGTT 1320

AGTAAGAACC AGTATAAGAG AGAAATACCC GCCTCACCAA CCCGTTTCCC AACACCTGCA 1380

AAAGCTAGAG CCCAGTGCCT GAGAGAAGCC CCGGTTAGAA CACCAGGGAA TTCCGCAGGA 1440

GCAGACACAC TAACGACAGG TGTCATTAGC CCCGAGAGGA GGTGCCGTTC AATGGACGTG 1500

GATCTCAACC AGGCACACAT GGAGGATACC CCGAAAAAGA AAGGAACCAA TGTGTTTGGG 1560

AGCCTTGAGA GAGGACTGGA TAAGGTTCTC ACTGCGCTCA CAAGGAACAA GAAGAAGGGC 1620

TCTGCCAGAG ATGGACCAAG AAAGCGAAAG CTGCACTACA ATGTGACTAC AACTCGCCTG 1680

GTGAACCCCG ACCAGCTCCT GAGCGAAATC ATGGCTATTC TTCCAAAGAA GAACGTGGAC 1740

TTCGTACAGA AAGGTTACAC TCTAAAGTGT CAAACGCAGT CTGATTTTGG CAAAGTGACA 1800

ATGCAGTTTG AACTGGAAGT GTGCCAGCTG CAGAGACCTG ACGTGGTAGG CATCCGGAGA 1860

CAGCGGCTGA AGGGTGATGC CTGGGTTTAC AAGAGATTAG TGGAAGATAT CTTGTCTGGC 1920

TGCAAGATGT GA 1932

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkklseeslekikrEieilkklkhpni  60

         y+l +t+G+g f+kVkla+h +t+e+va+Ki++k++l +  l ++k+Ei++lk+l+h++i

Q: 11    YELYETIGTGGFAKVKLACHVLTGEMVAIKIMDKNALGS-DLPRVKTEIDALKSLRHQHI  69

         999*******************************88875.589*****************

S: 61    ikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgiv  120

         ++l++v+etk+k+++vley+ ggeLfdyi ++ rl+e+e+r +f+q++sav Y+hs+g +

Q: 70    CQLYHVLETKNKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQILSAVAYVHSQGYA  129

         ************************************************************

S: 121   HrDlKpeNllldkkenikiaDFGlanlfkeg..klletfcGsppYaaPellkgkkYegpk  178

         HrDlKpeNll+d+++++k++DFGl+++ k +   +l+t+cGs++YaaPel++gk+Y g++

Q: 130   HRDLKPENLLFDENHKLKLIDFGLCAKPKGNkdYHLQTCCGSLAYAAPELIQGKSYLGSE  189

         *************************9665443389*************************

S: 179   vDvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpe  238

         +DvWs+G++Ly+l++g lPfd++n+ +l++ki++gk+e+pk+ls++ + Ll+++L+vdp+

Q: 190   ADVWSMGILLYVLMCGFLPFDDDNVMALYKKIMRGKYEVPKWLSPSSILLLQQMLQVDPK  249

         ************************************************************

S: 239   kRatieeilkhewl  252

         kR++++++l+h+w+

Q: 250   KRISMRNLLNHPWV  263

         *************7

Domain Sequence
(FASTA)

YELYETIGTG GFAKVKLACH VLTGEMVAIK IMDKNALGSD LPRVKTEIDA LKSLRHQHIC 60

QLYHVLETKN KIFMVLEYCP GGELFDYIIS QDRLSEEETR VVFRQILSAV AYVHSQGYAH 120

RDLKPENLLF DENHKLKLID FGLCAKPKGN KDYHLQTCCG SLAYAAPELI QGKSYLGSEA 180

DVWSMGILLY VLMCGFLPFD DDNVMALYKK IMRGKYEVPK WLSPSSILLL QQMLQVDPKK 240

RISMRNLLNH PWV 253

Keyword

Apoptosis; ATP-binding; Calcium; Cell cycle; Cell membrane; Complete proteome; Kinase; Lipid-binding; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005938	; C:cell cortex
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0005509	; F:calcium ion binding
GO:0008289	; F:lipid binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0006915	; P:apoptotic process
GO:0007049	; P:cell cycle
GO:0030097	; P:hemopoiesis
GO:0061351	; P:neural precursor cell proliferation
GO:0043065	; P:positive regulation of apoptotic process
GO:0046777	; P:protein autophosphorylation

KEGG

mmu:17279

;

InterPros

IPR001772	; Kinase-assoc_KA1.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF02149	; KA1; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50032	; KA1; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013