| Tag | Content |
|---|
| EKPD ID | EKS-MUM-00407 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| TK/Src | 386.2 | 1.5E-115 | 192 | 441 | 250 |
|
| Status | Reviewed |
| Ensembl Protein | ENSMUSP00000016511 |
| UniProt Accession | Q64434; |
| Protein Name | Protein-tyrosine kinase 6 |
| Protein Synonyms/Alias | SRC-related intestinal kinase; |
| Gene Name | Ptk6 |
| Gene Synonyms/Alias | Ptk6; Sik; |
| Ensembl Information | |
| Organism | Mus musculus |
| Functional Description | Non-receptor tyrosine-protein kinase implicated in theregulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage- independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways. |
| Protein Length | 451 |
Protein Sequence
(FASTA) | | MVSWDKAHLG PKYVGLWDFK ARTDEELSFQ AGDLLHVTKK EELWWWATLL DAEGKALAEG 60 | | YVPHNYLAEK ETVESEPWFF GCISRSEAMH RLQAEDNSKG AFLIRVSQKP GADYVLSVRD 120 | | AQAVRHYRIW KNNEGRLHLN EAVSFSNLSE LVDYHKTQSL SHGLQLSMPC WKHKTEPLPH 180 | | WDDWERPREE FTLCKKLGAG YFGEVFEALW KGQVHVAVKV ISRDNLLHQH TFQAEIQAMK 240 | | KLRHKHILSL YAVATAGDPV YIITELMPKG NLLQLLRDSD EKALPILELV DFASQVAEGM 300 | | CYLESQNYIH RDLAARNVLV TENNLCKVGD FGLARLVKED IYLSHEHNVP YKWTAPEALS 360 | | RGHYSIKSDV WSFGVLLHEI FSRGQMPYPG MSNHETFLRV DAGYRMPCPL ECPPNIHKLM 420 | | LSCWSRDPKQ RPCFKDLCEK LTGITRYENL V 451 |
|
Nucleotide Sequence
(FASTA) | | ATGGTGTCTT GGGACAAGGC TCACCTGGGT CCTAAGTATG TGGGCCTCTG GGACTTCAAG 60 | | GCACGGACAG ATGAGGAGCT GAGCTTTCAG GCAGGAGACC TCCTCCATGT TACCAAGAAG 120 | | GAGGAACTGT GGTGGTGGGC CACCCTGCTG GATGCAGAAG GCAAGGCCTT GGCTGAGGGC 180 | | TATGTGCCTC ACAACTACCT GGCTGAGAAG GAAACTGTGG AGTCTGAACC GTGGTTCTTT 240 | | GGTTGCATCT CCCGCTCAGA GGCCATGCAC AGGCTGCAGG CTGAGGACAA CTCGAAGGGT 300 | | GCCTTCCTGA TCAGAGTCAG CCAGAAGCCA GGAGCAGACT ATGTCCTCTC TGTCCGGGAT 360 | | GCTCAGGCCG TGCGACATTA CAGGATCTGG AAGAACAACG AGGGCCGGCT GCACCTGAAT 420 | | GAGGCGGTAT CCTTCTCCAA TCTGTCTGAG CTTGTGGACT ACCATAAGAC CCAGAGCCTG 480 | | TCTCATGGCC TACAGCTGTC CATGCCCTGC TGGAAGCACA AAACTGAGCC CTTGCCCCAC 540 | | TGGGATGACT GGGAGAGGCC GAGGGAGGAG TTCACACTCT GTAAGAAGCT GGGGGCCGGC 600 | | TACTTTGGGG AGGTCTTTGA AGCGCTCTGG AAAGGCCAGG TCCATGTGGC TGTGAAGGTG 660 | | ATCTCTAGAG ACAATCTCCT GCACCAGCAC ACCTTCCAGG CTGAGATTCA GGCCATGAAG 720 | | AAGCTGCGGC ACAAGCACAT CCTGTCACTG TACGCTGTGG CGACTGCAGG GGACCCGGTC 780 | | TACATCATCA CGGAGCTCAT GCCCAAGGGG AACCTGCTGC AGCTACTGCG TGACTCTGAT 840 | | GAGAAAGCCC TGCCTATTTT GGAGCTGGTG GACTTTGCAT CACAGGTTGC TGAGGGCATG 900 | | TGCTACCTGG AATCTCAGAA TTACATCCAC CGTGACCTGG CTGCAAGGAA CGTTCTTGTT 960 | | ACAGAGAACA ATCTCTGCAA AGTGGGGGAC TTTGGGCTTG CCAGGCTTGT CAAGGAGGAC 1020 | | ATCTACCTTT CCCATGAGCA CAATGTCCCC TACAAATGGA CAGCACCTGA GGCACTTTCC 1080 | | CGAGGGCATT ACTCCATCAA GTCTGATGTC TGGTCTTTTG GAGTTCTTCT TCATGAAATT 1140 | | TTCAGCAGGG GGCAGATGCC CTACCCAGGC ATGTCCAATC ATGAAACCTT CCTGAGGGTG 1200 | | GATGCCGGCT ACCGCATGCC CTGCCCTCTG GAGTGCCCAC CCAACATACA CAAGCTGATG 1260 | | CTCAGCTGCT GGAGCAGAGA CCCCAAGCAG AGACCTTGCT TCAAAGACCT GTGTGAGAAA 1320 | | CTCACAGGTA TCACCAGGTA TGAGAACCTG GTCTGA 1356 |
|
| Domain Profile | S: 2 klvkklGeGqfGevwlgkwkgsvkvavktlkegt.lspeafleeaqilkklrheklvkly 60 | +l+kklG+G+fGev++++wkg+v+vavk++++++ l++++f++e+q++kklrh+++++ly | Q: 192 TLCKKLGAGYFGEVFEALWKGQVHVAVKVISRDNlLHQHTFQAEIQAMKKLRHKHILSLY 251 | 799******************************98999********************** |
| S: 61 avvseeePiyivtelmakGslldflkeeegkklklpklvdlaaqvaeGmayleeknlihr 120 | av+++++P+yi+telm kG+ll++l+++++k+l++ +lvd+a+qvaeGm+yle++n+ihr | Q: 252 AVATAGDPVYIITELMPKGNLLQLLRDSDEKALPILELVDFASQVAEGMCYLESQNYIHR 311 | ************************************************************ |
| S: 121 dlaarnvlvgeslvvkvadfGlarlieddeytakegaklPikWtaPeaiklgkftiksdv 180 | dlaarnvlv+e++++kv+dfGlarl+++d+y ++e +++P+kWtaPea++ g+++iksdv | Q: 312 DLAARNVLVTENNLCKVGDFGLARLVKEDIYLSHE-HNVPYKWTAPEALSRGHYSIKSDV 370 | ********************************987.99********************** |
| S: 181 WsfGillteivtkGkvPypGmtneevleqvergyrlprpeecpeelyelllecwkkdpee 240 | WsfG+ll+ei+++G++PypGm+n+e++ +v++gyr+p+p ecp ++++l+l+cw++dp++ | Q: 371 WSFGVLLHEIFSRGQMPYPGMSNHETFLRVDAGYRMPCPLECPPNIHKLMLSCWSRDPKQ 430 | ************************************************************ |
| S: 241 rPtfetlqevl 251 | rP+f+ l+e+l | Q: 431 RPCFKDLCEKL 441 | ******99875 |
|
Domain Sequence
(FASTA) | | TLCKKLGAGY FGEVFEALWK GQVHVAVKVI SRDNLLHQHT FQAEIQAMKK LRHKHILSLY 60 | | AVATAGDPVY IITELMPKGN LLQLLRDSDE KALPILELVD FASQVAEGMC YLESQNYIHR 120 | | DLAARNVLVT ENNLCKVGDF GLARLVKEDI YLSHEHNVPY KWTAPEALSR GHYSIKSDVW 180 | | SFGVLLHEIF SRGQMPYPGM SNHETFLRVD AGYRMPCPLE CPPNIHKLML SCWSRDPKQR 240 | | PCFKDLCEKL 250 |
|
| Keyword | ATP-binding; Cell projection; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
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| Pfam | |
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| Prints | |
| Created Date | 20-Feb-2013 |