EPS-MUM-00022

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-MUM-00022

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	239.1	1.7E-70	94	344	251

Status

Reviewed

Ensembl Protein

ENSMUSP00000041395

UniProt Accession

Q8BXN7;

Protein Name

Protein phosphatase 1K, mitochondrial

Protein Synonyms/Alias

Protein phosphatase 2C isoform kappa; PP2C-kappa;

Gene Name

Ppm1k

Gene Synonyms/Alias

Ppm1k; Pp2cm;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
ENSMUSG00000037826	ENSMUSP00000041395	ENSMUST00000042766

Organism

Mus musculus

Functional Description

Regulates the mitochondrial permeability transition poreand is essential for cellular survival and development.

Protein Length

372

Protein Sequence
(FASTA)

MLSAAFITLL RSGGNQVKKR VLLSSILLQD HRQATPACYF STSEARCSRF DPDGSGQPAT 60

WDNFGIWDNR IDEPILLPPS IKYGKPIPKI SLENVGCASL IGKRKENEDR FGFAQLTEEV 120

LYFAVYDGHG GPAAADFCHT HMEKCVMDLL PREKDLETVL TLAFLEIDKA FASYAHLSAD 180

ASLLTSGTTA TVALLRDGVE LVVASVGDSR ALLCRKGKPM KLTTDHTPER KDEKERIKKF 240

GGFVAWNSLG QPHVNGRLAM TRSIGDLDLK ASGVIAEPET TRIKLYHADD SFLVLTTDGI 300

NFMVNSQEIC DFVNQCHDPK EAAHSVTEQA IQYGTEDNST AVVVPFGAWG KYKNSEITFS 360

FSRSFASSGR WA 372

Nucleotide Sequence
(FASTA)

ATGTTATCAG CGGCCTTCAT TACTTTGTTG AGAAGTGGTG GGAATCAGGT GAAGAAGAGA 60

GTGTTGTTAA GCTCCATCCT CCTGCAGGAC CACAGGCAGG CGACTCCTGC CTGCTACTTC 120

TCCACTTCAG AGGCCAGGTG TTCTCGGTTT GATCCAGATG GAAGTGGGCA GCCAGCCACT 180

TGGGACAATT TTGGGATCTG GGATAACCGC ATTGATGAGC CAATTCTGCT GCCACCTAGC 240

ATCAAGTACG GCAAACCAAT TCCCAAAATT AGCCTGGAGA ACGTGGGCTG TGCCTCTCTC 300

ATTGGCAAAC GGAAAGAAAA CGAAGATCGA TTTGGGTTTG CACAGCTGAC AGAAGAGGTG 360

CTATACTTCG CAGTCTATGA TGGACATGGG GGCCCTGCAG CCGCTGACTT CTGTCACACA 420

CACATGGAGA AATGTGTTAT GGATTTGCTT CCTCGGGAGA AAGACTTGGA AACTGTCCTG 480

ACCTTGGCCT TTCTAGAAAT AGATAAAGCC TTCGCGAGTT ATGCCCACCT GTCTGCAGAT 540

GCAAGCCTCC TGACCTCTGG GACTACTGCA ACAGTAGCCC TGTTGAGAGA TGGTGTTGAA 600

CTGGTGGTAG CCAGTGTTGG AGACAGCCGG GCTCTTTTGT GTAGAAAAGG AAAACCCATG 660

AAGCTGACCA CTGACCATAC CCCAGAAAGA AAAGATGAGA AAGAAAGGAT CAAGAAATTC 720

GGTGGGTTTG TAGCTTGGAA TAGTTTGGGA CAGCCCCATG TAAATGGCAG ACTTGCAATG 780

ACAAGGAGTA TCGGAGATTT GGATCTTAAA GCCAGTGGGG TAATTGCAGA ACCTGAGACA 840

ACAAGGATTA AGCTCTACCA TGCTGACGAC AGTTTCCTGG TCCTTACCAC AGATGGGATT 900

AACTTCATGG TGAATAGTCA AGAGATCTGC GACTTTGTCA ACCAGTGCCA CGATCCTAAA 960

GAAGCAGCTC ATTCGGTGAC TGAGCAGGCA ATACAGTACG GTACCGAAGA CAACAGCACT 1020

GCAGTAGTGG TGCCCTTTGG TGCTTGGGGA AAATACAAGA ACTCTGAAAT TACCTTCTCA 1080

TTCAGCAGAA GCTTTGCCTC CAGTGGGAGA TGGGCCTGA 1119

Domain Profile

S: 2     lvgvvsvngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheil  61

         +vg++s+ g+r+e ed++    +f++ +e+   + +f+vyDGhgg  +a+fc++++++++

Q: 94    NVGCASLIGKRKENEDRF----GFAQLTEE---VLYFAVYDGHGGPAAADFCHTHMEKCV  146

         5899**************....67887666...78*************************

S: 62    aeelesseseerkvkkalakaFlstdeelkvleeksldidk....sGstavvalikds.k  116

            l     +e++++++l+ aFl++d+++   ++++l+ d+    sG+ta+val++d  +

Q: 147   MDLLP----REKDLETVLTLAFLEIDKAF--ASYAHLSADAslltSGTTATVALLRDGvE  200

         ***87....********************..77777777777799************99*

S: 117   lyvanvGdSravLcrngkavelssdHkpsredEleRieeaggsvilkd.ga.RvlGvLav  174

         l+va vGdSra+Lcr+gk+++l++dH+p+r+dE+eRi++ gg v+ ++ g+ +v+G La+

Q: 201   LVVASVGDSRALLCRKGKPMKLTTDHTPERKDEKERIKKFGGFVAWNSlGQpHVNGRLAM  260

         ********************************************98889999********

S: 175   sRalGdlylk.pyvtpePevtshrltsede.fliLasDGlwdvvsneeavslvrdvlekk  232

         +R++Gdl+lk + v++ePe+t ++l + d+ fl+L +DG+  +v+++e++++v+++++

Q: 261   TRSIGDLDLKaSGVIAEPETTRIKLYHADDsFLVLTTDGINFMVNSQEICDFVNQCHD--  318

         **********556************999999***************************..

S: 233   eaeqeaakkLvklalargskdnvsaivv  260

           ++eaa++++++a+++g++dn +a+vv

Q: 319   --PKEAAHSVTEQAIQYGTEDNSTAVVV  344

         ..***********************997

Domain Sequence
(FASTA)

NVGCASLIGK RKENEDRFGF AQLTEEVLYF AVYDGHGGPA AADFCHTHME KCVMDLLPRE 60

KDLETVLTLA FLEIDKAFAS YAHLSADASL LTSGTTATVA LLRDGVELVV ASVGDSRALL 120

CRKGKPMKLT TDHTPERKDE KERIKKFGGF VAWNSLGQPH VNGRLAMTRS IGDLDLKASG 180

VIAEPETTRI KLYHADDSFL VLTTDGINFM VNSQEICDFV NQCHDPKEAA HSVTEQAIQY 240

GTEDNSTAVV V 251

Keyword

Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Mitochondrion; Protein phosphatase; Reference proteome; Transit peptide.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005759	; C:mitochondrial matrix
GO:0005739	; C:mitochondrion
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0006470	; P:protein dephosphorylation

KEGG

mmu:243382

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013