EKS-MUM-00459

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-MUM-00459

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	361.9	4.2E-108	208	480	273

Status

Reviewed

Ensembl Protein

ENSMUSP00000038754

UniProt Accession

Q66JT0; Q4U4S4; Q7TPV9;

Protein Name

Wee1-like protein kinase 2

Protein Synonyms/Alias

Wee1-like protein kinase 1B; Wee1B kinase; mWee1B;

Gene Name

Wee2

Gene Synonyms/Alias

Wee2; Wee1b;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000037159	ENSMUSP00000038754	ENSMUST00000038907
ENSMUSG00000037159	ENSMUSP00000122678	ENSMUST00000141490

Organism

Mus musculus

Functional Description

Oocyte-specific protein tyrosine kinase thatphosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.

Protein Length

555

Protein Sequence
(FASTA)

MADTETDQGL NKKLSFSFCE EDTESEGQMT AQDIGGAQSQ KPGREESEEL EGPVPPTRDE 60

LHTSLSRDKE SPGPDLWKSV SGPPKCPETP VLHSKSKLPV PTNFSTPKNS LGQPEISLLE 120

KLSSRSSRHM RLTPASLMDE KTSLSLVNIN PFTPETYRKL LLQSKGKRKT RDYLEETGEE 180

ESKSVQWLPA KRSILQETNM ASRYEKEFFE IEKIGVGEFG TVYKCIKRLD GCIYAIKRSA 240

KSFSGLSNEL DLHEVYAHAV LGHHPHVVRY YSSWIEDDHV VIQNEYCNGG SLQAAISENT 300

ASNNHFQEPK LKDILLQISL GLKYIHNSGM VHLDIKPSNI FICHKMQCDS PVGPEEAESE 360

ADWFLNASVM YKIGDLGHAT SISKPKVEEG DTRFLANEIL QENYQHLPKA DIFALGLTIA 420

VAAGAESLPI NGDMWHHIRK GNFPEISQEL SDDFYGLLKN MIHPAPKERP SAAALARSRI 480

LWPFLEKTDE LQKQLNLEKS KTATLKRELK KARHIQTPQR EVHHCYYQIC KGSRHLLVGG 540

RRKAPSSFTR GTSSV 555

Nucleotide Sequence
(FASTA)

ATGGCCGACA CAGAGACTGA CCAGGGACTG AACAAGAAAT TAAGCTTCTC CTTTTGTGAA 60

GAAGACACTG AGAGTGAAGG GCAGATGACT GCACAAGACA TCGGAGGGGC CCAGAGCCAA 120

AAGCCTGGGA GGGAAGAAAG TGAGGAGTTG GAGGGTCCCG TGCCTCCTAC CAGAGATGAA 180

CTTCATACTT CTCTGAGCAG AGACAAGGAA AGTCCAGGTC CTGATCTTTG GAAATCAGTT 240

TCAGGCCCTC CCAAATGCCC AGAGACACCA GTATTACACA GCAAGAGCAA GCTGCCAGTC 300

CCTACCAACT TCTCCACTCC CAAAAATTCG CTGGGTCAGC CAGAGATTTC TCTGCTAGAG 360

AAGCTCTCCT CCCGAAGCTC TAGACATATG AGGCTCACAC CTGCTTCCCT TATGGATGAG 420

AAGACCTCTT TGTCTCTGGT CAATATCAAC CCATTCACTC CGGAGACCTA CAGAAAATTA 480

CTCCTTCAAT CAAAGGGCAA GAGGAAGACC AGAGACTATC TTGAGGAAAC TGGAGAAGAG 540

GAAAGCAAAT CAGTACAGTG GCTGCCTGCT AAGAGAAGTA TTCTACAAGA AACTAATATG 600

GCTTCCCGCT ATGAAAAAGA ATTCTTCGAG ATAGAAAAAA TCGGGGTTGG AGAATTTGGG 660

ACAGTCTACA AGTGCATTAA GAGGCTGGAT GGATGCATTT ATGCAATAAA GCGCTCTGCA 720

AAGTCCTTTT CAGGATTATC AAATGAATTG GATTTGCATG AAGTTTATGC TCATGCAGTG 780

CTTGGCCATC ATCCCCATGT GGTACGCTAC TACTCATCAT GGATAGAAGA TGATCATGTG 840

GTCATTCAAA ATGAATACTG TAATGGTGGG AGCCTGCAAG CTGCTATATC TGAAAACACT 900

GCGTCCAATA ATCACTTCCA AGAGCCCAAA CTCAAAGACA TCCTTCTGCA GATTTCCTTG 960

GGACTTAAGT ACATCCACAA TTCTGGCATG GTACACCTGG ACATCAAACC CAGTAACATC 1020

TTCATTTGTC ATAAGATGCA GTGTGACTCT CCTGTAGGCC CAGAAGAGGC TGAAAGTGAA 1080

GCTGACTGGT TTCTCAATGC CAGTGTGATG TATAAAATTG GTGACCTGGG CCACGCGACA 1140

TCAATCAGCA AACCTAAAGT GGAAGAAGGA GATACTCGTT TCCTGGCTAA TGAGATCTTG 1200

CAAGAGAATT ACCAACACCT CCCCAAAGCA GACATATTTG CCTTAGGATT AACAATCGCA 1260

GTGGCTGCAG GAGCAGAGTC TTTACCCATC AATGGTGACA TGTGGCATCA TATCCGCAAA 1320

GGGAACTTTC CAGAGATTTC CCAAGAGCTC TCGGATGACT TTTATGGTCT ACTCAAGAAC 1380

ATGATCCACC CAGCTCCAAA GGAGAGACCA TCTGCAGCAG CTCTGGCCAG AAGTCGAATT 1440

CTCTGGCCTT TCTTGGAGAA GACTGATGAA CTCCAGAAAC AACTGAATTT GGAGAAATCC 1500

AAGACAGCCA CACTGAAAAG GGAACTGAAA AAAGCTCGGC ATATCCAGAC CCCTCAGAGA 1560

GAAGTCCACC ATTGTTATTA TCAGATCTGC AAAGGCTCAA GACACCTCCT GGTGGGAGGA 1620

AGGAGGAAGG CGCCCTCAAG CTTCACCCGT GGGACATCCT CCGTGTAG 1668

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikkskkplkglskekkalrEvtahavlgqhen  60

         F e+ekiG+Gefg+v+k++kr+dg++yAik+s+k+++gls+e + l+Ev+ahavlg+h++

Q: 208   FFEIEKIGVGEFGTVYKCIKRLDGCIYAIKRSAKSFSGLSNELD-LHEVYAHAVLGHHPH  266

         889***************************************95.***************

S: 61    vvryisaWeeddslyiqtElCeggsLeaalqeceakkkaleeaelkkilldlllglrylH  120

         vvry+s+W+edd+++iq+E+C+ggsL+aa++e++a++++++e +lk+ill+++lgl+y+H

Q: 267   VVRYYSSWIEDDHVVIQNEYCNGGSLQAAISENTASNNHFQEPKLKDILLQISLGLKYIH  326

         ************************************************************

S: 121   ssslvHlDikpenifi.......................tkevtlklgDlGlvtelnsed  157

         +s +vHlDikp+nifi                       +++v++k+gDlG++t++   +

Q: 327   NSGMVHLDIKPSNIFIchkmqcdspvgpeeaeseadwflNASVMYKIGDLGHATSI---S  383

         ********************************************************...*

S: 158   kskveEGDsrylakEilqeeyskl.kaDiFslgltilevaanvelPsnGeaWhklRkgkl  216

         k+kveEGD+r+la+Eilqe+y++l kaDiF+lglti+++a++++lP nG++Wh++Rkg++

Q: 384   KPKVEEGDTRFLANEILQENYQHLpKADIFALGLTIAVAAGAESLPINGDMWHHIRKGNF  443

         ************************************************************

S: 217   pdeilkelsedlrellklmlepdperRptaeellkhev  254

         p ei +els+d+  llk+m++p+p++Rp+a++l+++++

Q: 444   P-EISQELSDDFYGLLKNMIHPAPKERPSAAALARSRI  480

         *.*******************************99876

Domain Sequence
(FASTA)

FFEIEKIGVG EFGTVYKCIK RLDGCIYAIK RSAKSFSGLS NELDLHEVYA HAVLGHHPHV 60

VRYYSSWIED DHVVIQNEYC NGGSLQAAIS ENTASNNHFQ EPKLKDILLQ ISLGLKYIHN 120

SGMVHLDIKP SNIFICHKMQ CDSPVGPEEA ESEADWFLNA SVMYKIGDLG HATSISKPKV 180

EEGDTRFLAN EILQENYQHL PKADIFALGL TIAVAAGAES LPINGDMWHH IRKGNFPEIS 240

QELSDDFYGL LKNMIHPAPK ERPSAAALAR SRI 273

Keyword

ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Magnesium; Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Equus caballus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Ochotona princeps"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>

Gene Ontology

GO:0005813	; C:centrosome
GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0000287	; F:magnesium ion binding
GO:0004715	; F:non-membrane spanning protein tyrosine kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007343	; P:egg activation
GO:0007143	; P:female meiosis
GO:0035038	; P:female pronucleus assembly
GO:0007137	; P:meiotic metaphase II
GO:0007128	; P:meiotic prophase I
GO:0007067	; P:mitosis
GO:0060283	; P:negative regulation of oocyte development
GO:0060631	; P:regulation of meiosis I

KEGG

mmu:381759

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR017164	; Wee1-like_protein_kinase.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013